pycom12g05970

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
5951355 .. 5956769
5415 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1566 bp
ATGTACAACACATCAACTCAAATTCAAACGCCCACTCGATCTACAAAACAATTATATCTTAAAACTATCAGTAATAAGTTAACTACGTCTCAAGCAAAAAGTCAACGCCAAGTTGTCCGTGGCAAACATTGCGTACCTGCGTGGGGACGTAGAAGGGGAATCTTCTTATTTTCTCTTATCCTCCTTTCTTCTTCTTCCACCTCTATTTTTCTTCTCTTTTTTATTTTTATTTATTTATATTATTTTTTGGCTCCTCCCTGCAAACTGCGAAGCGAAGATTTTGACCGATCGGACAAAATGGTGAAGGAAACGGAGTACTACGACGTTCTCGGCGTCAGCCCGGCGGCGACCGAGGCGGAGATCAAGAAAGCTTATTACATGAAGGCAAGGCAGGTTCATCCAGATAAAAACCCAAATGATCCACTCGCAGCACAAAATTTTCAGGTTTTGGGAGAAGCTTACCAAGTGTTAAGCGACCCAAGCCAACGCCAAGCATATGATGCTTATGGAAAATCAGGAATTTCAACCGACGCAATTATTGATCCTGCAGCAATCTTTGCCATGCTTTTTGGTAGTGAGCTCTTTGAGGAATATATTGGACAGCTAGCCATGGCATCAATGGCTTCCTTAGATATTTTCACAGAAGGGGAACAGTTTGATGCGAAAAAGTTGCAAGACAAAATGCGGGTTGTTCAGAAGGAAAGAGAAGAAAAGCTCGCAGAAACACTAAAAAATCGACTCAACCAATATGTGCAAGGCAACAAAGAGGAATTTGTTAGTCATGCTGAAGCTGAAGTTGTGAGGCTTTCCAATGCAGCTTATGGTGTTGATATGTTGAATACAATTGGCTACATATATGCCAGACAATCAGCAAAGGAGCTAGGCAAGAAAGCAATGTATTTGGGTGTGCCATTTGTTGCAGAGTGGTTTAGAAATAAAGGGCATTTCATTAAATCTCAAGTAACAGCAGCAACAGGTGCAATTGCTTTGATCCAGCTGCAGGAAGACATGAAAAAGCAGCTAAGTGCTGAAGGAAATTACACAGAGGAAGAGCTCGAAGAATACATGCAATCTCACAAGAAAGTGATGATTGATTCCCTGTGGAAGCTTAATGTGGCTGATATTGAGGCCACTCTTTCTCGTGTTTGTCAGATGGTTCTTCAAGACAACAATGCCAAGAAAGAGGAACTCCGTGCACGAGCAAAGGGGTTGAAAACTCTTGGCAAAATCTTCCAGAGAACTAAGTCAACCGGTGGGAATGAGGGTGAAACTCCACAAAATAGTAGAGTTCATAAACTGAATGGAAGTGAATTACGCTATGATCCCTCTTCTCCTATAACAACACCAAAGTCCTCAAAGCCTGAAGAGCCATCATATACTGCATTTGAGCCGCAGTTCACAGAGCCCCTATGTGGAAGCCCCCCAAATTGCAGGAGCGCAGTTGGACTATCACTTCCCAAGGCCAACCGCACCACCTGGTGCCCAGAGACATTCTTCCATTGGCAGGGATTAAGGGTGGAGATTAGGTTTGCTCCTTGCTTTCCGTTTTGTAGTAAAAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

522

Amino Acids

58.97

Weight (kDa)

8.88

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 105 - 167 3.1e-25 DnaJ domain
DnaJ-X PF14308 232 - 423 6e-56 X-domain of DnaJ-containing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 145, 382
AccB1I GGYRCC 1 cut(s) 1479
AciI CCGC 5 cut(s) 344, 356, 685, 1391, 1468
AclWI GGATC 4 cut(s) 413, 536, 985, 1316
AcsI RAATTY 4 cut(s) 21, 436, 519, 770
AcuI CTGAAG 4 cut(s) 807, 813, 1050, 1383
AcyI GRCGYC 1 cut(s) 333
AdeI CACNNNGTG 1 cut(s) 1479
AfaI GTAC 3 cut(s) 5, 135, 317
AfiI CCNNNNNNNGG 4 cut(s) 143, 1000, 1412, 1504
AgeI ACCGGT 1 cut(s) 1250
AgsI TTSAA 5 cut(s) 26, 525, 838, 1163, 1213
AjnI CCWGG 1 cut(s) 1475
Alw21I GWGCWC 3 cut(s) 582, 1056, 1198
Alw26I GTCTC 2 cut(s) 93, 1481
Alw44I GTGCAC 1 cut(s) 1194
AlwI GGATC 4 cut(s) 413, 536, 985, 1316
AoxI GGCC 2 cut(s) 1128, 1461
ApaLI GTGCAC 1 cut(s) 1194
ApeKI GCWGC 6 cut(s) 428, 548, 815, 968, 997, 1018
ApoI RAATTY 4 cut(s) 21, 436, 519, 770
AsiGI ACCGGT 1 cut(s) 1250
AspLEI GCGC 1 cut(s) 1439
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 313, 1277
AsuNHI GCTAGC 1 cut(s) 604
BaeGI GKGCMC 2 cut(s) 1198, 1484
BanI GGYRCC 1 cut(s) 1479
BanII GRGCYC 3 cut(s) 582, 1056, 1407
BauI CACGAG 2 cut(s) 1140, 1197
BbsI GAAGAC 1 cut(s) 1011
Bbv12I GWGCWC 3 cut(s) 582, 1056, 1198
BbvI GCAGC 6 cut(s) 440, 560, 827, 980, 984, 1030
BccI CCATC 2 cut(s) 1147, 1378
BcgI CGANNNNNNTGC 2 cut(s) 652, 686
BciT130I CCWGG 1 cut(s) 1477
BcnI CCSGG 1 cut(s) 341
BcoDI GTCTC 2 cut(s) 93, 1481
BfaI CTAG 2 cut(s) 605, 881
BfmI CTRYAG 2 cut(s) 546, 998
BfuAI ACCTGC 2 cut(s) 145, 382
BisI GCNGC 8 cut(s) 345, 429, 549, 816, 969, 998, 1019, 1391
BlsI GCNGC 8 cut(s) 346, 430, 550, 817, 970, 999, 1020, 1392
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 2 cut(s) 341, 1477
BmiI GGNNCC 2 cut(s) 252, 1481
BmrFI CCNGG 2 cut(s) 341, 1477
BmsI GCATC 3 cut(s) 490, 623, 649
BmtI GCTAGC 1 cut(s) 608
BpiI GAAGAC 1 cut(s) 1011
BpuEI CTTGAG 2 cut(s) 75, 942
BpuMI CCSGG 1 cut(s) 341
BsaHI GRCGYC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 118, 351, 609, 1458
BsaWI WCCGGW 1 cut(s) 1250
BsaXI ACNNNNNCTCC 2 cut(s) 1511, 1541
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 1000, 1412, 1504
Bse118I RCCGGY 1 cut(s) 1250
Bse3DI GCAATG 2 cut(s) 127, 900
BseBI CCWGG 1 cut(s) 1477
BseDI CCNNGG 4 cut(s) 118, 351, 609, 1458
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 4 cut(s) 143, 1000, 1412, 1504
BseMI GCAATG 2 cut(s) 127, 900
BseRI GAGGAG 1 cut(s) 243
BseSI GKGCMC 2 cut(s) 1198, 1484
BseXI GCAGC 6 cut(s) 440, 560, 827, 980, 984, 1030
Bsh1285I CGRYCG 2 cut(s) 290, 351
BshFI GGCC 2 cut(s) 1130, 1463
BshNI GGYRCC 1 cut(s) 1479
BshTI ACCGGT 1 cut(s) 1250
BsiEI CGRYCG 2 cut(s) 290, 351
BsiHKAI GWGCWC 3 cut(s) 582, 1056, 1198
BsiSI CCGG 2 cut(s) 341, 1251
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 4 cut(s) 143, 1000, 1412, 1504
BsmAI GTCTC 2 cut(s) 93, 1481
BsmBI CGTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 159
BsnI GGCC 2 cut(s) 1130, 1463
Bsp1286I GDGCHC 5 cut(s) 582, 1056, 1198, 1407, 1484
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 5 cut(s) 344, 356, 685, 1391, 1468
BspANI GGCC 2 cut(s) 1130, 1463
BspLI GGNNCC 2 cut(s) 252, 1481
BspMAI CTGCAG 2 cut(s) 550, 1002
BspMI ACCTGC 2 cut(s) 145, 382
BspOI GCTAGC 1 cut(s) 608
BspPI GGATC 4 cut(s) 413, 536, 985, 1316
BspQI GCTCTTC 2 cut(s) 1044, 1359
BspT107I GGYRCC 1 cut(s) 1479
BsrDI GCAATG 2 cut(s) 127, 900
BsrFI RCCGGY 1 cut(s) 1250
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 1 cut(s) 1250
BssECI CCNNGG 4 cut(s) 118, 351, 609, 1458
BssMI GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
BssNI GRCGYC 1 cut(s) 333
BssSI CACGAG 2 cut(s) 1140, 1197
BssT1I CCWWGG 2 cut(s) 609, 1458
Bst2BI CACGAG 2 cut(s) 1140, 1197
Bst2UI CCWGG 1 cut(s) 1477
Bst4CI ACNGT 1 cut(s) 654
Bst6I CTCTTC 3 cut(s) 1044, 1333, 1359
BstACI GRCGYC 1 cut(s) 333
BstAPI GCANNNNNTGC 4 cut(s) 129, 500, 557, 977
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 606, 717
BstDEI CTNAG 3 cut(s) 628, 1022, 1242
BstDSI CCRYGG 2 cut(s) 118, 609
BstF5I GGATG 1 cut(s) 397
BstHHI GCGC 1 cut(s) 1439
BstKTI GATC 7 cut(s) 41, 290, 363, 421, 544, 993, 1324
BstMAI GTCTC 2 cut(s) 93, 1481
BstMBI GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
BstMCI CGRYCG 2 cut(s) 290, 351
BstMWI GCNNNNNNNGC 8 cut(s) 129, 353, 480, 500, 557, 620, 977, 1366
BstNI CCWGG 1 cut(s) 1477
BstNSI RCATGY 1 cut(s) 1069
BstSCI CCNGG 2 cut(s) 339, 1475
BstSFI CTRYAG 2 cut(s) 546, 998
BstSLI GKGCMC 2 cut(s) 1198, 1484
BstV1I GCAGC 6 cut(s) 440, 560, 827, 980, 984, 1030
BstV2I GAAGAC 1 cut(s) 1011
BsuRI GGCC 2 cut(s) 1130, 1463
BtgI CCRYGG 2 cut(s) 118, 609
BtsCI GGATG 1 cut(s) 397
BveI ACCTGC 2 cut(s) 145, 382
Cac8I GCNNGC 2 cut(s) 606, 717
CfoI GCGC 1 cut(s) 1439
Cfr10I RCCGGY 1 cut(s) 1250
CseI GACGC 2 cut(s) 322, 539
CsiI ACCWGGT 1 cut(s) 1475
Csp6I GTAC 3 cut(s) 4, 134, 316
CspAI ACCGGT 1 cut(s) 1250
CviAII CATG 6 cut(s) 379, 562, 610, 782, 1009, 1066
CviQI GTAC 3 cut(s) 4, 134, 316
DdeI CTNAG 3 cut(s) 628, 1022, 1242
DpnI GATC 7 cut(s) 40, 289, 362, 420, 543, 992, 1323
DpnII GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
DraIII CACNNNGTG 1 cut(s) 1479
Eam1104I CTCTTC 3 cut(s) 1044, 1333, 1359
EarI CTCTTC 3 cut(s) 1044, 1333, 1359
EciI GGCGGA 1 cut(s) 371
Ecl136II GAGCTC 2 cut(s) 580, 1054
Eco130I CCWWGG 2 cut(s) 609, 1458
Eco24I GRGCYC 3 cut(s) 582, 1056, 1407
Eco53kI GAGCTC 2 cut(s) 580, 1054
Eco57I CTGAAG 4 cut(s) 807, 813, 1050, 1383
EcoICRI GAGCTC 2 cut(s) 580, 1054
EcoRII CCWGG 1 cut(s) 1475
EcoT14I CCWWGG 2 cut(s) 609, 1458
EcoT38I GRGCYC 3 cut(s) 582, 1056, 1407
ErhI CCWWGG 2 cut(s) 609, 1458
Esp3I CGTCTC 1 cut(s) 93
FaeI CATG 6 cut(s) 382, 565, 613, 785, 1012, 1069
FaqI GGGAC 1 cut(s) 159
FatI CATG 6 cut(s) 378, 561, 609, 781, 1008, 1065
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 496
Fnu4HI GCNGC 8 cut(s) 345, 429, 549, 816, 969, 998, 1019, 1391
FokI GGATG 1 cut(s) 384
FriOI GRGCYC 3 cut(s) 582, 1056, 1407
Fsp4HI GCNGC 8 cut(s) 345, 429, 549, 816, 969, 998, 1019, 1391
FspBI CTAG 2 cut(s) 605, 881
GlaI GCGC 1 cut(s) 1438
GluI GCNGC 8 cut(s) 345, 429, 549, 816, 969, 998, 1019, 1391
HaeIII GGCC 2 cut(s) 1130, 1463
HapII CCGG 2 cut(s) 341, 1251
HgaI GACGC 2 cut(s) 322, 539
HhaI GCGC 1 cut(s) 1439
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 6 cut(s) 382, 565, 613, 785, 1012, 1069
Hin6I GCGC 1 cut(s) 1437
HinP1I GCGC 1 cut(s) 1437
HincII GTYRAC 3 cut(s) 81, 104, 1248
HindII GTYRAC 3 cut(s) 81, 104, 1248
HindIII AAGCTT 3 cut(s) 369, 456, 1106
HinfI GANTC 3 cut(s) 159, 738, 1094
HpaI GTTAAC 1 cut(s) 81
HpaII CCGG 2 cut(s) 341, 1251
HphI GGTGA 2 cut(s) 313, 1277
Hpy166II GTNNAC 5 cut(s) 81, 104, 1196, 1248, 1398
Hpy188I TCNGA 3 cut(s) 292, 696, 1152
Hpy188III TCNNGA 5 cut(s) 364, 401, 516, 1163, 1234
Hpy8I GTNNAC 5 cut(s) 81, 104, 1196, 1248, 1398
Hpy99I CGWCG 2 cut(s) 326, 533
HpyAV CCTTC 6 cut(s) 147, 298, 376, 638, 691, 1025
HpyCH4III ACNGT 1 cut(s) 654
HpyCH4IV ACGT 3 cut(s) 86, 148, 324
HpyF10VI GCNNNNNNNGC 8 cut(s) 129, 353, 480, 500, 557, 620, 977, 1366
HpyF3I CTNAG 3 cut(s) 628, 1022, 1242
HpySE526I ACGT 3 cut(s) 86, 148, 324
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 6 cut(s) 382, 565, 613, 785, 1012, 1069
HspAI GCGC 1 cut(s) 1437
KspAI GTTAAC 1 cut(s) 81
Kzo9I GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
LguI GCTCTTC 2 cut(s) 1044, 1359
LmnI GCTCC 4 cut(s) 256, 877, 1434, 1537
Lsp1109I GCAGC 6 cut(s) 440, 560, 827, 980, 984, 1030
LweI GCATC 3 cut(s) 490, 623, 649
MabI ACCWGGT 1 cut(s) 1475
MaeI CTAG 2 cut(s) 605, 881
MaeII ACGT 3 cut(s) 86, 148, 324
MaeIII GTNAC 1 cut(s) 961
MalI GATC 7 cut(s) 40, 289, 362, 420, 543, 992, 1323
MboI GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
MfeI CAATTG 2 cut(s) 843, 981
MhlI GDGCHC 5 cut(s) 582, 1056, 1198, 1407, 1484
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 1 cut(s) 1423
MseI TTAA 6 cut(s) 60, 80, 470, 951, 1110, 1511
MspA1I CMGCKG 1 cut(s) 997
MspI CCGG 2 cut(s) 341, 1251
MspR9I CCNGG 2 cut(s) 341, 1477
MunI CAATTG 2 cut(s) 843, 981
MvaI CCWGG 1 cut(s) 1477
MwoI GCNNNNNNNGC 8 cut(s) 129, 353, 480, 500, 557, 620, 977, 1366
NciI CCSGG 1 cut(s) 341
NcoI CCATGG 1 cut(s) 609
NdeI CATATG 1 cut(s) 496
NdeII GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
NheI GCTAGC 1 cut(s) 604
NlaIII CATG 6 cut(s) 382, 565, 613, 785, 1012, 1069
NlaIV GGNNCC 2 cut(s) 252, 1481
NmeAIII GCCGAG 1 cut(s) 309
NspI RCATGY 1 cut(s) 1069
PciSI GCTCTTC 2 cut(s) 1044, 1359
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PfeI GAWTC 2 cut(s) 159, 1094
PinAI ACCGGT 1 cut(s) 1250
PkrI GCNGC 8 cut(s) 346, 430, 550, 817, 970, 999, 1020, 1392
Ple19I CGATCG 1 cut(s) 290
PleI GAGTC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 732
Psp124BI GAGCTC 2 cut(s) 582, 1056
Psp6I CCWGG 1 cut(s) 1475
PspGI CCWGG 1 cut(s) 1475
PspN4I GGNNCC 2 cut(s) 252, 1481
PstI CTGCAG 2 cut(s) 550, 1002
PvuI CGATCG 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 997
RsaI GTAC 3 cut(s) 5, 135, 317
RsaNI GTAC 3 cut(s) 4, 134, 316
SacI GAGCTC 2 cut(s) 582, 1056
SapI GCTCTTC 2 cut(s) 1044, 1359
SaqAI TTAA 6 cut(s) 60, 80, 470, 951, 1110, 1511
SatI GCNGC 8 cut(s) 345, 429, 549, 816, 969, 998, 1019, 1391
Sau3AI GATC 7 cut(s) 38, 287, 360, 418, 541, 990, 1321
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 732
ScrFI CCNGG 2 cut(s) 341, 1477
SduI GDGCHC 5 cut(s) 582, 1056, 1198, 1407, 1484
SexAI ACCWGGT 1 cut(s) 1475
SfaNI GCATC 3 cut(s) 490, 623, 649
SfcI CTRYAG 2 cut(s) 546, 998
SmlI CTYRAG 2 cut(s) 90, 957
SmoI CTYRAG 2 cut(s) 90, 957
SsiI CCGC 5 cut(s) 344, 356, 685, 1391, 1468
SspMI CTAG 2 cut(s) 605, 881
SstI GAGCTC 2 cut(s) 582, 1056
StyD4I CCNGG 2 cut(s) 339, 1475
StyI CCWWGG 2 cut(s) 609, 1458
TaaI ACNGT 1 cut(s) 654
TaiI ACGT 3 cut(s) 89, 151, 327
TaqI TCGA 3 cut(s) 37, 736, 1056
TaqII GACCGA 2 cut(s) 300, 365
TatI WGTACW 2 cut(s) 3, 315
TauI GCSGC 2 cut(s) 347, 1393
TfiI GAWTC 2 cut(s) 159, 1094
Tru1I TTAA 6 cut(s) 60, 80, 470, 951, 1110, 1511
Tru9I TTAA 6 cut(s) 60, 80, 470, 951, 1110, 1511
TseI GCWGC 6 cut(s) 428, 548, 815, 968, 997, 1018
TspDTI ATGAA 5 cut(s) 386, 395, 937, 1025, 1280
TspGWI ACGGA 4 cut(s) 107, 326, 1181, 1533
VneI GTGCAC 1 cut(s) 1194
XapI RAATTY 4 cut(s) 21, 436, 519, 770
XceI RCATGY 1 cut(s) 1069
XcmI CCANNNNNNNNNTGG 2 cut(s) 116, 616
XspI CTAG 2 cut(s) 605, 881
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.