pycom12g01440

Permuted single zf-CXXC unit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
1197786 .. 1198436
651 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGCTGCATTACAGTCTCAGGAGCACCCGTATGTCCTCTCATGACGTATCAAACTGTGGAGGTTACCAACCTGCATGTGTGCAGCATAATGACCAAAGATGTGAGATAAACAGAGAAGCATCCCTTGAGCCTTCCAGTAACTGTCGTTTGAATCTAACCCCCAGCCCAGAATTACAGCGAGTTGAGTACTTTGATTTGTACGGAGAAGTAACCCAGTCTTCTTATGCATCCAAAAATAAATGTGAAGATAGTCCGAGTGAAAGAAGTGCACTAACAGCAGAATCTCCCAGTCAAGATACAATACATAATAAGCTAACAGCGAATGTTCAAGAAGCACCAAGATGTTCCGGAAATTCATGCAATAACATTCAGGTAGGCAATAATATGGCCCAGTCTCAACTCGGGTTGGCTGGGAGCTCAAATAATGTTGACATACATTCCCAGAAGCAAAACAATAAGATACAGCAAAGTTGCTTGAACATTTCTGGAGGGACCACAGATGTTATGCAGAAGGCAGCAGAGTTGGGTTCAAATGAGCAGAGTAATTCAGTTAGCAAGGAGTTCAGTAGTACAAATGCTGCTACCTCCAAAACAAAAAACAGAAAAGCTGGAAAAGAGGAAAACGATCGATCAATGATTGGGACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003906 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006281 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0016043 GO:0016458 GO:0016787 GO:0016798 GO:0016799 GO:0019104 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031935 GO:0031936 GO:0032259 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0035510 GO:0040029 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045815 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0060968 GO:0060969 GO:0065007 GO:0070988 GO:0071704 GO:0071840 GO:0080090 GO:0080111 GO:0090304 GO:0098542 GO:0140097 GO:1901360 GO:1902275 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1905268 GO:2000112 GO:2000113 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

217

Amino Acids

23.51

Weight (kDa)

6.21

Isoelectric Point (pI)

72.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
AccIII TCCGGA 1 cut(s) 347
AcsI RAATTY 1 cut(s) 352
AfaI GTAC 3 cut(s) 188, 200, 571
AgsI TTSAA 4 cut(s) 151, 329, 478, 531
AluBI AGCT 3 cut(s) 313, 417, 608
AluI AGCT 3 cut(s) 313, 417, 608
Alw21I GWGCWC 3 cut(s) 26, 271, 419
Alw26I GTCTC 2 cut(s) 20, 399
Alw44I GTGCAC 1 cut(s) 267
AlwNI CAGNNNCTG 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 401
Aor13HI TCCGGA 1 cut(s) 347
AoxI GGCC 1 cut(s) 387
ApaLI GTGCAC 1 cut(s) 267
ApeKI GCWGC 4 cut(s) 4, 82, 515, 578
ApoI RAATTY 1 cut(s) 352
AspS9I GGNCC 2 cut(s) 388, 492
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 271
BanII GRGCYC 1 cut(s) 419
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 3 cut(s) 26, 271, 419
BbvI GCAGC 3 cut(s) 94, 527, 565
BcoDI GTCTC 2 cut(s) 20, 399
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 4 cut(s) 5, 83, 516, 579
BlsI GCNGC 4 cut(s) 6, 84, 517, 580
BmcAI AGTACT 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 388, 492
BmiI GGNNCC 1 cut(s) 493
BmrI ACTGGG 3 cut(s) 208, 282, 385
BmsI GCATC 2 cut(s) 128, 236
BmuI ACTGGG 3 cut(s) 208, 282, 385
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 628
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bse1I ACTGG 4 cut(s) 135, 214, 288, 391
BseAI TCCGGA 1 cut(s) 347
BseCI ATCGAT 1 cut(s) 628
BseGI GGATG 2 cut(s) 119, 227
BseMII CTCAG 1 cut(s) 31
BseNI ACTGG 4 cut(s) 135, 214, 288, 391
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 3 cut(s) 94, 527, 565
BseYI CCCAGC 2 cut(s) 161, 410
BsgI GTGCAG 1 cut(s) 101
Bsh1285I CGRYCG 1 cut(s) 628
BshFI GGCC 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 628
BsiEI CGRYCG 1 cut(s) 628
BsiHKAI GWGCWC 3 cut(s) 26, 271, 419
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 1 cut(s) 505
BsmAI GTCTC 2 cut(s) 20, 399
BsmFI GGGAC 1 cut(s) 505
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 26, 271, 419
Bsp13I TCCGGA 1 cut(s) 347
Bsp143I GATC 2 cut(s) 625, 629
BspANI GGCC 1 cut(s) 389
BspCNI CTCAG 1 cut(s) 30
BspDI ATCGAT 1 cut(s) 628
BspEI TCCGGA 1 cut(s) 347
BspHI TCATGA 1 cut(s) 40
BspLI GGNNCC 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 79
BsrI ACTGG 4 cut(s) 135, 214, 288, 391
BssMI GATC 2 cut(s) 625, 629
Bst4CI ACNGT 3 cut(s) 14, 56, 143
BstDEI CTNAG 1 cut(s) 17
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 119, 227
BstKTI GATC 2 cut(s) 628, 632
BstMAI GTCTC 2 cut(s) 20, 399
BstMBI GATC 2 cut(s) 625, 629
BstMCI CGRYCG 1 cut(s) 628
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNSI RCATGY 1 cut(s) 78
BstPI GGTNACC 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 94, 527, 565
BstV2I GAAGAC 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 628
BsuRI GGCC 1 cut(s) 389
BsuTUI ATCGAT 1 cut(s) 628
BtsCI GGATG 2 cut(s) 119, 227
BveI ACCTGC 1 cut(s) 79
CaiI CAGNNNCTG 1 cut(s) 141
CciI TCATGA 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 388, 492
ClaI ATCGAT 1 cut(s) 628
Csp6I GTAC 3 cut(s) 187, 199, 570
CviAII CATG 3 cut(s) 41, 75, 357
CviJI RGCY 7 cut(s) 130, 165, 313, 389, 410, 417, 608
CviKI_1 RGCY 7 cut(s) 130, 165, 313, 389, 410, 417, 608
CviQI GTAC 3 cut(s) 187, 199, 570
DdeI CTNAG 1 cut(s) 17
DpnI GATC 2 cut(s) 627, 631
DpnII GATC 2 cut(s) 625, 629
Ecl136II GAGCTC 1 cut(s) 417
Eco24I GRGCYC 1 cut(s) 419
Eco47I GGWCC 1 cut(s) 492
Eco53kI GAGCTC 1 cut(s) 417
Eco88I CYCGRG 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 417
EcoO65I GGTNACC 1 cut(s) 62
EcoT22I ATGCAT 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 419
FaeI CATG 3 cut(s) 44, 78, 360
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 1 cut(s) 505
FatI CATG 3 cut(s) 40, 74, 356
Fnu4HI GCNGC 4 cut(s) 5, 83, 516, 579
FokI GGATG 2 cut(s) 106, 214
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 4 cut(s) 5, 83, 516, 579
GluI GCNGC 4 cut(s) 5, 83, 516, 579
GsaI CCCAGC 2 cut(s) 165, 414
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 389
HapII CCGG 1 cut(s) 348
Hin1II CATG 3 cut(s) 44, 78, 360
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 2 cut(s) 151, 281
HpaII CCGG 1 cut(s) 348
Hpy166II GTNNAC 2 cut(s) 269, 430
Hpy188I TCNGA 1 cut(s) 255
Hpy188III TCNNGA 6 cut(s) 19, 41, 293, 329, 348, 486
Hpy8I GTNNAC 2 cut(s) 269, 430
HpyAV CCTTC 2 cut(s) 141, 505
HpyCH4III ACNGT 3 cut(s) 14, 56, 143
HpyCH4IV ACGT 1 cut(s) 45
HpyCH4V TGCA 7 cut(s) 7, 74, 82, 227, 269, 360, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 17
HpySE526I ACGT 1 cut(s) 45
Hsp92II CATG 3 cut(s) 44, 78, 360
Kpn2I TCCGGA 1 cut(s) 347
Kzo9I GATC 2 cut(s) 625, 629
LmnI GCTCC 2 cut(s) 21, 414
Lsp1109I GCAGC 3 cut(s) 94, 527, 565
LweI GCATC 2 cut(s) 128, 236
MaeII ACGT 1 cut(s) 45
MaeIII GTNAC 3 cut(s) 62, 137, 208
MalI GATC 2 cut(s) 627, 631
MboI GATC 2 cut(s) 625, 629
MboII GAAGA 2 cut(s) 210, 257
MhlI GDGCHC 3 cut(s) 26, 271, 419
MluCI AATT 4 cut(s) 170, 352, 544, 646
MnlI CCTC 5 cut(s) 46, 53, 482, 595, 610
Mph1103I ATGCAT 1 cut(s) 229
MroI TCCGGA 1 cut(s) 347
MseI TTAA 1 cut(s) 649
MslI CAYNNNNRTG 2 cut(s) 29, 340
MspI CCGG 1 cut(s) 348
MwoI GCNNNNNNNGC 1 cut(s) 275
NdeII GATC 2 cut(s) 625, 629
NlaIII CATG 3 cut(s) 44, 78, 360
NlaIV GGNNCC 1 cut(s) 493
NsiI ATGCAT 1 cut(s) 229
NspI RCATGY 1 cut(s) 78
PagI TCATGA 1 cut(s) 40
PfeI GAWTC 2 cut(s) 151, 281
PkrI GCNGC 4 cut(s) 6, 84, 517, 580
Ple19I CGATCG 1 cut(s) 628
Psp124BI GAGCTC 1 cut(s) 419
PspEI GGTNACC 1 cut(s) 62
PspFI CCCAGC 2 cut(s) 161, 410
PspN4I GGNNCC 1 cut(s) 493
PspPI GGNCC 2 cut(s) 388, 492
PstNI CAGNNNCTG 1 cut(s) 141
PvuI CGATCG 1 cut(s) 628
RsaI GTAC 3 cut(s) 188, 200, 571
RsaNI GTAC 3 cut(s) 187, 199, 570
RseI CAYNNNNRTG 2 cut(s) 29, 340
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 1 cut(s) 649
SatI GCNGC 4 cut(s) 5, 83, 516, 579
Sau3AI GATC 2 cut(s) 625, 629
Sau96I GGNCC 2 cut(s) 388, 492
ScaI AGTACT 1 cut(s) 188
SduI GDGCHC 3 cut(s) 26, 271, 419
SetI ASST 8 cut(s) 48, 64, 73, 315, 375, 419, 587, 610
SfaNI GCATC 2 cut(s) 128, 236
SinI GGWCC 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 29, 340
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
Sse9I AATT 4 cut(s) 170, 352, 544, 646
SstI GAGCTC 1 cut(s) 419
TaaI ACNGT 3 cut(s) 14, 56, 143
TaiI ACGT 1 cut(s) 48
TaqI TCGA 1 cut(s) 628
TasI AATT 4 cut(s) 170, 352, 544, 646
TatI WGTACW 2 cut(s) 186, 569
TfiI GAWTC 2 cut(s) 151, 281
Tru1I TTAA 1 cut(s) 649
Tru9I TTAA 1 cut(s) 649
TseI GCWGC 4 cut(s) 4, 82, 515, 578
TspDTI ATGAA 1 cut(s) 345
TspGWI ACGGA 1 cut(s) 216
VneI GTGCAC 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 492
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 188
Zsp2I ATGCAT 1 cut(s) 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.