pycom12g08280

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
9408403 .. 9410283
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
ATGCTTCTTAACTTTGTAATAGTTGTTGCACAGGATTGGGTGGGCCAGAGAGACGTTAGTTGGATCACAATTATGGTGGAATTGGGAAGAAGTAGATTCAACAAGAAGCCAAAAGGAAGGAGAAATCTCTTCGAGGGAGCAAAGAAGCTGGACATAATAGTGAAAGACGCGGATGGAGAAATGTATATGGTTCCAAATACAGCTTTTGATGTAGGAATGCTGAATTTGACACATCCCGATACTGAAAGTTGGTTCAGGCAAATTCTTCGGGTAATGATGGATGATCGAGTCAGAGGATGGACTCAAGTTAAGAGAACTAAAGCCACAATTTTGTCGTCCCTCAATAAAACCTTCATACGCTTACGTTTGGCAGGTATAAATCTTAACAAACCATTTTGCAACAGCCAATCCTACTCAAACGACCCAACTCTGTCGCATTTTGCACGCTTTGCTAAGATTTATAAATCTTGCAAGTTCTATAGCATCCAGCTTGTATTGATTGTTCCAAGGTTTCTTCTATACTATACCAATTGTATACAACATCTATCAACTTCACCAGAAAATCTGACTAATTGTTTACCTCTTTGCATCTTTCTAACCGGCACTGAGATTCTAGTGATGCCAATCCTAGAAAAAGGCAAGAATAATGTGAAGGCCTATTTTCCAAGAGGAGGAGAAACTTCTTGTTGGGAACACATATTGAGTGGAAAACAATATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_31

Protein Analysis

241

Amino Acids

27.67

Weight (kDa)

9.6

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 362
AccI GTMKAC 1 cut(s) 535
AccII CGCG 1 cut(s) 170
AciI CCGC 1 cut(s) 170
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 2 cut(s) 223, 261
AfiI CCNNNNNNNGG 1 cut(s) 671
AgsI TTSAA 1 cut(s) 100
AluBI AGCT 3 cut(s) 148, 203, 490
AluI AGCT 3 cut(s) 148, 203, 490
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 1 cut(s) 71
AoxI GGCC 2 cut(s) 43, 654
ApoI RAATTY 2 cut(s) 223, 261
AspS9I GGNCC 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 546
BccI CCATC 3 cut(s) 167, 271, 291
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 2 cut(s) 614, 629
BfmI CTRYAG 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 1 cut(s) 192
BmsI GCATC 3 cut(s) 492, 597, 609
BpuEI CTTGAG 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 623
BsaJI CCNNGG 1 cut(s) 506
Bsc4I CCNNNNNNNGG 1 cut(s) 671
Bse118I RCCGGY 1 cut(s) 599
Bse8I GATNNNNATC 1 cut(s) 623
BseDI CCNNGG 1 cut(s) 506
BseGI GGATG 5 cut(s) 178, 232, 286, 302, 483
BseJI GATNNNNATC 1 cut(s) 623
BseLI CCNNNNNNNGG 1 cut(s) 671
BseMII CTCAG 1 cut(s) 597
BseRI GAGGAG 2 cut(s) 684, 687
Bsh1236I CGCG 1 cut(s) 170
BshFI GGCC 2 cut(s) 45, 656
BsiSI CCGG 1 cut(s) 600
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 1 cut(s) 671
BsmAI GTCTC 1 cut(s) 45
BsmBI CGTCTC 1 cut(s) 45
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 222
BsnI GGCC 2 cut(s) 45, 656
Bsp143I GATC 2 cut(s) 63, 283
BspACI CCGC 1 cut(s) 170
BspANI GGCC 2 cut(s) 45, 656
BspCNI CTCAG 1 cut(s) 598
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 1 cut(s) 192
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 1 cut(s) 71
BsrFI RCCGGY 1 cut(s) 599
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 1 cut(s) 506
BssMI GATC 2 cut(s) 63, 283
BssNAI GTATAC 1 cut(s) 536
BssT1I CCWWGG 1 cut(s) 506
Bst1107I GTATAC 1 cut(s) 536
Bst6I CTCTTC 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 453, 606
BstF5I GGATG 5 cut(s) 178, 232, 286, 302, 483
BstFNI CGCG 1 cut(s) 170
BstKTI GATC 2 cut(s) 66, 286
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 2 cut(s) 63, 283
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstSFI CTRYAG 1 cut(s) 478
BstUI CGCG 1 cut(s) 170
BstZ17I GTATAC 1 cut(s) 536
BsuRI GGCC 2 cut(s) 45, 656
BtsCI GGATG 5 cut(s) 178, 232, 286, 302, 483
BtsIMutI CAGTG 1 cut(s) 603
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 1 cut(s) 445
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 43
CseI GACGC 1 cut(s) 176
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviJI RGCY 8 cut(s) 45, 109, 148, 203, 323, 405, 490, 656
CviKI_1 RGCY 8 cut(s) 45, 109, 148, 203, 323, 405, 490, 656
DdeI CTNAG 2 cut(s) 453, 606
DpnI GATC 2 cut(s) 65, 285
DpnII GATC 2 cut(s) 63, 283
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Eco130I CCWWGG 1 cut(s) 506
Eco147I AGGCCT 1 cut(s) 656
EcoT14I CCWWGG 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 45
FaqI GGGAC 1 cut(s) 322
FblI GTMKAC 1 cut(s) 535
FokI GGATG 5 cut(s) 185, 219, 293, 309, 470
FspBI CTAG 2 cut(s) 614, 629
HaeIII GGCC 2 cut(s) 45, 656
HapII CCGG 1 cut(s) 600
HgaI GACGC 1 cut(s) 176
HinfI GANTC 4 cut(s) 96, 288, 301, 610
HpaII CCGG 1 cut(s) 600
HphI GGTGA 1 cut(s) 546
Hpy166II GTNNAC 2 cut(s) 536, 578
Hpy188I TCNGA 2 cut(s) 293, 567
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 2 cut(s) 536, 578
HpyAV CCTTC 3 cut(s) 111, 361, 646
HpyCH4IV ACGT 2 cut(s) 54, 364
HpyCH4V TGCA 5 cut(s) 29, 399, 443, 471, 588
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpyF3I CTNAG 2 cut(s) 453, 606
HpySE526I ACGT 2 cut(s) 54, 364
Kzo9I GATC 2 cut(s) 63, 283
LmnI GCTCC 1 cut(s) 137
LpnPI CCDG 8 cut(s) 17, 59, 134, 241, 357, 500, 570, 613
LweI GCATC 3 cut(s) 492, 597, 609
MaeI CTAG 2 cut(s) 614, 629
MaeII ACGT 2 cut(s) 54, 364
MalI GATC 2 cut(s) 65, 285
MboI GATC 2 cut(s) 63, 283
MboII GAAGA 4 cut(s) 99, 121, 257, 506
MfeI CAATTG 1 cut(s) 529
MluCI AATT 7 cut(s) 69, 80, 223, 261, 327, 529, 571
MlyI GAGTC 2 cut(s) 295, 297
MmeI TCCRAC 1 cut(s) 41
MnlI CCTC 6 cut(s) 127, 287, 350, 591, 662, 665
MseI TTAA 3 cut(s) 9, 309, 384
MslI CAYNNNNRTG 2 cut(s) 71, 158
MspI CCGG 1 cut(s) 600
MunI CAATTG 1 cut(s) 529
Mva1269I GAATGC 1 cut(s) 222
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 2 cut(s) 63, 283
NlaIV GGNNCC 1 cut(s) 192
PceI AGGCCT 1 cut(s) 656
PctI GAATGC 1 cut(s) 222
PfeI GAWTC 2 cut(s) 96, 610
PleI GAGTC 2 cut(s) 295, 296
PpsI GAGTC 2 cut(s) 295, 296
PsiI TTATAA 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 192
PspPI GGNCC 1 cut(s) 43
PsrI GAACNNNNNNTAC 2 cut(s) 486, 518
RseI CAYNNNNRTG 2 cut(s) 71, 158
SaqAI TTAA 3 cut(s) 9, 309, 384
Sau3AI GATC 2 cut(s) 63, 283
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 2 cut(s) 295, 297
SetI ASST 9 cut(s) 57, 150, 205, 353, 367, 376, 492, 512, 583
SfaNI GCATC 3 cut(s) 492, 597, 609
SfcI CTRYAG 1 cut(s) 478
SmiMI CAYNNNNRTG 2 cut(s) 71, 158
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
Sse9I AATT 7 cut(s) 69, 80, 223, 261, 327, 529, 571
SseBI AGGCCT 1 cut(s) 656
SsiI CCGC 1 cut(s) 170
SspI AATATT 1 cut(s) 716
SspMI CTAG 2 cut(s) 614, 629
StuI AGGCCT 1 cut(s) 656
StyI CCWWGG 1 cut(s) 506
TaiI ACGT 2 cut(s) 57, 367
TaqI TCGA 2 cut(s) 132, 286
TasI AATT 7 cut(s) 69, 80, 223, 261, 327, 529, 571
TfiI GAWTC 2 cut(s) 96, 610
Tru1I TTAA 3 cut(s) 9, 309, 384
Tru9I TTAA 3 cut(s) 9, 309, 384
TscAI CASTG 1 cut(s) 610
TspDTI ATGAA 2 cut(s) 343, 708
TspRI CASTG 1 cut(s) 610
XapI RAATTY 2 cut(s) 223, 261
XmiI GTMKAC 1 cut(s) 535
XspI CTAG 2 cut(s) 614, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.