pycom13g00050

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Reverse (-)
36597 .. 38358
1762 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGCTCGATACAAAGGGGCCTGAGATTCGGACTGGATTCCTCAAGGGTGGAAAACCCATACAACTTAAGGAAGGGCAGGAAATTACTATTACTACTGATTACAGCATCAAGGGGGATGAAGAGATGATTTCCATGAGCTACAAGAAGCTGGCTGTGGATTTGAAGCCGAGAAATACCATTTTATGTGCAGACGGCACCATTACTCTCACTGTATTATCTTGTAATCCAGCAGCTGGTACAGTTAGGTGCTGCTGTGAGAACACTGCAATGCTGGGTGAAAGAAAAAATGTCAATCTTCCTGGTATTGTGGTCGATCTTCCCACACTGACGGAGAAGGATAAGGAAGATATTCTGGAATGGGGCGTGCCCAACAAGATTGATATGATTGCTCTTTCATTTGTACGCAAGGGATCAGATCTTGTTAATGTCCGTAAAGTTCTTGGACCTCATGCAAAGACCATACAATTGATGTCAAAGGTTGAGAACCAGGAGGGAGTTATCAACTTTGATGAGATATTGCGAGAGACAGATTCATTCATGGTTGCCCGTGGTGATCTTGGAATGGAAATCCCAGTTGAGAAAATTTTTCTGGCACAAAAGATGATGATATATAAGTGTAATCTTGTGGGCAAGCCCGTGGTCACTGCCACTCAGATGCTTGAATCCATCGATCAAGTCTCCACGGCCAACCCGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

233

Amino Acids

25.54

Weight (kDa)

5.42

Isoelectric Point (pI)

20.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 1 - 225 2.8e-92 Pyruvate kinase, barrel domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 233
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 1 cut(s) 582
AfaI GTAC 2 cut(s) 238, 402
AfiI CCNNNNNNNGG 1 cut(s) 233
AflII CTTAAG 1 cut(s) 65
AgsI TTSAA 2 cut(s) 163, 662
AjnI CCWGG 2 cut(s) 298, 486
AluBI AGCT 3 cut(s) 138, 148, 233
AluI AGCT 3 cut(s) 138, 148, 233
Alw26I GTCTC 2 cut(s) 518, 682
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 233
AoxI GGCC 2 cut(s) 17, 684
ApeKI GCWGC 2 cut(s) 230, 249
ApoI RAATTY 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 2 cut(s) 17, 443
AsuHPI GGTGA 2 cut(s) 287, 563
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 369
BanI GGYRCC 1 cut(s) 194
BbvI GCAGC 2 cut(s) 236, 242
BccI CCATC 1 cut(s) 674
BceAI ACGGC 1 cut(s) 208
BciT130I CCWGG 2 cut(s) 300, 488
BcoDI GTCTC 2 cut(s) 518, 682
BfrI CTTAAG 1 cut(s) 65
BglII AGATCT 1 cut(s) 415
BisI GCNGC 2 cut(s) 231, 250
BlsI GCNGC 2 cut(s) 232, 251
Bme1390I CCNGG 2 cut(s) 300, 488
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 2 cut(s) 17, 443
BmiI GGNNCC 2 cut(s) 18, 196
BmrFI CCNGG 2 cut(s) 300, 488
BmrI ACTGGG 1 cut(s) 566
BmsI GCATC 2 cut(s) 114, 645
BmuI ACTGGG 1 cut(s) 566
BpuEI CTTGAG 1 cut(s) 26
Bsa29I ATCGAT 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 547, 636, 681
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse1I ACTGG 2 cut(s) 37, 572
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 2 cut(s) 300, 488
BseCI ATCGAT 1 cut(s) 669
BseDI CCNNGG 3 cut(s) 547, 636, 681
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 12, 665
BseNI ACTGG 2 cut(s) 37, 572
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 2 cut(s) 236, 242
BseYI CCCAGC 1 cut(s) 271
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 2 cut(s) 19, 686
BshNI GGYRCC 1 cut(s) 194
BshVI ATCGAT 1 cut(s) 669
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 518, 682
BsnI GGCC 2 cut(s) 19, 686
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 5 cut(s) 313, 410, 415, 553, 670
BspANI GGCC 2 cut(s) 19, 686
BspCNI CTCAG 2 cut(s) 13, 664
BspDI ATCGAT 1 cut(s) 669
BspLI GGNNCC 2 cut(s) 18, 196
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 194
BspTI CTTAAG 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 273
BsrI ACTGG 2 cut(s) 37, 572
BssECI CCNNGG 3 cut(s) 547, 636, 681
BssMI GATC 5 cut(s) 313, 410, 415, 553, 670
Bst2UI CCWGG 2 cut(s) 300, 488
Bst4CI ACNGT 2 cut(s) 211, 241
Bst6I CTCTTC 1 cut(s) 114
BstAFI CTTAAG 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 150, 365, 632
BstDEI CTNAG 2 cut(s) 21, 651
BstDSI CCRYGG 3 cut(s) 547, 636, 681
BstF5I GGATG 1 cut(s) 121
BstKTI GATC 5 cut(s) 316, 413, 418, 556, 673
BstMAI GTCTC 2 cut(s) 518, 682
BstMBI GATC 5 cut(s) 313, 410, 415, 553, 670
BstNI CCWGG 2 cut(s) 300, 488
BstSCI CCNGG 2 cut(s) 298, 486
BstSLI GKGCMC 1 cut(s) 369
BstV1I GCAGC 2 cut(s) 236, 242
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
Bsu15I ATCGAT 1 cut(s) 669
BsuRI GGCC 2 cut(s) 19, 686
BsuTUI ATCGAT 1 cut(s) 669
BtgI CCRYGG 3 cut(s) 547, 636, 681
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 2 cut(s) 261, 642
BtsIMutI CAGTG 4 cut(s) 207, 261, 323, 642
Cac8I GCNNGC 3 cut(s) 150, 365, 632
CaiI CAGNNNCTG 1 cut(s) 233
Cfr13I GGNCC 2 cut(s) 17, 443
ClaI ATCGAT 1 cut(s) 669
Csp6I GTAC 2 cut(s) 237, 401
CviAII CATG 3 cut(s) 133, 449, 538
CviJI RGCY 8 cut(s) 19, 138, 148, 152, 166, 233, 634, 686
CviKI_1 RGCY 8 cut(s) 19, 138, 148, 152, 166, 233, 634, 686
CviQI GTAC 2 cut(s) 237, 401
DdeI CTNAG 2 cut(s) 21, 651
DpnI GATC 5 cut(s) 315, 412, 417, 555, 672
DpnII GATC 5 cut(s) 313, 410, 415, 553, 670
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 114
EarI CTCTTC 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 298, 486
FaeI CATG 3 cut(s) 136, 452, 541
FaiI YATR 9 cut(s) 59, 134, 184, 383, 450, 461, 539, 610, 612
FatI CATG 3 cut(s) 132, 448, 537
Fnu4HI GCNGC 2 cut(s) 231, 250
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 2 cut(s) 231, 250
GluI GCNGC 2 cut(s) 231, 250
GsaI CCCAGC 1 cut(s) 275
HaeIII GGCC 2 cut(s) 19, 686
Hin1II CATG 3 cut(s) 136, 452, 541
HinfI GANTC 4 cut(s) 25, 36, 530, 662
HphI GGTGA 2 cut(s) 287, 563
Hpy188I TCNGA 3 cut(s) 30, 415, 654
Hpy188III TCNNGA 1 cut(s) 353
HpyAV CCTTC 2 cut(s) 65, 328
HpyCH4III ACNGT 2 cut(s) 211, 241
HpyCH4V TGCA 3 cut(s) 188, 266, 452
HpyF3I CTNAG 2 cut(s) 21, 651
Hsp92II CATG 3 cut(s) 136, 452, 541
Kzo9I GATC 5 cut(s) 313, 410, 415, 553, 670
Lsp1109I GCAGC 2 cut(s) 236, 242
LweI GCATC 2 cut(s) 114, 645
MaeIII GTNAC 1 cut(s) 640
MalI GATC 5 cut(s) 315, 412, 417, 555, 672
MboI GATC 5 cut(s) 313, 410, 415, 553, 670
MboII GAAGA 4 cut(s) 131, 287, 308, 356
MfeI CAATTG 1 cut(s) 464
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 3 cut(s) 81, 464, 582
MnlI CCTC 3 cut(s) 50, 456, 484
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 2 cut(s) 66, 423
MslI CAYNNNNRTG 2 cut(s) 266, 653
MspA1I CMGCKG 1 cut(s) 233
MspCI CTTAAG 1 cut(s) 65
MspR9I CCNGG 2 cut(s) 300, 488
MunI CAATTG 1 cut(s) 464
MvaI CCWGG 2 cut(s) 300, 488
NdeII GATC 5 cut(s) 313, 410, 415, 553, 670
NlaIII CATG 3 cut(s) 136, 452, 541
NlaIV GGNNCC 2 cut(s) 18, 196
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 640
PcsI WCGNNNNNNNCGW 1 cut(s) 689
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 4 cut(s) 25, 36, 530, 662
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 2 cut(s) 232, 251
Psp6I CCWGG 2 cut(s) 298, 486
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 2 cut(s) 298, 486
PspN4I GGNNCC 2 cut(s) 18, 196
PspPI GGNCC 2 cut(s) 17, 443
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 238, 402
RsaNI GTAC 2 cut(s) 237, 401
RseI CAYNNNNRTG 2 cut(s) 266, 653
SaqAI TTAA 2 cut(s) 66, 423
SatI GCNGC 2 cut(s) 231, 250
Sau3AI GATC 5 cut(s) 313, 410, 415, 553, 670
Sau96I GGNCC 2 cut(s) 17, 443
ScrFI CCNGG 2 cut(s) 300, 488
SduI GDGCHC 1 cut(s) 369
SetI ASST 6 cut(s) 140, 150, 235, 248, 448, 480
SfaNI GCATC 2 cut(s) 114, 645
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 2 cut(s) 266, 653
SmlI CTYRAG 2 cut(s) 41, 65
SmoI CTYRAG 2 cut(s) 41, 65
Sse9I AATT 3 cut(s) 81, 464, 582
StyD4I CCNGG 2 cut(s) 298, 486
TaaI ACNGT 2 cut(s) 211, 241
TaqI TCGA 3 cut(s) 6, 312, 669
TasI AATT 3 cut(s) 81, 464, 582
TfiI GAWTC 4 cut(s) 25, 36, 530, 662
Tru1I TTAA 2 cut(s) 66, 423
Tru9I TTAA 2 cut(s) 66, 423
TscAI CASTG 4 cut(s) 214, 268, 330, 649
TseFI GTSAC 1 cut(s) 640
TseI GCWGC 2 cut(s) 230, 249
Tsp45I GTSAC 1 cut(s) 640
TspDTI ATGAA 4 cut(s) 132, 384, 522, 526
TspGWI ACGGA 2 cut(s) 344, 419
TspRI CASTG 4 cut(s) 214, 268, 330, 649
Van91I CCANNNNNTGG 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 582
XmnI GAANNNNTTC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.