pycom11g25230

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Forward (+)
28054268 .. 28055137
870 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGTTTTCCGGTGGGAGCATGGAGAAGGCGTTGGCGGAGCTTCTGACGAGGGAGGACGGGAAGGTTCTGACGCTCAAGGACACGTGTAAGCCTCTCCTGGTTCCCTGCTACGACATGAAGAGCTCCGCCCCGTTCGTGTTCTCCCGAGCCGACGCGTCCGAGTCGGCGAGTTTCGACTTCGAGCTGTGGAAAGTCTGCCGCGCCACGTCATCGACTCCGGGCGTGTTCAAGCCGTTCAGCCTGAGATCCGTGGACGGAAAGACTTCGTGCTCGGCCGTGGACGGCGGTCTTGTGATGAACAACCCGACGGCGGCGGCTGTTACGCACGTGCTCAACAACAAGCGCGACTTCCCCTCCGTCAATGGCGTCGAGGACCTGCTAGTTTTGTCGTTAGGTAACGGCCCGCTGAGCGGAGAGAAGGCGGGGCGGGGTAACGGAAAGTGCTCGCCGCCGTCGGTGGTTGACATTGTGCTCGACGGAGTTTCCGAGACCATTGACCAGATGATGGGGAACGCCTTCTGTTATAACCGCGCTGATTACGTCAGAATTCAGGCGTTCGGATTGGGGAGCGAAGGGGTTGCGGGCCAGCGATCGGAGGGGGAAGCGGCGGTGTTGAAGGAGAGAGGGGTCGAGTCGTTACCGTTTGGCGGGAAGCGGTTATTGACGGAGACAAACGGAGAGAGAATCGAGGGTTTCGTGCAACGACTCGTGGCTTGTGGAAGGAGCAGTCTGCCACCTAGTCCCTGCAAGGATTCCACCGTTAGCCCTGTCGTTAACGGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

262

Amino Acids

27.59

Weight (kDa)

5.99

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 23 - 106 1.1e-10 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 384
AccB7I CCANNNNNTGG 1 cut(s) 505
AccBSI CCGCTC 1 cut(s) 411
AccII CGCG 4 cut(s) 155, 201, 345, 531
AclWI GGATC 1 cut(s) 240
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 1 cut(s) 546
AcvI CACGTG 2 cut(s) 84, 328
AcyI GRCGYC 1 cut(s) 366
AfiI CCNNNNNNNGG 5 cut(s) 310, 410, 505, 592, 647
AflIII ACRYGT 3 cut(s) 81, 83, 153
AgsI TTSAA 2 cut(s) 229, 616
AjiI CACGTC 1 cut(s) 207
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 3 cut(s) 40, 123, 184
AluI AGCT 3 cut(s) 40, 123, 184
Alw21I GWGCWC 5 cut(s) 125, 272, 333, 446, 474
Alw26I GTCTC 2 cut(s) 482, 662
AlwI GGATC 1 cut(s) 240
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 273, 400, 583
ApoI RAATTY 1 cut(s) 546
Asp700I GAANNNNTTC 2 cut(s) 262, 515
AspLEI GCGC 3 cut(s) 203, 345, 533
AspS9I GGNCC 3 cut(s) 373, 401, 583
AsuC2I CCSGG 1 cut(s) 219
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 125
BauI CACGAG 1 cut(s) 707
BbrPI CACGTG 2 cut(s) 84, 328
Bbv12I GWGCWC 5 cut(s) 125, 272, 333, 446, 474
BccI CCATC 1 cut(s) 499
BceAI ACGGC 6 cut(s) 217, 260, 298, 324, 415, 436
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 1 cut(s) 98
BcnI CCSGG 1 cut(s) 219
BcoDI GTCTC 2 cut(s) 482, 662
BfaI CTAG 2 cut(s) 380, 738
BfuAI ACCTGC 1 cut(s) 384
BisI GCNGC 5 cut(s) 199, 312, 315, 449, 606
BlpI GCTNAGC 1 cut(s) 407
BlsI GCNGC 5 cut(s) 200, 313, 316, 450, 607
Bme1390I CCNGG 2 cut(s) 98, 219
Bme18I GGWCC 1 cut(s) 373
BmeT110I CYCGRG 1 cut(s) 144
BmgBI CACGTC 1 cut(s) 207
BmgT120I GGNCC 3 cut(s) 373, 401, 583
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 2 cut(s) 98, 219
BoxI GACNNNNGTC 1 cut(s) 285
Bpu1102I GCTNAGC 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 219
BsaAI YACGTR 2 cut(s) 84, 328
BsaHI GRCGYC 1 cut(s) 366
BsaI GGTCTC 1 cut(s) 482
BsaJI CCNNGG 2 cut(s) 249, 276
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 338, 368
Bsc4I CCNNNNNNNGG 5 cut(s) 310, 410, 505, 592, 647
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 249, 276
BseLI CCNNNNNNNGG 5 cut(s) 310, 410, 505, 592, 647
BseMII CTCAG 2 cut(s) 233, 398
BseX3I CGGCCG 1 cut(s) 273
Bsh1236I CGCG 4 cut(s) 155, 201, 345, 531
Bsh1285I CGRYCG 3 cut(s) 276, 593, 781
BshFI GGCC 3 cut(s) 275, 402, 585
BsiEI CGRYCG 3 cut(s) 276, 593, 781
BsiHKAI GWGCWC 5 cut(s) 125, 272, 333, 446, 474
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 2 cut(s) 9, 218
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 5 cut(s) 310, 410, 505, 592, 647
BsmAI GTCTC 2 cut(s) 482, 662
BsmFI GGGAC 1 cut(s) 726
BsnI GGCC 3 cut(s) 275, 402, 585
Bso31I GGTCTC 1 cut(s) 482
BsoBI CYCGRG 1 cut(s) 144
Bsp1286I GDGCHC 5 cut(s) 125, 272, 333, 446, 474
Bsp143I GATC 2 cut(s) 245, 590
Bsp1720I GCTNAGC 1 cut(s) 407
BspANI GGCC 3 cut(s) 275, 402, 585
BspCNI CTCAG 2 cut(s) 234, 399
BspFNI CGCG 4 cut(s) 155, 201, 345, 531
BspLI GGNNCC 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 384
BspPI GGATC 1 cut(s) 240
BspQI GCTCTTC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 482
BsrBI CCGCTC 1 cut(s) 411
BssECI CCNNGG 2 cut(s) 249, 276
BssMI GATC 2 cut(s) 245, 590
BssNI GRCGYC 1 cut(s) 366
BssSI CACGAG 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 3 cut(s) 642, 760, 779
Bst6I CTCTTC 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 366
BstBAI YACGTR 2 cut(s) 84, 328
BstC8I GCNNGC 4 cut(s) 404, 446, 583, 587
BstDEI CTNAG 2 cut(s) 242, 407
BstDSI CCRYGG 2 cut(s) 249, 276
BstFNI CGCG 4 cut(s) 155, 201, 345, 531
BstHHI GCGC 3 cut(s) 203, 345, 533
BstKTI GATC 2 cut(s) 248, 593
BstMAI GTCTC 2 cut(s) 482, 662
BstMBI GATC 2 cut(s) 245, 590
BstMCI CGRYCG 3 cut(s) 276, 593, 781
BstMWI GCNNNNNNNGC 1 cut(s) 408
BstNI CCWGG 1 cut(s) 98
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 2 cut(s) 96, 217
BstUI CGCG 4 cut(s) 155, 201, 345, 531
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 3 cut(s) 275, 402, 585
BtgI CCRYGG 2 cut(s) 249, 276
BtrI CACGTC 1 cut(s) 207
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 4 cut(s) 404, 446, 583, 587
CfoI GCGC 3 cut(s) 203, 345, 533
Cfr13I GGNCC 3 cut(s) 373, 401, 583
CseI GACGC 4 cut(s) 79, 144, 161, 355
CviAII CATG 2 cut(s) 19, 115
DdeI CTNAG 2 cut(s) 242, 407
DpnI GATC 2 cut(s) 247, 592
DpnII GATC 2 cut(s) 245, 590
EaeI YGGCCR 1 cut(s) 273
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 2 cut(s) 50, 115
Ecl136II GAGCTC 1 cut(s) 123
EclXI CGGCCG 1 cut(s) 273
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 373
Eco52I CGGCCG 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 123
Eco72I CACGTG 2 cut(s) 84, 328
Eco88I CYCGRG 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 1 cut(s) 373
EcoRI GAATTC 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 96
EcoT38I GRGCYC 1 cut(s) 125
FaeI CATG 2 cut(s) 22, 118
FaiI YATR 3 cut(s) 20, 116, 525
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 1 cut(s) 726
FatI CATG 2 cut(s) 18, 114
FauI CCCGC 5 cut(s) 411, 415, 420, 574, 641
Fnu4HI GCNGC 5 cut(s) 199, 312, 315, 449, 606
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 5 cut(s) 199, 312, 315, 449, 606
FspBI CTAG 2 cut(s) 380, 738
GlaI GCGC 3 cut(s) 202, 344, 532
GluI GCNGC 5 cut(s) 199, 312, 315, 449, 606
HaeIII GGCC 3 cut(s) 275, 402, 585
HapII CCGG 2 cut(s) 9, 218
HgaI GACGC 4 cut(s) 79, 144, 161, 355
HhaI GCGC 3 cut(s) 203, 345, 533
Hin1I GRCGYC 1 cut(s) 366
Hin1II CATG 2 cut(s) 22, 118
Hin6I GCGC 3 cut(s) 201, 343, 531
HinP1I GCGC 3 cut(s) 201, 343, 531
HincII GTYRAC 2 cut(s) 463, 775
HindII GTYRAC 2 cut(s) 463, 775
HinfI GANTC 6 cut(s) 161, 214, 632, 684, 705, 752
HpaI GTTAAC 1 cut(s) 775
HpaII CCGG 2 cut(s) 9, 218
Hpy166II GTNNAC 4 cut(s) 253, 280, 463, 775
Hpy188I TCNGA 7 cut(s) 45, 69, 160, 487, 545, 560, 595
Hpy188III TCNNGA 1 cut(s) 144
Hpy8I GTNNAC 4 cut(s) 253, 280, 463, 775
Hpy99I CGWCG 5 cut(s) 155, 310, 371, 457, 479
HpyAV CCTTC 7 cut(s) 19, 55, 412, 526, 566, 610, 714
HpyCH4III ACNGT 3 cut(s) 642, 760, 779
HpyCH4IV ACGT 4 cut(s) 83, 206, 327, 540
HpyCH4V TGCA 2 cut(s) 700, 747
HpyF10VI GCNNNNNNNGC 1 cut(s) 408
HpyF3I CTNAG 2 cut(s) 242, 407
HpySE526I ACGT 4 cut(s) 83, 206, 327, 540
Hsp92I GRCGYC 1 cut(s) 366
Hsp92II CATG 2 cut(s) 22, 118
HspAI GCGC 3 cut(s) 201, 343, 531
KspAI GTTAAC 1 cut(s) 775
Kzo9I GATC 2 cut(s) 245, 590
LguI GCTCTTC 1 cut(s) 113
LmnI GCTCC 5 cut(s) 15, 37, 128, 567, 723
MaeI CTAG 2 cut(s) 380, 738
MaeII ACGT 4 cut(s) 83, 206, 327, 540
MaeIII GTNAC 4 cut(s) 319, 395, 431, 636
MalI GATC 2 cut(s) 247, 592
MbiI CCGCTC 1 cut(s) 411
MboI GATC 2 cut(s) 245, 590
MboII GAAGA 1 cut(s) 130
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 5 cut(s) 125, 272, 333, 446, 474
MluCI AATT 1 cut(s) 546
MluI ACGCGT 1 cut(s) 153
MlyI GAGTC 4 cut(s) 170, 208, 641, 699
MnlI CCTC 8 cut(s) 42, 46, 102, 364, 364, 589, 617, 682
MroXI GAANNNNTTC 2 cut(s) 262, 515
MseI TTAA 1 cut(s) 774
MspA1I CMGCKG 1 cut(s) 406
MspI CCGG 2 cut(s) 9, 218
MspR9I CCNGG 2 cut(s) 98, 219
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 4 cut(s) 155, 201, 345, 531
MwoI GCNNNNNNNGC 1 cut(s) 408
NciI CCSGG 1 cut(s) 219
NdeII GATC 2 cut(s) 245, 590
NlaIII CATG 2 cut(s) 22, 118
NlaIV GGNNCC 1 cut(s) 102
NmeAIII GCCGAG 1 cut(s) 251
PciSI GCTCTTC 1 cut(s) 113
PcsI WCGNNNNNNNCGW 2 cut(s) 483, 693
PdmI GAANNNNTTC 2 cut(s) 262, 515
PfeI GAWTC 2 cut(s) 684, 752
PflMI CCANNNNNTGG 1 cut(s) 505
PkrI GCNGC 5 cut(s) 200, 313, 316, 450, 607
Ple19I CGATCG 1 cut(s) 593
PleI GAGTC 4 cut(s) 169, 208, 640, 699
PmaCI CACGTG 2 cut(s) 84, 328
PmlI CACGTG 2 cut(s) 84, 328
PpsI GAGTC 4 cut(s) 169, 208, 640, 699
Ppu21I YACGTR 2 cut(s) 84, 328
PpuMI RGGWCCY 1 cut(s) 373
PshAI GACNNNNGTC 1 cut(s) 285
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 2 cut(s) 84, 328
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 3 cut(s) 373, 401, 583
PspPPI RGGWCCY 1 cut(s) 373
PsuI RGATCY 1 cut(s) 245
PvuI CGATCG 1 cut(s) 593
SacI GAGCTC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 5 cut(s) 199, 312, 315, 449, 606
Sau3AI GATC 2 cut(s) 245, 590
Sau96I GGNCC 3 cut(s) 373, 401, 583
SchI GAGTC 4 cut(s) 170, 208, 641, 699
ScrFI CCNGG 2 cut(s) 98, 219
SduI GDGCHC 5 cut(s) 125, 272, 333, 446, 474
SinI GGWCC 1 cut(s) 373
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 1 cut(s) 546
SspMI CTAG 2 cut(s) 380, 738
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 2 cut(s) 96, 217
TaaI ACNGT 3 cut(s) 642, 760, 779
TaiI ACGT 4 cut(s) 86, 209, 330, 543
TaqI TCGA 7 cut(s) 174, 180, 212, 369, 474, 630, 687
TasI AATT 1 cut(s) 546
TauI GCSGC 5 cut(s) 201, 314, 317, 451, 608
TfiI GAWTC 2 cut(s) 684, 752
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TspDTI ATGAA 2 cut(s) 131, 311
TspGWI ACGGA 7 cut(s) 238, 270, 346, 450, 492, 680, 690
Van91I CCANNNNNTGG 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 546
XmnI GAANNNNTTC 2 cut(s) 262, 515
XspI CTAG 2 cut(s) 380, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.