pycom12g09860

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
11708393 .. 11712587
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGTCGGTTTCTGCCAAGGGGATGGCGATAACCGGGATCGAAGATAGGCGATACTGGAACTGGATTCCTACTGAAGAATCTAGGTTTCAGGTTGTGGCGTATTTACAGCAAATATGGTGGTTTGAAGTAGATGGGGTGGTGAAGTTCCCGTTTCCTGCTGATATCTACACTTTGTCTTTCAGGCTTCACATTGGAAGATTCTCAAAAAGATTGGGACGACGTGTCTGCAATTTTGAGCACACCCACGGTTGGGATTTAAAGCCCGTGCGATATGAGTTGTCGACTTCAGATGGACAGCAAGCATCGTATGAATGCTGCTTGGATGAAATTGAACATGAGGATGCATATGGAAACCATAAGCGCGGATGCTGGGTTGAGTACAAGGTTGGTGAATTTACTGTCACCGATACAGATCCAGTAACAGAAGTTAAGTTTTCCATGAAACAGATAGATTGCACACATTCGAAAGGTGGGCTATGCGTGGATTCTGTGTCTATCGTGCCCAGTGATTTGAAAGAACGTAGAAGAGGGGTCTGGAAGGCGATTTCAGGTGGATTGAAGCTCCAACTAGAAGACACCACGGTTGAAACACCTCGCCATCTCGATCAATATGTGGGAATTTTAATAAAGGTCAGTTTTGATTGGAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2

Protein Analysis

218

Amino Acids

25.08

Weight (kDa)

5.99

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 2 - 168 1.1e-38 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 281
AccII CGCG 1 cut(s) 363
AciI CCGC 1 cut(s) 363
AclWI GGATC 2 cut(s) 44, 407
AcsI RAATTY 2 cut(s) 392, 618
AcuI CTGAAG 2 cut(s) 93, 270
AfaI GTAC 1 cut(s) 380
AfiI CCNNNNNNNGG 2 cut(s) 249, 250
AflIII ACRYGT 1 cut(s) 220
AgsI TTSAA 5 cut(s) 125, 332, 514, 559, 587
AhdI GACNNNNNGTC 1 cut(s) 221
AjiI CACGTC 1 cut(s) 221
AluBI AGCT 1 cut(s) 562
AluI AGCT 1 cut(s) 562
Alw21I GWGCWC 1 cut(s) 240
AlwI GGATC 2 cut(s) 44, 407
ApeKI GCWGC 1 cut(s) 315
ApoI RAATTY 2 cut(s) 392, 618
AspLEI GCGC 1 cut(s) 363
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 3 cut(s) 151, 394, 401
AsuII TTCGAA 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 579
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 1 cut(s) 302
BccI CCATC 4 cut(s) 16, 125, 284, 606
BcgI CGANNNNNNTGC 2 cut(s) 207, 241
BcnI CCSGG 1 cut(s) 34
BfaI CTAG 2 cut(s) 81, 569
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 34
BmeRI GACNNNNNGTC 1 cut(s) 221
BmgBI CACGTC 1 cut(s) 221
BmrFI CCNGG 1 cut(s) 34
BmrI ACTGGG 1 cut(s) 498
BmsI GCATC 3 cut(s) 311, 331, 356
BmuI ACTGGG 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 34
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 3 cut(s) 15, 244, 579
Bsc4I CCNNNNNNNGG 2 cut(s) 249, 250
Bse1I ACTGG 4 cut(s) 59, 65, 416, 504
Bse8I GATNNNNATC 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 15, 244, 579
BseGI GGATG 4 cut(s) 27, 328, 346, 371
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 249, 250
BseNI ACTGG 4 cut(s) 59, 65, 416, 504
BseSI GKGCMC 1 cut(s) 504
BseXI GCAGC 1 cut(s) 302
BseYI CCCAGC 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 228
BslI CCNNNNNNNGG 2 cut(s) 249, 250
BsmFI GGGAC 1 cut(s) 228
BsmI GAATGC 1 cut(s) 317
Bsp119I TTCGAA 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 240, 504
Bsp143I GATC 3 cut(s) 36, 412, 604
BspACI CCGC 1 cut(s) 363
BspFNI CGCG 1 cut(s) 363
BspHI TCATGA 1 cut(s) 650
BspPI GGATC 2 cut(s) 44, 407
BspT104I TTCGAA 1 cut(s) 464
BsrI ACTGG 4 cut(s) 59, 65, 416, 504
BssECI CCNNGG 3 cut(s) 15, 244, 579
BssMI GATC 3 cut(s) 36, 412, 604
BssT1I CCWWGG 1 cut(s) 15
Bst4CI ACNGT 3 cut(s) 248, 400, 583
Bst6I CTCTTC 1 cut(s) 520
BstBI TTCGAA 1 cut(s) 464
BstC8I GCNNGC 1 cut(s) 300
BstDSI CCRYGG 2 cut(s) 244, 579
BstF5I GGATG 4 cut(s) 27, 328, 346, 371
BstFNI CGCG 1 cut(s) 363
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 3 cut(s) 39, 415, 607
BstMBI GATC 3 cut(s) 36, 412, 604
BstSCI CCNGG 1 cut(s) 32
BstSLI GKGCMC 1 cut(s) 504
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 302
BstV2I GAAGAC 1 cut(s) 579
BstX2I RGATCY 1 cut(s) 412
BstXI CCANNNNNNTGG 1 cut(s) 22
BstYI RGATCY 1 cut(s) 412
BtgI CCRYGG 2 cut(s) 244, 579
BtrI CACGTC 1 cut(s) 221
BtsCI GGATG 4 cut(s) 27, 328, 346, 371
BtsIMutI CAGTG 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 300
CciI TCATGA 1 cut(s) 650
CfoI GCGC 1 cut(s) 363
Csp6I GTAC 1 cut(s) 379
CspCI CAANNNNNGTGG 2 cut(s) 98, 133
CviAII CATG 3 cut(s) 335, 439, 651
CviJI RGCY 4 cut(s) 184, 262, 475, 562
CviKI_1 RGCY 4 cut(s) 184, 262, 475, 562
CviQI GTAC 1 cut(s) 379
DpnI GATC 3 cut(s) 38, 414, 606
DpnII GATC 3 cut(s) 36, 412, 604
DraI TTTAAA 1 cut(s) 258
DriI GACNNNNNGTC 1 cut(s) 221
Eam1104I CTCTTC 1 cut(s) 520
Eam1105I GACNNNNNGTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 520
Eco130I CCWWGG 1 cut(s) 15
Eco32I GATATC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 93, 270
EcoRV GATATC 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 15
FaeI CATG 3 cut(s) 338, 442, 654
FaqI GGGAC 1 cut(s) 228
FatI CATG 3 cut(s) 334, 438, 650
FauNDI CATATG 1 cut(s) 346
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 4 cut(s) 34, 335, 353, 378
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 2 cut(s) 81, 569
GlaI GCGC 1 cut(s) 362
GluI GCNGC 1 cut(s) 316
GsaI CCCAGC 1 cut(s) 373
HapII CCGG 1 cut(s) 33
HhaI GCGC 1 cut(s) 363
Hin1II CATG 3 cut(s) 338, 442, 654
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HincII GTYRAC 1 cut(s) 282
HindII GTYRAC 1 cut(s) 282
HinfI GANTC 4 cut(s) 64, 77, 198, 485
HpaII CCGG 1 cut(s) 33
HphI GGTGA 3 cut(s) 151, 394, 401
Hpy166II GTNNAC 1 cut(s) 282
Hpy188I TCNGA 1 cut(s) 289
Hpy188III TCNNGA 3 cut(s) 535, 602, 651
Hpy8I GTNNAC 1 cut(s) 282
Hpy99I CGWCG 1 cut(s) 222
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 248, 400, 583
HpyCH4IV ACGT 2 cut(s) 220, 520
HpyCH4V TGCA 3 cut(s) 228, 344, 456
HpySE526I ACGT 2 cut(s) 220, 520
Hsp92II CATG 3 cut(s) 338, 442, 654
HspAI GCGC 1 cut(s) 361
Kzo9I GATC 3 cut(s) 36, 412, 604
LmnI GCTCC 1 cut(s) 567
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 3 cut(s) 311, 331, 356
MaeI CTAG 2 cut(s) 81, 569
MaeII ACGT 2 cut(s) 220, 520
MaeIII GTNAC 2 cut(s) 400, 418
MalI GATC 3 cut(s) 38, 414, 606
MboI GATC 3 cut(s) 36, 412, 604
MboII GAAGA 5 cut(s) 53, 86, 207, 537, 584
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 240, 504
MluCI AATT 4 cut(s) 229, 327, 392, 618
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 3 cut(s) 331, 521, 603
Mph1103I ATGCAT 1 cut(s) 346
MseI TTAA 3 cut(s) 257, 429, 623
MslI CAYNNNNRTG 1 cut(s) 339
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 34
Mva1269I GAATGC 1 cut(s) 317
MvnI CGCG 1 cut(s) 363
NciI CCSGG 1 cut(s) 34
NdeI CATATG 1 cut(s) 346
NdeII GATC 3 cut(s) 36, 412, 604
NlaIII CATG 3 cut(s) 338, 442, 654
NmuCI GTSAC 1 cut(s) 400
NsiI ATGCAT 1 cut(s) 346
NspV TTCGAA 1 cut(s) 464
PagI TCATGA 1 cut(s) 650
PctI GAATGC 1 cut(s) 317
PfeI GAWTC 4 cut(s) 64, 77, 198, 485
PkrI GCNGC 1 cut(s) 317
PspFI CCCAGC 1 cut(s) 369
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 1 cut(s) 380
RsaNI GTAC 1 cut(s) 379
RseI CAYNNNNRTG 1 cut(s) 339
SalI GTCGAC 1 cut(s) 280
SaqAI TTAA 3 cut(s) 257, 429, 623
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 3 cut(s) 36, 412, 604
ScrFI CCNGG 1 cut(s) 34
SduI GDGCHC 2 cut(s) 240, 504
SfaNI GCATC 3 cut(s) 311, 331, 356
SfuI TTCGAA 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 339
Sse9I AATT 4 cut(s) 229, 327, 392, 618
SsiI CCGC 1 cut(s) 363
SspMI CTAG 2 cut(s) 81, 569
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 15
TaaI ACNGT 3 cut(s) 248, 400, 583
TaiI ACGT 2 cut(s) 223, 523
TaqI TCGA 4 cut(s) 39, 281, 464, 603
TasI AATT 4 cut(s) 229, 327, 392, 618
TatI WGTACW 1 cut(s) 378
TfiI GAWTC 4 cut(s) 64, 77, 198, 485
Tru1I TTAA 3 cut(s) 257, 429, 623
Tru9I TTAA 3 cut(s) 257, 429, 623
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 1 cut(s) 315
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 4 cut(s) 324, 339, 455, 639
TspRI CASTG 1 cut(s) 511
XapI RAATTY 2 cut(s) 392, 618
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 2 cut(s) 81, 569
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.