pycom12g10460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
12457638 .. 12458542
905 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGAAGCTCTTCGTTGATTTTGATCATGGTGGCCGGAGTGAGGAGCTTGAGGTTTGTTGGCAGCGTGAGGAGACAGAGGAAGAAGCTCCACCATCACCATTGAGCACAGCCTCACAGACCGCCGATTTTCCACGACCTTCGATCCAGTTCACCGCAGCGGGCTTCCTTCAGAGGGAAAGGAGAAGCTGGAGGAATTGCAGCACAAATATGAGTGTACAAATTCCGGCGGCAGTGGAGTGCCACCCAAAATTCACCATTTTCGTTAGTTCTGACATTGTCTGGAAATCCCGGCAGCACTTGAAGTTGCCAGACCACCTTCTGGCCCCACCTTCAACAGACCAAGATATGCATCTGCGATGTATAAATCACCTGTTTTCTTGTCAAAGCGTAGCCCCAGAGGCCTTCCGCAGATGTGCTCATTCTTCAAGTAACTCAAAGGTGATGGCTTTGGGCTACATATATCTGACCTGTGAGGCGAAGTATAGGCGAAATCAGTCCAAGCTTGGCCATTCCAGAACAAGACTCTGCCATCAGCAACGCCGATGTAAGGTCCGCGGCCAAGCGGGTCAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

192

Amino Acids

21.93

Weight (kDa)

9.19

Isoelectric Point (pI)

73.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 319
AccII CGCG 1 cut(s) 555
AciI CCGC 8 cut(s) 120, 153, 158, 227, 406, 553, 555, 563
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 3 cut(s) 31, 505, 556
AcsI RAATTY 2 cut(s) 219, 248
AcuI CTGAAG 1 cut(s) 152
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 4 cut(s) 40, 172, 319, 547
AgsI TTSAA 3 cut(s) 301, 333, 426
AluBI AGCT 5 cut(s) 7, 46, 86, 186, 502
AluI AGCT 5 cut(s) 7, 46, 86, 186, 502
Alw21I GWGCWC 2 cut(s) 107, 418
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 136
AoxI GGCC 5 cut(s) 31, 321, 399, 505, 556
ApeKI GCWGC 4 cut(s) 61, 155, 198, 292
ApoI RAATTY 2 cut(s) 219, 248
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 322, 550
AsuC2I CCSGG 1 cut(s) 289
AsuHPI GGTGA 5 cut(s) 87, 142, 244, 359, 451
AvaII GGWCC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 507
Bbv12I GWGCWC 2 cut(s) 107, 418
BbvI GCAGC 4 cut(s) 73, 167, 210, 304
BccI CCATC 3 cut(s) 100, 436, 537
BclI TGATCA 1 cut(s) 22
BcnI CCSGG 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 65
BglI GCCNNNNNGGC 1 cut(s) 398
BisI GCNGC 6 cut(s) 62, 156, 199, 228, 293, 556
BlsI GCNGC 6 cut(s) 63, 157, 200, 229, 294, 557
Bme1390I CCNGG 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 2 cut(s) 322, 550
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 358
BpmI CTGGAG 1 cut(s) 208
BpuEI CTTGAG 1 cut(s) 68
BpuMI CCSGG 1 cut(s) 289
BsaBI GATNNNNATC 2 cut(s) 21, 348
BsaJI CCNNGG 1 cut(s) 553
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 172, 319, 547
Bse1I ACTGG 1 cut(s) 145
Bse8I GATNNNNATC 2 cut(s) 21, 348
BseDI CCNNGG 1 cut(s) 553
BseJI GATNNNNATC 2 cut(s) 21, 348
BseLI CCNNNNNNNGG 4 cut(s) 40, 172, 319, 547
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 56, 83
BseXI GCAGC 4 cut(s) 73, 167, 210, 304
Bsh1236I CGCG 1 cut(s) 555
BshFI GGCC 5 cut(s) 33, 323, 401, 507, 558
BsiHKAI GWGCWC 2 cut(s) 107, 418
BsiSI CCGG 3 cut(s) 34, 224, 289
BslI CCNNNNNNNGG 4 cut(s) 40, 172, 319, 547
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 5 cut(s) 33, 323, 401, 507, 558
Bsp1286I GDGCHC 2 cut(s) 107, 418
Bsp1407I TGTACA 1 cut(s) 214
Bsp143I GATC 2 cut(s) 22, 141
BspACI CCGC 8 cut(s) 120, 153, 158, 227, 406, 553, 555, 563
BspANI GGCC 5 cut(s) 33, 323, 401, 507, 558
BspFNI CGCG 1 cut(s) 555
BspLI GGNNCC 1 cut(s) 324
BspPI GGATC 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 14
BsrGI TGTACA 1 cut(s) 214
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 553
BssMI GATC 2 cut(s) 22, 141
Bst6I CTCTTC 1 cut(s) 14
BstAUI TGTACA 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 160
BstDSI CCRYGG 1 cut(s) 553
BstFNI CGCG 1 cut(s) 555
BstKTI GATC 2 cut(s) 25, 144
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 2 cut(s) 22, 141
BstMWI GCNNNNNNNGC 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 287
BstUI CGCG 1 cut(s) 555
BstV1I GCAGC 4 cut(s) 73, 167, 210, 304
BsuRI GGCC 5 cut(s) 33, 323, 401, 507, 558
BtgI CCRYGG 1 cut(s) 553
BtgZI GCGATG 1 cut(s) 370
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 322, 550
Cfr42I CCGCGG 1 cut(s) 556
Csp6I GTAC 1 cut(s) 215
CviAII CATG 1 cut(s) 26
CviQI GTAC 1 cut(s) 215
DpnI GATC 2 cut(s) 24, 143
DpnII GATC 2 cut(s) 22, 141
EaeI YGGCCR 3 cut(s) 31, 505, 556
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco147I AGGCCT 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 152
EcoT22I ATGCAT 1 cut(s) 351
FaeI CATG 1 cut(s) 29
FaiI YATR 7 cut(s) 27, 209, 347, 362, 458, 460, 483
FatI CATG 1 cut(s) 25
FauI CCCGC 2 cut(s) 151, 556
FbaI TGATCA 1 cut(s) 22
Fnu4HI GCNGC 6 cut(s) 62, 156, 199, 228, 293, 556
Fsp4HI GCNGC 6 cut(s) 62, 156, 199, 228, 293, 556
GluI GCNGC 6 cut(s) 62, 156, 199, 228, 293, 556
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 5 cut(s) 33, 323, 401, 507, 558
HapII CCGG 3 cut(s) 34, 224, 289
Hin1II CATG 1 cut(s) 29
HindIII AAGCTT 1 cut(s) 500
HinfI GANTC 1 cut(s) 522
HpaII CCGG 3 cut(s) 34, 224, 289
HphI GGTGA 5 cut(s) 87, 142, 244, 359, 451
Hpy166II GTNNAC 2 cut(s) 150, 215
Hpy188I TCNGA 3 cut(s) 171, 271, 465
Hpy188III TCNNGA 2 cut(s) 280, 513
Hpy8I GTNNAC 2 cut(s) 150, 215
HpyAV CCTTC 5 cut(s) 147, 176, 326, 339, 412
HpyCH4V TGCA 2 cut(s) 198, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 398
Hsp92II CATG 1 cut(s) 29
Ksp22I TGATCA 1 cut(s) 22
KspI CCGCGG 1 cut(s) 556
Kzo9I GATC 2 cut(s) 22, 141
LguI GCTCTTC 1 cut(s) 14
LmnI GCTCC 2 cut(s) 43, 91
Lsp1109I GCAGC 4 cut(s) 73, 167, 210, 304
LweI GCATC 1 cut(s) 358
MaeIII GTNAC 1 cut(s) 428
MalI GATC 2 cut(s) 24, 143
MboI GATC 2 cut(s) 22, 141
MboII GAAGA 2 cut(s) 92, 414
MhlI GDGCHC 2 cut(s) 107, 418
MlsI TGGCCA 1 cut(s) 507
MluCI AATT 3 cut(s) 193, 219, 248
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 1 cut(s) 516
MnlI CCTC 9 cut(s) 34, 43, 61, 70, 121, 165, 183, 391, 466
Mox20I TGGCCA 1 cut(s) 507
Mph1103I ATGCAT 1 cut(s) 351
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 507
MspA1I CMGCKG 2 cut(s) 158, 555
MspI CCGG 3 cut(s) 34, 224, 289
MspR9I CCNGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 555
MwoI GCNNNNNNNGC 1 cut(s) 398
NciI CCSGG 1 cut(s) 289
NdeII GATC 2 cut(s) 22, 141
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 1 cut(s) 324
NsiI ATGCAT 1 cut(s) 351
PceI AGGCCT 1 cut(s) 401
PciSI GCTCTTC 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 6 cut(s) 63, 157, 200, 229, 294, 557
PleI GAGTC 1 cut(s) 516
PpsI GAGTC 1 cut(s) 516
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 2 cut(s) 322, 550
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
RseI CAYNNNNRTG 1 cut(s) 206
SacII CCGCGG 1 cut(s) 556
SapI GCTCTTC 1 cut(s) 14
SatI GCNGC 6 cut(s) 62, 156, 199, 228, 293, 556
Sau3AI GATC 2 cut(s) 22, 141
Sau96I GGNCC 2 cut(s) 322, 550
SchI GAGTC 1 cut(s) 516
ScrFI CCNGG 1 cut(s) 289
SduI GDGCHC 2 cut(s) 107, 418
SfaNI GCATC 1 cut(s) 358
Sfr303I CCGCGG 1 cut(s) 556
SgrBI CCGCGG 1 cut(s) 556
SinI GGWCC 1 cut(s) 550
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 3 cut(s) 193, 219, 248
SseBI AGGCCT 1 cut(s) 401
SsiI CCGC 8 cut(s) 120, 153, 158, 227, 406, 553, 555, 563
StuI AGGCCT 1 cut(s) 401
StyD4I CCNGG 1 cut(s) 287
TaqI TCGA 1 cut(s) 140
TasI AATT 3 cut(s) 193, 219, 248
TatI WGTACW 1 cut(s) 214
TauI GCSGC 2 cut(s) 230, 558
TscAI CASTG 1 cut(s) 237
TseI GCWGC 4 cut(s) 61, 155, 198, 292
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 237
Tth111I GACNNNGTC 1 cut(s) 275
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 2 cut(s) 219, 248
XmnI GAANNNNTTC 1 cut(s) 8
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.