pycom12g04650

protein ROOT PRIMORDIUM DEFECTIVE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
4489310 .. 4489870
561 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGAATTTTCCGAGGGGTTATGGTGGGCAGAAGAAGGTGAGGGCATGGATGGAGGAGTTTCAGAAGTTACCATATATTTCACCATATGAGGATTCTAGACATATTGATCCTAAGAGTGATCTTATGGAGAAAAGGGTGGTTGGGGTTTTGCACGAGTTCTTAAGCTTGACTCTTCATAAGAAGACTAAGAGGAACTACTCGAGAAGCTTGAGGGAGGACTTGAATCTTCCTCATAAGTTTACGAGAATCATCACAAGGTATCCCGGGATTTTCTACCTCTCGTTGAAGTGTAAGACCGCCATTGTTTCCCTCAGGGAAGGGTACCATAGAGGGAAGTTAGTGAACCCACATCTGCTCACACGTTTGAGAGAGAAGTTTTATCACGTCATGAGAACAGGGCTTCTTTATCAGGGTAAAGGTGTGAACTTGCTATCCCCGGATGAGATTTTGCTTAACAGTGTGGAGAGTAAAAGTGGGCAGGAAGCACCTGAAGAGGAAGAGGTTGGAACAGGTGATGAATGTGAGGATGAAGTATTGGATTCAGGTTCGGAACGGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

187

Amino Acids

21.57

Weight (kDa)

8.76

Isoelectric Point (pI)

55.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 1 - 133 3.9e-54 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 321
AccB1I GGYRCC 1 cut(s) 321
AciI CCGC 1 cut(s) 297
AclWI GGATC 1 cut(s) 101
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 1 cut(s) 323
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 359
AgsI TTSAA 2 cut(s) 223, 286
AjiI CACGTC 1 cut(s) 385
AloI GAACNNNNNNTCC 2 cut(s) 416, 448
AluBI AGCT 2 cut(s) 165, 207
AluI AGCT 2 cut(s) 165, 207
AlwI GGATC 1 cut(s) 101
Ama87I CYCGRG 2 cut(s) 199, 263
ApoI RAATTY 1 cut(s) 4
Asp718I GGTACC 1 cut(s) 321
AsuC2I CCSGG 3 cut(s) 264, 265, 437
AsuHPI GGTGA 3 cut(s) 49, 72, 524
AvaI CYCGRG 2 cut(s) 199, 263
AxyI CCTNAGG 1 cut(s) 311
BanI GGYRCC 1 cut(s) 321
BauI CACGAG 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 188
BccI CCATC 1 cut(s) 43
BciVI GTATCC 1 cut(s) 270
BcnI CCSGG 3 cut(s) 264, 265, 437
BfaI CTAG 1 cut(s) 96
BfrI CTTAAG 1 cut(s) 160
BfuI GTATCC 1 cut(s) 270
Bme1390I CCNGG 3 cut(s) 264, 265, 437
BmeT110I CYCGRG 2 cut(s) 199, 263
BmgBI CACGTC 1 cut(s) 385
BmiI GGNNCC 1 cut(s) 323
BmrFI CCNGG 3 cut(s) 264, 265, 437
BpiI GAAGAC 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 229
BpuMI CCSGG 3 cut(s) 264, 265, 437
BsaJI CCNNGG 3 cut(s) 11, 263, 435
Bse21I CCTNAGG 1 cut(s) 311
BseDI CCNNGG 3 cut(s) 11, 263, 435
BseGI GGATG 3 cut(s) 54, 445, 532
BseMII CTCAG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 68
BshNI GGYRCC 1 cut(s) 321
BsiHKCI CYCGRG 2 cut(s) 199, 263
BsiSI CCGG 2 cut(s) 264, 437
BsoBI CYCGRG 2 cut(s) 199, 263
Bsp143I GATC 2 cut(s) 106, 118
BspACI CCGC 1 cut(s) 297
BspCNI CTCAG 1 cut(s) 324
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 323
BspPI GGATC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 321
BspTI CTTAAG 1 cut(s) 160
BssECI CCNNGG 3 cut(s) 11, 263, 435
BssMI GATC 2 cut(s) 106, 118
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst4CI ACNGT 2 cut(s) 458, 555
Bst6I CTCTTC 3 cut(s) 177, 486, 492
BstAFI CTTAAG 1 cut(s) 160
BstDEI CTNAG 3 cut(s) 111, 186, 311
BstF5I GGATG 3 cut(s) 54, 445, 532
BstKTI GATC 2 cut(s) 109, 121
BstMBI GATC 2 cut(s) 106, 118
BstSCI CCNGG 3 cut(s) 262, 263, 435
BstV2I GAAGAC 1 cut(s) 188
Bsu36I CCTNAGG 1 cut(s) 311
BsuI GTATCC 1 cut(s) 270
BtrI CACGTC 1 cut(s) 385
BtsCI GGATG 3 cut(s) 54, 445, 532
BtsIMutI CAGTG 1 cut(s) 463
CciI TCATGA 1 cut(s) 387
Cfr9I CCCGGG 1 cut(s) 263
Csp6I GTAC 1 cut(s) 322
CviAII CATG 2 cut(s) 45, 388
CviJI RGCY 3 cut(s) 165, 207, 400
CviKI_1 RGCY 3 cut(s) 165, 207, 400
CviQI GTAC 1 cut(s) 322
DdeI CTNAG 3 cut(s) 111, 186, 311
DpnI GATC 2 cut(s) 108, 120
DpnII GATC 2 cut(s) 106, 118
Eam1104I CTCTTC 3 cut(s) 177, 486, 492
EarI CTCTTC 3 cut(s) 177, 486, 492
Eco57I CTGAAG 1 cut(s) 510
Eco81I CCTNAGG 1 cut(s) 311
Eco88I CYCGRG 2 cut(s) 199, 263
FaeI CATG 2 cut(s) 48, 391
FatI CATG 2 cut(s) 44, 387
FauNDI CATATG 1 cut(s) 85
FokI GGATG 3 cut(s) 61, 452, 539
FspBI CTAG 1 cut(s) 96
HapII CCGG 2 cut(s) 264, 437
Hin1II CATG 2 cut(s) 48, 391
HindIII AAGCTT 2 cut(s) 163, 205
HinfI GANTC 5 cut(s) 92, 169, 223, 246, 539
HpaII CCGG 2 cut(s) 264, 437
HphI GGTGA 3 cut(s) 49, 72, 524
Hpy166II GTNNAC 3 cut(s) 240, 343, 424
Hpy188I TCNGA 3 cut(s) 12, 63, 550
Hpy188III TCNNGA 4 cut(s) 96, 201, 388, 558
Hpy8I GTNNAC 3 cut(s) 240, 343, 424
HpyAV CCTTC 2 cut(s) 28, 311
HpyCH4III ACNGT 2 cut(s) 458, 555
HpyCH4IV ACGT 2 cut(s) 361, 384
HpyCH4V TGCA 1 cut(s) 151
HpyF3I CTNAG 3 cut(s) 111, 186, 311
HpySE526I ACGT 2 cut(s) 361, 384
Hsp92II CATG 2 cut(s) 48, 391
KpnI GGTACC 1 cut(s) 325
Kzo9I GATC 2 cut(s) 106, 118
LpnPI CCDG 9 cut(s) 277, 298, 381, 395, 450, 464, 495, 501, 528
MaeI CTAG 1 cut(s) 96
MaeII ACGT 2 cut(s) 361, 384
MaeIII GTNAC 1 cut(s) 66
MalI GATC 2 cut(s) 108, 120
MboI GATC 2 cut(s) 106, 118
MboII GAAGA 6 cut(s) 43, 164, 193, 218, 503, 509
MluCI AATT 1 cut(s) 4
MlyI GAGTC 1 cut(s) 163
MmeI TCCRAC 1 cut(s) 484
MseI TTAA 2 cut(s) 161, 453
MspCI CTTAAG 1 cut(s) 160
MspI CCGG 2 cut(s) 264, 437
MspR9I CCNGG 3 cut(s) 264, 265, 437
NciI CCSGG 3 cut(s) 264, 265, 437
NdeI CATATG 1 cut(s) 85
NdeII GATC 2 cut(s) 106, 118
NlaIII CATG 2 cut(s) 48, 391
NlaIV GGNNCC 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 199
PagI TCATGA 1 cut(s) 387
PfeI GAWTC 4 cut(s) 92, 223, 246, 539
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
PspN4I GGNNCC 1 cut(s) 323
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
SaqAI TTAA 2 cut(s) 161, 453
Sau3AI GATC 2 cut(s) 106, 118
SchI GAGTC 1 cut(s) 163
ScrFI CCNGG 3 cut(s) 264, 265, 437
Sfr274I CTCGAG 1 cut(s) 199
SlaI CTCGAG 1 cut(s) 199
SmaI CCCGGG 1 cut(s) 265
SmlI CTYRAG 3 cut(s) 160, 199, 208
SmoI CTYRAG 3 cut(s) 160, 199, 208
Sse9I AATT 1 cut(s) 4
SsiI CCGC 1 cut(s) 297
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 3 cut(s) 262, 263, 435
TaaI ACNGT 2 cut(s) 458, 555
TaiI ACGT 2 cut(s) 364, 387
TaqI TCGA 1 cut(s) 200
TasI AATT 1 cut(s) 4
TfiI GAWTC 4 cut(s) 92, 223, 246, 539
Tru1I TTAA 2 cut(s) 161, 453
Tru9I TTAA 2 cut(s) 161, 453
TscAI CASTG 1 cut(s) 463
TspDTI ATGAA 4 cut(s) 17, 164, 531, 543
TspMI CCCGGG 1 cut(s) 263
TspRI CASTG 1 cut(s) 463
Vha464I CTTAAG 1 cut(s) 160
XapI RAATTY 1 cut(s) 4
XbaI TCTAGA 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 199
XmaI CCCGGG 1 cut(s) 263
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.