pycom12g06890
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
7151698 .. 7153425
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGACATGTCCTCATACCCCACAACAAAATGGGGTTACTGAGAGGAAAAATTGCCATATCTTAGAAACAGCACGTGCTCTCCTTCTTGGGGCATCTGTTCCTAAGGTGTTTTGGCCTGAAGCTGTCACTTATGCAGTCTATGTGATTAATCATTACCAGGGGGAGAGCGCTAAAGGTGATCTGAAATGGTTTGACCTAGGGGGAGACAATATTGCAGAGACCGCACCTGCTAGTGGAGAAATTGTTACCGAGAGTAGTATGCAGTCACAGCAACCACCTACCGAATCTGCTGATGTGCCCCCCTCTCTCTCTAGCTCCACTGTGCTGCCCAATTTGTCTTCTTTGGATATACATGAGGTACCAACCCGTGTGGAGGAAGCTCTAAAGGATCCAAAGTGGGCAAAAGCAATGGATGAAGAAATGTTGGCGCTACAGAAGAGTAATACCTGGGAGGTGATGCCGTTGCCAAAAGGAAAGAGAACAGTTGGATGCCGATGGTTTGATGTGAAGAATACTTTTCTCCATGGGAACTTGGAGGAAGAAGTATATATGGATTTTCCTCTTGCGTATAGTGCTAGTGGAAATACCAGAGAAAAAGTGACAGCATTAATTATCTATGTAGATGACATGATTATCACTGGAGATGATATTGATGGCTACTGTTATGCGTATCTTAGCATATCTGAAGTCTGCACCAGGAAAATGAGTACTATATGGGAAACATGGACATTTAAGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

247

Amino Acids

27.43

Weight (kDa)

4.8

Isoelectric Point (pI)

50.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
Acc65I GGTACC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 358
AciI CCGC 1 cut(s) 222
AclWI GGATC 2 cut(s) 383, 396
AcuI CTGAAG 2 cut(s) 138, 705
AcvI CACGTG 1 cut(s) 74
AfaI GTAC 2 cut(s) 360, 709
AfeI AGCGCT 1 cut(s) 169
AfiI CCNNNNNNNGG 3 cut(s) 88, 233, 373
AflIII ACRYGT 1 cut(s) 5
AjnI CCWGG 3 cut(s) 156, 446, 695
AluBI AGCT 3 cut(s) 122, 315, 380
AluI AGCT 3 cut(s) 122, 315, 380
Alw21I GWGCWC 1 cut(s) 79
Alw26I GTCTC 2 cut(s) 198, 212
AlwI GGATC 2 cut(s) 383, 396
Aor51HI AGCGCT 1 cut(s) 169
AoxI GGCC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 325
AseI ATTAAT 2 cut(s) 147, 608
Asp718I GGTACC 1 cut(s) 358
AspA2I CCTAGG 1 cut(s) 196
AspLEI GCGC 2 cut(s) 170, 430
AsuHPI GGTGA 2 cut(s) 188, 466
AvrII CCTAGG 1 cut(s) 196
AxyI CCTNAGG 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 300
BamHI GGATCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 358
BbrPI CACGTG 1 cut(s) 74
BbsI GAAGAC 1 cut(s) 330
Bbv12I GWGCWC 1 cut(s) 79
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 489, 647
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 3 cut(s) 158, 448, 697
BcoDI GTCTC 2 cut(s) 198, 212
BfaI CTAG 4 cut(s) 197, 231, 312, 576
BfmI CTRYAG 1 cut(s) 431
BfoI RGCGCY 2 cut(s) 171, 431
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 1 cut(s) 326
BlnI CCTAGG 1 cut(s) 196
BlsI GCNGC 1 cut(s) 327
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 3 cut(s) 158, 448, 697
BmiI GGNNCC 2 cut(s) 360, 390
BmrFI CCNGG 3 cut(s) 158, 448, 697
BmsI GCATC 3 cut(s) 101, 447, 479
BpiI GAAGAC 1 cut(s) 330
BpmI CTGGAG 1 cut(s) 660
BsaAI YACGTR 1 cut(s) 74
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 4 cut(s) 157, 196, 447, 523
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 228, 258
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 233, 373
Bse1I ACTGG 1 cut(s) 643
Bse21I CCTNAGG 1 cut(s) 102
Bse3DI GCAATG 1 cut(s) 414
BseBI CCWGG 3 cut(s) 158, 448, 697
BseDI CCNNGG 4 cut(s) 157, 196, 447, 523
BseGI GGATG 2 cut(s) 418, 494
BseLI CCNNNNNNNGG 3 cut(s) 88, 233, 373
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 643
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 676
BshFI GGCC 1 cut(s) 115
BshNI GGYRCC 1 cut(s) 358
BsiHKAI GWGCWC 1 cut(s) 79
BslI CCNNNNNNNGG 3 cut(s) 88, 233, 373
BsmAI GTCTC 2 cut(s) 198, 212
BsnI GGCC 1 cut(s) 115
Bso31I GGTCTC 1 cut(s) 212
Bsp1286I GDGCHC 2 cut(s) 79, 300
Bsp143I GATC 2 cut(s) 178, 388
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 1 cut(s) 222
BspANI GGCC 1 cut(s) 115
BspCNI CTCAG 1 cut(s) 31
BspLI GGNNCC 2 cut(s) 360, 390
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 2 cut(s) 383, 396
BspT107I GGYRCC 1 cut(s) 358
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 1 cut(s) 414
BsrI ACTGG 1 cut(s) 643
BssECI CCNNGG 4 cut(s) 157, 196, 447, 523
BssMI GATC 2 cut(s) 178, 388
BssT1I CCWWGG 2 cut(s) 196, 523
Bst2UI CCWGG 3 cut(s) 158, 448, 697
Bst4CI ACNGT 3 cut(s) 322, 484, 662
Bst6I CTCTTC 1 cut(s) 431
BstBAI YACGTR 1 cut(s) 74
BstDEI CTNAG 4 cut(s) 39, 61, 102, 674
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 2 cut(s) 418, 494
BstH2I RGCGCY 2 cut(s) 171, 431
BstHHI GCGC 2 cut(s) 170, 430
BstKTI GATC 2 cut(s) 181, 391
BstMAI GTCTC 2 cut(s) 198, 212
BstMBI GATC 2 cut(s) 178, 388
BstMWI GCNNNNNNNGC 3 cut(s) 221, 268, 572
BstNI CCWGG 3 cut(s) 158, 448, 697
BstNSI RCATGY 1 cut(s) 9
BstSCI CCNGG 3 cut(s) 156, 446, 695
BstSFI CTRYAG 1 cut(s) 431
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 330
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 102
BsuRI GGCC 1 cut(s) 115
BtgI CCRYGG 1 cut(s) 523
BtsCI GGATG 2 cut(s) 418, 494
BtsIMutI CAGTG 2 cut(s) 318, 636
BveI ACCTGC 1 cut(s) 235
CfoI GCGC 2 cut(s) 170, 430
Csp6I GTAC 2 cut(s) 359, 708
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviAII CATG 5 cut(s) 6, 353, 524, 628, 723
CviJI RGCY 5 cut(s) 115, 122, 315, 380, 657
CviKI_1 RGCY 5 cut(s) 115, 122, 315, 380, 657
CviQI GTAC 2 cut(s) 359, 708
DdeI CTNAG 4 cut(s) 39, 61, 102, 674
DpnI GATC 2 cut(s) 180, 390
DpnII GATC 2 cut(s) 178, 388
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
Eco130I CCWWGG 2 cut(s) 196, 523
Eco31I GGTCTC 1 cut(s) 212
Eco47III AGCGCT 1 cut(s) 169
Eco57I CTGAAG 2 cut(s) 138, 705
Eco72I CACGTG 1 cut(s) 74
Eco81I CCTNAGG 1 cut(s) 102
EcoRII CCWGG 3 cut(s) 156, 446, 695
EcoT14I CCWWGG 2 cut(s) 196, 523
ErhI CCWWGG 2 cut(s) 196, 523
FaeI CATG 5 cut(s) 9, 356, 527, 631, 726
FatI CATG 5 cut(s) 5, 352, 523, 627, 722
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 2 cut(s) 425, 501
Fsp4HI GCNGC 1 cut(s) 326
FspBI CTAG 4 cut(s) 197, 231, 312, 576
GlaI GCGC 2 cut(s) 169, 429
GluI GCNGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 660
HaeII RGCGCY 2 cut(s) 171, 431
HaeIII GGCC 1 cut(s) 115
HhaI GCGC 2 cut(s) 170, 430
Hin1II CATG 5 cut(s) 9, 356, 527, 631, 726
Hin6I GCGC 2 cut(s) 168, 428
HinP1I GCGC 2 cut(s) 168, 428
HinfI GANTC 1 cut(s) 284
HphI GGTGA 2 cut(s) 188, 466
Hpy188I TCNGA 2 cut(s) 183, 685
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 3 cut(s) 322, 484, 662
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 4 cut(s) 134, 215, 262, 693
HpyF10VI GCNNNNNNNGC 3 cut(s) 221, 268, 572
HpyF3I CTNAG 4 cut(s) 39, 61, 102, 674
HpySE526I ACGT 1 cut(s) 73
Hsp92II CATG 5 cut(s) 9, 356, 527, 631, 726
HspAI GCGC 2 cut(s) 168, 428
KpnI GGTACC 1 cut(s) 362
Kzo9I GATC 2 cut(s) 178, 388
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 3 cut(s) 101, 447, 479
MaeI CTAG 4 cut(s) 197, 231, 312, 576
MaeII ACGT 1 cut(s) 73
MaeIII GTNAC 5 cut(s) 34, 124, 244, 264, 598
MalI GATC 2 cut(s) 180, 390
MboI GATC 2 cut(s) 178, 388
MboII GAAGA 5 cut(s) 330, 428, 448, 520, 551
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 79, 300
MluCI AATT 4 cut(s) 49, 240, 331, 609
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 8 cut(s) 21, 36, 313, 349, 367, 445, 529, 570
MseI TTAA 3 cut(s) 147, 608, 732
MspR9I CCNGG 3 cut(s) 158, 448, 697
MvaI CCWGG 3 cut(s) 158, 448, 697
MwoI GCNNNNNNNGC 3 cut(s) 221, 268, 572
NcoI CCATGG 1 cut(s) 523
NdeII GATC 2 cut(s) 178, 388
NlaIII CATG 5 cut(s) 9, 356, 527, 631, 726
NlaIV GGNNCC 2 cut(s) 360, 390
NmuCI GTSAC 3 cut(s) 124, 264, 598
NspI RCATGY 1 cut(s) 9
PaqCI CACCTGC 1 cut(s) 235
PciI ACATGT 1 cut(s) 5
PfeI GAWTC 1 cut(s) 284
PkrI GCNGC 1 cut(s) 327
PmaCI CACGTG 1 cut(s) 74
PmlI CACGTG 1 cut(s) 74
Ppu21I YACGTR 1 cut(s) 74
PscI ACATGT 1 cut(s) 5
PshBI ATTAAT 2 cut(s) 147, 608
Psp6I CCWGG 3 cut(s) 156, 446, 695
PspCI CACGTG 1 cut(s) 74
PspGI CCWGG 3 cut(s) 156, 446, 695
PspN4I GGNNCC 2 cut(s) 360, 390
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 2 cut(s) 360, 709
RsaNI GTAC 2 cut(s) 359, 708
SaqAI TTAA 3 cut(s) 147, 608, 732
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 2 cut(s) 178, 388
ScaI AGTACT 1 cut(s) 709
ScrFI CCNGG 3 cut(s) 158, 448, 697
SduI GDGCHC 2 cut(s) 79, 300
SfaNI GCATC 3 cut(s) 101, 447, 479
SfcI CTRYAG 1 cut(s) 431
Sse9I AATT 4 cut(s) 49, 240, 331, 609
SsiI CCGC 1 cut(s) 222
SspI AATATT 1 cut(s) 211
SspMI CTAG 4 cut(s) 197, 231, 312, 576
StyD4I CCNGG 3 cut(s) 156, 446, 695
StyI CCWWGG 2 cut(s) 196, 523
TaaI ACNGT 3 cut(s) 322, 484, 662
TaiI ACGT 1 cut(s) 76
TasI AATT 4 cut(s) 49, 240, 331, 609
TatI WGTACW 1 cut(s) 707
TfiI GAWTC 1 cut(s) 284
Tru1I TTAA 3 cut(s) 147, 608, 732
Tru9I TTAA 3 cut(s) 147, 608, 732
TscAI CASTG 2 cut(s) 325, 643
TseFI GTSAC 3 cut(s) 124, 264, 598
TseI GCWGC 1 cut(s) 325
Tsp45I GTSAC 3 cut(s) 124, 264, 598
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 2 cut(s) 325, 643
VspI ATTAAT 2 cut(s) 147, 608
XceI RCATGY 1 cut(s) 9
XmaJI CCTAGG 1 cut(s) 196
XspI CTAG 4 cut(s) 197, 231, 312, 576
ZrmI AGTACT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.