pycom12652g00020

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012652
N/A
Physical Location & Seq
Reverse (-)
26804 .. 27762
959 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 537 bp
ATGTTGAGATTTCTTGCCTCTGTTATTGTATTTTCTTTCAGCTCCTGTGTTAGGGGTGATTTGCATGCCCTGGAAGATTTGACTTATGAGAAAAGAAGAGAATTTTTGAGGAAACACCCATTGCCAAAGGAACTCTCCGTTGCTTCATTCCACACAGAAGCTGCCATTTCTCCTGCTGTTTTAGCTACATTGTCTCGTGTAGCGCATGCCGAGCTACCTGCCGCTCTTTCTGCAGGCCAAGCAGCAAAACTTCCAGTGGTAATGCCCCTTGGTGCAGCTATGGCTGCCTGTGCGCAGCTTCTGCAGGTTAGATATGGCAAGAAGAGTGATGGACTTGTCACATGCTGCAACGCAGAAGTTCCTGGATCTATTGTTGTGCGGCCGAAGCGTAAATTAGACCATGGCTGGATGGTTTATTCGTCGTTGTCAGACAACCCTTGTGAAGCAGATGCTTCTCAAGTTTGTGAAGCTCTTTTGACATTGCTTGTCAAAGCTGGGCAGAACAAGAGACACGATAATGGGACGAAAGATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LCAT

Protein Analysis

179

Amino Acids

19.21

Weight (kDa)

8.55

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 294
Acc36I ACCTGC 2 cut(s) 226, 295
AccBSI CCGCTC 1 cut(s) 224
AciI CCGC 2 cut(s) 222, 379
AclWI GGATC 1 cut(s) 373
AcoI YGGCCR 1 cut(s) 380
AcsI RAATTY 1 cut(s) 101
AfiI CCNNNNNNNGG 1 cut(s) 51
AjnI CCWGG 2 cut(s) 69, 361
AluBI AGCT 8 cut(s) 42, 161, 185, 214, 278, 298, 470, 494
AluI AGCT 8 cut(s) 42, 161, 185, 214, 278, 298, 470, 494
Alw26I GTCTC 2 cut(s) 198, 502
AlwI GGATC 1 cut(s) 373
AlwNI CAGNNNCTG 3 cut(s) 45, 161, 301
AoxI GGCC 2 cut(s) 235, 380
ApeKI GCWGC 6 cut(s) 161, 242, 275, 284, 295, 345
ApoI RAATTY 1 cut(s) 101
AspLEI GCGC 2 cut(s) 205, 295
AsuHPI GGTGA 1 cut(s) 68
BauI CACGAG 1 cut(s) 195
BbvI GCAGC 6 cut(s) 148, 254, 271, 287, 307, 332
BccI CCATC 2 cut(s) 323, 403
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BciT130I CCWGG 2 cut(s) 71, 363
BcoDI GTCTC 2 cut(s) 198, 502
BfmI CTRYAG 2 cut(s) 231, 302
BfuAI ACCTGC 2 cut(s) 226, 295
BisI GCNGC 8 cut(s) 162, 222, 243, 276, 285, 296, 346, 380
BlsI GCNGC 8 cut(s) 163, 223, 244, 277, 286, 297, 347, 381
Bme1390I CCNGG 2 cut(s) 71, 363
BmrFI CCNGG 2 cut(s) 71, 363
BmsI GCATC 1 cut(s) 439
BpuEI CTTGAG 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 69, 268, 400
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 2 cut(s) 119, 479
BseBI CCWGG 2 cut(s) 71, 363
BseDI CCNNGG 3 cut(s) 69, 268, 400
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMI GCAATG 2 cut(s) 119, 479
BseNI ACTGG 1 cut(s) 254
BseX3I CGGCCG 1 cut(s) 380
BseXI GCAGC 6 cut(s) 148, 254, 271, 287, 307, 332
BseYI CCCAGC 1 cut(s) 494
BsgI GTGCAG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 383
BshFI GGCC 2 cut(s) 237, 382
BsiEI CGRYCG 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 2 cut(s) 198, 502
BsnI GGCC 2 cut(s) 237, 382
Bsp143I GATC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 400
BspACI CCGC 2 cut(s) 222, 379
BspANI GGCC 2 cut(s) 237, 382
BspMAI CTGCAG 2 cut(s) 235, 306
BspMI ACCTGC 2 cut(s) 226, 295
BspPI GGATC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 2 cut(s) 119, 479
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 3 cut(s) 69, 268, 400
BssMI GATC 1 cut(s) 365
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 268, 400
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 71, 363
Bst6I CTCTTC 2 cut(s) 91, 317
BstAPI GCANNNNNTGC 1 cut(s) 301
BstC8I GCNNGC 3 cut(s) 66, 207, 235
BstDSI CCRYGG 1 cut(s) 400
BstENI CCTNNNNNAGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 414
BstHHI GCGC 2 cut(s) 205, 295
BstKTI GATC 1 cut(s) 368
BstMAI GTCTC 2 cut(s) 198, 502
BstMBI GATC 1 cut(s) 365
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 9 cut(s) 182, 211, 230, 239, 281, 284, 290, 301, 385
BstNI CCWGG 2 cut(s) 71, 363
BstNSI RCATGY 3 cut(s) 68, 209, 345
BstSCI CCNGG 2 cut(s) 69, 361
BstSFI CTRYAG 2 cut(s) 231, 302
BstV1I GCAGC 6 cut(s) 148, 254, 271, 287, 307, 332
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
BstZI CGGCCG 1 cut(s) 380
BsuRI GGCC 2 cut(s) 237, 382
BtgI CCRYGG 1 cut(s) 400
BtsCI GGATG 1 cut(s) 414
BtsIMutI CAGTG 1 cut(s) 261
BveI ACCTGC 2 cut(s) 226, 295
Cac8I GCNNGC 3 cut(s) 66, 207, 235
CaiI CAGNNNCTG 3 cut(s) 45, 161, 301
CfoI GCGC 2 cut(s) 205, 295
CviAII CATG 4 cut(s) 65, 206, 342, 401
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 380
EagI CGGCCG 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 91, 317
EarI CTCTTC 2 cut(s) 91, 317
EclXI CGGCCG 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 268, 400
Eco52I CGGCCG 1 cut(s) 380
EcoNI CCTNNNNNAGG 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 69, 361
EcoT14I CCWWGG 2 cut(s) 268, 400
ErhI CCWWGG 2 cut(s) 268, 400
FaeI CATG 4 cut(s) 68, 209, 345, 404
FaiI YATR 7 cut(s) 66, 87, 207, 281, 315, 343, 402
FatI CATG 4 cut(s) 64, 205, 341, 400
Fnu4HI GCNGC 8 cut(s) 162, 222, 243, 276, 285, 296, 346, 380
FokI GGATG 1 cut(s) 421
Fsp4HI GCNGC 8 cut(s) 162, 222, 243, 276, 285, 296, 346, 380
FspI TGCGCA 1 cut(s) 294
GlaI GCGC 2 cut(s) 204, 294
GluI GCNGC 8 cut(s) 162, 222, 243, 276, 285, 296, 346, 380
GsaI CCCAGC 1 cut(s) 498
HaeIII GGCC 2 cut(s) 237, 382
HhaI GCGC 2 cut(s) 205, 295
Hin1II CATG 4 cut(s) 68, 209, 345, 404
Hin6I GCGC 2 cut(s) 203, 293
HinP1I GCGC 2 cut(s) 203, 293
HphI GGTGA 1 cut(s) 68
Hpy188I TCNGA 1 cut(s) 430
Hpy99I CGWCG 1 cut(s) 424
HpyCH4V TGCA 5 cut(s) 64, 233, 275, 304, 348
HpyF10VI GCNNNNNNNGC 9 cut(s) 182, 211, 230, 239, 281, 284, 290, 301, 385
Hsp92II CATG 4 cut(s) 68, 209, 345, 404
HspAI GCGC 2 cut(s) 203, 293
Kzo9I GATC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 47
Lsp1109I GCAGC 6 cut(s) 148, 254, 271, 287, 307, 332
LweI GCATC 1 cut(s) 439
MaeIII GTNAC 1 cut(s) 337
MalI GATC 1 cut(s) 367
MbiI CCGCTC 1 cut(s) 224
MboI GATC 1 cut(s) 365
MboII GAAGA 3 cut(s) 86, 108, 334
MflI RGATCY 1 cut(s) 365
MluCI AATT 2 cut(s) 101, 392
MnlI CCTC 2 cut(s) 28, 102
MslI CAYNNNNRTG 1 cut(s) 516
MspR9I CCNGG 2 cut(s) 71, 363
MvaI CCWGG 2 cut(s) 71, 363
MwoI GCNNNNNNNGC 9 cut(s) 182, 211, 230, 239, 281, 284, 290, 301, 385
NcoI CCATGG 1 cut(s) 400
NdeII GATC 1 cut(s) 365
NlaIII CATG 4 cut(s) 68, 209, 345, 404
NmeAIII GCCGAG 1 cut(s) 235
NmuCI GTSAC 1 cut(s) 337
NsbI TGCGCA 1 cut(s) 294
NspI RCATGY 3 cut(s) 68, 209, 345
PaeI GCATGC 2 cut(s) 68, 209
PfoI TCCNGGA 1 cut(s) 361
PkrI GCNGC 8 cut(s) 163, 223, 244, 277, 286, 297, 347, 381
Psp6I CCWGG 2 cut(s) 69, 361
PspFI CCCAGC 1 cut(s) 494
PspGI CCWGG 2 cut(s) 69, 361
PstI CTGCAG 2 cut(s) 235, 306
PstNI CAGNNNCTG 3 cut(s) 45, 161, 301
PsuI RGATCY 1 cut(s) 365
RseI CAYNNNNRTG 1 cut(s) 516
SatI GCNGC 8 cut(s) 162, 222, 243, 276, 285, 296, 346, 380
Sau3AI GATC 1 cut(s) 365
ScrFI CCNGG 2 cut(s) 71, 363
SfaNI GCATC 1 cut(s) 439
SfcI CTRYAG 2 cut(s) 231, 302
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 456
SmoI CTYRAG 1 cut(s) 456
SphI GCATGC 2 cut(s) 68, 209
Sse9I AATT 2 cut(s) 101, 392
SsiI CCGC 2 cut(s) 222, 379
StyD4I CCNGG 2 cut(s) 69, 361
StyI CCWWGG 2 cut(s) 268, 400
TasI AATT 2 cut(s) 101, 392
TauI GCSGC 2 cut(s) 224, 382
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 6 cut(s) 161, 242, 275, 284, 295, 345
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 1 cut(s) 135
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 1 cut(s) 261
XagI CCTNNNNNAGG 1 cut(s) 49
XapI RAATTY 1 cut(s) 101
XceI RCATGY 3 cut(s) 68, 209, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.