pycom12g01180

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
991698 .. 994075
2378 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 288 bp
ATGGCCAATGTCCTCCGAACTTCACCTTTCTCGGCGCCAATTGCACCGCCGCCCTCCGCCGCTTCGCCCAACTCTAGAAAACCGTCGTACGTTTCGTTTCGGCGAAGTCAAACCGGCAGCAGTAGCACGCCTTATCTCACTATAAGCTCCCTCCGGTTCTCTTACTTGCCGTCCCTCCGGCTCGTCAAGTTCGCGCCTTTGGCCTCACAAGGTGAGACCGAGACGGCTGGGACGGTGGAGGAGGAGAGAACTAGACTCTTTGGATGGTGCAATTCCTGTGGAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GrpE

Protein Analysis

96

Amino Acids

10.32

Weight (kDa)

10.17

Isoelectric Point (pI)

79.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 1 cut(s) 194
AciI CCGC 4 cut(s) 47, 50, 57, 60
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 212
AfaI GTAC 1 cut(s) 89
AluBI AGCT 1 cut(s) 147
AluI AGCT 1 cut(s) 147
Alw26I GTCTC 2 cut(s) 209, 215
AoxI GGCC 2 cut(s) 3, 201
ApeKI GCWGC 1 cut(s) 117
AspLEI GCGC 2 cut(s) 37, 196
AsuHPI GGTGA 2 cut(s) 15, 224
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 34
BbvI GCAGC 1 cut(s) 129
BccI CCATC 1 cut(s) 258
BceAI ACGGC 2 cut(s) 154, 240
BcoDI GTCTC 2 cut(s) 209, 215
BfaI CTAG 2 cut(s) 75, 252
BfoI RGCGCY 1 cut(s) 38
BisI GCNGC 3 cut(s) 50, 60, 118
BlsI GCNGC 3 cut(s) 51, 61, 119
BmiI GGNNCC 1 cut(s) 36
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 209
BsaWI WCCGGW 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 113
BseGI GGATG 1 cut(s) 269
BseRI GAGGAG 2 cut(s) 254, 257
BseXI GCAGC 1 cut(s) 129
BseYI CCCAGC 1 cut(s) 227
Bsh1236I CGCG 1 cut(s) 194
BshFI GGCC 2 cut(s) 5, 203
BshNI GGYRCC 1 cut(s) 34
BsiSI CCGG 3 cut(s) 114, 154, 178
BsiWI CGTACG 1 cut(s) 87
BslFI GGGAC 2 cut(s) 157, 244
BsmAI GTCTC 2 cut(s) 209, 215
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 157, 244
BsnI GGCC 2 cut(s) 5, 203
Bso31I GGTCTC 1 cut(s) 209
BspACI CCGC 4 cut(s) 47, 50, 57, 60
BspANI GGCC 2 cut(s) 5, 203
BspFNI CGCG 1 cut(s) 194
BspLI GGNNCC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 209
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssNI GRCGYC 1 cut(s) 35
Bst4CI ACNGT 2 cut(s) 84, 235
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 128
BstF5I GGATG 1 cut(s) 269
BstFNI CGCG 1 cut(s) 194
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 2 cut(s) 37, 196
BstMAI GTCTC 2 cut(s) 209, 215
BstMWI GCNNNNNNNGC 3 cut(s) 41, 123, 200
BstUI CGCG 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 5, 203
BtsCI GGATG 1 cut(s) 269
Cac8I GCNNGC 1 cut(s) 128
CfoI GCGC 2 cut(s) 37, 196
Cfr10I RCCGGY 1 cut(s) 113
Csp6I GTAC 1 cut(s) 88
CspCI CAANNNNNGTGG 1 cut(s) 259
CviJI RGCY 5 cut(s) 5, 147, 181, 203, 227
CviKI_1 RGCY 5 cut(s) 5, 147, 181, 203, 227
CviQI GTAC 1 cut(s) 88
DinI GGCGCC 1 cut(s) 36
DraIII CACNNNGTG 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 209
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
Esp3I CGTCTC 1 cut(s) 215
FaiI YATR 1 cut(s) 143
FaqI GGGAC 2 cut(s) 157, 244
Fnu4HI GCNGC 3 cut(s) 50, 60, 118
FokI GGATG 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 50, 60, 118
FspBI CTAG 2 cut(s) 75, 252
GlaI GCGC 2 cut(s) 36, 195
GluI GCNGC 3 cut(s) 50, 60, 118
GsaI CCCAGC 1 cut(s) 231
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 2 cut(s) 5, 203
HapII CCGG 3 cut(s) 114, 154, 178
HhaI GCGC 2 cut(s) 37, 196
Hin1I GRCGYC 1 cut(s) 35
Hin6I GCGC 2 cut(s) 35, 194
HinP1I GCGC 2 cut(s) 35, 194
HinfI GANTC 1 cut(s) 255
HpaII CCGG 3 cut(s) 114, 154, 178
HphI GGTGA 2 cut(s) 15, 224
Hpy188I TCNGA 1 cut(s) 17
Hpy188III TCNNGA 1 cut(s) 75
Hpy99I CGWCG 1 cut(s) 88
HpyCH4III ACNGT 2 cut(s) 84, 235
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 2 cut(s) 44, 270
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 123, 200
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 35
HspAI GCGC 2 cut(s) 35, 194
KasI GGCGCC 1 cut(s) 34
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 4 cut(s) 127, 167, 191, 213
Lsp1109I GCAGC 1 cut(s) 129
MaeI CTAG 2 cut(s) 75, 252
MaeII ACGT 1 cut(s) 90
MfeI CAATTG 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 39, 271
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 35
MlyI GAGTC 1 cut(s) 249
MnlI CCTC 7 cut(s) 23, 64, 161, 185, 214, 232, 235
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 114, 154, 178
MunI CAATTG 1 cut(s) 39
MvnI CGCG 1 cut(s) 194
MwoI GCNNNNNNNGC 3 cut(s) 41, 123, 200
NarI GGCGCC 1 cut(s) 35
NlaIV GGNNCC 1 cut(s) 36
NmeAIII GCCGAG 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 92
Pfl23II CGTACG 1 cut(s) 87
PkrI GCNGC 3 cut(s) 51, 61, 119
PleI GAGTC 1 cut(s) 249
PluTI GGCGCC 1 cut(s) 38
PpsI GAGTC 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 227
PspLI CGTACG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 36
RsaI GTAC 1 cut(s) 89
RsaNI GTAC 1 cut(s) 88
SatI GCNGC 3 cut(s) 50, 60, 118
SchI GAGTC 1 cut(s) 249
SetI ASST 4 cut(s) 28, 93, 149, 214
SfoI GGCGCC 1 cut(s) 36
Sse9I AATT 2 cut(s) 39, 271
SsiI CCGC 4 cut(s) 47, 50, 57, 60
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 2 cut(s) 75, 252
TaaI ACNGT 2 cut(s) 84, 235
TaiI ACGT 1 cut(s) 93
TaqII GACCGA 1 cut(s) 233
TasI AATT 2 cut(s) 39, 271
TauI GCSGC 2 cut(s) 52, 62
TseI GCWGC 1 cut(s) 117
XbaI TCTAGA 1 cut(s) 74
XspI CTAG 2 cut(s) 75, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.