pycom12g09510

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
11460431 .. 11463557
3127 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2286 bp
ATGGCCGATAAAACATATGTCATGTTTACGTGGAAGTTGTTGGGTCTAGGTAAATCTTTCTCCTTGCCCCCGATATGGTTCATAAACTCATCTAACCAAGCTTATGCTTCTTCTGGTAGCATTAGTTCAGGCAAGATAGTATCTATTAACATTGGTGCGGGCAGCCCTCTTTCGGGCGAAATGGTTCCAAAGCAGATCGGTTCTGGGCTCCACCTTTGGGATGGGGATTTGATGGCACATGGCTCATTTCCTCTTCATTTTTTCTGTTTGGCTTCTTCAACTCCTTCTTACTCCTCTAATGAGGCTGATTTCTTTCTTCATGAGGAACAATTTTGGATTTTACATTCTTCGAGGTCTCCGAAGTCTTTGGACCCTCCTAATTATGGAGGCTCCTATCTCTTTAACAGCTTTCCCATCTTTTCCGATTATATACCATTTTTGCCCAAGTCGGGCAAGATTCTTCTTTGTCACCGGGTTTACGGTCCTTCCATTCATTCTAACATCTCCATCATGATAACTTGGTTGGGGTGGCCTCACATCTACCCATCTTGGTACATTGGCCTCCTTGCTGGTAGTTTCCCCCCAACTTCTGGAAGACTCTTGCTCTTTTTTTTATTTTTTTTATCAACTCCTGATCAAGGATGGCTCCCGCCGCTGATACCTCTAACTCGCATTCACATTGACATTTACTACAACTTGATAGCTCTTTCAATCAGCTTATCAACTTGCCGTTTCTCAGCTTTTACCTCCCAGGTAGCTTTCCCTTTCCCTTTCTCTGCCTATATTAGGTTAATCATGTTTACTTGGACTAGTAGAAAAGGAATAGTATCAATCACAGTACCAGCATGGGGTTCTTCTAGTGATAGCTTTACTTCTGACCAACACTGCCCCCTGTTATCCAATAACATAAGGTCGGGCAACTCTGGTCCCTTGATTAAACAAGGGAATAACCAGCTTTTCCCCCGGGTTGGCTTCCCTTATTATATTTCCAGAACGATCGATTTCTCTGGGGTAGAACACTTCAGATCTAGCTTTCTTTGCAGACTCTTTGGTGGGGAAAAGAGTCTATTGACCTATTTTCTCGTTTCTAAAGAGTTTATCTCCATGCTTTTTAAGACAACCATAATGGTAGCCTGTAAATCTTCATTTATGACCTTCCCTCCTGATTTCTCAGAAGGAGTCGGGCTTTCCGTTATGACAGTGGTGTGGTTTTTGTTCTTTTTTGCACCTGAATGTTTGTTCTTTCCTTCGTCTTTTTGGCATACAAGATTTGTATCCCACCGGGCATGCCAAAATTATGTTCGGGCAGGAATTTCACTGGGAAGGGTCCCTTGCTCGAGCACACAACTCCCCAAGGAGTTAACACTTTGGTTGACTGTCGAGCTCTTGCTCGCTTGTCGGGCTATAAGAAGAGGACAAAGCCAAGAGGCTTGCAATCTTTTGTCGGGCAAGGTTAAAACGTCTTGCAATCTCTTTTCTTTGCAATTACAAAGGGGGAAAGTATCAGAGCTTTTAAATAGACAAAAGATGTCTTGCTACGGGAAATCTAAGGCTGAAAAGGGTGTAAGATCGATCATGGACAAAGCGCATTGTTTGTTTGATTGGTTGAGTGTCCTTGTCTCTGGGCCTTCTTCCTCCTTTTATAGACAAATTAACCCAATTGTGTTATTTTTCATTTTTACATGTTCTGCCATCCAAAAGTACTTTTGGGCCAGAAGTAGGTGGATTTCCATCGATTGTCACTTCTCTTGTAAGCACCTAGCCTTTGCATTTAATGAAGGCCTGGGCTTCATTCTTTTTGAAGCCCAAAGTTTAAATATTAACCCAAACAGCTGCTATGCTACCGTCTGTCTTCTTAATGTTGTTTTGAGGCTCAAACACTGTGTTGGTCGTGGCACTGCTTGTTTCATTAAGGCGGCGATGAAAAGTTATTATGCTCCACCATGTTGCTTTTTCTCCCTCTTTGGTTTTCAAAATGGTGACCCTCAATTCCAATTCTCTTTCTTTCCTTGTCTCGATTTTCGATTAAATGTGATTGTGCTGGGATGGATCTATGAACAGAGATGGAGGAAAATGAGCTTGGGGAGCACATTTGTTCTGTGGAAATGGAGAGGAAAGAGTGAACGTCATATTTTGACAGCAATAAGGCCAGCAATGTTTTATGGCACAAAATGTTGGGCAGTGAAGCATCAACACGTACATAAAATGGGTGTAGCGGACATGAAAATGCTTCGTTGGATATGTGGGCACACAAGAAATGATAAGATTAGGAATGAAGATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

762

Amino Acids

86.36

Weight (kDa)

9.44

Isoelectric Point (pI)

50.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 590
AciI CCGC 5 cut(s) 158, 650, 653, 1916, 2216
AclWI GGATC 1 cut(s) 2057
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 1311
AcuI CTGAAG 1 cut(s) 1006
AfaI GTAC 4 cut(s) 554, 840, 1703, 2199
AflIII ACRYGT 2 cut(s) 1682, 2194
AgsI TTSAA 4 cut(s) 279, 711, 1802, 1973
AhlI ACTAGT 1 cut(s) 809
AjnI CCWGG 2 cut(s) 750, 1782
AjuI GAANNNNNNNTTGG 4 cut(s) 1284, 1316, 2063, 2095
Alw21I GWGCWC 3 cut(s) 1343, 1386, 2090
Alw26I GTCTC 3 cut(s) 360, 1624, 2018
AlwI GGATC 1 cut(s) 2057
Ama87I CYCGRG 2 cut(s) 963, 1336
AoxI GGCC 7 cut(s) 3, 530, 559, 1625, 1710, 1780, 2147
ApeKI GCWGC 2 cut(s) 162, 1833
ApoI RAATTY 1 cut(s) 1311
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 1588
AspS9I GGNCC 6 cut(s) 370, 482, 926, 1327, 1625, 1710
AsuC2I CCSGG 4 cut(s) 473, 964, 965, 1283
AsuHPI GGTGA 2 cut(s) 461, 1991
AvaI CYCGRG 2 cut(s) 963, 1336
AvaII GGWCC 4 cut(s) 370, 482, 926, 1327
BaeGI GKGCMC 1 cut(s) 2250
BanII GRGCYC 2 cut(s) 210, 1386
BbsI GAAGAC 2 cut(s) 601, 1844
Bbv12I GWGCWC 3 cut(s) 1343, 1386, 2090
BbvI GCAGC 2 cut(s) 174, 1820
BceAI ACGGC 1 cut(s) 714
BciT130I CCWGG 2 cut(s) 752, 1784
BciVI GTATCC 1 cut(s) 1285
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 4 cut(s) 473, 964, 965, 1283
BcoDI GTCTC 3 cut(s) 360, 1624, 2018
BcuI ACTAGT 1 cut(s) 809
BfaI CTAG 5 cut(s) 47, 810, 858, 1029, 1760
BfuI GTATCC 1 cut(s) 1285
BglII AGATCT 1 cut(s) 1025
BisI GCNGC 4 cut(s) 163, 653, 1834, 1917
BlsI GCNGC 4 cut(s) 164, 654, 1835, 1918
BmcAI AGTACT 1 cut(s) 1703
Bme1390I CCNGG 6 cut(s) 473, 752, 964, 965, 1283, 1784
Bme18I GGWCC 4 cut(s) 370, 482, 926, 1327
BmeT110I CYCGRG 2 cut(s) 963, 1336
BmgT120I GGNCC 6 cut(s) 370, 482, 926, 1327, 1625, 1710
BmiI GGNNCC 8 cut(s) 186, 209, 372, 391, 647, 928, 1328, 1329
BmrFI CCNGG 6 cut(s) 473, 752, 964, 965, 1283, 1784
BmrI ACTGGG 1 cut(s) 1328
BmsI GCATC 1 cut(s) 2197
BmuI ACTGGG 1 cut(s) 1328
BpiI GAAGAC 2 cut(s) 601, 1844
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
BpuMI CCSGG 4 cut(s) 473, 964, 965, 1283
Bsa29I ATCGAT 3 cut(s) 999, 1571, 1734
BsaAI YACGTR 2 cut(s) 30, 2197
BsaBI GATNNNNATC 2 cut(s) 1273, 1730
BsaI GGTCTC 1 cut(s) 360
BsaJI CCNNGG 5 cut(s) 750, 962, 963, 1353, 1783
BsaXI ACNNNNNCTCC 2 cut(s) 1144, 1174
Bse1I ACTGG 1 cut(s) 1323
Bse3DI GCAATG 1 cut(s) 2160
Bse8I GATNNNNATC 2 cut(s) 1273, 1730
BseBI CCWGG 2 cut(s) 752, 1784
BseCI ATCGAT 3 cut(s) 999, 1571, 1734
BseDI CCNNGG 5 cut(s) 750, 962, 963, 1353, 1783
BseGI GGATG 4 cut(s) 226, 647, 1692, 2051
BseJI GATNNNNATC 2 cut(s) 1273, 1730
BseMI GCAATG 1 cut(s) 2160
BseMII CTCAG 2 cut(s) 750, 1185
BseNI ACTGG 1 cut(s) 1323
BseRI GAGGAG 1 cut(s) 283
BseSI GKGCMC 1 cut(s) 2250
BseXI GCAGC 2 cut(s) 174, 1820
BseYI CCCAGC 1 cut(s) 2041
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 7 cut(s) 5, 532, 561, 1627, 1712, 1782, 2149
BshVI ATCGAT 3 cut(s) 999, 1571, 1734
BsiEI CGRYCG 1 cut(s) 999
BsiHKAI GWGCWC 3 cut(s) 1343, 1386, 2090
BsiHKCI CYCGRG 2 cut(s) 963, 1336
BsiSI CCGG 3 cut(s) 472, 964, 1282
BslFI GGGAC 2 cut(s) 912, 1313
BsmAI GTCTC 3 cut(s) 360, 1624, 2018
BsmFI GGGAC 2 cut(s) 912, 1313
BsmI GAATGC 1 cut(s) 672
BsnI GGCC 7 cut(s) 5, 532, 561, 1627, 1712, 1782, 2149
Bso31I GGTCTC 1 cut(s) 360
BsoBI CYCGRG 2 cut(s) 963, 1336
Bsp1286I GDGCHC 5 cut(s) 210, 1343, 1386, 2090, 2250
Bsp143I GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
BspACI CCGC 5 cut(s) 158, 650, 653, 1916, 2216
BspANI GGCC 7 cut(s) 5, 532, 561, 1627, 1712, 1782, 2149
BspCNI CTCAG 2 cut(s) 749, 1184
BspDI ATCGAT 3 cut(s) 999, 1571, 1734
BspHI TCATGA 2 cut(s) 319, 510
BspLI GGNNCC 8 cut(s) 186, 209, 372, 391, 647, 928, 1328, 1329
BspPI GGATC 1 cut(s) 2057
BspTNI GGTCTC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 2160
BsrI ACTGG 1 cut(s) 1323
BssECI CCNNGG 5 cut(s) 750, 962, 963, 1353, 1783
BssMI GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
BssT1I CCWWGG 1 cut(s) 1353
Bst2UI CCWGG 2 cut(s) 752, 1784
Bst4CI ACNGT 6 cut(s) 482, 838, 1201, 1378, 1846, 1883
Bst6I CTCTTC 2 cut(s) 258, 1405
BstBAI YACGTR 2 cut(s) 30, 2197
BstC8I GCNNGC 5 cut(s) 160, 1288, 1392, 1432, 2151
BstDEI CTNAG 3 cut(s) 736, 1171, 1548
BstEII GGTNACC 1 cut(s) 1979
BstENI CCTNNNNNAGG 2 cut(s) 636, 784
BstF5I GGATG 4 cut(s) 226, 647, 1692, 2051
BstHHI GCGC 1 cut(s) 1588
BstKTI GATC 7 cut(s) 198, 637, 999, 1028, 1571, 1575, 2052
BstMAI GTCTC 3 cut(s) 360, 1624, 2018
BstMBI GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 4 cut(s) 652, 1038, 1400, 2085
BstNI CCWGG 2 cut(s) 752, 1784
BstNSI RCATGY 2 cut(s) 1290, 1686
BstPI GGTNACC 1 cut(s) 1979
BstSCI CCNGG 6 cut(s) 471, 750, 962, 963, 1281, 1782
BstSLI GKGCMC 1 cut(s) 2250
BstV1I GCAGC 2 cut(s) 174, 1820
BstV2I GAAGAC 2 cut(s) 601, 1844
BstX2I RGATCY 2 cut(s) 1025, 2049
BstYI RGATCY 2 cut(s) 1025, 2049
Bsu15I ATCGAT 3 cut(s) 999, 1571, 1734
BsuI GTATCC 1 cut(s) 1285
BsuRI GGCC 7 cut(s) 5, 532, 561, 1627, 1712, 1782, 2149
BsuTUI ATCGAT 3 cut(s) 999, 1571, 1734
BtgZI GCGATG 1 cut(s) 1934
BtsCI GGATG 4 cut(s) 226, 647, 1692, 2051
BtsI GCAGTG 3 cut(s) 883, 1896, 2187
BtsIMutI CAGTG 6 cut(s) 883, 1206, 1316, 1879, 1896, 2187
Cac8I GCNNGC 5 cut(s) 160, 1288, 1392, 1432, 2151
CciI TCATGA 2 cut(s) 319, 510
CfoI GCGC 1 cut(s) 1588
Cfr13I GGNCC 6 cut(s) 370, 482, 926, 1327, 1625, 1710
Cfr9I CCCGGG 1 cut(s) 963
ClaI ATCGAT 3 cut(s) 999, 1571, 1734
Csp6I GTAC 4 cut(s) 553, 839, 1702, 2198
CviQI GTAC 4 cut(s) 553, 839, 1702, 2198
DdeI CTNAG 3 cut(s) 736, 1171, 1548
DpnI GATC 7 cut(s) 197, 636, 998, 1027, 1570, 1574, 2051
DpnII GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
DraI TTTAAA 2 cut(s) 1515, 1815
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 258, 1405
EarI CTCTTC 2 cut(s) 258, 1405
Ecl136II GAGCTC 1 cut(s) 1384
Eco130I CCWWGG 1 cut(s) 1353
Eco147I AGGCCT 1 cut(s) 1782
Eco24I GRGCYC 2 cut(s) 210, 1386
Eco31I GGTCTC 1 cut(s) 360
Eco32I GATATC 1 cut(s) 2281
Eco47I GGWCC 4 cut(s) 370, 482, 926, 1327
Eco53kI GAGCTC 1 cut(s) 1384
Eco57I CTGAAG 1 cut(s) 1006
Eco88I CYCGRG 2 cut(s) 963, 1336
Eco91I GGTNACC 1 cut(s) 1979
EcoICRI GAGCTC 1 cut(s) 1384
EcoNI CCTNNNNNAGG 2 cut(s) 636, 784
EcoO109I RGGNCCY 1 cut(s) 1327
EcoO65I GGTNACC 1 cut(s) 1979
EcoRII CCWGG 2 cut(s) 750, 1782
EcoRV GATATC 1 cut(s) 2281
EcoT14I CCWWGG 1 cut(s) 1353
EcoT38I GRGCYC 2 cut(s) 210, 1386
ErhI CCWWGG 1 cut(s) 1353
FalI AAGNNNNNCTT 2 cut(s) 1315, 1347
FaqI GGGAC 2 cut(s) 912, 1313
FauI CCCGC 2 cut(s) 151, 657
FauNDI CATATG 1 cut(s) 16
FbaI TGATCA 1 cut(s) 634
Fnu4HI GCNGC 4 cut(s) 163, 653, 1834, 1917
FokI GGATG 4 cut(s) 233, 654, 1679, 2058
FriOI GRGCYC 2 cut(s) 210, 1386
Fsp4HI GCNGC 4 cut(s) 163, 653, 1834, 1917
FspBI CTAG 5 cut(s) 47, 810, 858, 1029, 1760
GlaI GCGC 1 cut(s) 1587
GluI GCNGC 4 cut(s) 163, 653, 1834, 1917
GsaI CCCAGC 1 cut(s) 2045
HaeIII GGCC 7 cut(s) 5, 532, 561, 1627, 1712, 1782, 2149
HapII CCGG 3 cut(s) 472, 964, 1282
HhaI GCGC 1 cut(s) 1588
Hin6I GCGC 1 cut(s) 1586
HinP1I GCGC 1 cut(s) 1586
HincII GTYRAC 2 cut(s) 1362, 1374
HindII GTYRAC 2 cut(s) 1362, 1374
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 5 cut(s) 457, 597, 1044, 1063, 1179
HpaI GTTAAC 1 cut(s) 1362
HpaII CCGG 3 cut(s) 472, 964, 1282
HphI GGTGA 2 cut(s) 461, 1991
Hpy166II GTNNAC 6 cut(s) 27, 478, 801, 1362, 1374, 2123
Hpy188I TCNGA 7 cut(s) 360, 424, 877, 1025, 1174, 1507, 2285
Hpy188III TCNNGA 7 cut(s) 320, 511, 591, 632, 990, 1163, 2015
Hpy8I GTNNAC 6 cut(s) 27, 478, 801, 1362, 1374, 2123
HpyAV CCTTC 8 cut(s) 294, 495, 1165, 1169, 1257, 1317, 1638, 1772
HpyCH4III ACNGT 6 cut(s) 482, 838, 1201, 1378, 1846, 1883
HpyCH4IV ACGT 4 cut(s) 29, 1460, 2125, 2196
HpyCH4V TGCA 6 cut(s) 1041, 1226, 1434, 1467, 1483, 1769
HpyF10VI GCNNNNNNNGC 4 cut(s) 652, 1038, 1400, 2085
HpyF3I CTNAG 3 cut(s) 736, 1171, 1548
HpySE526I ACGT 4 cut(s) 29, 1460, 2125, 2196
HspAI GCGC 1 cut(s) 1586
KflI GGGWCCC 1 cut(s) 1327
Ksp22I TGATCA 1 cut(s) 634
KspAI GTTAAC 1 cut(s) 1362
Kzo9I GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
LmnI GCTCC 5 cut(s) 213, 395, 651, 1942, 2085
Lsp1109I GCAGC 2 cut(s) 174, 1820
LweI GCATC 1 cut(s) 2197
MaeI CTAG 5 cut(s) 47, 810, 858, 1029, 1760
MaeII ACGT 4 cut(s) 29, 1460, 2125, 2196
MaeIII GTNAC 3 cut(s) 467, 1739, 1979
MalI GATC 7 cut(s) 197, 636, 998, 1027, 1570, 1574, 2051
MboI GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
MfeI CAATTG 1 cut(s) 1659
MflI RGATCY 2 cut(s) 1025, 2049
MhlI GDGCHC 5 cut(s) 210, 1343, 1386, 2090, 2250
MluCI AATT 9 cut(s) 329, 379, 1294, 1311, 1484, 1650, 1659, 1988, 1994
MlyI GAGTC 4 cut(s) 591, 1038, 1072, 1188
MmeI TCCRAC 1 cut(s) 2216
MroXI GAANNNNTTC 1 cut(s) 183
MslI CAYNNNNRTG 2 cut(s) 1231, 2225
MspA1I CMGCKG 2 cut(s) 655, 1833
MspI CCGG 3 cut(s) 472, 964, 1282
MspR9I CCNGG 6 cut(s) 473, 752, 964, 965, 1283, 1784
MunI CAATTG 1 cut(s) 1659
Mva1269I GAATGC 1 cut(s) 672
MvaI CCWGG 2 cut(s) 752, 1784
MwoI GCNNNNNNNGC 4 cut(s) 652, 1038, 1400, 2085
NciI CCSGG 4 cut(s) 473, 964, 965, 1283
NdeI CATATG 1 cut(s) 16
NdeII GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
NlaIV GGNNCC 8 cut(s) 186, 209, 372, 391, 647, 928, 1328, 1329
NmuCI GTSAC 3 cut(s) 467, 1739, 1979
NspI RCATGY 2 cut(s) 1290, 1686
PaeI GCATGC 1 cut(s) 1290
PaeR7I CTCGAG 1 cut(s) 1336
PagI TCATGA 2 cut(s) 319, 510
PceI AGGCCT 1 cut(s) 1782
PciI ACATGT 1 cut(s) 1682
PcsI WCGNNNNNNNCGW 2 cut(s) 356, 1188
PctI GAATGC 1 cut(s) 672
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 1 cut(s) 457
PflMI CCANNNNNTGG 1 cut(s) 590
PkrI GCNGC 4 cut(s) 164, 654, 1835, 1918
Ple19I CGATCG 1 cut(s) 999
PleI GAGTC 4 cut(s) 591, 1038, 1071, 1187
PpsI GAGTC 4 cut(s) 591, 1038, 1071, 1187
Ppu21I YACGTR 2 cut(s) 30, 2197
PpuMI RGGWCCY 1 cut(s) 1327
PscI ACATGT 1 cut(s) 1682
Psp124BI GAGCTC 1 cut(s) 1386
Psp5II RGGWCCY 1 cut(s) 1327
Psp6I CCWGG 2 cut(s) 750, 1782
PspEI GGTNACC 1 cut(s) 1979
PspFI CCCAGC 1 cut(s) 2041
PspGI CCWGG 2 cut(s) 750, 1782
PspN4I GGNNCC 8 cut(s) 186, 209, 372, 391, 647, 928, 1328, 1329
PspPI GGNCC 6 cut(s) 370, 482, 926, 1327, 1625, 1710
PspPPI RGGWCCY 1 cut(s) 1327
PspXI VCTCGAGB 1 cut(s) 1336
PsrI GAACNNNNNNTAC 2 cut(s) 109, 141
PsuI RGATCY 2 cut(s) 1025, 2049
PvuI CGATCG 1 cut(s) 999
PvuII CAGCTG 1 cut(s) 1833
RsaI GTAC 4 cut(s) 554, 840, 1703, 2199
RsaNI GTAC 4 cut(s) 553, 839, 1702, 2198
RseI CAYNNNNRTG 2 cut(s) 1231, 2225
SacI GAGCTC 1 cut(s) 1386
SatI GCNGC 4 cut(s) 163, 653, 1834, 1917
Sau3AI GATC 7 cut(s) 195, 634, 996, 1025, 1568, 1572, 2049
Sau96I GGNCC 6 cut(s) 370, 482, 926, 1327, 1625, 1710
ScaI AGTACT 1 cut(s) 1703
SchI GAGTC 4 cut(s) 591, 1038, 1072, 1188
ScrFI CCNGG 6 cut(s) 473, 752, 964, 965, 1283, 1784
SduI GDGCHC 5 cut(s) 210, 1343, 1386, 2090, 2250
SfaNI GCATC 1 cut(s) 2197
Sfr274I CTCGAG 1 cut(s) 1336
SinI GGWCC 4 cut(s) 370, 482, 926, 1327
SlaI CTCGAG 1 cut(s) 1336
SmaI CCCGGG 1 cut(s) 965
SmiMI CAYNNNNRTG 2 cut(s) 1231, 2225
SmlI CTYRAG 1 cut(s) 1336
SmoI CTYRAG 1 cut(s) 1336
SpeI ACTAGT 1 cut(s) 809
SphI GCATGC 1 cut(s) 1290
Sse9I AATT 9 cut(s) 329, 379, 1294, 1311, 1484, 1650, 1659, 1988, 1994
SseBI AGGCCT 1 cut(s) 1782
SsiI CCGC 5 cut(s) 158, 650, 653, 1916, 2216
SspI AATATT 1 cut(s) 1819
SspMI CTAG 5 cut(s) 47, 810, 858, 1029, 1760
SstI GAGCTC 1 cut(s) 1386
StuI AGGCCT 1 cut(s) 1782
StyD4I CCNGG 6 cut(s) 471, 750, 962, 963, 1281, 1782
StyI CCWWGG 1 cut(s) 1353
TaaI ACNGT 6 cut(s) 482, 838, 1201, 1378, 1846, 1883
TaiI ACGT 4 cut(s) 32, 1463, 2128, 2199
TaqI TCGA 8 cut(s) 350, 999, 1337, 1380, 1571, 1734, 2016, 2023
TasI AATT 9 cut(s) 329, 379, 1294, 1311, 1484, 1650, 1659, 1988, 1994
TatI WGTACW 1 cut(s) 1701
TauI GCSGC 2 cut(s) 655, 1919
TfiI GAWTC 1 cut(s) 457
TscAI CASTG 6 cut(s) 890, 1206, 1323, 1886, 1903, 2187
TseFI GTSAC 3 cut(s) 467, 1739, 1979
TseI GCWGC 2 cut(s) 162, 1833
Tsp45I GTSAC 3 cut(s) 467, 1739, 1979
TspGWI ACGGA 1 cut(s) 1180
TspMI CCCGGG 1 cut(s) 963
TspRI CASTG 6 cut(s) 890, 1206, 1323, 1886, 1903, 2187
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 370, 482, 926, 1327
XagI CCTNNNNNAGG 2 cut(s) 636, 784
XapI RAATTY 1 cut(s) 1311
XceI RCATGY 2 cut(s) 1290, 1686
XcmI CCANNNNNNNNNTGG 1 cut(s) 218
XhoI CTCGAG 1 cut(s) 1336
XmaI CCCGGG 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 5 cut(s) 47, 810, 858, 1029, 1760
ZrmI AGTACT 1 cut(s) 1703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.