pycom12g14290

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
16853874 .. 16854458
585 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
ATGGACTGCAATGAAGGAGGAGGAGGAGGGTCGACGAAAACGGAGGAGCGGAGAGAAGCTCTGGTGTATACGTGGGGATATCTTCCCGGAGTATCGCCGGTGAAGTCTCCGATAGTGTCTCTGTCGCCGGTGCGGTTTCCTAATCGGATGGAGGGTGGATATTCGTGGAAGGACGTCTGCGGCGGCGGTTGCGGATTCGCCATGGCCATCTCAGAGTGTGGGAAGCTGATGACGTGGGGGTCGACGGACGATGAGGGGCAGAGCTACGTCATTTCTGGGAAGCACGGGGGGACTCCGGAGGCATATCCTCTCCCAACTGAAGCTTCAATACTCAAGGCTGCTGCTGGTTGGGCTCATTGCGTTGCTGTTACGGGTAGGTTTTTTCGCTCTTATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

133

Amino Acids

14.04

Weight (kDa)

6.09

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 42 - 124 8.8e-07 RCC1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 238
AatII GACGTC 1 cut(s) 177
AccBSI CCGCTC 1 cut(s) 49
AccI GTMKAC 3 cut(s) 32, 68, 242
AccIII TCCGGA 1 cut(s) 295
AciI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
AcoI YGGCCR 1 cut(s) 204
AcuI CTGAAG 1 cut(s) 339
AcyI GRCGYC 1 cut(s) 174
AgsI TTSAA 1 cut(s) 327
AjiI CACGTC 1 cut(s) 234
AjuI GAANNNNNNNTTGG 2 cut(s) 307, 339
AluBI AGCT 4 cut(s) 59, 226, 264, 323
AluI AGCT 4 cut(s) 59, 226, 264, 323
Alw26I GTCTC 2 cut(s) 111, 123
Aor13HI TCCGGA 1 cut(s) 295
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 338, 341
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 112
BalI TGGCCA 1 cut(s) 206
BanII GRGCYC 1 cut(s) 355
BarI GAAGNNNNNNTAC 2 cut(s) 312, 344
BbvI GCAGC 2 cut(s) 325, 328
BccI CCATC 2 cut(s) 142, 215
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 111, 123
BisI GCNGC 4 cut(s) 181, 184, 339, 342
BlsI GCNGC 4 cut(s) 182, 185, 340, 343
Bme1390I CCNGG 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 43, 75
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 87
BsaAI YACGTR 1 cut(s) 72
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 295
Bse118I RCCGGY 2 cut(s) 97, 127
Bse3DI GCAATG 2 cut(s) 16, 355
BseAI TCCGGA 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 153
BseMI GCAATG 2 cut(s) 16, 355
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 4 cut(s) 33, 36, 39, 59
BseXI GCAGC 2 cut(s) 325, 328
BshFI GGCC 1 cut(s) 206
BsiSI CCGG 4 cut(s) 87, 98, 128, 296
BslFI GGGAC 1 cut(s) 304
BsmAI GTCTC 2 cut(s) 111, 123
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 355
Bsp13I TCCGGA 1 cut(s) 295
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 224
BspEI TCCGGA 1 cut(s) 295
BsrBI CCGCTC 1 cut(s) 49
BsrDI GCAATG 2 cut(s) 16, 355
BsrFI RCCGGY 2 cut(s) 97, 127
BssAI RCCGGY 2 cut(s) 97, 127
BssECI CCNNGG 1 cut(s) 201
BssNAI GTATAC 1 cut(s) 69
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 201
Bst1107I GTATAC 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 72
BstDEI CTNAG 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 153
BstMAI GTCTC 2 cut(s) 111, 123
BstMWI GCNNNNNNNGC 2 cut(s) 189, 350
BstSCI CCNGG 1 cut(s) 85
BstV1I GCAGC 2 cut(s) 325, 328
BstZ17I GTATAC 1 cut(s) 69
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 234
BtsCI GGATG 1 cut(s) 153
Cfr10I RCCGGY 2 cut(s) 97, 127
CviAII CATG 1 cut(s) 202
CviJI RGCY 7 cut(s) 59, 206, 226, 264, 323, 338, 353
CviKI_1 RGCY 7 cut(s) 59, 206, 226, 264, 323, 338, 353
DdeI CTNAG 1 cut(s) 211
DrdI GACNNNNNNGTC 1 cut(s) 238
DseDI GACNNNNNNGTC 1 cut(s) 238
EaeI YGGCCR 1 cut(s) 204
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 355
Eco32I GATATC 1 cut(s) 80
Eco57I CTGAAG 1 cut(s) 339
EcoRV GATATC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 1 cut(s) 205
FaiI YATR 3 cut(s) 69, 203, 304
FaqI GGGAC 1 cut(s) 304
FatI CATG 1 cut(s) 201
FblI GTMKAC 3 cut(s) 32, 68, 242
Fnu4HI GCNGC 4 cut(s) 181, 184, 339, 342
FokI GGATG 1 cut(s) 160
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 4 cut(s) 181, 184, 339, 342
GluI GCNGC 4 cut(s) 181, 184, 339, 342
HaeIII GGCC 1 cut(s) 206
HapII CCGG 4 cut(s) 87, 98, 128, 296
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 205
HincII GTYRAC 2 cut(s) 33, 243
HindII GTYRAC 2 cut(s) 33, 243
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 2 cut(s) 195, 292
HpaII CCGG 4 cut(s) 87, 98, 128, 296
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 3 cut(s) 33, 69, 243
Hpy188I TCNGA 4 cut(s) 111, 147, 214, 398
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 3 cut(s) 33, 69, 243
Hpy99I CGWCG 2 cut(s) 37, 247
HpyAV CCTTC 2 cut(s) 8, 163
HpyCH4IV ACGT 4 cut(s) 71, 174, 233, 267
HpyCH4V TGCA 1 cut(s) 9
HpyF10VI GCNNNNNNNGC 2 cut(s) 189, 350
HpyF3I CTNAG 1 cut(s) 211
HpySE526I ACGT 4 cut(s) 71, 174, 233, 267
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 205
Kpn2I TCCGGA 1 cut(s) 295
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 7 cut(s) 47, 100, 111, 141, 261, 309, 330
Lsp1109I GCAGC 2 cut(s) 325, 328
MaeII ACGT 4 cut(s) 71, 174, 233, 267
MaeIII GTNAC 1 cut(s) 367
MbiI CCGCTC 1 cut(s) 49
MboII GAAGA 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 355
MlsI TGGCCA 1 cut(s) 206
MluNI TGGCCA 1 cut(s) 206
MlyI GAGTC 1 cut(s) 286
MnlI CCTC 9 cut(s) 11, 14, 17, 20, 37, 145, 247, 292, 318
Mox20I TGGCCA 1 cut(s) 206
MroI TCCGGA 1 cut(s) 295
MscI TGGCCA 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 206
MspI CCGG 4 cut(s) 87, 98, 128, 296
MspR9I CCNGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 189, 350
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 201
NlaIII CATG 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PfeI GAWTC 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 4 cut(s) 182, 185, 340, 343
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
Ppu21I YACGTR 1 cut(s) 72
SalI GTCGAC 2 cut(s) 31, 241
SatI GCNGC 4 cut(s) 181, 184, 339, 342
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 355
SetI ASST 9 cut(s) 61, 74, 177, 228, 236, 266, 270, 325, 380
SgrAI CRCCGGYG 2 cut(s) 97, 127
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SsiI CCGC 6 cut(s) 49, 133, 180, 183, 186, 192
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 1 cut(s) 201
TaiI ACGT 4 cut(s) 74, 177, 236, 270
TaqI TCGA 2 cut(s) 32, 242
TauI GCSGC 2 cut(s) 183, 186
TfiI GAWTC 1 cut(s) 195
TseI GCWGC 2 cut(s) 338, 341
TspDTI ATGAA 1 cut(s) 27
TspGWI ACGGA 2 cut(s) 56, 260
XmiI GTMKAC 3 cut(s) 32, 68, 242
ZraI GACGTC 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.