pycom12g14460

P5CS plays a key role in proline biosynthesis, leading to osmoregulation in plants

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
16979667 .. 16981523
1857 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 906 bp
ATGCCTTGTTTTGCAGCTTTGCTGATCTCCAAAATCCACAAAATGATTTTGATGGGAAGGCTTGTGCAGCTGTTGGACAGATATGTAGTTTCAGACTTGCCCCTCGTCACTTCAACAATTTTGGCTTTCTTTGAATTGCAGCTTGATGTGACTTCTTCGCAACTTCTTGTGACGGATAGCGTTTTTAGGGACGAAGCTTTCAGAGAGCAACTTTCTGAAACTGTGAAGTCGCTGTTAAAACTGAGGGTCATTCCAATCTTCAATGAAAATGACGCTGTTAGTACTAGGAAAGCTCCATACGAGGATTCTTCTGGCATATTTTGGGACAATGACAGTTTGGCCGGTCTATTGGCTCTGGAACTAAAAGCTGACCTTCTAGTTTTGTTAAGCGATGTAGAGGGCCTCTATAGCGGTCCTCCTACGGACCCAAAATCAAAGTTAATCCATACTTACGTGAAAGAGAAACATCAGGGAGAAATAACATTTGGCGACAAGTCAAGGGTGGGCAGAGGCGGTATGACCGCTAAAGTCAATGCTGCTGTGTGTGCAGCTTATGGTGGAATCCCTGTTGTTATCACTAGTGGCTATGACACAGACAACATAAAGAAAGTGCTTCGTGGAGACCGAATAGGCACTGTATTTCATCAAGATGCACATTTGTGGACTGTAGTTAAGGAAGAAGGTGCACGTGACATGGCTGTTGCAGCGCAGGAAGCATCTAGACAGTTCCGAGCTCTAGGATCTCAAGAGAGGAGGAAAATTTTGCTCGACGTAGCTGATGCCTTGGAGGCAAATGAAGGTGAAATTATAGCTGCGAATGAAGCTGATGTTGCTGCTGCTCAGGAGGGTATGCAGCTTAGCACTTGTGTAGTTGAAATTAAAACCTGCATCACTCCTGCTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004350 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00330 ko01100 ko01110 ko01230 map00330 map01100 map01110 map01230
Pfam Domains
Protein Families
AA_kinase Aldedh

Protein Analysis

302

Amino Acids

32.81

Weight (kDa)

5.17

Isoelectric Point (pI)

30.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AA_kinase PF00696 38 - 194 1.9e-21 Amino acid kinase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 893
AciI CCGC 3 cut(s) 411, 513, 522
AclWI GGATC 1 cut(s) 748
AcoI YGGCCR 1 cut(s) 339
AcsI RAATTY 1 cut(s) 759
AcvI CACGTG 1 cut(s) 689
AfaI GTAC 1 cut(s) 283
AgsI TTSAA 4 cut(s) 114, 134, 262, 875
AhlI ACTAGT 1 cut(s) 578
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AleI CACNNNNGTG 1 cut(s) 658
Alw21I GWGCWC 2 cut(s) 688, 736
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 684
AlwI GGATC 1 cut(s) 748
AoxI GGCC 2 cut(s) 339, 400
ApaLI GTGCAC 1 cut(s) 684
ApoI RAATTY 1 cut(s) 759
AspLEI GCGC 1 cut(s) 709
AspS9I GGNCC 3 cut(s) 400, 413, 424
AsuHPI GGTGA 1 cut(s) 812
AvaII GGWCC 2 cut(s) 413, 424
BaeGI GKGCMC 1 cut(s) 688
BanII GRGCYC 1 cut(s) 736
BbrPI CACGTG 1 cut(s) 689
Bbv12I GWGCWC 2 cut(s) 688, 736
BccI CCATC 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 615
BcuI ACTAGT 1 cut(s) 578
BfaI CTAG 5 cut(s) 285, 377, 579, 720, 737
BfmI CTRYAG 2 cut(s) 406, 666
BfuAI ACCTGC 1 cut(s) 893
BglI GCCNNNNNGGC 1 cut(s) 788
BlpI GCTNAGC 1 cut(s) 857
BmcAI AGTACT 1 cut(s) 283
Bme18I GGWCC 2 cut(s) 413, 424
BmgT120I GGNCC 3 cut(s) 400, 413, 424
BmiI GGNNCC 1 cut(s) 426
BmsI GCATC 4 cut(s) 640, 725, 769, 897
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 857
BpuEI CTTGAG 1 cut(s) 729
BsaAI YACGTR 2 cut(s) 454, 689
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 783
Bse118I RCCGGY 1 cut(s) 341
BseDI CCNNGG 1 cut(s) 783
BseMII CTCAG 2 cut(s) 233, 854
BseRI GAGGAG 1 cut(s) 766
BseSI GKGCMC 1 cut(s) 688
BsgI GTGCAG 2 cut(s) 86, 567
BshFI GGCC 2 cut(s) 341, 402
BsiHKAI GWGCWC 2 cut(s) 688, 736
BsiSI CCGG 1 cut(s) 342
BslFI GGGAC 2 cut(s) 203, 338
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 2 cut(s) 203, 338
BsnI GGCC 2 cut(s) 341, 402
Bso31I GGTCTC 1 cut(s) 615
Bsp1286I GDGCHC 2 cut(s) 688, 736
Bsp143I GATC 2 cut(s) 24, 740
Bsp1720I GCTNAGC 1 cut(s) 857
BspACI CCGC 3 cut(s) 411, 513, 522
BspANI GGCC 2 cut(s) 341, 402
BspCNI CTCAG 2 cut(s) 234, 853
BspLI GGNNCC 1 cut(s) 426
BspMI ACCTGC 1 cut(s) 893
BspPI GGATC 1 cut(s) 748
BspTNI GGTCTC 1 cut(s) 615
BsrFI RCCGGY 1 cut(s) 341
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 1 cut(s) 783
BssMI GATC 2 cut(s) 24, 740
BssT1I CCWWGG 1 cut(s) 783
Bst4CI ACNGT 5 cut(s) 223, 335, 637, 667, 726
BstBAI YACGTR 2 cut(s) 454, 689
BstDEI CTNAG 3 cut(s) 242, 840, 857
BstHHI GCGC 1 cut(s) 709
BstKTI GATC 2 cut(s) 27, 743
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 2 cut(s) 24, 740
BstMWI GCNNNNNNNGC 8 cut(s) 67, 408, 545, 704, 713, 788, 821, 830
BstSFI CTRYAG 2 cut(s) 406, 666
BstSLI GKGCMC 1 cut(s) 688
BstX2I RGATCY 1 cut(s) 740
BstYI RGATCY 1 cut(s) 740
BsuRI GGCC 2 cut(s) 341, 402
BtgZI GCGATG 1 cut(s) 405
BtsIMutI CAGTG 1 cut(s) 633
BveI ACCTGC 1 cut(s) 893
CfoI GCGC 1 cut(s) 709
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 400, 413, 424
CseI GACGC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 282
CviAII CATG 1 cut(s) 694
CviQI GTAC 1 cut(s) 282
DdeI CTNAG 3 cut(s) 242, 840, 857
DpnI GATC 2 cut(s) 26, 742
DpnII GATC 2 cut(s) 24, 740
EaeI YGGCCR 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 734
Eco130I CCWWGG 1 cut(s) 783
Eco24I GRGCYC 1 cut(s) 736
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 2 cut(s) 413, 424
Eco53kI GAGCTC 1 cut(s) 734
Eco72I CACGTG 1 cut(s) 689
EcoICRI GAGCTC 1 cut(s) 734
EcoO109I RGGNCCY 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 783
EcoT38I GRGCYC 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 783
FaeI CATG 1 cut(s) 697
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 203, 338
FatI CATG 1 cut(s) 693
FriOI GRGCYC 1 cut(s) 736
FspBI CTAG 5 cut(s) 285, 377, 579, 720, 737
GlaI GCGC 1 cut(s) 708
HaeIII GGCC 2 cut(s) 341, 402
HapII CCGG 1 cut(s) 342
HgaI GACGC 1 cut(s) 281
HhaI GCGC 1 cut(s) 709
Hin1II CATG 1 cut(s) 697
Hin6I GCGC 1 cut(s) 707
HinP1I GCGC 1 cut(s) 707
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 2 cut(s) 305, 561
HpaII CCGG 1 cut(s) 342
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 2 cut(s) 663, 686
Hpy188I TCNGA 4 cut(s) 94, 203, 217, 731
Hpy188III TCNNGA 5 cut(s) 356, 647, 720, 746, 842
Hpy8I GTNNAC 2 cut(s) 663, 686
Hpy99I CGWCG 1 cut(s) 773
HpyAV CCTTC 4 cut(s) 51, 383, 674, 791
HpyCH4III ACNGT 5 cut(s) 223, 335, 637, 667, 726
HpyCH4IV ACGT 3 cut(s) 453, 688, 771
HpyCH4V TGCA 9 cut(s) 14, 67, 139, 548, 653, 686, 704, 853, 888
HpyF10VI GCNNNNNNNGC 8 cut(s) 67, 408, 545, 704, 713, 788, 821, 830
HpyF3I CTNAG 3 cut(s) 242, 840, 857
HpySE526I ACGT 3 cut(s) 453, 688, 771
Hsp92II CATG 1 cut(s) 697
HspAI GCGC 1 cut(s) 707
Kzo9I GATC 2 cut(s) 24, 740
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 9 cut(s) 297, 341, 355, 455, 579, 695, 827, 885, 898
LweI GCATC 4 cut(s) 640, 725, 769, 897
MaeI CTAG 5 cut(s) 285, 377, 579, 720, 737
MaeII ACGT 3 cut(s) 453, 688, 771
MaeIII GTNAC 4 cut(s) 106, 148, 169, 689
MalI GATC 2 cut(s) 26, 742
MboI GATC 2 cut(s) 24, 740
MboII GAAGA 4 cut(s) 147, 250, 300, 689
MflI RGATCY 1 cut(s) 740
MhlI GDGCHC 2 cut(s) 688, 736
MluCI AATT 5 cut(s) 117, 134, 759, 804, 876
MmeI TCCRAC 1 cut(s) 54
MseI TTAA 5 cut(s) 236, 386, 440, 672, 879
MslI CAYNNNNRTG 2 cut(s) 648, 658
MspA1I CMGCKG 1 cut(s) 70
MspI CCGG 1 cut(s) 342
MwoI GCNNNNNNNGC 8 cut(s) 67, 408, 545, 704, 713, 788, 821, 830
NdeII GATC 2 cut(s) 24, 740
NlaIII CATG 1 cut(s) 697
NlaIV GGNNCC 1 cut(s) 426
NmuCI GTSAC 4 cut(s) 106, 148, 169, 689
OliI CACNNNNGTG 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PfeI GAWTC 2 cut(s) 305, 561
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
Ppu21I YACGTR 2 cut(s) 454, 689
Psp124BI GAGCTC 1 cut(s) 736
PspCI CACGTG 1 cut(s) 689
PspN4I GGNNCC 1 cut(s) 426
PspPI GGNCC 3 cut(s) 400, 413, 424
PsuI RGATCY 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 70
RsaI GTAC 1 cut(s) 283
RsaNI GTAC 1 cut(s) 282
RseI CAYNNNNRTG 2 cut(s) 648, 658
SacI GAGCTC 1 cut(s) 736
SaqAI TTAA 5 cut(s) 236, 386, 440, 672, 879
Sau3AI GATC 2 cut(s) 24, 740
Sau96I GGNCC 3 cut(s) 400, 413, 424
ScaI AGTACT 1 cut(s) 283
SduI GDGCHC 2 cut(s) 688, 736
SfaNI GCATC 4 cut(s) 640, 725, 769, 897
SfcI CTRYAG 2 cut(s) 406, 666
SinI GGWCC 2 cut(s) 413, 424
SmiMI CAYNNNNRTG 2 cut(s) 648, 658
SmlI CTYRAG 1 cut(s) 744
SmoI CTYRAG 1 cut(s) 744
SpeI ACTAGT 1 cut(s) 578
Sse9I AATT 5 cut(s) 117, 134, 759, 804, 876
SsiI CCGC 3 cut(s) 411, 513, 522
SspMI CTAG 5 cut(s) 285, 377, 579, 720, 737
SstI GAGCTC 1 cut(s) 736
StyI CCWWGG 1 cut(s) 783
TaaI ACNGT 5 cut(s) 223, 335, 637, 667, 726
TaiI ACGT 3 cut(s) 456, 691, 774
TaqI TCGA 1 cut(s) 768
TaqII GACCGA 1 cut(s) 639
TasI AATT 5 cut(s) 117, 134, 759, 804, 876
TatI WGTACW 1 cut(s) 281
TfiI GAWTC 2 cut(s) 305, 561
Tru1I TTAA 5 cut(s) 236, 386, 440, 672, 879
Tru9I TTAA 5 cut(s) 236, 386, 440, 672, 879
TscAI CASTG 1 cut(s) 640
TseFI GTSAC 4 cut(s) 106, 148, 169, 689
Tsp45I GTSAC 4 cut(s) 106, 148, 169, 689
TspDTI ATGAA 4 cut(s) 279, 632, 810, 834
TspGWI ACGGA 2 cut(s) 188, 437
TspRI CASTG 1 cut(s) 640
VneI GTGCAC 1 cut(s) 684
VpaK11BI GGWCC 2 cut(s) 413, 424
XapI RAATTY 1 cut(s) 759
XbaI TCTAGA 1 cut(s) 719
XspI CTAG 5 cut(s) 285, 377, 579, 720, 737
ZrmI AGTACT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.