pycom13g02230

Phytanoyl-CoA dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024685
Physical Location & Seq
Reverse (-)
1547363 .. 1549357
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 615 bp
ATGGGAATCGTCGGAAGTCTCAGTCCTGACCAGCTCCAGACCTTCCACTCCCAAGGTTATCTTGTGATGGAATCGTTTGCGAGCGCGGAGGACATCGAGGCCATGAGGAACCGAATGGACCAATTGCTCCAGGACTTTGACTGCTCTTCCACCGCCTCTATTTTCTCCACCACCAACCAGAAGTCTACTGACAATTACTTCTATGATAGCGTTGACAAAATTTCGTTTTTCTTCGAAGAGAAAGCTTTTGGGAGTGATGGAGAACTGCGACAGCCGAAGGAACTTTCGATTAACAAAGTTGGCCATGCATTACATGAGCTCGATCCAGTATTTAAAGGGTTCACCTTTTCTGAGAAAGTTTCAGGCTTGTTCATCTCCTTGGGTTACAGAAGGCCCATTGTCATTCAGTCTATGTACATTTTCAAGCAACCAGGAATCGGAGGTGAGGTAGTGCCGCATCAGGATAACTCATTTCTTTATACCGATCGATCAACTTGCACGGGGCTGTGGCTGGCTTTAGAAGATGCAACAATAACGAATGGCTGCCTATGGGCTATTCCTGGATCGCAAAAGAGTAGCATTCTCCTCCAACTCTCTCTATTTTTTCCTAAATAA

Protein Analysis

205

Amino Acids

22.84

Weight (kDa)

4.92

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhyH PF05721 15 - 192 2e-36 Phytanoyl-CoA dioxygenase (PhyH)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024685)

Species Orthologous Gene IDs
pyrus_communis pycom13g02230
rosa_rugosa Rorug04G0334500
rosa_samantha Rh4DG392600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 185
AccII CGCG 1 cut(s) 86
AciI CCGC 3 cut(s) 86, 153, 455
AclWI GGATC 2 cut(s) 317, 571
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 1 cut(s) 219
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 1 cut(s) 437
AgsI TTSAA 1 cut(s) 424
AjnI CCWGG 3 cut(s) 129, 430, 559
AluBI AGCT 3 cut(s) 34, 245, 319
AluI AGCT 3 cut(s) 34, 245, 319
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 1 cut(s) 23
AlwI GGATC 2 cut(s) 317, 571
AoxI GGCC 3 cut(s) 99, 301, 392
ApeKI GCWGC 1 cut(s) 543
ApoI RAATTY 1 cut(s) 219
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 2 cut(s) 118, 393
AsuHPI GGTGA 2 cut(s) 334, 455
AsuII TTCGAA 1 cut(s) 234
AvaII GGWCC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 303
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 1 cut(s) 530
BccI CCATC 2 cut(s) 61, 251
BcgI CGANNNNNNTGC 2 cut(s) 477, 511
BciT130I CCWGG 3 cut(s) 131, 432, 561
BcoDI GTCTC 1 cut(s) 23
BisI GCNGC 2 cut(s) 455, 544
BlsI GCNGC 2 cut(s) 456, 545
Bme1390I CCNGG 3 cut(s) 131, 432, 561
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 118, 393
BmiI GGNNCC 1 cut(s) 110
BmrFI CCNGG 3 cut(s) 131, 432, 561
BmsI GCATC 2 cut(s) 466, 514
BpmI CTGGAG 2 cut(s) 20, 113
Bpu14I TTCGAA 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 52, 378
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse1I ACTGG 1 cut(s) 326
BseBI CCWGG 3 cut(s) 131, 432, 561
BseCI ATCGAT 1 cut(s) 487
BseDI CCNNGG 2 cut(s) 52, 378
BseLI CCNNNNNNNGG 1 cut(s) 437
BseMII CTCAG 2 cut(s) 34, 342
BseNI ACTGG 1 cut(s) 326
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 530
Bsh1236I CGCG 1 cut(s) 86
Bsh1285I CGRYCG 1 cut(s) 487
BshFI GGCC 3 cut(s) 101, 303, 394
BshVI ATCGAT 1 cut(s) 487
BsiEI CGRYCG 1 cut(s) 487
BsiHKAI GWGCWC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 579
BsnI GGCC 3 cut(s) 101, 303, 394
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 4 cut(s) 322, 484, 488, 563
BspACI CCGC 3 cut(s) 86, 153, 455
BspANI GGCC 3 cut(s) 101, 303, 394
BspCNI CTCAG 2 cut(s) 33, 343
BspDI ATCGAT 1 cut(s) 487
BspFNI CGCG 1 cut(s) 86
BspLI GGNNCC 1 cut(s) 110
BspPI GGATC 2 cut(s) 317, 571
BspQI GCTCTTC 1 cut(s) 151
BspT104I TTCGAA 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 1 cut(s) 326
BssECI CCNNGG 2 cut(s) 52, 378
BssMI GATC 4 cut(s) 322, 484, 488, 563
BssT1I CCWWGG 2 cut(s) 52, 378
Bst2UI CCWGG 3 cut(s) 131, 432, 561
Bst6I CTCTTC 2 cut(s) 151, 231
BstAUI TGTACA 1 cut(s) 414
BstBI TTCGAA 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 82, 513
BstDEI CTNAG 2 cut(s) 20, 351
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 4 cut(s) 325, 487, 491, 566
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 4 cut(s) 322, 484, 488, 563
BstMCI CGRYCG 1 cut(s) 487
BstNI CCWGG 3 cut(s) 131, 432, 561
BstSCI CCNGG 3 cut(s) 129, 430, 559
BstUI CGCG 1 cut(s) 86
BstV1I GCAGC 1 cut(s) 530
Bsu15I ATCGAT 1 cut(s) 487
BsuRI GGCC 3 cut(s) 101, 303, 394
BsuTUI ATCGAT 1 cut(s) 487
Cac8I GCNNGC 2 cut(s) 82, 513
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 118, 393
ClaI ATCGAT 1 cut(s) 487
Csp6I GTAC 1 cut(s) 415
CviAII CATG 3 cut(s) 103, 305, 314
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 2 cut(s) 20, 351
DpnI GATC 4 cut(s) 324, 486, 490, 565
DpnII GATC 4 cut(s) 322, 484, 488, 563
DraI TTTAAA 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 2 cut(s) 151, 231
EarI CTCTTC 2 cut(s) 151, 231
Ecl136II GAGCTC 1 cut(s) 319
Eco130I CCWWGG 2 cut(s) 52, 378
Eco24I GRGCYC 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 118
Eco53kI GAGCTC 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 129, 430, 559
EcoT14I CCWWGG 2 cut(s) 52, 378
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 52, 378
FaeI CATG 3 cut(s) 106, 308, 317
FaiI YATR 7 cut(s) 104, 204, 306, 315, 413, 480, 550
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FatI CATG 3 cut(s) 102, 304, 313
FblI GTMKAC 1 cut(s) 185
Fnu4HI GCNGC 2 cut(s) 455, 544
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 2 cut(s) 455, 544
GlaI GCGC 1 cut(s) 85
GluI GCNGC 2 cut(s) 455, 544
GsuI CTGGAG 2 cut(s) 20, 113
HaeIII GGCC 3 cut(s) 101, 303, 394
HhaI GCGC 1 cut(s) 86
Hin1II CATG 3 cut(s) 106, 308, 317
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 243
HinfI GANTC 3 cut(s) 6, 71, 435
HphI GGTGA 2 cut(s) 334, 455
Hpy166II GTNNAC 3 cut(s) 186, 214, 342
Hpy188I TCNGA 3 cut(s) 14, 352, 440
Hpy188III TCNNGA 3 cut(s) 26, 37, 461
Hpy8I GTNNAC 3 cut(s) 186, 214, 342
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 3 cut(s) 52, 271, 384
HpyCH4V TGCA 3 cut(s) 308, 498, 527
HpyF3I CTNAG 2 cut(s) 20, 351
Hsp92II CATG 3 cut(s) 106, 308, 317
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 4 cut(s) 322, 484, 488, 563
LguI GCTCTTC 1 cut(s) 151
LmnI GCTCC 2 cut(s) 39, 132
Lsp1109I GCAGC 1 cut(s) 530
LweI GCATC 2 cut(s) 466, 514
MaeIII GTNAC 1 cut(s) 383
MalI GATC 4 cut(s) 324, 486, 490, 565
MboI GATC 4 cut(s) 322, 484, 488, 563
MboII GAAGA 4 cut(s) 138, 223, 248, 533
MfeI CAATTG 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 321
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 3 cut(s) 122, 193, 219
MluNI TGGCCA 1 cut(s) 303
MmeI TCCRAC 1 cut(s) 613
MnlI CCTC 7 cut(s) 82, 91, 99, 166, 434, 439, 596
Mox20I TGGCCA 1 cut(s) 303
Mph1103I ATGCAT 1 cut(s) 310
MscI TGGCCA 1 cut(s) 303
MseI TTAA 2 cut(s) 291, 333
Msp20I TGGCCA 1 cut(s) 303
MspR9I CCNGG 3 cut(s) 131, 432, 561
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 579
MvaI CCWGG 3 cut(s) 131, 432, 561
MvnI CGCG 1 cut(s) 86
NdeII GATC 4 cut(s) 322, 484, 488, 563
NlaIII CATG 3 cut(s) 106, 308, 317
NlaIV GGNNCC 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 310
NspV TTCGAA 1 cut(s) 234
PciSI GCTCTTC 1 cut(s) 151
PctI GAATGC 1 cut(s) 579
PfeI GAWTC 3 cut(s) 6, 71, 435
PfoI TCCNGGA 2 cut(s) 129, 559
PkrI GCNGC 2 cut(s) 456, 545
Ple19I CGATCG 1 cut(s) 487
Psp124BI GAGCTC 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 129, 430, 559
PspGI CCWGG 3 cut(s) 129, 430, 559
PspN4I GGNNCC 1 cut(s) 110
PspPI GGNCC 2 cut(s) 118, 393
PvuI CGATCG 1 cut(s) 487
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SacI GAGCTC 1 cut(s) 321
SapI GCTCTTC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 291, 333
SatI GCNGC 2 cut(s) 455, 544
Sau3AI GATC 4 cut(s) 322, 484, 488, 563
Sau96I GGNCC 2 cut(s) 118, 393
ScrFI CCNGG 3 cut(s) 131, 432, 561
SduI GDGCHC 1 cut(s) 321
SetI ASST 8 cut(s) 36, 44, 58, 247, 321, 347, 445, 450
SfaNI GCATC 2 cut(s) 466, 514
SfuI TTCGAA 1 cut(s) 234
SinI GGWCC 1 cut(s) 118
Sse9I AATT 3 cut(s) 122, 193, 219
SsiI CCGC 3 cut(s) 86, 153, 455
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 3 cut(s) 129, 430, 559
StyI CCWWGG 2 cut(s) 52, 378
TaqI TCGA 5 cut(s) 96, 234, 287, 321, 487
TasI AATT 3 cut(s) 122, 193, 219
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 457
TfiI GAWTC 3 cut(s) 6, 71, 435
Tru1I TTAA 2 cut(s) 291, 333
Tru9I TTAA 2 cut(s) 291, 333
TseI GCWGC 1 cut(s) 543
TspDTI ATGAA 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 1 cut(s) 219
XmiI GTMKAC 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.