pycom12g03150

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
2834596 .. 2839384
4789 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
ATGTCGTCTCTGCTGTATCTCTTTTTCGTACTCATCTCTTTTAATACACCAACAATAGTCGAACTCAGGAAATCGGTCATGACGACATCAGTACGTATATGGAACGGGAAAACAGTGCATGTTCGAAGGCGGGGTTTTCTTGGTGCAGGGATCTTGCAGAGGCATCGACCTTGGCATTCAGTCGGTGAGCTGCTTGAACAAATCCCAGTCAGGACGCTGGGCTCAGGAGGATCGAGTGTCGTGGTCCCACGAAACTTCAGATTGCTGGAGGAACTTGAACGAGGAGAAAAAGGTATTGGGGATGGCACTGTCAGCTATGGGATGGATGACGGAGATGACATCTACATGCGTTCTTGGACTGGCACCATTATTGGTCCCCACAATACTGTACATGAAGGTCGGATCTATCAGTTGAAATTGTTCTGTGATAAAGATTACCCTGAGAAGCCACCAAGTGTTCGTTTTCATTCTCGGGTTAACATGACTTGTGTTAACCATGAAACTGGAGTGGTGGAACCAAAGAAGTTTGGACTTCTTGCAAACTGGCAGCGGGAGTACACCATGGAAGATATTCTTACACAGCTGAAAAAGGAGATGGGAGCCCCGCATAACCGGAAGTTGGTCCAGCCTCCAGAAGGTACCTACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

217

Amino Acids

24.67

Weight (kDa)

9.25

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 114 - 206 1.5e-13 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 638
AccB1I GGYRCC 2 cut(s) 362, 638
AciI CCGC 3 cut(s) 130, 550, 605
AclWI GGATC 3 cut(s) 158, 238, 410
AcuI CTGAAG 1 cut(s) 241
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 5 cut(s) 30, 93, 390, 557, 640
AfiI CCNNNNNNNGG 2 cut(s) 619, 635
AgsI TTSAA 3 cut(s) 197, 278, 415
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 3 cut(s) 190, 315, 583
AluI AGCT 3 cut(s) 190, 315, 583
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 3 cut(s) 158, 238, 410
Ama87I CYCGRG 1 cut(s) 471
ApeKI GCWGC 2 cut(s) 190, 547
Asp700I GAANNNNTTC 2 cut(s) 419, 570
Asp718I GGTACC 1 cut(s) 638
AspS9I GGNCC 3 cut(s) 244, 374, 622
AsuHPI GGTGA 1 cut(s) 197
AsuII TTCGAA 1 cut(s) 124
AvaI CYCGRG 1 cut(s) 471
AvaII GGWCC 3 cut(s) 244, 374, 622
BanI GGYRCC 2 cut(s) 362, 638
BanII GRGCYC 2 cut(s) 224, 604
BbvI GCAGC 2 cut(s) 177, 559
BccI CCATC 3 cut(s) 296, 316, 589
BcgI CGANNNNNNTGC 2 cut(s) 146, 180
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 1 cut(s) 649
BisI GCNGC 2 cut(s) 191, 548
BlsI GCNGC 2 cut(s) 192, 549
Bme18I GGWCC 3 cut(s) 244, 374, 622
BmeT110I CYCGRG 1 cut(s) 471
BmgT120I GGNCC 3 cut(s) 244, 374, 622
BmiI GGNNCC 6 cut(s) 246, 364, 376, 516, 601, 640
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 200
BpmI CTGGAG 3 cut(s) 287, 525, 615
Bpu10I CCTNAGC 1 cut(s) 223
Bpu14I TTCGAA 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 170, 561
BsaWI WCCGGW 1 cut(s) 612
Bsc4I CCNNNNNNNGG 2 cut(s) 619, 635
Bse1I ACTGG 4 cut(s) 206, 364, 508, 548
BseDI CCNNGG 2 cut(s) 170, 561
BseGI GGATG 3 cut(s) 307, 327, 331
BseLI CCNNNNNNNGG 2 cut(s) 619, 635
BseMII CTCAG 3 cut(s) 79, 237, 432
BseNI ACTGG 4 cut(s) 206, 364, 508, 548
BseRI GAGGAG 1 cut(s) 297
BseXI GCAGC 2 cut(s) 177, 559
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 165
BshNI GGYRCC 2 cut(s) 362, 638
BsiHKCI CYCGRG 1 cut(s) 471
BsiSI CCGG 1 cut(s) 613
BslFI GGGAC 2 cut(s) 230, 360
BslI CCNNNNNNNGG 2 cut(s) 619, 635
BsmAI GTCTC 1 cut(s) 12
BsmBI CGTCTC 1 cut(s) 12
BsmFI GGGAC 2 cut(s) 230, 360
BsmI GAATGC 1 cut(s) 175
BsoBI CYCGRG 1 cut(s) 471
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 224, 604
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 3 cut(s) 150, 230, 402
Bsp19I CCATGG 1 cut(s) 561
BspACI CCGC 3 cut(s) 130, 550, 605
BspCNI CTCAG 3 cut(s) 78, 236, 433
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 6 cut(s) 246, 364, 376, 516, 601, 640
BspPI GGATC 3 cut(s) 158, 238, 410
BspT104I TTCGAA 1 cut(s) 124
BspT107I GGYRCC 2 cut(s) 362, 638
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 4 cut(s) 206, 364, 508, 548
BssECI CCNNGG 2 cut(s) 170, 561
BssMI GATC 3 cut(s) 150, 230, 402
BssT1I CCWWGG 2 cut(s) 170, 561
Bst4CI ACNGT 3 cut(s) 115, 310, 388
BstAUI TGTACA 1 cut(s) 388
BstBAI YACGTR 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 124
BstDEI CTNAG 3 cut(s) 65, 223, 441
BstDSI CCRYGG 1 cut(s) 561
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 3 cut(s) 307, 327, 331
BstKTI GATC 3 cut(s) 153, 233, 405
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 3 cut(s) 150, 230, 402
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNSI RCATGY 2 cut(s) 122, 349
BstSNI TACGTA 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 177, 559
BstX2I RGATCY 2 cut(s) 150, 402
BstXI CCANNNNNNTGG 1 cut(s) 503
BstYI RGATCY 2 cut(s) 150, 402
BtgI CCRYGG 1 cut(s) 561
BtsCI GGATG 3 cut(s) 307, 327, 331
BtsIMutI CAGTG 2 cut(s) 120, 306
CciI TCATGA 1 cut(s) 78
Cfr13I GGNCC 3 cut(s) 244, 374, 622
CseI GACGC 1 cut(s) 223
Csp6I GTAC 5 cut(s) 29, 92, 389, 556, 639
CviAII CATG 7 cut(s) 79, 119, 346, 392, 481, 497, 562
CviJI RGCY 7 cut(s) 190, 222, 315, 448, 583, 602, 628
CviKI_1 RGCY 7 cut(s) 190, 222, 315, 448, 583, 602, 628
CviQI GTAC 5 cut(s) 29, 92, 389, 556, 639
DdeI CTNAG 3 cut(s) 65, 223, 441
DpnI GATC 3 cut(s) 152, 232, 404
DpnII GATC 3 cut(s) 150, 230, 402
DraIII CACNNNGTG 1 cut(s) 455
Eco105I TACGTA 1 cut(s) 95
Eco130I CCWWGG 2 cut(s) 170, 561
Eco24I GRGCYC 2 cut(s) 224, 604
Eco47I GGWCC 3 cut(s) 244, 374, 622
Eco57I CTGAAG 1 cut(s) 241
Eco88I CYCGRG 1 cut(s) 471
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoT14I CCWWGG 2 cut(s) 170, 561
EcoT38I GRGCYC 2 cut(s) 224, 604
ErhI CCWWGG 2 cut(s) 170, 561
Esp3I CGTCTC 1 cut(s) 12
FaeI CATG 7 cut(s) 82, 122, 349, 395, 484, 500, 565
FalI AAGNNNNNCTT 2 cut(s) 558, 590
FaqI GGGAC 2 cut(s) 230, 360
FatI CATG 7 cut(s) 78, 118, 345, 391, 480, 496, 561
FauI CCCGC 3 cut(s) 123, 543, 612
Fnu4HI GCNGC 2 cut(s) 191, 548
FokI GGATG 3 cut(s) 314, 334, 338
FriOI GRGCYC 2 cut(s) 224, 604
Fsp4HI GCNGC 2 cut(s) 191, 548
FspBI CTAG 1 cut(s) 649
GluI GCNGC 2 cut(s) 191, 548
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 3 cut(s) 287, 525, 615
HapII CCGG 1 cut(s) 613
HgaI GACGC 1 cut(s) 223
Hin1II CATG 7 cut(s) 82, 122, 349, 395, 484, 500, 565
HincII GTYRAC 2 cut(s) 478, 493
HindII GTYRAC 2 cut(s) 478, 493
HpaI GTTAAC 2 cut(s) 478, 493
HpaII CCGG 1 cut(s) 613
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 478, 493, 558
Hpy188I TCNGA 2 cut(s) 260, 402
Hpy188III TCNNGA 5 cut(s) 67, 79, 211, 225, 632
Hpy8I GTNNAC 3 cut(s) 478, 493, 558
HpyAV CCTTC 3 cut(s) 120, 389, 629
HpyCH4III ACNGT 3 cut(s) 115, 310, 388
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 4 cut(s) 118, 146, 157, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 3 cut(s) 65, 223, 441
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 7 cut(s) 82, 122, 349, 395, 484, 500, 565
KpnI GGTACC 1 cut(s) 642
KspAI GTTAAC 2 cut(s) 478, 493
Kzo9I GATC 3 cut(s) 150, 230, 402
LmnI GCTCC 1 cut(s) 599
Lsp1109I GCAGC 2 cut(s) 177, 559
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 649
MaeII ACGT 1 cut(s) 94
MalI GATC 3 cut(s) 152, 232, 404
MboI GATC 3 cut(s) 150, 230, 402
MboII GAAGA 1 cut(s) 578
MflI RGATCY 2 cut(s) 150, 402
MhlI GDGCHC 2 cut(s) 224, 604
MluCI AATT 1 cut(s) 416
MmeI TCCRAC 1 cut(s) 380
MnlI CCTC 5 cut(s) 153, 221, 262, 275, 639
MroXI GAANNNNTTC 2 cut(s) 419, 570
MseI TTAA 3 cut(s) 42, 477, 492
MslI CAYNNNNRTG 1 cut(s) 344
MspA1I CMGCKG 2 cut(s) 550, 583
MspI CCGG 1 cut(s) 613
Mva1269I GAATGC 1 cut(s) 175
MwoI GCNNNNNNNGC 1 cut(s) 312
NcoI CCATGG 1 cut(s) 561
NdeII GATC 3 cut(s) 150, 230, 402
NlaIII CATG 7 cut(s) 82, 122, 349, 395, 484, 500, 565
NlaIV GGNNCC 6 cut(s) 246, 364, 376, 516, 601, 640
NspI RCATGY 2 cut(s) 122, 349
NspV TTCGAA 1 cut(s) 124
PagI TCATGA 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PctI GAATGC 1 cut(s) 175
PdmI GAANNNNTTC 2 cut(s) 419, 570
PkrI GCNGC 2 cut(s) 192, 549
Ppu21I YACGTR 1 cut(s) 95
PspFI CCCAGC 1 cut(s) 217
PspN4I GGNNCC 6 cut(s) 246, 364, 376, 516, 601, 640
PspPI GGNCC 3 cut(s) 244, 374, 622
PsuI RGATCY 2 cut(s) 150, 402
PvuII CAGCTG 1 cut(s) 583
RsaI GTAC 5 cut(s) 30, 93, 390, 557, 640
RsaNI GTAC 5 cut(s) 29, 92, 389, 556, 639
RseI CAYNNNNRTG 1 cut(s) 344
SaqAI TTAA 3 cut(s) 42, 477, 492
SatI GCNGC 2 cut(s) 191, 548
Sau3AI GATC 3 cut(s) 150, 230, 402
Sau96I GGNCC 3 cut(s) 244, 374, 622
SduI GDGCHC 2 cut(s) 224, 604
SetI ASST 9 cut(s) 97, 172, 192, 295, 317, 400, 585, 640, 644
SfaNI GCATC 1 cut(s) 172
SfuI TTCGAA 1 cut(s) 124
SinI GGWCC 3 cut(s) 244, 374, 622
SmiMI CAYNNNNRTG 1 cut(s) 344
SnaBI TACGTA 1 cut(s) 95
Sse9I AATT 1 cut(s) 416
SsiI CCGC 3 cut(s) 130, 550, 605
SspMI CTAG 1 cut(s) 649
StyI CCWWGG 2 cut(s) 170, 561
TaaI ACNGT 3 cut(s) 115, 310, 388
TaiI ACGT 1 cut(s) 97
TaqI TCGA 4 cut(s) 60, 124, 166, 233
TaqII GACCGA 1 cut(s) 64
TasI AATT 1 cut(s) 416
TatI WGTACW 2 cut(s) 388, 555
Tru1I TTAA 3 cut(s) 42, 477, 492
Tru9I TTAA 3 cut(s) 42, 477, 492
TscAI CASTG 2 cut(s) 120, 313
TseI GCWGC 2 cut(s) 190, 547
TspDTI ATGAA 3 cut(s) 408, 455, 513
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 120, 313
VpaK11BI GGWCC 3 cut(s) 244, 374, 622
XagI CCTNNNNNAGG 1 cut(s) 633
XceI RCATGY 2 cut(s) 122, 349
XmnI GAANNNNTTC 2 cut(s) 419, 570
XspI CTAG 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.