pycom11g25220

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
28053892 .. 28054158
267 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 267 bp
ATGGAGCTGAGCAAGGTGACCTTAGAGATCTTCACGAAGCTGGAGCAGAAGTGGCTCTCCCACTGCGAAACCGCCAAGAAGACTCGGATTCTTAGCATCGACGGCGGAGGCACAACCGGCATTGTCGCCGGAGCCGCCTTGATCCACCTCGAGCGATCAGATCCGACTCAAAACCGGAGATTCCCATGCTCAAATCGCCGATTACTTCGACCTCGTCACCGGTACCGGCATCGGTGCCGTCCTCGCCGCCATGCTCGTCGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

89

Amino Acids

10.4

Weight (kDa)

11.64

Isoelectric Point (pI)

61.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 222
AccB1I GGYRCC 2 cut(s) 222, 234
AciI CCGC 4 cut(s) 72, 105, 135, 247
AclWI GGATC 2 cut(s) 136, 155
AfaI GTAC 1 cut(s) 224
AgeI ACCGGT 1 cut(s) 219
AluBI AGCT 2 cut(s) 7, 40
AluI AGCT 2 cut(s) 7, 40
AlwI GGATC 2 cut(s) 136, 155
Ama87I CYCGRG 1 cut(s) 149
AsiGI ACCGGT 1 cut(s) 219
Asp718I GGTACC 1 cut(s) 222
AsuHPI GGTGA 2 cut(s) 28, 209
AvaI CYCGRG 1 cut(s) 149
BanI GGYRCC 2 cut(s) 222, 234
BbsI GAAGAC 1 cut(s) 86
BceAI ACGGC 2 cut(s) 118, 222
BglII AGATCT 1 cut(s) 27
BisI GCNGC 2 cut(s) 135, 247
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 2 cut(s) 136, 248
BmeT110I CYCGRG 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 133, 224, 236
BmsI GCATC 2 cut(s) 105, 238
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 8
BsaWI WCCGGW 2 cut(s) 174, 219
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bse118I RCCGGY 3 cut(s) 116, 219, 225
BshNI GGYRCC 2 cut(s) 222, 234
BshTI ACCGGT 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 5 cut(s) 117, 129, 175, 220, 226
BsoBI CYCGRG 1 cut(s) 149
Bsp143I GATC 4 cut(s) 27, 141, 155, 160
Bsp1720I GCTNAGC 1 cut(s) 8
BspACI CCGC 4 cut(s) 72, 105, 135, 247
BspLI GGNNCC 3 cut(s) 133, 224, 236
BspPI GGATC 2 cut(s) 136, 155
BspT107I GGYRCC 2 cut(s) 222, 234
BsrFI RCCGGY 3 cut(s) 116, 219, 225
BssAI RCCGGY 3 cut(s) 116, 219, 225
BssMI GATC 4 cut(s) 27, 141, 155, 160
BstDEI CTNAG 3 cut(s) 8, 22, 92
BstEII GGTNACC 1 cut(s) 16
BstKTI GATC 4 cut(s) 30, 144, 158, 163
BstMBI GATC 4 cut(s) 27, 141, 155, 160
BstMWI GCNNNNNNNGC 6 cut(s) 52, 102, 117, 134, 195, 243
BstPI GGTNACC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 27, 160
BstYI RGATCY 2 cut(s) 27, 160
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 1 cut(s) 61
Cfr10I RCCGGY 3 cut(s) 116, 219, 225
Csp6I GTAC 1 cut(s) 223
CspAI ACCGGT 1 cut(s) 219
CviAII CATG 2 cut(s) 186, 251
CviJI RGCY 4 cut(s) 7, 40, 55, 134
CviKI_1 RGCY 4 cut(s) 7, 40, 55, 134
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 3 cut(s) 8, 22, 92
DpnI GATC 4 cut(s) 29, 143, 157, 162
DpnII GATC 4 cut(s) 27, 141, 155, 160
EciI GGCGGA 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 149
Eco91I GGTNACC 1 cut(s) 16
EcoO65I GGTNACC 1 cut(s) 16
FaeI CATG 2 cut(s) 189, 254
FaiI YATR 2 cut(s) 187, 252
FalI AAGNNNNNCTT 1 cut(s) 37
FatI CATG 2 cut(s) 185, 250
Fnu4HI GCNGC 2 cut(s) 135, 247
Fsp4HI GCNGC 2 cut(s) 135, 247
GluI GCNGC 2 cut(s) 135, 247
GsuI CTGGAG 1 cut(s) 62
HapII CCGG 5 cut(s) 117, 129, 175, 220, 226
Hin1II CATG 2 cut(s) 189, 254
HinfI GANTC 4 cut(s) 82, 88, 166, 180
HpaII CCGG 5 cut(s) 117, 129, 175, 220, 226
HphI GGTGA 2 cut(s) 28, 209
Hpy188I TCNGA 3 cut(s) 87, 160, 165
Hpy188III TCNNGA 1 cut(s) 34
Hpy99I CGWCG 2 cut(s) 104, 261
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 102, 117, 134, 195, 243
HpyF3I CTNAG 3 cut(s) 8, 22, 92
Hsp92II CATG 2 cut(s) 189, 254
KpnI GGTACC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 27, 141, 155, 160
LmnI GCTCC 3 cut(s) 4, 43, 131
LpnPI CCDG 6 cut(s) 26, 130, 142, 188, 233, 239
LweI GCATC 2 cut(s) 105, 238
MaeIII GTNAC 2 cut(s) 16, 215
MalI GATC 4 cut(s) 29, 143, 157, 162
MboI GATC 4 cut(s) 27, 141, 155, 160
MboII GAAGA 2 cut(s) 22, 91
MflI RGATCY 2 cut(s) 27, 160
MlyI GAGTC 2 cut(s) 76, 160
MmeI TCCRAC 1 cut(s) 188
MnlI CCTC 4 cut(s) 101, 158, 222, 252
MspI CCGG 5 cut(s) 117, 129, 175, 220, 226
MwoI GCNNNNNNNGC 6 cut(s) 52, 102, 117, 134, 195, 243
NdeII GATC 4 cut(s) 27, 141, 155, 160
NlaIII CATG 2 cut(s) 189, 254
NlaIV GGNNCC 3 cut(s) 133, 224, 236
NmuCI GTSAC 2 cut(s) 16, 215
PaeR7I CTCGAG 1 cut(s) 149
PfeI GAWTC 2 cut(s) 88, 180
PflFI GACNNNGTC 1 cut(s) 213
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 2 cut(s) 136, 248
PleI GAGTC 2 cut(s) 76, 160
PpsI GAGTC 2 cut(s) 76, 160
PspEI GGTNACC 1 cut(s) 16
PspN4I GGNNCC 3 cut(s) 133, 224, 236
PspXI VCTCGAGB 1 cut(s) 149
PsuI RGATCY 2 cut(s) 27, 160
PsyI GACNNNGTC 1 cut(s) 213
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SatI GCNGC 2 cut(s) 135, 247
Sau3AI GATC 4 cut(s) 27, 141, 155, 160
SchI GAGTC 2 cut(s) 76, 160
SetI ASST 6 cut(s) 9, 18, 23, 42, 150, 214
SfaNI GCATC 2 cut(s) 105, 238
Sfr274I CTCGAG 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SsiI CCGC 4 cut(s) 72, 105, 135, 247
TaqI TCGA 3 cut(s) 99, 150, 208
TauI GCSGC 2 cut(s) 137, 249
TfiI GAWTC 2 cut(s) 88, 180
TscAI CASTG 1 cut(s) 68
TseFI GTSAC 2 cut(s) 16, 215
Tsp45I GTSAC 2 cut(s) 16, 215
TspRI CASTG 1 cut(s) 68
Tth111I GACNNNGTC 1 cut(s) 213
XhoI CTCGAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.