pycom12g22540

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
23395446 .. 23395847
402 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 306 bp
ATGAAGAAAGATGCCTTACTTCCGGCTGATTATTTAATGCCATGGATGCAGGATGATAGCTTGAAATACACTGAAGCTGTTCTGGATGACTCCCTCAAAGAAAATCTCGAGCACCTTGAGAAGATTGGGAATCAACTCCTCGAAAAACCTTTTTCAGCACTGAACCTGGAGACCGGCTTGTTGGAACCAATTAACAACACATTCAAATACAAAGACGCACTTGCACATAACTCGTTGCATTTGTACAGGTTCGCCAAGAAACTGTCAGATGAAAAAAGGGGATGTCAAACCAGTTCCTCCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

102

Amino Acids

11.63

Weight (kDa)

5.88

Isoelectric Point (pI)

27.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 1 cut(s) 245
AgsI TTSAA 2 cut(s) 64, 205
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 60, 77
AluI AGCT 2 cut(s) 60, 77
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 107
Asp700I GAANNNNTTC 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 107
Bbv12I GWGCWC 1 cut(s) 114
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BciT130I CCWGG 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 164
Bme1390I CCNGG 1 cut(s) 167
BmeT110I CYCGRG 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 36
BpmI CTGGAG 1 cut(s) 188
BpuEI CTTGAG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 41
Bse118I RCCGGY 1 cut(s) 173
Bse1I ACTGG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 41
BseGI GGATG 4 cut(s) 51, 58, 91, 287
BseNI ACTGG 1 cut(s) 291
BseRI GAGGAG 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 23, 174
BsmAI GTCTC 1 cut(s) 164
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 114
Bsp1407I TGTACA 1 cut(s) 243
Bsp19I CCATGG 1 cut(s) 41
BspLI GGNNCC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 291
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 264
BstAUI TGTACA 1 cut(s) 243
BstDSI CCRYGG 1 cut(s) 41
BstF5I GGATG 4 cut(s) 51, 58, 91, 287
BstMAI GTCTC 1 cut(s) 164
BstMWI GCNNNNNNNGC 1 cut(s) 46
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 41
BtsCI GGATG 4 cut(s) 51, 58, 91, 287
BtsIMutI CAGTG 2 cut(s) 69, 158
Cfr10I RCCGGY 1 cut(s) 173
CseI GACGC 1 cut(s) 224
Csp6I GTAC 1 cut(s) 244
CviAII CATG 1 cut(s) 42
CviJI RGCY 4 cut(s) 26, 60, 77, 177
CviKI_1 RGCY 4 cut(s) 26, 60, 77, 177
CviQI GTAC 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 41
Eco31I GGTCTC 1 cut(s) 164
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 41
ErhI CCWWGG 1 cut(s) 41
FaeI CATG 1 cut(s) 45
FaiI YATR 2 cut(s) 43, 228
FalI AAGNNNNNCTT 2 cut(s) 204, 236
FatI CATG 1 cut(s) 41
FokI GGATG 4 cut(s) 58, 65, 98, 294
GsuI CTGGAG 1 cut(s) 188
HapII CCGG 2 cut(s) 23, 174
HgaI GACGC 1 cut(s) 224
Hin1II CATG 1 cut(s) 45
HinfI GANTC 2 cut(s) 89, 130
HpaII CCGG 2 cut(s) 23, 174
Hpy188I TCNGA 1 cut(s) 268
Hpy188III TCNNGA 2 cut(s) 83, 107
HpyCH4III ACNGT 1 cut(s) 264
HpyCH4V TGCA 3 cut(s) 49, 224, 238
HpyF10VI GCNNNNNNNGC 1 cut(s) 46
Hsp92II CATG 1 cut(s) 45
LpnPI CCDG 7 cut(s) 35, 36, 68, 152, 179, 187, 232
LweI GCATC 1 cut(s) 36
MboII GAAGA 2 cut(s) 16, 133
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 1 cut(s) 189
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 2 cut(s) 104, 149
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 2 cut(s) 35, 192
MspI CCGG 2 cut(s) 23, 174
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 46
NcoI CCATGG 1 cut(s) 41
NlaIII CATG 1 cut(s) 45
NlaIV GGNNCC 1 cut(s) 186
PaeR7I CTCGAG 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 130
PleI GAGTC 1 cut(s) 83
PpsI GAGTC 1 cut(s) 83
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 1 cut(s) 186
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
SaqAI TTAA 2 cut(s) 35, 192
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 167
SduI GDGCHC 1 cut(s) 114
SetI ASST 6 cut(s) 62, 79, 117, 151, 168, 251
SfaNI GCATC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 107, 116
SmoI CTYRAG 2 cut(s) 107, 116
Sse9I AATT 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 264
TaqI TCGA 2 cut(s) 108, 141
TasI AATT 1 cut(s) 189
TatI WGTACW 1 cut(s) 243
TfiI GAWTC 1 cut(s) 130
Tru1I TTAA 2 cut(s) 35, 192
Tru9I TTAA 2 cut(s) 35, 192
TscAI CASTG 2 cut(s) 76, 165
TspDTI ATGAA 2 cut(s) 17, 285
TspRI CASTG 2 cut(s) 76, 165
XhoI CTCGAG 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.