pycom12g11760

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
14167160 .. 14168386
1227 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGGCAAAGACATTGAAATTAGAGGTGGGTACTCGGCCAAGGACTACCAGGACCCTCCACCGGCGCTATTGATTGATGCACAGGAGCTAAGGAAGTGGTCGTTCTACAGAGCCATCATAGCTGAGTTCATTGCCACCCTCTTGTTCTTGTACGTCATTGTGCTCACTGTGATTGGATACAAGAGTCAGACTAACGCTTCCCTAAACGGAGACCAATGTGGTGGTGTTGGCATTCTTGGTATTGCTTGGGCCTTTGTTGGCATGATCTTTGTCCTTGTTTACTGCACTGCTGGCATCTCTGGTGGGTTTCATCTCAATCTTAAATGTGCATTGTTATGTGTAGGGGGGCACATAAACCTTGCAGTCACATTCGGGCTGTTTTTGGCAAGGAAAGTATCACTAGTGAGAGCCATATTGTACATGGCGGCTCAGTCATTGGGAGTCATATGTGGTGTTGGGCTAGTGAAAGCTTTCCAAAGTTCTTACTATACCAAGTATGGCGGTGGAGCCAATGAGCTTGCCGATGGCTACAACACAGACACTGGATTGGGTGCTGAGATTGTTGGCACCTTTTGTTCTTGTCTACACTGTCTTCTCTGCCACTTAACAGATCGATCCCAAGAGAAATGCAAGAGACTCCCATGTCCCTGTATTGGCACCCCTGCCAATTGGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

226

Amino Acids

24.15

Weight (kDa)

8.23

Isoelectric Point (pI)

34.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 29 - 106 2.7e-16 Major intrinsic protein
MIP PF00230 109 - 207 1.3e-20 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 642
AccB1I GGYRCC 2 cut(s) 566, 656
AccI GTMKAC 1 cut(s) 583
AciI CCGC 2 cut(s) 425, 501
AclWI GGATC 1 cut(s) 609
AcoI YGGCCR 1 cut(s) 36
AfaI GTAC 3 cut(s) 32, 152, 419
AfiI CCNNNNNNNGG 2 cut(s) 61, 653
AgsI TTSAA 1 cut(s) 17
AhlI ACTAGT 1 cut(s) 400
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 4 cut(s) 88, 122, 470, 517
AluI AGCT 4 cut(s) 88, 122, 470, 517
Alw21I GWGCWC 1 cut(s) 165
Alw26I GTCTC 2 cut(s) 204, 628
AlwI GGATC 1 cut(s) 609
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 2 cut(s) 36, 249
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 2 cut(s) 52, 249
AvaII GGWCC 1 cut(s) 52
BaeGI GKGCMC 1 cut(s) 351
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BanI GGYRCC 2 cut(s) 566, 656
BbsI GAAGAC 1 cut(s) 584
Bbv12I GWGCWC 1 cut(s) 165
BccI CCATC 2 cut(s) 122, 518
BciT130I CCWGG 1 cut(s) 50
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 204, 628
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 2 cut(s) 401, 461
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 68
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 1 cut(s) 426
BlsI GCNGC 1 cut(s) 427
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 249
BmiI GGNNCC 4 cut(s) 54, 508, 568, 658
BmrFI CCNGG 1 cut(s) 50
BmsI GCATC 2 cut(s) 67, 303
BpiI GAAGAC 1 cut(s) 584
Bpu10I CCTNAGC 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 613
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 653
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 547
Bse3DI GCAATG 1 cut(s) 129
BseBI CCWGG 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 613
BseDI CCNNGG 1 cut(s) 39
BseLI CCNNNNNNNGG 2 cut(s) 61, 653
BseMI GCAATG 1 cut(s) 129
BseMII CTCAG 3 cut(s) 114, 443, 546
BseNI ACTGG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 351
BsgI GTGCAG 1 cut(s) 268
BshFI GGCC 2 cut(s) 38, 251
BshNI GGYRCC 2 cut(s) 566, 656
BshVI ATCGAT 1 cut(s) 613
BsiHKAI GWGCWC 1 cut(s) 165
BsiSI CCGG 1 cut(s) 62
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 61, 653
BsmAI GTCTC 2 cut(s) 204, 628
BsmFI GGGAC 1 cut(s) 630
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 2 cut(s) 38, 251
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 165, 351
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 3 cut(s) 264, 610, 614
BspACI CCGC 2 cut(s) 425, 501
BspANI GGCC 2 cut(s) 38, 251
BspCNI CTCAG 3 cut(s) 115, 442, 547
BspDI ATCGAT 1 cut(s) 613
BspLI GGNNCC 4 cut(s) 54, 508, 568, 658
BspPI GGATC 1 cut(s) 609
BspT107I GGYRCC 2 cut(s) 566, 656
BspTNI GGTCTC 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 129
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 1 cut(s) 547
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 3 cut(s) 264, 610, 614
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 2 cut(s) 169, 590
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 292, 519
BstDEI CTNAG 4 cut(s) 89, 123, 429, 555
BstH2I RGCGCY 1 cut(s) 68
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 3 cut(s) 267, 613, 617
BstMAI GTCTC 2 cut(s) 204, 628
BstMBI GATC 3 cut(s) 264, 610, 614
BstMWI GCNNNNNNNGC 2 cut(s) 119, 291
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 351
BstV2I GAAGAC 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 221
Bsu15I ATCGAT 1 cut(s) 613
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 38, 251
BsuTUI ATCGAT 1 cut(s) 613
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 4 cut(s) 165, 285, 540, 586
Cac8I GCNNGC 2 cut(s) 292, 519
CaiI CAGNNNCTG 1 cut(s) 542
CfoI GCGC 1 cut(s) 67
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 52, 249
ClaI ATCGAT 1 cut(s) 613
Csp6I GTAC 3 cut(s) 31, 151, 418
CviAII CATG 3 cut(s) 262, 421, 642
CviQI GTAC 3 cut(s) 31, 151, 418
DdeI CTNAG 4 cut(s) 89, 123, 429, 555
DpnI GATC 3 cut(s) 266, 612, 616
DpnII GATC 3 cut(s) 264, 610, 614
DrdI GACNNNNNNGTC 1 cut(s) 642
DseDI GACNNNNNNGTC 1 cut(s) 642
EaeI YGGCCR 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 39
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 1 cut(s) 52
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 3 cut(s) 265, 424, 645
FaqI GGGAC 1 cut(s) 630
FatI CATG 3 cut(s) 261, 420, 641
FauNDI CATATG 1 cut(s) 446
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 426
Fsp4HI GCNGC 1 cut(s) 426
FspBI CTAG 2 cut(s) 401, 461
GlaI GCGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 68
HaeIII GGCC 2 cut(s) 38, 251
HapII CCGG 1 cut(s) 62
HhaI GCGC 1 cut(s) 67
Hin1II CATG 3 cut(s) 265, 424, 645
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 468
HinfI GANTC 3 cut(s) 184, 441, 636
HpaII CCGG 1 cut(s) 62
Hpy166II GTNNAC 2 cut(s) 280, 584
Hpy188I TCNGA 1 cut(s) 189
Hpy8I GTNNAC 2 cut(s) 280, 584
HpyCH4III ACNGT 2 cut(s) 169, 590
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 5 cut(s) 80, 285, 329, 362, 630
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 291
HpyF3I CTNAG 4 cut(s) 89, 123, 429, 555
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 3 cut(s) 265, 424, 645
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 3 cut(s) 264, 610, 614
LmnI GCTCC 2 cut(s) 85, 506
LpnPI CCDG 8 cut(s) 35, 62, 68, 75, 276, 285, 528, 661
LweI GCATC 2 cut(s) 67, 303
MaeI CTAG 2 cut(s) 401, 461
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 364
MalI GATC 3 cut(s) 266, 612, 616
MboI GATC 3 cut(s) 264, 610, 614
MboII GAAGA 1 cut(s) 584
MfeI CAATTG 1 cut(s) 667
MhlI GDGCHC 2 cut(s) 165, 351
MluCI AATT 2 cut(s) 18, 667
MlyI GAGTC 3 cut(s) 193, 450, 630
MnlI CCTC 3 cut(s) 17, 66, 149
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 2 cut(s) 321, 605
MslI CAYNNNNRTG 1 cut(s) 334
MspI CCGG 1 cut(s) 62
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 667
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 2 cut(s) 119, 291
NdeI CATATG 1 cut(s) 446
NdeII GATC 3 cut(s) 264, 610, 614
NlaIII CATG 3 cut(s) 265, 424, 645
NlaIV GGNNCC 4 cut(s) 54, 508, 568, 658
NmeAIII GCCGAG 1 cut(s) 14
NmuCI GTSAC 1 cut(s) 364
PctI GAATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 470
PkrI GCNGC 1 cut(s) 427
PleI GAGTC 3 cut(s) 192, 449, 630
PpsI GAGTC 3 cut(s) 192, 449, 630
PpuMI RGGWCCY 1 cut(s) 52
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 48
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 4 cut(s) 54, 508, 568, 658
PspPI GGNCC 2 cut(s) 52, 249
PspPPI RGGWCCY 1 cut(s) 52
PstNI CAGNNNCTG 1 cut(s) 542
RsaI GTAC 3 cut(s) 32, 152, 419
RsaNI GTAC 3 cut(s) 31, 151, 418
RseI CAYNNNNRTG 1 cut(s) 334
SaqAI TTAA 2 cut(s) 321, 605
SatI GCNGC 1 cut(s) 426
Sau3AI GATC 3 cut(s) 264, 610, 614
Sau96I GGNCC 2 cut(s) 52, 249
SchI GAGTC 3 cut(s) 193, 450, 630
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 2 cut(s) 165, 351
SetI ASST 8 cut(s) 28, 90, 124, 156, 360, 472, 519, 572
SfaNI GCATC 2 cut(s) 67, 303
SfcI CTRYAG 1 cut(s) 106
SgrAI CRCCGGYG 1 cut(s) 61
SinI GGWCC 1 cut(s) 52
SmiMI CAYNNNNRTG 1 cut(s) 334
SpeI ACTAGT 1 cut(s) 400
Sse9I AATT 2 cut(s) 18, 667
SsiI CCGC 2 cut(s) 425, 501
SspMI CTAG 2 cut(s) 401, 461
StyD4I CCNGG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 2 cut(s) 169, 590
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 613
TasI AATT 2 cut(s) 18, 667
TatI WGTACW 1 cut(s) 417
TauI GCSGC 1 cut(s) 428
Tru1I TTAA 2 cut(s) 321, 605
Tru9I TTAA 2 cut(s) 321, 605
TscAI CASTG 4 cut(s) 172, 292, 547, 593
TseFI GTSAC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 118, 299
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 4 cut(s) 172, 292, 547, 593
VpaK11BI GGWCC 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 2 cut(s) 401, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.