pycom11g25560

R3H-associated N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
N/A
Physical Location & Seq
Reverse (-)
28346700 .. 28347768
1069 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGGCCCACAATCACATTCCTCAGACAGAGATTACAAAAATTAACATAGAATGTCACAAATGTCAAGAGAAGCTCAAGAGATTCCTCTCAAAATTTCAGGGAACCTATTCTGTTAAGTTCGACCCGGAATCCGGAAGATTAACAGTTGAATCCACAGTAGACAAGAACACGATCAATGAGGCAGTAGAGCATGCTAGGTTTTCTGCAGGGCTCATGTGTGAGGATAATAGTCTGCCGCCATCCCACAACAGTTTCTTGCAACCCAAGGCAACCGCCAGCCACGAGTTGAACGGAGCACAAGATCAAGGGCTTGAGGTAATAAGATGGGCTCAAATGGGAGAGCTCCAAAAACTTCCCAACATCCACAGGCTAAAGAAGTTGGAGCTGACTCAATCTGGAAGCAATCGAAGAATCAAACTCACATTCTTCGACAAGAAACCTCAGCCTCAGCCACAACCTCAGCCCCATGCTAGGGTTAATGTCATTAATGTTAAGGATGATGAGCAAAATGAGCCCCATGGGGGAGGAAGTGGTGGCAATAGTTGGCCGGCGGACAAAGTTTGTGGAGATCATAAAGGCGGCGAAGGAAGCAGCACCACAAGTTGTGATGGTGGTAGCCACCACAACTTTGAGTTTCAATACGTTCATACTCCTGAGGCAGCAGGCAATAATAATAATCATCATGTGCCACATGATACTTATTTGCCTCCGACGTACGGTGACCGTGTGACGGTGGAACACGCTGCGGTCCGGTGCGAGGAGACCATTGCAAACAGATGCGTCATCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
R3H R3H-assoc

Protein Analysis

264

Amino Acids

29.26

Weight (kDa)

6.49

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 12 - 66 1.2e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 159
AccIII TCCGGA 1 cut(s) 131
AciI CCGC 5 cut(s) 236, 273, 551, 579, 746
AcoI YGGCCR 1 cut(s) 545
AcsI RAATTY 1 cut(s) 92
AfaI GTAC 1 cut(s) 716
AfiI CCNNNNNNNGG 7 cut(s) 131, 471, 472, 521, 716, 730, 757
AgsI TTSAA 3 cut(s) 149, 289, 638
AluBI AGCT 3 cut(s) 73, 343, 385
AluI AGCT 3 cut(s) 73, 343, 385
Alw21I GWGCWC 2 cut(s) 298, 345
Alw26I GTCTC 1 cut(s) 755
Aor13HI TCCGGA 1 cut(s) 131
AoxI GGCC 2 cut(s) 3, 545
ApeKI GCWGC 3 cut(s) 591, 659, 743
ApoI RAATTY 1 cut(s) 92
AseI ATTAAT 1 cut(s) 486
Asp700I GAANNNNTTC 1 cut(s) 106
AspS9I GGNCC 2 cut(s) 4, 748
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 1 cut(s) 731
AvaII GGWCC 1 cut(s) 748
AxyI CCTNAGG 1 cut(s) 654
BanII GRGCYC 4 cut(s) 213, 331, 345, 516
BauI CACGAG 1 cut(s) 281
Bbv12I GWGCWC 2 cut(s) 298, 345
BbvCI CCTCAGC 3 cut(s) 441, 447, 459
BbvI GCAGC 3 cut(s) 603, 671, 730
BccI CCATC 3 cut(s) 247, 318, 602
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 755
BfaI CTAG 2 cut(s) 195, 471
BfmI CTRYAG 1 cut(s) 204
BisI GCNGC 5 cut(s) 236, 580, 592, 660, 744
BlsI GCNGC 5 cut(s) 237, 581, 593, 661, 745
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 1 cut(s) 748
BmgT120I GGNCC 2 cut(s) 4, 748
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 767
Bpu10I CCTNAGC 3 cut(s) 441, 447, 459
BpuEI CTTGAG 2 cut(s) 59, 332
BpuMI CCSGG 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 755
BsaJI CCNNGG 2 cut(s) 264, 517
BsaWI WCCGGW 2 cut(s) 131, 750
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 471, 472, 521, 716, 730, 757
Bse118I RCCGGY 1 cut(s) 547
Bse21I CCTNAGG 1 cut(s) 654
Bse3DI GCAATG 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 264, 517
BseGI GGATG 3 cut(s) 239, 360, 502
BseLI CCNNNNNNNGG 7 cut(s) 131, 471, 472, 521, 716, 730, 757
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 5 cut(s) 35, 455, 461, 473, 645
BseRI GAGGAG 1 cut(s) 773
BseXI GCAGC 3 cut(s) 603, 671, 730
BshFI GGCC 2 cut(s) 5, 547
BsiHKAI GWGCWC 2 cut(s) 298, 345
BsiSI CCGG 4 cut(s) 125, 132, 548, 751
BsiWI CGTACG 1 cut(s) 714
BslI CCNNNNNNNGG 7 cut(s) 131, 471, 472, 521, 716, 730, 757
BsmAI GTCTC 1 cut(s) 755
BsnI GGCC 2 cut(s) 5, 547
Bso31I GGTCTC 1 cut(s) 755
Bsp1286I GDGCHC 5 cut(s) 213, 298, 331, 345, 516
Bsp13I TCCGGA 1 cut(s) 131
Bsp143I GATC 3 cut(s) 171, 301, 568
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 5 cut(s) 236, 273, 551, 579, 746
BspANI GGCC 2 cut(s) 5, 547
BspCNI CTCAG 5 cut(s) 34, 454, 460, 472, 646
BspEI TCCGGA 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 103
BspMAI CTGCAG 1 cut(s) 208
BspTNI GGTCTC 1 cut(s) 755
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 547
BssAI RCCGGY 1 cut(s) 547
BssECI CCNNGG 2 cut(s) 264, 517
BssMI GATC 3 cut(s) 171, 301, 568
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 264, 517
Bst2BI CACGAG 1 cut(s) 281
Bst4CI ACNGT 6 cut(s) 145, 157, 251, 719, 725, 733
BstC8I GCNNGC 4 cut(s) 192, 277, 549, 664
BstDEI CTNAG 5 cut(s) 21, 441, 447, 459, 654
BstDSI CCRYGG 1 cut(s) 517
BstEII GGTNACC 1 cut(s) 719
BstF5I GGATG 3 cut(s) 239, 360, 502
BstKTI GATC 3 cut(s) 174, 304, 571
BstMAI GTCTC 1 cut(s) 755
BstMBI GATC 3 cut(s) 171, 301, 568
BstMWI GCNNNNNNNGC 2 cut(s) 511, 588
BstNSI RCATGY 1 cut(s) 194
BstPI GGTNACC 1 cut(s) 719
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 603, 671, 730
Bsu36I CCTNAGG 1 cut(s) 654
BsuRI GGCC 2 cut(s) 5, 547
BtgI CCRYGG 1 cut(s) 517
BtsCI GGATG 3 cut(s) 239, 360, 502
Cac8I GCNNGC 4 cut(s) 192, 277, 549, 664
Cfr10I RCCGGY 1 cut(s) 547
Cfr13I GGNCC 2 cut(s) 4, 748
CpoI CGGWCCG 1 cut(s) 748
CseI GACGC 1 cut(s) 769
Csp6I GTAC 1 cut(s) 715
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
CspI CGGWCCG 1 cut(s) 748
CviAII CATG 6 cut(s) 191, 214, 467, 518, 683, 692
CviQI GTAC 1 cut(s) 715
DdeI CTNAG 5 cut(s) 21, 441, 447, 459, 654
DpnI GATC 3 cut(s) 173, 303, 570
DpnII GATC 3 cut(s) 171, 301, 568
EaeI YGGCCR 1 cut(s) 545
EciI GGCGGA 1 cut(s) 566
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 2 cut(s) 264, 517
Eco24I GRGCYC 4 cut(s) 213, 331, 345, 516
Eco31I GGTCTC 1 cut(s) 755
Eco47I GGWCC 1 cut(s) 748
Eco53kI GAGCTC 1 cut(s) 343
Eco81I CCTNAGG 1 cut(s) 654
Eco91I GGTNACC 1 cut(s) 719
EcoICRI GAGCTC 1 cut(s) 343
EcoO65I GGTNACC 1 cut(s) 719
EcoT14I CCWWGG 2 cut(s) 264, 517
EcoT38I GRGCYC 4 cut(s) 213, 331, 345, 516
ErhI CCWWGG 2 cut(s) 264, 517
FaeI CATG 6 cut(s) 194, 217, 470, 521, 686, 695
FaiI YATR 9 cut(s) 47, 192, 215, 468, 519, 573, 648, 684, 693
FatI CATG 6 cut(s) 190, 213, 466, 517, 682, 691
FblI GTMKAC 1 cut(s) 159
Fnu4HI GCNGC 5 cut(s) 236, 580, 592, 660, 744
FokI GGATG 3 cut(s) 226, 347, 509
FriOI GRGCYC 4 cut(s) 213, 331, 345, 516
Fsp4HI GCNGC 5 cut(s) 236, 580, 592, 660, 744
FspBI CTAG 2 cut(s) 195, 471
GluI GCNGC 5 cut(s) 236, 580, 592, 660, 744
HaeIII GGCC 2 cut(s) 5, 547
HapII CCGG 4 cut(s) 125, 132, 548, 751
HgaI GACGC 1 cut(s) 769
Hin1II CATG 6 cut(s) 194, 217, 470, 521, 686, 695
HinfI GANTC 5 cut(s) 81, 128, 149, 388, 411
HpaII CCGG 4 cut(s) 125, 132, 548, 751
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 24, 711
Hpy188III TCNNGA 5 cut(s) 65, 76, 132, 396, 653
Hpy8I GTNNAC 1 cut(s) 160
Hpy99I CGWCG 1 cut(s) 715
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 6 cut(s) 145, 157, 251, 719, 725, 733
HpyCH4IV ACGT 2 cut(s) 642, 713
HpyCH4V TGCA 3 cut(s) 206, 259, 770
HpyF10VI GCNNNNNNNGC 2 cut(s) 511, 588
HpyF3I CTNAG 5 cut(s) 21, 441, 447, 459, 654
HpySE526I ACGT 2 cut(s) 642, 713
Hsp92II CATG 6 cut(s) 194, 217, 470, 521, 686, 695
Kpn2I TCCGGA 1 cut(s) 131
KroI GCCGGC 1 cut(s) 547
KroNI GCCGGC 1 cut(s) 549
Kzo9I GATC 3 cut(s) 171, 301, 568
LmnI GCTCC 3 cut(s) 293, 348, 382
Lsp1109I GCAGC 3 cut(s) 603, 671, 730
LweI GCATC 1 cut(s) 767
MaeI CTAG 2 cut(s) 195, 471
MaeII ACGT 2 cut(s) 642, 713
MaeIII GTNAC 3 cut(s) 53, 719, 727
MalI GATC 3 cut(s) 173, 303, 570
MboI GATC 3 cut(s) 171, 301, 568
MboII GAAGA 3 cut(s) 147, 418, 420
MhlI GDGCHC 5 cut(s) 213, 298, 331, 345, 516
MluCI AATT 2 cut(s) 39, 92
MlyI GAGTC 1 cut(s) 382
MmeI TCCRAC 2 cut(s) 360, 734
MroI TCCGGA 1 cut(s) 131
MroNI GCCGGC 1 cut(s) 547
MroXI GAANNNNTTC 1 cut(s) 106
MseI TTAA 6 cut(s) 42, 114, 140, 477, 486, 492
MspI CCGG 4 cut(s) 125, 132, 548, 751
MspR9I CCNGG 1 cut(s) 125
MwoI GCNNNNNNNGC 2 cut(s) 511, 588
NaeI GCCGGC 1 cut(s) 549
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 517
NdeII GATC 3 cut(s) 171, 301, 568
NgoMIV GCCGGC 1 cut(s) 547
NlaIII CATG 6 cut(s) 194, 217, 470, 521, 686, 695
NlaIV GGNNCC 1 cut(s) 103
NmuCI GTSAC 3 cut(s) 53, 719, 727
NspI RCATGY 1 cut(s) 194
PaeI GCATGC 1 cut(s) 194
PdiI GCCGGC 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 106
PfeI GAWTC 4 cut(s) 81, 128, 149, 411
Pfl23II CGTACG 1 cut(s) 714
PkrI GCNGC 5 cut(s) 237, 581, 593, 661, 745
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 486
Psp124BI GAGCTC 1 cut(s) 345
PspEI GGTNACC 1 cut(s) 719
PspLI CGTACG 1 cut(s) 714
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 2 cut(s) 4, 748
PstI CTGCAG 1 cut(s) 208
RsaI GTAC 1 cut(s) 716
RsaNI GTAC 1 cut(s) 715
Rsr2I CGGWCCG 1 cut(s) 748
RsrII CGGWCCG 1 cut(s) 748
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 6 cut(s) 42, 114, 140, 477, 486, 492
SatI GCNGC 5 cut(s) 236, 580, 592, 660, 744
Sau3AI GATC 3 cut(s) 171, 301, 568
Sau96I GGNCC 2 cut(s) 4, 748
SchI GAGTC 1 cut(s) 382
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 5 cut(s) 213, 298, 331, 345, 516
SfaNI GCATC 1 cut(s) 767
SfcI CTRYAG 1 cut(s) 204
SinI GGWCC 1 cut(s) 748
SmlI CTYRAG 2 cut(s) 74, 311
SmoI CTYRAG 2 cut(s) 74, 311
SphI GCATGC 1 cut(s) 194
Sse9I AATT 2 cut(s) 39, 92
SsiI CCGC 5 cut(s) 236, 273, 551, 579, 746
SspMI CTAG 2 cut(s) 195, 471
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 2 cut(s) 264, 517
TaaI ACNGT 6 cut(s) 145, 157, 251, 719, 725, 733
TaiI ACGT 2 cut(s) 645, 716
TaqI TCGA 3 cut(s) 120, 406, 429
TasI AATT 2 cut(s) 39, 92
TauI GCSGC 2 cut(s) 238, 582
TfiI GAWTC 4 cut(s) 81, 128, 149, 411
Tru1I TTAA 6 cut(s) 42, 114, 140, 477, 486, 492
Tru9I TTAA 6 cut(s) 42, 114, 140, 477, 486, 492
TseFI GTSAC 3 cut(s) 53, 719, 727
TseI GCWGC 3 cut(s) 591, 659, 743
Tsp45I GTSAC 3 cut(s) 53, 719, 727
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 1 cut(s) 306
VpaK11BI GGWCC 1 cut(s) 748
VspI ATTAAT 1 cut(s) 486
XapI RAATTY 1 cut(s) 92
XceI RCATGY 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 159
XmnI GAANNNNTTC 1 cut(s) 106
XspI CTAG 2 cut(s) 195, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.