pycom12g24220

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
N/A
Physical Location & Seq
Forward (+)
24567181 .. 24570532
3352 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGGCTGTGAGTCTGAATTCGATGCTGACCTTCCCGTCTCACAGCCTCACTGCCAAGTCCCACAATCTCAGATCTCCCAAAATCGTCATGGCCTCCACTCTTCACTCCTCCACCAAAGAGACTGAGAATCTGAAGAAGTCTTTTGGCCCTCCACGTGAGGTACATGTTCAGGTGACTCATTCAATGCCTCCTCAACAGATTGAGATCTTCAAGTCATTAGAGGATTGGGCTGAGAATAACATCTTGGTGCACCTAAAGCCTGTTGAGAAATGTTGGCAACCACAAGATTTTCTTCCACTTCCTGAGTCAGATGGATTCGAGGAGCAAGTTAAGGAGTTGAGGGAAAGAGCAAGGGAAATTCCTGATGATTACTTTGTTGTGTTGGTTGGTGATATGATCACAGAAGAAGCCCTTCCAACGTACCAGACAATGCTTAATACCCTAGATGGAGTTCGGGATGAGACTGGTGCAAGCCTTACTTCCTGGGCTATTTGGACCAGGGCGTGGACTGCTGAAGAGAATAGGCATGGTGATCTTCTTAACAAGTATCTTTATCTCTCTGGACGAGTAGATATGAGACAAATCGAGAAAACAATTCAGTACCTGATGATCGATCTGGAATGGATCCTAAAACAGAGAACAATCCCTACCTTGGGTTCATTTACACTTCATTCCAAGAGAGGGCAACATTCATCTCCCATGGGAACACAGCCAGGCAAGCCAAAGAGCATGGGGACCTGA

Protein Analysis

247

Amino Acids

28.11

Weight (kDa)

6.15

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 66 - 203 2.2e-67 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 34
AccB7I CCANNNNNTGG 1 cut(s) 504
AclWI GGATC 2 cut(s) 619, 632
AcsI RAATTY 2 cut(s) 16, 357
AcuI CTGAAG 2 cut(s) 152, 534
AcvI CACGTG 1 cut(s) 155
AfaI GTAC 3 cut(s) 162, 422, 602
AfiI CCNNNNNNNGG 4 cut(s) 504, 652, 653, 681
AflIII ACRYGT 1 cut(s) 163
AgsI TTSAA 2 cut(s) 183, 211
AjnI CCWGG 3 cut(s) 482, 497, 712
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
Alw21I GWGCWC 1 cut(s) 252
Alw26I GTCTC 4 cut(s) 42, 113, 455, 571
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 2 cut(s) 619, 632
AlwNI CAGNNNCTG 1 cut(s) 604
AoxI GGCC 2 cut(s) 90, 145
ApaLI GTGCAC 1 cut(s) 248
ApoI RAATTY 2 cut(s) 16, 357
Asp700I GAANNNNTTC 1 cut(s) 411
AspS9I GGNCC 3 cut(s) 146, 495, 735
AsuHPI GGTGA 3 cut(s) 184, 401, 542
AvaII GGWCC 2 cut(s) 495, 735
BaeGI GKGCMC 1 cut(s) 252
BamHI GGATCC 1 cut(s) 624
BbrPI CACGTG 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 252
BccI CCATC 2 cut(s) 305, 440
BciT130I CCWGG 3 cut(s) 484, 499, 714
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 4 cut(s) 42, 113, 455, 571
BfaI CTAG 1 cut(s) 443
BglII AGATCT 2 cut(s) 71, 204
Bme1390I CCNGG 3 cut(s) 484, 499, 714
Bme18I GGWCC 2 cut(s) 495, 735
BmgT120I GGNCC 3 cut(s) 146, 495, 735
BmiI GGNNCC 2 cut(s) 626, 736
BmrFI CCNGG 3 cut(s) 484, 499, 714
BmsI GCATC 1 cut(s) 12
Bsa29I ATCGAT 1 cut(s) 612
BsaAI YACGTR 1 cut(s) 155
BsaBI GATNNNNATC 1 cut(s) 203
BsaJI CCNNGG 4 cut(s) 483, 498, 651, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 504, 652, 653, 681
Bse1I ACTGG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 3 cut(s) 484, 499, 714
BseCI ATCGAT 1 cut(s) 612
BseDI CCNNGG 4 cut(s) 483, 498, 651, 699
BseGI GGATG 1 cut(s) 463
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 4 cut(s) 504, 652, 653, 681
BseMII CTCAG 4 cut(s) 82, 114, 222, 294
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 3 cut(s) 97, 180, 335
BseSI GKGCMC 1 cut(s) 252
BshFI GGCC 2 cut(s) 92, 147
BshVI ATCGAT 1 cut(s) 612
BsiHKAI GWGCWC 1 cut(s) 252
BslFI GGGAC 1 cut(s) 43
BslI CCNNNNNNNGG 4 cut(s) 504, 652, 653, 681
BsmAI GTCTC 4 cut(s) 42, 113, 455, 571
BsmBI CGTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 43
BsnI GGCC 2 cut(s) 92, 147
Bsp1286I GDGCHC 1 cut(s) 252
Bsp143I GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
Bsp19I CCATGG 1 cut(s) 699
BspANI GGCC 2 cut(s) 92, 147
BspCNI CTCAG 4 cut(s) 81, 115, 223, 295
BspDI ATCGAT 1 cut(s) 612
BspLI GGNNCC 2 cut(s) 626, 736
BspPI GGATC 2 cut(s) 619, 632
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 4 cut(s) 483, 498, 651, 699
BssMI GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
BssT1I CCWWGG 2 cut(s) 651, 699
Bst2UI CCWGG 3 cut(s) 484, 499, 714
Bst6I CTCTTC 2 cut(s) 105, 510
BstBAI YACGTR 1 cut(s) 155
BstC8I GCNNGC 2 cut(s) 472, 719
BstDEI CTNAG 4 cut(s) 68, 123, 231, 303
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 1 cut(s) 463
BstKTI GATC 7 cut(s) 74, 207, 399, 535, 612, 616, 627
BstMAI GTCTC 4 cut(s) 42, 113, 455, 571
BstMBI GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
BstMWI GCNNNNNNNGC 3 cut(s) 256, 509, 718
BstNI CCWGG 3 cut(s) 484, 499, 714
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 482, 497, 712
BstSLI GKGCMC 1 cut(s) 252
BstX2I RGATCY 3 cut(s) 71, 204, 624
BstYI RGATCY 3 cut(s) 71, 204, 624
Bsu15I ATCGAT 1 cut(s) 612
BsuRI GGCC 2 cut(s) 92, 147
BsuTUI ATCGAT 1 cut(s) 612
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 1 cut(s) 463
BtsI GCAGTG 1 cut(s) 48
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 2 cut(s) 472, 719
CaiI CAGNNNCTG 1 cut(s) 604
Cfr13I GGNCC 3 cut(s) 146, 495, 735
ClaI ATCGAT 1 cut(s) 612
Csp6I GTAC 3 cut(s) 161, 421, 601
CviAII CATG 5 cut(s) 88, 164, 527, 700, 730
CviQI GTAC 3 cut(s) 161, 421, 601
DdeI CTNAG 4 cut(s) 68, 123, 231, 303
DpnI GATC 7 cut(s) 73, 206, 398, 534, 611, 615, 626
DpnII GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
DrdI GACNNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 105, 510
EarI CTCTTC 2 cut(s) 105, 510
Eco130I CCWWGG 2 cut(s) 651, 699
Eco47I GGWCC 2 cut(s) 495, 735
Eco57I CTGAAG 2 cut(s) 152, 534
Eco72I CACGTG 1 cut(s) 155
EcoO109I RGGNCCY 1 cut(s) 735
EcoRI GAATTC 1 cut(s) 16
EcoRII CCWGG 3 cut(s) 482, 497, 712
EcoT14I CCWWGG 2 cut(s) 651, 699
ErhI CCWWGG 2 cut(s) 651, 699
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 5 cut(s) 91, 167, 530, 703, 733
FaiI YATR 7 cut(s) 89, 165, 395, 528, 575, 701, 731
FalI AAGNNNNNCTT 6 cut(s) 276, 308, 396, 428, 463, 495
FaqI GGGAC 1 cut(s) 43
FatI CATG 5 cut(s) 87, 163, 526, 699, 729
FbaI TGATCA 1 cut(s) 396
FokI GGATG 1 cut(s) 470
FspBI CTAG 1 cut(s) 443
HaeIII GGCC 2 cut(s) 92, 147
Hin1II CATG 5 cut(s) 91, 167, 530, 703, 733
HinfI GANTC 5 cut(s) 10, 127, 175, 305, 315
HphI GGTGA 3 cut(s) 184, 401, 542
Hpy166II GTNNAC 2 cut(s) 250, 507
Hpy188I TCNGA 4 cut(s) 15, 71, 132, 310
Hpy188III TCNNGA 6 cut(s) 302, 362, 455, 561, 586, 617
Hpy8I GTNNAC 2 cut(s) 250, 507
HpyAV CCTTC 2 cut(s) 40, 422
HpyCH4IV ACGT 2 cut(s) 154, 419
HpyCH4V TGCA 2 cut(s) 250, 470
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 509, 718
HpyF3I CTNAG 4 cut(s) 68, 123, 231, 303
HpySE526I ACGT 2 cut(s) 154, 419
Hsp92II CATG 5 cut(s) 91, 167, 530, 703, 733
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
LmnI GCTCC 1 cut(s) 322
LweI GCATC 1 cut(s) 12
MaeI CTAG 1 cut(s) 443
MaeII ACGT 2 cut(s) 154, 419
MaeIII GTNAC 1 cut(s) 172
MalI GATC 7 cut(s) 73, 206, 398, 534, 611, 615, 626
MboI GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
MboII GAAGA 7 cut(s) 92, 145, 199, 284, 416, 527, 527
MflI RGATCY 3 cut(s) 71, 204, 624
MhlI GDGCHC 1 cut(s) 252
MluCI AATT 3 cut(s) 16, 357, 594
MlyI GAGTC 3 cut(s) 19, 169, 314
MmeI TCCRAC 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 411
MseI TTAA 3 cut(s) 330, 435, 540
MslI CAYNNNNRTG 1 cut(s) 245
MspR9I CCNGG 3 cut(s) 484, 499, 714
MvaI CCWGG 3 cut(s) 484, 499, 714
MwoI GCNNNNNNNGC 3 cut(s) 256, 509, 718
NcoI CCATGG 1 cut(s) 699
NdeII GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
NlaIII CATG 5 cut(s) 91, 167, 530, 703, 733
NlaIV GGNNCC 2 cut(s) 626, 736
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 167
PciI ACATGT 1 cut(s) 163
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 127, 315
PflMI CCANNNNNTGG 1 cut(s) 504
PleI GAGTC 3 cut(s) 18, 169, 313
PmaCI CACGTG 1 cut(s) 155
PmlI CACGTG 1 cut(s) 155
PpsI GAGTC 3 cut(s) 18, 169, 313
Ppu21I YACGTR 1 cut(s) 155
PpuMI RGGWCCY 1 cut(s) 735
PscI ACATGT 1 cut(s) 163
Psp5II RGGWCCY 1 cut(s) 735
Psp6I CCWGG 3 cut(s) 482, 497, 712
PspCI CACGTG 1 cut(s) 155
PspGI CCWGG 3 cut(s) 482, 497, 712
PspN4I GGNNCC 2 cut(s) 626, 736
PspPI GGNCC 3 cut(s) 146, 495, 735
PspPPI RGGWCCY 1 cut(s) 735
PsrI GAACNNNNNNTAC 2 cut(s) 631, 663
PstNI CAGNNNCTG 1 cut(s) 604
PsuI RGATCY 3 cut(s) 71, 204, 624
RsaI GTAC 3 cut(s) 162, 422, 602
RsaNI GTAC 3 cut(s) 161, 421, 601
RseI CAYNNNNRTG 1 cut(s) 245
SaqAI TTAA 3 cut(s) 330, 435, 540
Sau3AI GATC 7 cut(s) 71, 204, 396, 532, 609, 613, 624
Sau96I GGNCC 3 cut(s) 146, 495, 735
SchI GAGTC 3 cut(s) 19, 169, 314
ScrFI CCNGG 3 cut(s) 484, 499, 714
SduI GDGCHC 1 cut(s) 252
SetI ASST 9 cut(s) 32, 157, 162, 174, 255, 422, 606, 653, 740
SfaNI GCATC 1 cut(s) 12
SinI GGWCC 2 cut(s) 495, 735
SmiMI CAYNNNNRTG 1 cut(s) 245
Sse9I AATT 3 cut(s) 16, 357, 594
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 482, 497, 712
StyI CCWWGG 2 cut(s) 651, 699
TaiI ACGT 2 cut(s) 157, 422
TaqI TCGA 4 cut(s) 20, 318, 585, 612
TasI AATT 3 cut(s) 16, 357, 594
TfiI GAWTC 2 cut(s) 127, 315
Tru1I TTAA 3 cut(s) 330, 435, 540
Tru9I TTAA 3 cut(s) 330, 435, 540
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 648, 659, 681
TspRI CASTG 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 504
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 495, 735
XapI RAATTY 2 cut(s) 16, 357
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.