pycom12g15500

Ethylene-responsive nuclear protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Reverse (-)
17903513 .. 17904802
1290 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGTCCCTCATTTGCCGCCCAATTATCTGCCTTCTTCCTATGTTGCACACTTGGCCACGCGTCGGTCATTGTCCAACCAGTTTACATCTTCTCGACTTTTCCTTCAGTTTCCTTCTCTCTTACAAATTTGTTCTTCTGTCTACACTCAACTCGCTCTCTCTCGCTCTCCATCTTCATAGAAGCCCTGATTCCTCAACGCTTTTTCAGAACGTGCCAAGTTCGGTTTCAACCGTTCTATCCCGATCTCACAATGTTCGGCGCAAAACCTTGAGAATATTGGCCTCTGCTCCGTCTCCGTCCCCAGAGTTGGGATCGGTCTCGAAGAGCTTTCTGCATCCATCCTTCCGAAGGAACATGCCAAATTGTTTTGAGTGCTTGCAAGATGAAGCTAAGTCTGGGTTGGAATGGCTTCATCAATGTGCAGAAAATGAGCTACAAGTTGACCTCAATGATAAGGACATCAAAAGATTTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

159

Amino Acids

17.94

Weight (kDa)

8.87

Isoelectric Point (pI)

63.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 140
AccII CGCG 1 cut(s) 60
AciI CCGC 1 cut(s) 16
AclWI GGATC 1 cut(s) 319
AcoI YGGCCR 1 cut(s) 53
AcsI RAATTY 1 cut(s) 125
AcuI CTGAAG 1 cut(s) 88
AfiI CCNNNNNNNGG 4 cut(s) 62, 307, 308, 348
AflIII ACRYGT 1 cut(s) 58
AgsI TTSAA 1 cut(s) 228
AjuI GAANNNNNNNTTGG 2 cut(s) 208, 240
AluBI AGCT 3 cut(s) 327, 389, 433
AluI AGCT 3 cut(s) 327, 389, 433
Alw26I GTCTC 2 cut(s) 297, 322
AlwI GGATC 1 cut(s) 319
AoxI GGCC 2 cut(s) 53, 279
ApoI RAATTY 1 cut(s) 125
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 55
BccI CCATC 2 cut(s) 177, 346
BcoDI GTCTC 2 cut(s) 297, 322
BisI GCNGC 1 cut(s) 16
BlsI GCNGC 1 cut(s) 17
BmsI GCATC 1 cut(s) 343
BpuEI CTTGAG 1 cut(s) 289
BsaI GGTCTC 1 cut(s) 322
Bsc4I CCNNNNNNNGG 4 cut(s) 62, 307, 308, 348
Bse1I ACTGG 1 cut(s) 78
BseGI GGATG 2 cut(s) 334, 338
BseLI CCNNNNNNNGG 4 cut(s) 62, 307, 308, 348
BseNI ACTGG 1 cut(s) 78
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 1 cut(s) 60
BshFI GGCC 2 cut(s) 55, 281
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 4 cut(s) 62, 307, 308, 348
BsmAI GTCTC 2 cut(s) 297, 322
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 283
BsnI GGCC 2 cut(s) 55, 281
Bso31I GGTCTC 1 cut(s) 322
Bsp143I GATC 2 cut(s) 242, 311
BspACI CCGC 1 cut(s) 16
BspANI GGCC 2 cut(s) 55, 281
BspFNI CGCG 1 cut(s) 60
BspPI GGATC 1 cut(s) 319
BspQI GCTCTTC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 322
BsrI ACTGG 1 cut(s) 78
BssMI GATC 2 cut(s) 242, 311
Bst4CI ACNGT 1 cut(s) 232
Bst6I CTCTTC 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 1 cut(s) 390
BstENI CCTNNNNNAGG 1 cut(s) 346
BstF5I GGATG 2 cut(s) 334, 338
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 1 cut(s) 261
BstKTI GATC 2 cut(s) 245, 314
BstMAI GTCTC 2 cut(s) 297, 322
BstMBI GATC 2 cut(s) 242, 311
BstMWI GCNNNNNNNGC 1 cut(s) 52
BstNSI RCATGY 1 cut(s) 358
BstUI CGCG 1 cut(s) 60
BsuRI GGCC 2 cut(s) 55, 281
BtsCI GGATG 2 cut(s) 334, 338
Cac8I GCNNGC 1 cut(s) 377
CfoI GCGC 1 cut(s) 261
CseI GACGC 1 cut(s) 49
CviAII CATG 1 cut(s) 355
CviJI RGCY 7 cut(s) 55, 183, 281, 327, 389, 409, 433
CviKI_1 RGCY 7 cut(s) 55, 183, 281, 327, 389, 409, 433
DdeI CTNAG 1 cut(s) 390
DpnI GATC 2 cut(s) 244, 313
DpnII GATC 2 cut(s) 242, 311
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 88
EcoNI CCTNNNNNAGG 1 cut(s) 346
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 1 cut(s) 358
FaiI YATR 3 cut(s) 41, 177, 356
FaqI GGGAC 1 cut(s) 283
FatI CATG 1 cut(s) 354
FblI GTMKAC 1 cut(s) 140
Fnu4HI GCNGC 1 cut(s) 16
FokI GGATG 2 cut(s) 321, 325
Fsp4HI GCNGC 1 cut(s) 16
GlaI GCGC 1 cut(s) 260
GluI GCNGC 1 cut(s) 16
HaeIII GGCC 2 cut(s) 55, 281
HgaI GACGC 1 cut(s) 49
HhaI GCGC 1 cut(s) 261
Hin1II CATG 1 cut(s) 358
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HinfI GANTC 1 cut(s) 188
Hpy166II GTNNAC 3 cut(s) 83, 141, 442
Hpy188I TCNGA 2 cut(s) 207, 347
Hpy188III TCNNGA 3 cut(s) 92, 240, 319
Hpy8I GTNNAC 3 cut(s) 83, 141, 442
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 5 cut(s) 41, 112, 122, 342, 352
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 4 cut(s) 46, 334, 379, 422
HpyF10VI GCNNNNNNNGC 1 cut(s) 52
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 1 cut(s) 358
HspAI GCGC 1 cut(s) 259
Kzo9I GATC 2 cut(s) 242, 311
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 292
LpnPI CCDG 4 cut(s) 91, 198, 315, 381
LweI GCATC 1 cut(s) 343
MaeII ACGT 1 cut(s) 210
MalI GATC 2 cut(s) 244, 313
MboI GATC 2 cut(s) 242, 311
MboII GAAGA 5 cut(s) 26, 80, 125, 164, 334
MlsI TGGCCA 1 cut(s) 55
MluCI AATT 3 cut(s) 21, 125, 361
MluI ACGCGT 1 cut(s) 58
MluNI TGGCCA 1 cut(s) 55
MmeI TCCRAC 2 cut(s) 98, 381
MnlI CCTC 4 cut(s) 17, 202, 292, 455
Mox20I TGGCCA 1 cut(s) 55
MroXI GAANNNNTTC 1 cut(s) 408
MscI TGGCCA 1 cut(s) 55
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 55
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 1 cut(s) 52
NdeII GATC 2 cut(s) 242, 311
NlaIII CATG 1 cut(s) 358
NspI RCATGY 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 1 cut(s) 17
RseI CAYNNNNRTG 1 cut(s) 417
SapI GCTCTTC 1 cut(s) 317
SatI GCNGC 1 cut(s) 16
Sau3AI GATC 2 cut(s) 242, 311
SetI ASST 6 cut(s) 213, 269, 329, 391, 435, 447
SfaNI GCATC 1 cut(s) 343
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 1 cut(s) 268
SmoI CTYRAG 1 cut(s) 268
Sse9I AATT 3 cut(s) 21, 125, 361
SsiI CCGC 1 cut(s) 16
SspI AATATT 1 cut(s) 276
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 93, 320
TaqII GACCGA 2 cut(s) 53, 304
TasI AATT 3 cut(s) 21, 125, 361
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 164, 399, 401, 461
TspGWI ACGGA 2 cut(s) 279, 285
XagI CCTNNNNNAGG 1 cut(s) 346
XapI RAATTY 1 cut(s) 125
XceI RCATGY 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.