pycom12g12060

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
14545630 .. 14546705
1076 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGCTTGCAAACAAAGTTGCAAGCCAAGACCCAGATCCATTTGAAAAACAGGCGGCGAACGGAGGAGCTCACTCTGGGGGTCGAGTCCACTGTGTGAACCTGGTACACAGGCTCTACCCAACCATCGATCCAATCCTAGACCGTGACCATGCCAAATACCTCACAGGTCGGTGCCCGAGTCCGACCCTAGACCCAAAAGAAGTCCTCTACGCAAGGAACGACCGTGAAACACCCATGCTTCTCAACAACTATTATTACAAGAACCAGCTGAGCCACAAGTGTCTGCTCACGGTGGACCAAGAACTGGGCTCTGACCCATCGACAATGCCCTTTGTGGAAAATATGGCCGCTGACAACGGCTACTTCAGGGAGCAGCTTTCTAGGGCTGCCCTTCTTTTGTCTGAGAACAACCCTCTTACTGGGGACCAAGGAGAAGTGAGGAAGGCATGTCAATCTGTGAATGCAAGTTAA

Protein Analysis

157

Amino Acids

17.33

Weight (kDa)

5.92

Isoelectric Point (pI)

38.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 21 - 118 6.5e-17 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 171
AccB7I CCANNNNNTGG 1 cut(s) 304
AciI CCGC 2 cut(s) 53, 348
AclWI GGATC 2 cut(s) 29, 122
AcoI YGGCCR 1 cut(s) 345
AcuI CTGAAG 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 94
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 304, 419
AgsI TTSAA 1 cut(s) 44
AjnI CCWGG 1 cut(s) 99
AluBI AGCT 3 cut(s) 68, 268, 376
AluI AGCT 3 cut(s) 68, 268, 376
Alw21I GWGCWC 1 cut(s) 70
AlwI GGATC 2 cut(s) 29, 122
Ama87I CYCGRG 1 cut(s) 175
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 373, 386
AspS9I GGNCC 2 cut(s) 295, 424
AvaI CYCGRG 1 cut(s) 175
AvaII GGWCC 2 cut(s) 295, 424
BaeGI GKGCMC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 171
BanII GRGCYC 2 cut(s) 70, 311
Bbv12I GWGCWC 1 cut(s) 70
BbvI GCAGC 2 cut(s) 373, 385
BccI CCATC 2 cut(s) 131, 325
BceAI ACGGC 1 cut(s) 373
BciT130I CCWGG 1 cut(s) 101
BfaI CTAG 3 cut(s) 137, 188, 381
BisI GCNGC 4 cut(s) 54, 348, 374, 387
BlpI GCTNAGC 1 cut(s) 269
BlsI GCNGC 4 cut(s) 55, 349, 375, 388
Bme1390I CCNGG 1 cut(s) 101
Bme18I GGWCC 2 cut(s) 295, 424
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 295, 424
BmiI GGNNCC 2 cut(s) 173, 425
BmrFI CCNGG 1 cut(s) 101
BmrI ACTGGG 2 cut(s) 314, 429
BmuI ACTGGG 2 cut(s) 314, 429
Bpu1102I GCTNAGC 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 427
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 419
Bse1I ACTGG 2 cut(s) 309, 424
BseBI CCWGG 1 cut(s) 101
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 427
BseLI CCNNNNNNNGG 2 cut(s) 304, 419
BseMII CTCAG 2 cut(s) 260, 393
BseNI ACTGG 2 cut(s) 309, 424
BseRI GAGGAG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 176
BseXI GCAGC 2 cut(s) 373, 385
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 1 cut(s) 347
BshNI GGYRCC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 70
BsiHKCI CYCGRG 1 cut(s) 175
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 2 cut(s) 304, 419
BsmFI GGGAC 1 cut(s) 437
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 1 cut(s) 347
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 3 cut(s) 70, 176, 311
Bsp143I GATC 2 cut(s) 34, 127
Bsp1720I GCTNAGC 1 cut(s) 269
BspACI CCGC 2 cut(s) 53, 348
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 2 cut(s) 261, 394
BspDI ATCGAT 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 173, 425
BspPI GGATC 2 cut(s) 29, 122
BspT107I GGYRCC 1 cut(s) 171
BsrI ACTGG 2 cut(s) 309, 424
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 2 cut(s) 34, 127
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 4 cut(s) 92, 143, 224, 292
BstC8I GCNNGC 2 cut(s) 6, 22
BstDEI CTNAG 2 cut(s) 269, 402
BstKTI GATC 2 cut(s) 37, 130
BstMBI GATC 2 cut(s) 34, 127
BstMCI CGRYCG 1 cut(s) 223
BstNI CCWGG 1 cut(s) 101
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 176
BstV1I GCAGC 2 cut(s) 373, 385
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 347
BsuTUI ATCGAT 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 2 cut(s) 6, 22
Cfr13I GGNCC 2 cut(s) 295, 424
ClaI ATCGAT 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 99
Csp6I GTAC 1 cut(s) 104
CviAII CATG 3 cut(s) 149, 235, 447
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 2 cut(s) 269, 402
DpnI GATC 2 cut(s) 36, 129
DpnII GATC 2 cut(s) 34, 127
DraIII CACNNNGTG 1 cut(s) 94
EaeI YGGCCR 1 cut(s) 345
Ecl136II GAGCTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 427
Eco24I GRGCYC 2 cut(s) 70, 311
Eco47I GGWCC 2 cut(s) 295, 424
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 1 cut(s) 349
Eco88I CYCGRG 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 68
EcoRII CCWGG 1 cut(s) 99
EcoT14I CCWWGG 1 cut(s) 427
EcoT38I GRGCYC 2 cut(s) 70, 311
ErhI CCWWGG 1 cut(s) 427
FaeI CATG 3 cut(s) 152, 238, 450
FaiI YATR 4 cut(s) 150, 236, 344, 448
FaqI GGGAC 1 cut(s) 437
FatI CATG 3 cut(s) 148, 234, 446
Fnu4HI GCNGC 4 cut(s) 54, 348, 374, 387
FriOI GRGCYC 2 cut(s) 70, 311
Fsp4HI GCNGC 4 cut(s) 54, 348, 374, 387
FspBI CTAG 3 cut(s) 137, 188, 381
GluI GCNGC 4 cut(s) 54, 348, 374, 387
HaeIII GGCC 1 cut(s) 347
Hin1II CATG 3 cut(s) 152, 238, 450
HinfI GANTC 2 cut(s) 84, 178
Hpy166II GTNNAC 4 cut(s) 88, 97, 106, 295
Hpy188I TCNGA 3 cut(s) 183, 313, 403
Hpy8I GTNNAC 4 cut(s) 88, 97, 106, 295
HpyAV CCTTC 2 cut(s) 401, 436
HpyCH4III ACNGT 4 cut(s) 92, 143, 224, 292
HpyCH4V TGCA 3 cut(s) 8, 20, 464
HpyF3I CTNAG 2 cut(s) 269, 402
Hsp92II CATG 3 cut(s) 152, 238, 450
Kzo9I GATC 2 cut(s) 34, 127
LmnI GCTCC 2 cut(s) 65, 370
Lsp1109I GCAGC 2 cut(s) 373, 385
MabI ACCWGGT 1 cut(s) 99
MaeI CTAG 3 cut(s) 137, 188, 381
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 36, 129
MboI GATC 2 cut(s) 34, 127
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 70, 176, 311
MlyI GAGTC 2 cut(s) 93, 187
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 5 cut(s) 56, 170, 215, 423, 432
MseI TTAA 1 cut(s) 469
MspA1I CMGCKG 2 cut(s) 268, 350
MspR9I CCNGG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 1 cut(s) 101
NdeII GATC 2 cut(s) 34, 127
NlaIII CATG 3 cut(s) 152, 238, 450
NlaIV GGNNCC 2 cut(s) 173, 425
NmuCI GTSAC 1 cut(s) 143
NspI RCATGY 1 cut(s) 450
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PctI GAATGC 1 cut(s) 466
PflMI CCANNNNNTGG 1 cut(s) 304
PkrI GCNGC 4 cut(s) 55, 349, 375, 388
PleI GAGTC 2 cut(s) 92, 186
PpsI GAGTC 2 cut(s) 92, 186
Psp124BI GAGCTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 99
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 173, 425
PspPI GGNCC 2 cut(s) 295, 424
PsuI RGATCY 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 268
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
SacI GAGCTC 1 cut(s) 70
SaqAI TTAA 1 cut(s) 469
SatI GCNGC 4 cut(s) 54, 348, 374, 387
Sau3AI GATC 2 cut(s) 34, 127
Sau96I GGNCC 2 cut(s) 295, 424
SchI GAGTC 2 cut(s) 93, 187
ScrFI CCNGG 1 cut(s) 101
SduI GDGCHC 3 cut(s) 70, 176, 311
SetI ASST 6 cut(s) 70, 102, 162, 169, 270, 378
SexAI ACCWGGT 1 cut(s) 99
SinI GGWCC 2 cut(s) 295, 424
SsiI CCGC 2 cut(s) 53, 348
SspMI CTAG 3 cut(s) 137, 188, 381
SstI GAGCTC 1 cut(s) 70
StyD4I CCNGG 1 cut(s) 99
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 4 cut(s) 92, 143, 224, 292
TaqI TCGA 3 cut(s) 82, 126, 320
TauI GCSGC 2 cut(s) 56, 350
Tru1I TTAA 1 cut(s) 469
Tru9I TTAA 1 cut(s) 469
TscAI CASTG 1 cut(s) 95
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 2 cut(s) 373, 386
Tsp45I GTSAC 1 cut(s) 143
TspGWI ACGGA 1 cut(s) 75
TspRI CASTG 1 cut(s) 95
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 2 cut(s) 295, 424
XceI RCATGY 1 cut(s) 450
XspI CTAG 3 cut(s) 137, 188, 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.