pycom12g02710

1-aminocyclopropane-1-carboxylate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
2450315 .. 2451429
1115 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 825 bp
ATGCACAAGTCTATCAATTCTACTCACAAAATGGCAGCCAAAATTGATGCAACATCGGGATCATACGACCGCATGAAAGAAGTGAAAGAATTCGATGATTCGAAGATGGGAGTCAAAGGCCTCTCCGATTCAGGCATCACTGTCATCCCTCAAATTTTTGTCCACGACCCTCAAACCTTATCAGATCTCAAACCCTCATCCAAAAACACCACCACCACCATTCCCACCATCGACCTTTCTCACATCAACTCAGCCGCCCACCGCCCCAAAATCGTTGACCAAGTGAAATCCGTCGCCAATACCTTTGGCTTCTTCCAAGTCACCAACCATGGGGTGCCGATCTCCGTTTTGGATGAAACGATCACCGCTGTCAAAGCGTTCCACGAGCTGCCGCAGGAGGACAGAGCCAAGTACTACAGGCGAAATGAAGGAAAGGGAGTCATGTACGCTAGCAATGCCGACTTGTACTGCACATCAGCCGCCAGCTGGCGTGATTCTCTACACGTGTGGATGAGCCCAGAGAGGGCCAAGGCCGAGGAGGTACCTGAGGCGTGTCGGGAAGAGTTGTCTGCTTGGGATCTGCATGCAACCAAGGTGGCGAATGACGTGTTTGAGTTGCTTTCGGAAGGGTTAGGGTTGGAGGGAAAGAGATTTAGGGGTCATGTCTCTTTCTCATTCCTGGTCAAACTCCTTATTGATTCCAATCTCTATTTCTCTGTCCTCCCTCATTCTCTAGCAATGCGTTGTTCGAATGGAACCTCACGGCTCCAATTTTCCCGACGAGTTGCAGGTTTCCCGATGTGTTTTCCGGCCAACCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

275

Amino Acids

30.52

Weight (kDa)

8.29

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 74 - 176 5.5e-19 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 779
Acc65I GGTACC 1 cut(s) 541
AccB1I GGYRCC 2 cut(s) 334, 541
AciI CCGC 6 cut(s) 70, 255, 262, 366, 392, 480
AclWI GGATC 2 cut(s) 67, 585
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 2 cut(s) 89, 153
AcvI CACGTG 1 cut(s) 505
AfaI GTAC 4 cut(s) 413, 446, 467, 543
AfiI CCNNNNNNNGG 3 cut(s) 330, 486, 523
AflIII ACRYGT 3 cut(s) 502, 504, 606
AjiI CACGTC 1 cut(s) 607
AjnI CCWGG 1 cut(s) 678
AjuI GAANNNNNNNTTGG 2 cut(s) 695, 727
AluBI AGCT 2 cut(s) 388, 486
AluI AGCT 2 cut(s) 388, 486
Alw26I GTCTC 1 cut(s) 670
AlwI GGATC 2 cut(s) 67, 585
AoxI GGCC 4 cut(s) 118, 525, 531, 810
ApeKI GCWGC 2 cut(s) 35, 388
ApoI RAATTY 2 cut(s) 89, 153
Asp700I GAANNNNTTC 1 cut(s) 89
Asp718I GGTACC 1 cut(s) 541
AspS9I GGNCC 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 313, 355
AsuII TTCGAA 2 cut(s) 101, 749
AsuNHI GCTAGC 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 546
BanI GGYRCC 2 cut(s) 334, 541
BanII GRGCYC 1 cut(s) 518
BauI CACGAG 1 cut(s) 383
BbrPI CACGTG 1 cut(s) 505
BbvI GCAGC 2 cut(s) 47, 375
BccI CCATC 2 cut(s) 100, 236
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 1 cut(s) 680
BcoDI GTCTC 1 cut(s) 670
BfaI CTAG 2 cut(s) 450, 734
BfmI CTRYAG 1 cut(s) 415
BfuAI ACCTGC 1 cut(s) 779
BglII AGATCT 1 cut(s) 184
BisI GCNGC 5 cut(s) 36, 255, 389, 392, 480
BlsI GCNGC 5 cut(s) 37, 256, 390, 393, 481
BmcAI AGTACT 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 680
BmgBI CACGTC 1 cut(s) 607
BmgT120I GGNCC 1 cut(s) 525
BmiI GGNNCC 4 cut(s) 336, 543, 757, 767
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 2 cut(s) 37, 144
BmtI GCTAGC 1 cut(s) 453
Bpu14I TTCGAA 2 cut(s) 101, 749
BsaAI YACGTR 1 cut(s) 505
BsaBI GATNNNNATC 1 cut(s) 702
BsaJI CCNNGG 4 cut(s) 328, 528, 534, 591
Bsc4I CCNNNNNNNGG 3 cut(s) 330, 486, 523
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 2 cut(s) 460, 744
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 1 cut(s) 680
BseDI CCNNGG 4 cut(s) 328, 528, 534, 591
BseGI GGATG 4 cut(s) 144, 197, 358, 516
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 3 cut(s) 330, 486, 523
BseMI GCAATG 2 cut(s) 460, 744
BseMII CTCAG 2 cut(s) 264, 537
BseRI GAGGAG 1 cut(s) 551
BseXI GCAGC 2 cut(s) 47, 375
BsgI GTGCAG 1 cut(s) 454
Bsh1285I CGRYCG 1 cut(s) 70
BshFI GGCC 4 cut(s) 120, 527, 533, 812
BshNI GGYRCC 2 cut(s) 334, 541
BsiEI CGRYCG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 809
BslI CCNNNNNNNGG 3 cut(s) 330, 486, 523
BsmAI GTCTC 1 cut(s) 670
BsnI GGCC 4 cut(s) 120, 527, 533, 812
Bsp119I TTCGAA 2 cut(s) 101, 749
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 5 cut(s) 59, 184, 339, 360, 577
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 6 cut(s) 70, 255, 262, 366, 392, 480
BspANI GGCC 4 cut(s) 120, 527, 533, 812
BspCNI CTCAG 2 cut(s) 263, 538
BspLI GGNNCC 4 cut(s) 336, 543, 757, 767
BspMI ACCTGC 1 cut(s) 779
BspOI GCTAGC 1 cut(s) 453
BspPI GGATC 2 cut(s) 67, 585
BspT104I TTCGAA 2 cut(s) 101, 749
BspT107I GGYRCC 2 cut(s) 334, 541
BsrDI GCAATG 2 cut(s) 460, 744
BssECI CCNNGG 4 cut(s) 328, 528, 534, 591
BssMI GATC 5 cut(s) 59, 184, 339, 360, 577
BssSI CACGAG 1 cut(s) 383
BssT1I CCWWGG 3 cut(s) 328, 528, 591
Bst2BI CACGAG 1 cut(s) 383
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 1 cut(s) 142
Bst6I CTCTTC 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 505
BstBI TTCGAA 2 cut(s) 101, 749
BstC8I GCNNGC 4 cut(s) 451, 484, 488, 585
BstDEI CTNAG 2 cut(s) 250, 546
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 4 cut(s) 144, 197, 358, 516
BstKTI GATC 5 cut(s) 62, 187, 342, 363, 580
BstMAI GTCTC 1 cut(s) 670
BstMBI GATC 5 cut(s) 59, 184, 339, 360, 577
BstMCI CGRYCG 1 cut(s) 70
BstMWI GCNNNNNNNGC 2 cut(s) 374, 455
BstNI CCWGG 1 cut(s) 680
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 1 cut(s) 678
BstSFI CTRYAG 1 cut(s) 415
BstV1I GCAGC 2 cut(s) 47, 375
BstX2I RGATCY 2 cut(s) 184, 577
BstYI RGATCY 2 cut(s) 184, 577
Bsu36I CCTNAGG 1 cut(s) 546
BsuRI GGCC 4 cut(s) 120, 527, 533, 812
BtgI CCRYGG 1 cut(s) 328
BtrI CACGTC 1 cut(s) 607
BtsCI GGATG 4 cut(s) 144, 197, 358, 516
BtsIMutI CAGTG 1 cut(s) 138
BveI ACCTGC 1 cut(s) 779
Cac8I GCNNGC 4 cut(s) 451, 484, 488, 585
Cfr13I GGNCC 1 cut(s) 525
Csp6I GTAC 4 cut(s) 412, 445, 466, 542
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 5 cut(s) 73, 329, 442, 584, 662
CviQI GTAC 4 cut(s) 412, 445, 466, 542
DdeI CTNAG 2 cut(s) 250, 546
DpnI GATC 5 cut(s) 61, 186, 341, 362, 579
DpnII GATC 5 cut(s) 59, 184, 339, 360, 577
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco130I CCWWGG 3 cut(s) 328, 528, 591
Eco147I AGGCCT 1 cut(s) 120
Eco24I GRGCYC 1 cut(s) 518
Eco72I CACGTG 1 cut(s) 505
Eco81I CCTNAGG 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 678
EcoT14I CCWWGG 3 cut(s) 328, 528, 591
EcoT38I GRGCYC 1 cut(s) 518
ErhI CCWWGG 3 cut(s) 328, 528, 591
FaeI CATG 5 cut(s) 76, 332, 445, 587, 665
FaiI YATR 6 cut(s) 64, 74, 330, 443, 585, 663
FatI CATG 5 cut(s) 72, 328, 441, 583, 661
Fnu4HI GCNGC 5 cut(s) 36, 255, 389, 392, 480
FokI GGATG 4 cut(s) 131, 184, 365, 523
FriOI GRGCYC 1 cut(s) 518
Fsp4HI GCNGC 5 cut(s) 36, 255, 389, 392, 480
FspBI CTAG 2 cut(s) 450, 734
GluI GCNGC 5 cut(s) 36, 255, 389, 392, 480
HaeIII GGCC 4 cut(s) 120, 527, 533, 812
HapII CCGG 1 cut(s) 809
Hin1II CATG 5 cut(s) 76, 332, 445, 587, 665
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 6 cut(s) 98, 111, 128, 438, 494, 698
HpaII CCGG 1 cut(s) 809
HphI GGTGA 2 cut(s) 313, 355
Hpy166II GTNNAC 2 cut(s) 163, 277
Hpy188I TCNGA 3 cut(s) 127, 184, 625
Hpy188III TCNNGA 4 cut(s) 57, 557, 777, 796
Hpy8I GTNNAC 2 cut(s) 163, 277
Hpy99I CGWCG 2 cut(s) 296, 783
HpyAV CCTTC 2 cut(s) 422, 620
HpyCH4III ACNGT 1 cut(s) 142
HpyCH4IV ACGT 2 cut(s) 504, 606
HpyCH4V TGCA 6 cut(s) 4, 50, 471, 583, 587, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 455
HpyF3I CTNAG 2 cut(s) 250, 546
HpySE526I ACGT 2 cut(s) 504, 606
Hsp92II CATG 5 cut(s) 76, 332, 445, 587, 665
KpnI GGTACC 1 cut(s) 545
Kzo9I GATC 5 cut(s) 59, 184, 339, 360, 577
LmnI GCTCC 1 cut(s) 771
Lsp1109I GCAGC 2 cut(s) 47, 375
LweI GCATC 2 cut(s) 37, 144
MaeI CTAG 2 cut(s) 450, 734
MaeII ACGT 2 cut(s) 504, 606
MaeIII GTNAC 1 cut(s) 319
MalI GATC 5 cut(s) 61, 186, 341, 362, 579
MboI GATC 5 cut(s) 59, 184, 339, 360, 577
MboII GAAGA 3 cut(s) 115, 304, 572
MflI RGATCY 2 cut(s) 184, 577
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 5 cut(s) 16, 42, 89, 153, 770
MlyI GAGTC 2 cut(s) 120, 447
MmeI TCCRAC 1 cut(s) 618
MroXI GAANNNNTTC 1 cut(s) 89
MseI TTAA 1 cut(s) 823
MspA1I CMGCKG 2 cut(s) 368, 486
MspI CCGG 1 cut(s) 809
MspR9I CCNGG 1 cut(s) 680
MvaI CCWGG 1 cut(s) 680
MwoI GCNNNNNNNGC 2 cut(s) 374, 455
NcoI CCATGG 1 cut(s) 328
NdeII GATC 5 cut(s) 59, 184, 339, 360, 577
NheI GCTAGC 1 cut(s) 449
NlaIII CATG 5 cut(s) 76, 332, 445, 587, 665
NlaIV GGNNCC 4 cut(s) 336, 543, 757, 767
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 1 cut(s) 319
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 2 cut(s) 101, 749
PaeI GCATGC 1 cut(s) 587
PceI AGGCCT 1 cut(s) 120
PdmI GAANNNNTTC 1 cut(s) 89
PfeI GAWTC 4 cut(s) 98, 128, 494, 698
PkrI GCNGC 5 cut(s) 37, 256, 390, 393, 481
PleI GAGTC 2 cut(s) 119, 446
PmaCI CACGTG 1 cut(s) 505
PmlI CACGTG 1 cut(s) 505
PpsI GAGTC 2 cut(s) 119, 446
Ppu21I YACGTR 1 cut(s) 505
Psp6I CCWGG 1 cut(s) 678
PspCI CACGTG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 4 cut(s) 336, 543, 757, 767
PspPI GGNCC 1 cut(s) 525
PsuI RGATCY 2 cut(s) 184, 577
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 4 cut(s) 413, 446, 467, 543
RsaNI GTAC 4 cut(s) 412, 445, 466, 542
SaqAI TTAA 1 cut(s) 823
SatI GCNGC 5 cut(s) 36, 255, 389, 392, 480
Sau3AI GATC 5 cut(s) 59, 184, 339, 360, 577
Sau96I GGNCC 1 cut(s) 525
ScaI AGTACT 1 cut(s) 413
SchI GAGTC 2 cut(s) 120, 447
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 1 cut(s) 518
SfaNI GCATC 2 cut(s) 37, 144
SfcI CTRYAG 1 cut(s) 415
SfuI TTCGAA 2 cut(s) 101, 749
SphI GCATGC 1 cut(s) 587
Sse9I AATT 5 cut(s) 16, 42, 89, 153, 770
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 6 cut(s) 70, 255, 262, 366, 392, 480
SspMI CTAG 2 cut(s) 450, 734
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 3 cut(s) 328, 528, 591
TaaI ACNGT 1 cut(s) 142
TaiI ACGT 2 cut(s) 507, 609
TaqI TCGA 4 cut(s) 93, 101, 231, 749
TasI AATT 5 cut(s) 16, 42, 89, 153, 770
TatI WGTACW 2 cut(s) 411, 465
TauI GCSGC 3 cut(s) 257, 394, 482
TfiI GAWTC 4 cut(s) 98, 128, 494, 698
Tru1I TTAA 1 cut(s) 823
Tru9I TTAA 1 cut(s) 823
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 2 cut(s) 35, 388
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 3 cut(s) 89, 369, 441
TspGWI ACGGA 2 cut(s) 280, 334
TspRI CASTG 1 cut(s) 145
XapI RAATTY 2 cut(s) 89, 153
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 89
XspI CTAG 2 cut(s) 450, 734
ZrmI AGTACT 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.