pycom12g24050

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
24449866 .. 24450162
297 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 297 bp
ATGGCGCTCTCTCGCTCCTCCTCTCAGCCGTCCCACCGGCGGTTGATCTTCGTAATCTCCGCCGTGCTCTGTATTACAGTAGCAGCAGCTTTCCAATCCGATGAGCTCCTGGTGGACGACGAGGAGTTCGGCCTAGAAGGAGGGCTGCACACCAAGTCTCCTGATCTCACATTCACACGATCCACGCCTCCGCCACCATCGACAACTGCATCGACGACCCGGAAGAGGTTTTCGGATCCGGATTCGGACTCAAAGATCGATCCAGTTCCAGCTCCATCACGCCTTTGGGGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

10.61

Weight (kDa)

5.54

Isoelectric Point (pI)

76.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 238
AciI CCGC 3 cut(s) 40, 60, 191
AclWI GGATC 4 cut(s) 174, 230, 243, 254
AfiI CCNNNNNNNGG 2 cut(s) 39, 225
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 3 cut(s) 89, 106, 272
AluI AGCT 3 cut(s) 89, 106, 272
Alw21I GWGCWC 2 cut(s) 69, 108
Alw26I GTCTC 1 cut(s) 162
AlwI GGATC 4 cut(s) 174, 230, 243, 254
Aor13HI TCCGGA 1 cut(s) 238
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 83, 86, 145
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 220
BamHI GGATCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 108
Bbv12I GWGCWC 2 cut(s) 69, 108
BbvI GCAGC 3 cut(s) 95, 98, 132
BccI CCATC 2 cut(s) 205, 283
BceAI ACGGC 2 cut(s) 13, 47
BciT130I CCWGG 1 cut(s) 110
BcnI CCSGG 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 84, 87, 146
BlsI GCNGC 3 cut(s) 85, 88, 147
Bme1390I CCNGG 2 cut(s) 110, 220
BmiI GGNNCC 1 cut(s) 237
BmrFI CCNGG 2 cut(s) 110, 220
BmsI GCATC 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 220
Bsa29I ATCGAT 1 cut(s) 258
BsaWI WCCGGW 1 cut(s) 238
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 225
Bse118I RCCGGY 1 cut(s) 36
Bse1I ACTGG 1 cut(s) 263
BseAI TCCGGA 1 cut(s) 238
BseBI CCWGG 1 cut(s) 110
BseCI ATCGAT 1 cut(s) 258
BseLI CCNNNNNNNGG 2 cut(s) 39, 225
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 3 cut(s) 7, 10, 137
BseXI GCAGC 3 cut(s) 95, 98, 132
BsgI GTGCAG 1 cut(s) 131
BshFI GGCC 1 cut(s) 132
BshVI ATCGAT 1 cut(s) 258
BsiHKAI GWGCWC 2 cut(s) 69, 108
BsiSI CCGG 3 cut(s) 37, 220, 239
BslFI GGGAC 1 cut(s) 16
BslI CCNNNNNNNGG 2 cut(s) 39, 225
BsmAI GTCTC 1 cut(s) 162
BsmFI GGGAC 1 cut(s) 16
BsnI GGCC 1 cut(s) 132
Bsp1286I GDGCHC 2 cut(s) 69, 108
Bsp13I TCCGGA 1 cut(s) 238
Bsp143I GATC 6 cut(s) 45, 163, 179, 235, 255, 259
BspACI CCGC 3 cut(s) 40, 60, 191
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 1 cut(s) 37
BspDI ATCGAT 1 cut(s) 258
BspEI TCCGGA 1 cut(s) 238
BspLI GGNNCC 1 cut(s) 237
BspPI GGATC 4 cut(s) 174, 230, 243, 254
BsrFI RCCGGY 1 cut(s) 36
BsrI ACTGG 1 cut(s) 263
BssAI RCCGGY 1 cut(s) 36
BssMI GATC 6 cut(s) 45, 163, 179, 235, 255, 259
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 48, 166, 182, 238, 258, 262
BstMAI GTCTC 1 cut(s) 162
BstMBI GATC 6 cut(s) 45, 163, 179, 235, 255, 259
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 2 cut(s) 108, 218
BstV1I GCAGC 3 cut(s) 95, 98, 132
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
Bsu15I ATCGAT 1 cut(s) 258
BsuRI GGCC 1 cut(s) 132
BsuTUI ATCGAT 1 cut(s) 258
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 36
ClaI ATCGAT 1 cut(s) 258
CviJI RGCY 6 cut(s) 28, 89, 106, 132, 145, 272
CviKI_1 RGCY 6 cut(s) 28, 89, 106, 132, 145, 272
DdeI CTNAG 1 cut(s) 24
DpnI GATC 6 cut(s) 47, 165, 181, 237, 257, 261
DpnII GATC 6 cut(s) 45, 163, 179, 235, 255, 259
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
EciI GGCGGA 2 cut(s) 49, 180
Ecl136II GAGCTC 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 108
FaqI GGGAC 1 cut(s) 16
Fnu4HI GCNGC 3 cut(s) 84, 87, 146
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 3 cut(s) 84, 87, 146
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 84, 87, 146
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 132
HapII CCGG 3 cut(s) 37, 220, 239
HhaI GCGC 1 cut(s) 7
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 3 cut(s) 242, 248, 291
HpaII CCGG 3 cut(s) 37, 220, 239
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 4 cut(s) 100, 235, 247, 296
Hpy188III TCNNGA 2 cut(s) 161, 239
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 2 cut(s) 122, 217
HpyAV CCTTC 1 cut(s) 131
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4V TGCA 2 cut(s) 148, 209
HpyF3I CTNAG 1 cut(s) 24
HspAI GCGC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 238
Kzo9I GATC 6 cut(s) 45, 163, 179, 235, 255, 259
LmnI GCTCC 3 cut(s) 20, 111, 277
LpnPI CCDG 8 cut(s) 50, 95, 122, 174, 233, 252, 276, 282
Lsp1109I GCAGC 3 cut(s) 95, 98, 132
LweI GCATC 1 cut(s) 218
MaeI CTAG 1 cut(s) 134
MalI GATC 6 cut(s) 47, 165, 181, 237, 257, 261
MboI GATC 6 cut(s) 45, 163, 179, 235, 255, 259
MboII GAAGA 2 cut(s) 40, 235
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 2 cut(s) 69, 108
MlyI GAGTC 2 cut(s) 242, 285
MnlI CCTC 6 cut(s) 28, 31, 115, 134, 198, 219
MroI TCCGGA 1 cut(s) 238
MspI CCGG 3 cut(s) 37, 220, 239
MspR9I CCNGG 2 cut(s) 110, 220
MvaI CCWGG 1 cut(s) 110
NciI CCSGG 1 cut(s) 220
NdeII GATC 6 cut(s) 45, 163, 179, 235, 255, 259
NlaIV GGNNCC 1 cut(s) 237
PfeI GAWTC 1 cut(s) 242
PkrI GCNGC 3 cut(s) 85, 88, 147
PleI GAGTC 2 cut(s) 242, 285
PpsI GAGTC 2 cut(s) 242, 285
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 237
PsuI RGATCY 1 cut(s) 235
SacI GAGCTC 1 cut(s) 108
SatI GCNGC 3 cut(s) 84, 87, 146
Sau3AI GATC 6 cut(s) 45, 163, 179, 235, 255, 259
SchI GAGTC 2 cut(s) 242, 285
ScrFI CCNGG 2 cut(s) 110, 220
SduI GDGCHC 2 cut(s) 69, 108
SetI ASST 4 cut(s) 91, 108, 230, 274
SfaNI GCATC 1 cut(s) 218
SgrAI CRCCGGYG 1 cut(s) 36
SsiI CCGC 3 cut(s) 40, 60, 191
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 2 cut(s) 108, 218
TaaI ACNGT 1 cut(s) 79
TaqI TCGA 3 cut(s) 200, 212, 258
TfiI GAWTC 1 cut(s) 242
TseI GCWGC 3 cut(s) 83, 86, 145
XcmI CCANNNNNNNNNTGG 1 cut(s) 282
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.