pycom11g27140

pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0029250
Physical Location & Seq
Forward (+)
29496604 .. 29500009
3406 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 891 bp
ATGCACTCCGTGGTCGTGATGGCTATGATTTTGATGGGCATCGTTTACGGGTTGAACTTGCACATGGAGGGCGTGGGCATTCATCTTCTTCAGATCGATCAAAGTAACTATAGTGGTGGTCGAGGTGGACGTGGGATGTCTAGGCGCTCAGATTATCGTGTGTTAGTCAGTGAACTTCCCCCTTCCGCTTCATGGCAAGATCTTAAGGATCACATGCGACGGGCAGGAGATGTCTGTTTCTCCCAAGTTTTTCGTGATGGTAGTGGCACAACTGGGATTGTGGATTACACGAACTTAGAAGATATGAAGTATGCGATCAAAAAGCTTGATGGCTCTGAGTTCCGGAATGCTTTTGACCGCCAATCTGTTCGTGTGAGGGAATATGATGCGAAGAGGGACTCATCTAGGAGCCCTAGTCGCGGTCGATCTCATTCAAAAGGCAGGAGCTACAGTCGCAGTCGTAGTCCAAGTTATAGCCGGGACAGAAGCAGGAGCAAGTCTCCAAAAACCAAATCTTCACGCCGCTCACTTGCTAGATCTCGATCAAGATCTGCTTCTCTCCCCGGTTCACGATCAAGGTCTCGCTCTCCATCAGGATCAAGATCAAGAAGCAAATCTCCAGTGCCTTCAAAACGCATAAGTAAAAGCCCGAAGAAGCACCTTAGTAAAAGTCCAAAGAAGCGTAGTGCCAGCAGGAGTCCAATCCGGAGCAGGAGCAGGAGCAAGAGTTTGTCACGGTATCATTCTCGAGCATTATTTTTACTTTTGTTAGTGTCATCAGTGTCGCACCATCTTTTTCTTCTCGACACATTTTCCTCGAATTGCTATTGCTTTGTGAACGTTGAACTTGTGCAACAATTTGTTTTAGACTCTGATTGCAATTCTATTTGA

Protein Analysis

297

Amino Acids

33.32

Weight (kDa)

11.14

Isoelectric Point (pI)

95.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 54 - 116 2.1e-11 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0029250)

Species Orthologous Gene IDs
pyrus_communis pycom03g22760 pycom11g27140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 525
AccII CGCG 1 cut(s) 420
AccIII TCCGGA 2 cut(s) 342, 705
AciI CCGC 4 cut(s) 186, 358, 420, 523
AclI AACGTT 1 cut(s) 840
AclWI GGATC 2 cut(s) 216, 604
AcuI CTGAAG 1 cut(s) 74
AdeI CACNNNGTG 1 cut(s) 10
AfiI CCNNNNNNNGG 2 cut(s) 192, 419
AflII CTTAAG 1 cut(s) 203
AgsI TTSAA 4 cut(s) 55, 435, 630, 845
AjiI CACGTC 1 cut(s) 131
AluBI AGCT 2 cut(s) 325, 447
AluI AGCT 2 cut(s) 325, 447
Alw26I GTCTC 2 cut(s) 504, 585
AlwI GGATC 2 cut(s) 216, 604
Ama87I CYCGRG 1 cut(s) 747
Aor13HI TCCGGA 2 cut(s) 342, 705
AspLEI GCGC 1 cut(s) 147
AsuC2I CCSGG 2 cut(s) 479, 564
AvaI CYCGRG 1 cut(s) 747
BanII GRGCYC 1 cut(s) 413
BccI CCATC 6 cut(s) 13, 28, 251, 323, 598, 798
BcnI CCSGG 2 cut(s) 479, 564
BcoDI GTCTC 2 cut(s) 504, 585
BfaI CTAG 4 cut(s) 141, 405, 414, 534
BfmI CTRYAG 2 cut(s) 109, 448
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 203
BglII AGATCT 3 cut(s) 199, 536, 548
BisI GCNGC 1 cut(s) 523
BlsI GCNGC 1 cut(s) 524
Bme1390I CCNGG 2 cut(s) 479, 564
BmeT110I CYCGRG 1 cut(s) 747
BmgBI CACGTC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 410
BmrFI CCNGG 2 cut(s) 479, 564
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 2 cut(s) 48, 376
BmuI ACTGGG 1 cut(s) 282
BplI GAGNNNNNCTC 2 cut(s) 484, 516
BpmI CTGGAG 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 479, 564
Bsa29I ATCGAT 1 cut(s) 96
BsaBI GATNNNNATC 4 cut(s) 38, 541, 547, 601
BsaI GGTCTC 1 cut(s) 585
BsaJI CCNNGG 2 cut(s) 9, 562
BsaWI WCCGGW 2 cut(s) 342, 705
BsaXI ACNNNNNCTCC 6 cut(s) 400, 430, 436, 466, 712, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 192, 419
Bse1I ACTGG 2 cut(s) 277, 620
Bse8I GATNNNNATC 4 cut(s) 38, 541, 547, 601
BseAI TCCGGA 2 cut(s) 342, 705
BseCI ATCGAT 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 9, 562
BseGI GGATG 1 cut(s) 141
BseJI GATNNNNATC 4 cut(s) 38, 541, 547, 601
BseLI CCNNNNNNNGG 2 cut(s) 192, 419
BseMII CTCAG 2 cut(s) 162, 327
BseNI ACTGG 2 cut(s) 277, 620
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 96
BsiEI CGRYCG 1 cut(s) 424
BsiHKCI CYCGRG 1 cut(s) 747
BsiSI CCGG 4 cut(s) 343, 478, 564, 706
BslFI GGGAC 2 cut(s) 410, 494
BslI CCNNNNNNNGG 2 cut(s) 192, 419
BsmAI GTCTC 2 cut(s) 504, 585
BsmFI GGGAC 2 cut(s) 410, 494
BsmI GAATGC 2 cut(s) 78, 352
Bso31I GGTCTC 1 cut(s) 585
BsoBI CYCGRG 1 cut(s) 747
Bsp1286I GDGCHC 1 cut(s) 413
Bsp13I TCCGGA 2 cut(s) 342, 705
BspACI CCGC 4 cut(s) 186, 358, 420, 523
BspCNI CTCAG 2 cut(s) 161, 328
BspDI ATCGAT 1 cut(s) 96
BspEI TCCGGA 2 cut(s) 342, 705
BspFNI CGCG 1 cut(s) 420
BspLI GGNNCC 1 cut(s) 410
BspPI GGATC 2 cut(s) 216, 604
BspTI CTTAAG 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 585
BsrBI CCGCTC 1 cut(s) 525
BsrI ACTGG 2 cut(s) 277, 620
BssECI CCNNGG 2 cut(s) 9, 562
Bst4CI ACNGT 2 cut(s) 452, 738
Bst6I CTCTTC 1 cut(s) 386
BstAFI CTTAAG 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 691
BstDEI CTNAG 4 cut(s) 148, 295, 336, 662
BstDSI CCRYGG 1 cut(s) 9
BstF5I GGATG 1 cut(s) 141
BstFNI CGCG 1 cut(s) 420
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstMAI GTCTC 2 cut(s) 504, 585
BstMCI CGRYCG 1 cut(s) 424
BstMWI GCNNNNNNNGC 2 cut(s) 417, 453
BstNSI RCATGY 1 cut(s) 217
BstSCI CCNGG 2 cut(s) 477, 562
BstSFI CTRYAG 2 cut(s) 109, 448
BstUI CGCG 1 cut(s) 420
BstX2I RGATCY 3 cut(s) 199, 536, 548
BstYI RGATCY 3 cut(s) 199, 536, 548
Bsu15I ATCGAT 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 96
BtgI CCRYGG 1 cut(s) 9
BtrI CACGTC 1 cut(s) 131
BtsCI GGATG 1 cut(s) 141
BtsIMutI CAGTG 3 cut(s) 175, 627, 786
Cac8I GCNNGC 1 cut(s) 691
CfoI GCGC 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 96
CviAII CATG 3 cut(s) 64, 192, 214
CviJI RGCY 7 cut(s) 23, 325, 333, 411, 447, 477, 648
CviKI_1 RGCY 7 cut(s) 23, 325, 333, 411, 447, 477, 648
DdeI CTNAG 4 cut(s) 148, 295, 336, 662
DraIII CACNNNGTG 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 413
Eco31I GGTCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 74
Eco88I CYCGRG 1 cut(s) 747
EcoT38I GRGCYC 1 cut(s) 413
FaeI CATG 3 cut(s) 67, 195, 217
FalI AAGNNNNNCTT 2 cut(s) 538, 570
FaqI GGGAC 2 cut(s) 410, 494
FatI CATG 3 cut(s) 63, 191, 213
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 413
Fsp4HI GCNGC 1 cut(s) 523
FspBI CTAG 4 cut(s) 141, 405, 414, 534
GlaI GCGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 523
GsuI CTGGAG 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 148
HapII CCGG 4 cut(s) 343, 478, 564, 706
HhaI GCGC 1 cut(s) 147
Hin1II CATG 3 cut(s) 67, 195, 217
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 323
HinfI GANTC 3 cut(s) 398, 697, 869
HpaII CCGG 4 cut(s) 343, 478, 564, 706
Hpy166II GTNNAC 5 cut(s) 46, 128, 173, 569, 838
Hpy188I TCNGA 4 cut(s) 93, 151, 337, 874
Hpy8I GTNNAC 5 cut(s) 46, 128, 173, 569, 838
Hpy99I CGWCG 1 cut(s) 222
HpyAV CCTTC 2 cut(s) 192, 636
HpyCH4III ACNGT 2 cut(s) 452, 738
HpyCH4IV ACGT 2 cut(s) 130, 840
HpyCH4V TGCA 4 cut(s) 4, 61, 853, 879
HpyF10VI GCNNNNNNNGC 2 cut(s) 417, 453
HpyF3I CTNAG 4 cut(s) 148, 295, 336, 662
HpySE526I ACGT 2 cut(s) 130, 840
Hsp92II CATG 3 cut(s) 67, 195, 217
HspAI GCGC 1 cut(s) 145
Kpn2I TCCGGA 2 cut(s) 342, 705
LmnI GCTCC 6 cut(s) 408, 444, 492, 708, 714, 720
LweI GCATC 2 cut(s) 48, 376
MaeI CTAG 4 cut(s) 141, 405, 414, 534
MaeII ACGT 2 cut(s) 130, 840
MaeIII GTNAC 2 cut(s) 104, 732
MbiI CCGCTC 1 cut(s) 525
MboII GAAGA 7 cut(s) 77, 80, 311, 403, 507, 664, 791
MflI RGATCY 3 cut(s) 199, 536, 548
MhlI GDGCHC 1 cut(s) 413
MluCI AATT 3 cut(s) 820, 857, 880
MlyI GAGTC 3 cut(s) 392, 706, 863
MnlI CCTC 5 cut(s) 61, 116, 369, 387, 826
MroI TCCGGA 2 cut(s) 342, 705
MseI TTAA 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 203
MspI CCGG 4 cut(s) 343, 478, 564, 706
MspR9I CCNGG 2 cut(s) 479, 564
Mva1269I GAATGC 2 cut(s) 78, 352
MvnI CGCG 1 cut(s) 420
MwoI GCNNNNNNNGC 2 cut(s) 417, 453
NciI CCSGG 2 cut(s) 479, 564
NlaIII CATG 3 cut(s) 67, 195, 217
NlaIV GGNNCC 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 732
NspI RCATGY 1 cut(s) 217
PaeR7I CTCGAG 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PctI GAATGC 2 cut(s) 78, 352
PkrI GCNGC 1 cut(s) 524
PleI GAGTC 3 cut(s) 392, 705, 863
PpsI GAGTC 3 cut(s) 392, 705, 863
Psp1406I AACGTT 1 cut(s) 840
PspN4I GGNNCC 1 cut(s) 410
PsuI RGATCY 3 cut(s) 199, 536, 548
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 1 cut(s) 523
SchI GAGTC 3 cut(s) 392, 706, 863
ScrFI CCNGG 2 cut(s) 479, 564
SduI GDGCHC 1 cut(s) 413
SetI ASST 7 cut(s) 127, 133, 327, 449, 581, 663, 843
SfaNI GCATC 2 cut(s) 48, 376
SfcI CTRYAG 2 cut(s) 109, 448
Sfr274I CTCGAG 1 cut(s) 747
SlaI CTCGAG 1 cut(s) 747
SmlI CTYRAG 2 cut(s) 203, 747
SmoI CTYRAG 2 cut(s) 203, 747
Sse9I AATT 3 cut(s) 820, 857, 880
SsiI CCGC 4 cut(s) 186, 358, 420, 523
SspMI CTAG 4 cut(s) 141, 405, 414, 534
StyD4I CCNGG 2 cut(s) 477, 562
TaaI ACNGT 2 cut(s) 452, 738
TaiI ACGT 2 cut(s) 133, 843
TaqI TCGA 7 cut(s) 96, 121, 424, 541, 748, 804, 818
TasI AATT 3 cut(s) 820, 857, 880
TauI GCSGC 1 cut(s) 525
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 3 cut(s) 175, 627, 786
TseFI GTSAC 1 cut(s) 732
Tsp45I GTSAC 1 cut(s) 732
TspDTI ATGAA 3 cut(s) 71, 180, 320
TspRI CASTG 3 cut(s) 175, 627, 786
Vha464I CTTAAG 1 cut(s) 203
XceI RCATGY 1 cut(s) 217
XhoI CTCGAG 1 cut(s) 747
XspI CTAG 4 cut(s) 141, 405, 414, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.