pycom12g18780

FACT complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
20878496 .. 20879287
792 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGAATTTTCAGAGCTTTGTGAATCGGGTTAATGAGCTTTGGGGGCAGTCACAGTTTAGGGACCTTGAGTTTGATCAACCTCTGAGAGAGCTTGGTTTCCATGGTGTACCTCATAAATCCTCGGCATTTATTGTCCCTACTTCAGCCTGCTTGGTTGAACTGATAGAAACTCCTTTCCTTGTTGTCAGTCTAAGTGAGATTGAGATTGTGAATCTGGAAAGAGTTGGCCTTGGGCAGAAGAATTTTGACATGACAATTGTATTCAAGGACTTCAAAAGGGATGTTCTCCGGATTGATTCAATCCCTTCAACAGCGCTTGATGGTATCAAGGAATGGCTTGACACGACTGACCTCAAGTATTATGAGAGCAGGCTGAATCTGAACTGGAAACAGATACTGAAGACAATCACTGATGACCCGCAGAGCTTCATTGAAGATGGAGGTTGGGAATTTTTGAATTTGGAAGCGTCTGATTCAGAGTCTGAACAGTCGGAGGAGTCAGACCAGGGTTATGAACCATCGGATATAGAATCTGCTGAATCGGAAGAATCAGACGATGAGGATTCTGATAGTGAGTCGGTTGTGGAGTCTGAGGATGATGACGGAGAAGCAGAGTCAGATGAGGATGGGTTATCGTGGGATGAGCTAGAGAGGGAAGCAAGCAATGCAGATAGGGAGAAAGGGAACGAATATGACAGTGAGGAGGACAGGAAGAGAAGGAAGACGAAAGCTTTTGGGAAGTCTCGAGCCCCACCTCCTAGTAGCATCCCGAAGCGGACTAAGATGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000785 GO:0000790 GO:0000791 GO:0003006 GO:0003674 GO:0003682 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005719 GO:0005730 GO:0006139 GO:0006325 GO:0006333 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009791 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031491 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032968 GO:0032991 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034724 GO:0034728 GO:0035101 GO:0042393 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044877 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FACT-Spt16_Nlob Peptidase_M24 Rtt106 SPT16

Protein Analysis

264

Amino Acids

30.08

Weight (kDa)

4.28

Isoelectric Point (pI)

68.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rttp106-like_middle PF08512 34 - 122 2.7e-24 Histone chaperone Rttp106-like, middle domain
SPT16_C PF21091 162 - 241 3.3e-14 FACT complex subunit SPT16, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 288
AciI CCGC 2 cut(s) 419, 775
AcsI RAATTY 4 cut(s) 4, 241, 449, 457
AcuI CTGAAG 2 cut(s) 126, 419
AfaI GTAC 1 cut(s) 108
AfeI AGCGCT 1 cut(s) 315
AgsI TTSAA 7 cut(s) 158, 265, 274, 300, 309, 434, 457
AjnI CCWGG 1 cut(s) 504
AluBI AGCT 6 cut(s) 15, 37, 91, 426, 646, 731
AluI AGCT 6 cut(s) 15, 37, 91, 426, 646, 731
Alw26I GTCTC 1 cut(s) 747
AlwNI CAGNNNCTG 2 cut(s) 397, 482
Ama87I CYCGRG 1 cut(s) 744
Aor13HI TCCGGA 1 cut(s) 288
Aor51HI AGCGCT 1 cut(s) 315
AoxI GGCC 1 cut(s) 226
ApoI RAATTY 4 cut(s) 4, 241, 449, 457
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
AspLEI GCGC 1 cut(s) 316
AspS9I GGNCC 1 cut(s) 61
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 751
BbsI GAAGAC 2 cut(s) 407, 728
BccI CCATC 4 cut(s) 314, 431, 526, 620
BciT130I CCWGG 1 cut(s) 506
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 747
BfaI CTAG 2 cut(s) 647, 759
BfoI RGCGCY 1 cut(s) 317
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 2 cut(s) 774, 774
BpiI GAAGAC 2 cut(s) 407, 728
BpuEI CTTGAG 2 cut(s) 86, 338
BsaJI CCNNGG 4 cut(s) 100, 120, 229, 505
BsaWI WCCGGW 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 670
BseAI TCCGGA 1 cut(s) 288
BseBI CCWGG 1 cut(s) 506
BseDI CCNNGG 4 cut(s) 100, 120, 229, 505
BseGI GGATG 5 cut(s) 286, 601, 631, 646, 765
BseMI GCAATG 1 cut(s) 670
BseMII CTCAG 2 cut(s) 74, 582
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 2 cut(s) 509, 716
BshFI GGCC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 744
BsiSI CCGG 1 cut(s) 289
BslFI GGGAC 2 cut(s) 74, 119
BsmAI GTCTC 1 cut(s) 747
BsmFI GGGAC 2 cut(s) 74, 119
BsnI GGCC 1 cut(s) 228
BsoBI CYCGRG 1 cut(s) 744
Bsp1286I GDGCHC 1 cut(s) 751
Bsp13I TCCGGA 1 cut(s) 288
Bsp143I GATC 1 cut(s) 73
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 2 cut(s) 419, 775
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 2 cut(s) 75, 583
BspEI TCCGGA 1 cut(s) 288
BspLI GGNNCC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 670
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 4 cut(s) 100, 120, 229, 505
BssMI GATC 1 cut(s) 73
BssT1I CCWWGG 2 cut(s) 100, 229
Bst2UI CCWGG 1 cut(s) 506
Bst4CI ACNGT 3 cut(s) 54, 489, 698
Bst6I CTCTTC 1 cut(s) 707
BstAPI GCANNNNNTGC 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 148, 371, 661
BstDEI CTNAG 4 cut(s) 83, 191, 591, 780
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 5 cut(s) 286, 601, 631, 646, 765
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 1 cut(s) 76
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 43, 665
BstNI CCWGG 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 504
BstV2I GAAGAC 2 cut(s) 407, 728
BsuRI GGCC 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 5 cut(s) 286, 601, 631, 646, 765
BtsIMutI CAGTG 2 cut(s) 408, 703
Cac8I GCNNGC 3 cut(s) 148, 371, 661
CaiI CAGNNNCTG 2 cut(s) 397, 482
CfoI GCGC 1 cut(s) 316
Cfr13I GGNCC 1 cut(s) 61
CseI GACGC 1 cut(s) 456
Csp6I GTAC 1 cut(s) 107
CviAII CATG 2 cut(s) 101, 250
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 4 cut(s) 83, 191, 591, 780
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
Eam1104I CTCTTC 1 cut(s) 707
EarI CTCTTC 1 cut(s) 707
Eco130I CCWWGG 2 cut(s) 100, 229
Eco24I GRGCYC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 61
Eco47III AGCGCT 1 cut(s) 315
Eco57I CTGAAG 2 cut(s) 126, 419
Eco88I CYCGRG 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 2 cut(s) 100, 229
EcoT38I GRGCYC 1 cut(s) 751
ErhI CCWWGG 2 cut(s) 100, 229
FaeI CATG 2 cut(s) 104, 253
FaiI YATR 7 cut(s) 102, 114, 251, 363, 513, 527, 693
FaqI GGGAC 2 cut(s) 74, 119
FatI CATG 2 cut(s) 100, 249
FauI CCCGC 1 cut(s) 426
FbaI TGATCA 1 cut(s) 73
FokI GGATG 5 cut(s) 293, 608, 638, 653, 752
FriOI GRGCYC 1 cut(s) 751
FspBI CTAG 2 cut(s) 647, 759
GlaI GCGC 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 289
HgaI GACGC 1 cut(s) 456
HhaI GCGC 1 cut(s) 316
Hin1II CATG 2 cut(s) 104, 253
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HindIII AAGCTT 1 cut(s) 729
HpaII CCGG 1 cut(s) 289
Hpy166II GTNNAC 1 cut(s) 107
Hpy188III TCNNGA 4 cut(s) 215, 289, 744, 769
Hpy8I GTNNAC 1 cut(s) 107
HpyAV CCTTC 2 cut(s) 315, 711
HpyCH4III ACNGT 3 cut(s) 54, 489, 698
HpyCH4V TGCA 1 cut(s) 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 43, 665
HpyF3I CTNAG 4 cut(s) 83, 191, 591, 780
Hsp92II CATG 2 cut(s) 104, 253
HspAI GCGC 1 cut(s) 314
Kpn2I TCCGGA 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 1 cut(s) 73
LpnPI CCDG 8 cut(s) 160, 200, 302, 355, 370, 491, 518, 694
LweI GCATC 2 cut(s) 774, 774
MaeI CTAG 2 cut(s) 647, 759
MaeIII GTNAC 1 cut(s) 48
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 6 cut(s) 250, 412, 446, 557, 724, 733
MfeI CAATTG 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 751
MluCI AATT 5 cut(s) 4, 241, 255, 449, 457
MlyI GAGTC 5 cut(s) 488, 506, 584, 596, 623
MmeI TCCRAC 1 cut(s) 471
MroI TCCGGA 1 cut(s) 288
MseI TTAA 1 cut(s) 30
MspI CCGG 1 cut(s) 289
MspR9I CCNGG 1 cut(s) 506
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 2 cut(s) 43, 665
NcoI CCATGG 1 cut(s) 100
NdeII GATC 1 cut(s) 73
NlaIII CATG 2 cut(s) 104, 253
NlaIV GGNNCC 1 cut(s) 62
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 1 cut(s) 48
PaeR7I CTCGAG 1 cut(s) 744
PfeI GAWTC 9 cut(s) 22, 211, 296, 376, 473, 530, 539, 548, 563
PleI GAGTC 5 cut(s) 487, 505, 583, 595, 622
PpsI GAGTC 5 cut(s) 487, 505, 583, 595, 622
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
PstNI CAGNNNCTG 2 cut(s) 397, 482
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 1 cut(s) 30
Sau3AI GATC 1 cut(s) 73
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 5 cut(s) 488, 506, 584, 596, 623
ScrFI CCNGG 1 cut(s) 506
SduI GDGCHC 1 cut(s) 751
SfaNI GCATC 2 cut(s) 774, 774
Sfr274I CTCGAG 1 cut(s) 744
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 744
SmlI CTYRAG 3 cut(s) 65, 353, 744
SmoI CTYRAG 3 cut(s) 65, 353, 744
Sse9I AATT 5 cut(s) 4, 241, 255, 449, 457
SsiI CCGC 2 cut(s) 419, 775
SspMI CTAG 2 cut(s) 647, 759
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 2 cut(s) 100, 229
TaaI ACNGT 3 cut(s) 54, 489, 698
TaqI TCGA 1 cut(s) 745
TasI AATT 5 cut(s) 4, 241, 255, 449, 457
TfiI GAWTC 9 cut(s) 22, 211, 296, 376, 473, 530, 539, 548, 563
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 2 cut(s) 415, 703
TseFI GTSAC 1 cut(s) 48
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 3 cut(s) 17, 418, 528
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 2 cut(s) 415, 703
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 4 cut(s) 4, 241, 449, 457
XhoI CTCGAG 1 cut(s) 744
XspI CTAG 2 cut(s) 647, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.