pycom133g00180

Aminoacyl-tRNA editing domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000133
N/A
Physical Location & Seq
Reverse (-)
374638 .. 375136
499 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 426 bp
ATGGCTGGCCTCCACCGAAGCCCAGCTAATTGGATGGGGTGGAGTGGGCTTTTGGCGGGAGGGGAAAAATGGGCTGTTGCCCAGCTTATTTGTGGAGGGCTGGAGTTGCTCCAAGGAGAACGTCGTCCTCATAGCGAATCCAAAACCCAAATCCTCGAACGCATATCAAAGCTTGAGCTCTCCATCTCCACTTCTCTTCCGCAATTCACCGCTACCCCCATCCCTACCTCAACTCAAAACGCCGCCGTCAGCGCCAACGAAGCCCGCCTCTCCGCCATTCTCCTAGCCAATGACGTAAAAAACTTCGCCTTCAAGAGGGTTCCCTCCGATTACTACGATTGGTCTCTCGACGCCCGGCGCTGCCTCAATCCACCATATCTGCAAAAGCATTGTCTTGATAGTTGGTACCTAACTCAATCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
tRNA_edit

Protein Analysis

142

Amino Acids

15.69

Weight (kDa)

8.51

Isoelectric Point (pI)

52.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 405
AccB1I GGYRCC 1 cut(s) 405
AciI CCGC 6 cut(s) 56, 200, 210, 243, 265, 273
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 315
AgsI TTSAA 1 cut(s) 313
AluBI AGCT 4 cut(s) 26, 85, 172, 178
AluI AGCT 4 cut(s) 26, 85, 172, 178
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 1 cut(s) 348
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 1 cut(s) 360
ArsI GACNNNNNNTTYG 2 cut(s) 376, 408
Asp718I GGTACC 1 cut(s) 405
AspLEI GCGC 2 cut(s) 254, 360
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 1 cut(s) 199
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 347
BccI CCATC 3 cut(s) 28, 191, 227
BceAI ACGGC 1 cut(s) 230
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 1 cut(s) 284
BfoI RGCGCY 2 cut(s) 255, 361
BisI GCNGC 2 cut(s) 243, 361
BlsI GCNGC 2 cut(s) 244, 362
Bme1390I CCNGG 1 cut(s) 355
BmiI GGNNCC 2 cut(s) 321, 407
BmrFI CCNGG 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 194
BpuMI CCSGG 1 cut(s) 355
BsaHI GRCGYC 1 cut(s) 351
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 2 cut(s) 39, 219
BseLI CCNNNNNNNGG 1 cut(s) 315
BseXI GCAGC 1 cut(s) 347
BseYI CCCAGC 2 cut(s) 22, 81
BshFI GGCC 1 cut(s) 9
BshNI GGYRCC 1 cut(s) 405
BsiHKAI GWGCWC 1 cut(s) 180
BsiSI CCGG 1 cut(s) 355
BslI CCNNNNNNNGG 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 348
BsnI GGCC 1 cut(s) 9
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 1 cut(s) 180
BspACI CCGC 6 cut(s) 56, 200, 210, 243, 265, 273
BspANI GGCC 1 cut(s) 9
BspLI GGNNCC 2 cut(s) 321, 407
BspT107I GGYRCC 1 cut(s) 405
BspTNI GGTCTC 1 cut(s) 348
BssECI CCNNGG 1 cut(s) 112
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 351
BstC8I GCNNGC 2 cut(s) 7, 265
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 2 cut(s) 39, 219
BstH2I RGCGCY 2 cut(s) 255, 361
BstHHI GCGC 2 cut(s) 254, 360
BstMAI GTCTC 1 cut(s) 348
BstMWI GCNNNNNNNGC 3 cut(s) 106, 251, 260
BstSCI CCNGG 1 cut(s) 353
BstV1I GCAGC 1 cut(s) 347
BstXI CCANNNNNNTGG 1 cut(s) 30
BsuRI GGCC 1 cut(s) 9
BtsCI GGATG 2 cut(s) 39, 219
Cac8I GCNNGC 2 cut(s) 7, 265
CfoI GCGC 2 cut(s) 254, 360
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 406
CviQI GTAC 1 cut(s) 406
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
EciI GGCGGA 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 178
EcoICRI GAGCTC 1 cut(s) 178
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 112
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 112
FaiI YATR 3 cut(s) 132, 164, 376
FauI CCCGC 2 cut(s) 49, 272
Fnu4HI GCNGC 2 cut(s) 243, 361
FokI GGATG 2 cut(s) 46, 206
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 2 cut(s) 243, 361
FspBI CTAG 1 cut(s) 284
GlaI GCGC 2 cut(s) 253, 359
GluI GCNGC 2 cut(s) 243, 361
GsaI CCCAGC 2 cut(s) 26, 85
GsuI CTGGAG 1 cut(s) 122
HaeII RGCGCY 2 cut(s) 255, 361
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 355
HgaI GACGC 1 cut(s) 359
HhaI GCGC 2 cut(s) 254, 360
Hin1I GRCGYC 1 cut(s) 351
Hin6I GCGC 2 cut(s) 252, 358
HinP1I GCGC 2 cut(s) 252, 358
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 1 cut(s) 137
HpaII CCGG 1 cut(s) 355
HphI GGTGA 1 cut(s) 199
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 3 cut(s) 313, 347, 395
Hpy99I CGWCG 2 cut(s) 126, 353
HpyAV CCTTC 1 cut(s) 319
HpyCH4IV ACGT 2 cut(s) 121, 294
HpyCH4V TGCA 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 251, 260
HpySE526I ACGT 2 cut(s) 121, 294
Hsp92I GRCGYC 1 cut(s) 351
HspAI GCGC 2 cut(s) 252, 358
KpnI GGTACC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 4 cut(s) 36, 86, 95, 368
Lsp1109I GCAGC 1 cut(s) 347
MaeI CTAG 1 cut(s) 284
MaeII ACGT 2 cut(s) 121, 294
MboII GAAGA 1 cut(s) 188
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 2 cut(s) 28, 203
MspI CCGG 1 cut(s) 355
MspR9I CCNGG 1 cut(s) 355
MwoI GCNNNNNNNGC 3 cut(s) 106, 251, 260
NciI CCSGG 1 cut(s) 355
NlaIV GGNNCC 2 cut(s) 321, 407
PfeI GAWTC 1 cut(s) 137
PkrI GCNGC 2 cut(s) 244, 362
Psp124BI GAGCTC 1 cut(s) 180
PspFI CCCAGC 2 cut(s) 22, 81
PspN4I GGNNCC 2 cut(s) 321, 407
RsaI GTAC 1 cut(s) 407
RsaNI GTAC 1 cut(s) 406
SacI GAGCTC 1 cut(s) 180
SatI GCNGC 2 cut(s) 243, 361
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 1 cut(s) 180
SetI ASST 8 cut(s) 28, 87, 124, 174, 180, 230, 297, 411
SmlI CTYRAG 1 cut(s) 173
SmoI CTYRAG 1 cut(s) 173
Sse9I AATT 2 cut(s) 28, 203
SsiI CCGC 6 cut(s) 56, 200, 210, 243, 265, 273
SspMI CTAG 1 cut(s) 284
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 112
TaiI ACGT 2 cut(s) 124, 297
TaqI TCGA 2 cut(s) 156, 348
TasI AATT 2 cut(s) 28, 203
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 137
TseI GCWGC 1 cut(s) 360
XagI CCTNNNNNAGG 1 cut(s) 313
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.