pycom12g15870

Domain of unknown function (DUF3453)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
18316869 .. 18318541
1673 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 483 bp
ATGTGTCATCCCTCTCAATTGCTGAGGCCCAGCGGTGTTTGTCATTGTATTTTGCTCTTTGTACAAAGAAGCATTCCCTTTTTCGCCAAATATTTGTTGTTTACGGAAGCGCATCAAAGGCCGTCAAGCAGTTATATGGTGCAGTGCAGGCGGTTCAACGCCAAATTCCAATACTTGTACGTACTATGGGATCGATCACCAAATCTTCTTGAAATTATTTCTGATCCTCCAACTGGAAGCGAGAACCTCCTGATGCAGGTTTTACATACCCTGACGGATGGAACAGTTCCCTCTCAAGAACTGATTTTTACCGTCAGAAAGCTATATGATTCAAAACTGAAGGTACATTTCACTAGCTCGCATACTACAGGTATCTATGTTTCCTTGTTAACTCTTGGCAATTTAAGAATTCTAAGTGTAAGACTGTTCTGTTTCGTATGGGTCATTAGTTATTACAGACAATATGAGAAGATAATGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 ko04530 map03015 map04530
Pfam Domains
Protein Families
DUF3453 Symplekin_C

Protein Analysis

161

Amino Acids

18.7

Weight (kDa)

9.32

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 247
AciI CCGC 2 cut(s) 33, 151
AclWI GGATC 2 cut(s) 198, 218
AcsI RAATTY 2 cut(s) 164, 408
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 4 cut(s) 63, 179, 183, 345
AfiI CCNNNNNNNGG 2 cut(s) 233, 256
AgsI TTSAA 3 cut(s) 157, 212, 333
AluBI AGCT 2 cut(s) 322, 357
AluI AGCT 2 cut(s) 322, 357
AlwI GGATC 2 cut(s) 198, 218
AoxI GGCC 2 cut(s) 26, 119
ApoI RAATTY 2 cut(s) 164, 408
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 23
BccI CCATC 1 cut(s) 272
BceAI ACGGC 1 cut(s) 106
BfaI CTAG 1 cut(s) 354
BfmI CTRYAG 1 cut(s) 366
BfuAI ACCTGC 1 cut(s) 247
BmgT120I GGNCC 1 cut(s) 27
BmsI GCATC 2 cut(s) 121, 243
Bpu10I CCTNAGC 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 279
Bsa29I ATCGAT 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 256
Bse1I ACTGG 1 cut(s) 238
BseCI ATCGAT 1 cut(s) 193
BseGI GGATG 2 cut(s) 7, 283
BseLI CCNNNNNNNGG 2 cut(s) 233, 256
BseMII CTCAG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 238
BseYI CCCAGC 1 cut(s) 29
BsgI GTGCAG 2 cut(s) 161, 166
BshFI GGCC 2 cut(s) 28, 121
BshVI ATCGAT 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 233, 256
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 2 cut(s) 28, 121
Bsp1407I TGTACA 1 cut(s) 61
Bsp143I GATC 3 cut(s) 190, 194, 223
BspACI CCGC 2 cut(s) 33, 151
BspANI GGCC 2 cut(s) 28, 121
BspCNI CTCAG 1 cut(s) 15
BspDI ATCGAT 1 cut(s) 193
BspMI ACCTGC 1 cut(s) 247
BspPI GGATC 2 cut(s) 198, 218
BsrGI TGTACA 1 cut(s) 61
BsrI ACTGG 1 cut(s) 238
BssMI GATC 3 cut(s) 190, 194, 223
Bst4CI ACNGT 3 cut(s) 286, 313, 426
BstAUI TGTACA 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 149, 359
BstDEI CTNAG 2 cut(s) 23, 413
BstENI CCTNNNNNAGG 1 cut(s) 254
BstF5I GGATG 2 cut(s) 7, 283
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 3 cut(s) 193, 197, 226
BstMBI GATC 3 cut(s) 190, 194, 223
BstMWI GCNNNNNNNGC 2 cut(s) 118, 148
BstSFI CTRYAG 1 cut(s) 366
BstSNI TACGTA 1 cut(s) 181
Bsu15I ATCGAT 1 cut(s) 193
BsuRI GGCC 2 cut(s) 28, 121
BsuTUI ATCGAT 1 cut(s) 193
BtsCI GGATG 2 cut(s) 7, 283
BtsI GCAGTG 1 cut(s) 149
BtsIMutI CAGTG 1 cut(s) 149
BveI ACCTGC 1 cut(s) 247
Cac8I GCNNGC 2 cut(s) 149, 359
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 1 cut(s) 27
ClaI ATCGAT 1 cut(s) 193
Csp6I GTAC 4 cut(s) 62, 178, 182, 344
CviJI RGCY 4 cut(s) 28, 121, 322, 357
CviKI_1 RGCY 4 cut(s) 28, 121, 322, 357
CviQI GTAC 4 cut(s) 62, 178, 182, 344
DdeI CTNAG 2 cut(s) 23, 413
DpnI GATC 3 cut(s) 192, 196, 225
DpnII GATC 3 cut(s) 190, 194, 223
Eco105I TACGTA 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 359
EcoNI CCTNNNNNAGG 1 cut(s) 254
EcoRI GAATTC 1 cut(s) 408
FokI GGATG 1 cut(s) 290
FspBI CTAG 1 cut(s) 354
GlaI GCGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 33
HaeIII GGCC 2 cut(s) 28, 121
HhaI GCGC 1 cut(s) 112
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 1 cut(s) 390
HindII GTYRAC 1 cut(s) 390
HinfI GANTC 1 cut(s) 329
HpaI GTTAAC 1 cut(s) 390
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 2 cut(s) 102, 390
Hpy188I TCNGA 2 cut(s) 223, 317
Hpy188III TCNNGA 3 cut(s) 209, 250, 296
Hpy8I GTNNAC 2 cut(s) 102, 390
HpyAV CCTTC 1 cut(s) 334
HpyCH4III ACNGT 3 cut(s) 286, 313, 426
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 142, 147, 256
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 148
HpyF3I CTNAG 2 cut(s) 23, 413
HpySE526I ACGT 1 cut(s) 180
HspAI GCGC 1 cut(s) 110
KspAI GTTAAC 1 cut(s) 390
Kzo9I GATC 3 cut(s) 190, 194, 223
LpnPI CCDG 7 cut(s) 43, 133, 219, 242, 263, 284, 354
LweI GCATC 2 cut(s) 121, 243
MaeI CTAG 1 cut(s) 354
MaeII ACGT 1 cut(s) 180
MalI GATC 3 cut(s) 192, 196, 225
MboI GATC 3 cut(s) 190, 194, 223
MboII GAAGA 2 cut(s) 197, 481
MfeI CAATTG 1 cut(s) 17
MluCI AATT 5 cut(s) 17, 164, 213, 400, 408
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 5 cut(s) 18, 22, 237, 257, 301
MseI TTAA 2 cut(s) 389, 404
MspA1I CMGCKG 1 cut(s) 33
MunI CAATTG 1 cut(s) 17
Mva1269I GAATGC 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 118, 148
NdeII GATC 3 cut(s) 190, 194, 223
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 329
Ppu21I YACGTR 1 cut(s) 181
PspFI CCCAGC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 27
RsaI GTAC 4 cut(s) 63, 179, 183, 345
RsaNI GTAC 4 cut(s) 62, 178, 182, 344
SaqAI TTAA 2 cut(s) 389, 404
Sau3AI GATC 3 cut(s) 190, 194, 223
Sau96I GGNCC 1 cut(s) 27
SetI ASST 7 cut(s) 183, 249, 261, 324, 345, 359, 373
SfaNI GCATC 2 cut(s) 121, 243
SfcI CTRYAG 1 cut(s) 366
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
SnaBI TACGTA 1 cut(s) 181
Sse9I AATT 5 cut(s) 17, 164, 213, 400, 408
SsiI CCGC 2 cut(s) 33, 151
SspI AATATT 1 cut(s) 92
SspMI CTAG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 286, 313, 426
TaiI ACGT 1 cut(s) 183
TaqI TCGA 1 cut(s) 193
TasI AATT 5 cut(s) 17, 164, 213, 400, 408
TatI WGTACW 1 cut(s) 61
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 2 cut(s) 389, 404
Tru9I TTAA 2 cut(s) 389, 404
TscAI CASTG 1 cut(s) 149
TspGWI ACGGA 2 cut(s) 119, 290
TspRI CASTG 1 cut(s) 149
XagI CCTNNNNNAGG 1 cut(s) 254
XapI RAATTY 2 cut(s) 164, 408
XspI CTAG 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.