pycom13g04690

S-adenosylmethionine decarboxylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
3132012 .. 3132317
306 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 306 bp
ATGGCCATGGAGGGATCTGCTATTGGATTTGAAGGTTATGAAAAGAGGCTAGAAATAGCATTTTTCGAGCCGAGTGTCTTTCTCGACCCTGAAGGAAGGGGTCTTCGGTCTCTGTCCAAAGCTCAGATAGATGAATTTCTTGACCAAGCTGAGTGCACCATCGTCTCGTCATTGTCGAATGACGATGTTGACTCATATGTTCTTTCTGAGTCAAGCCTCTTTATTCATCCATACAAGATGATCGATCATAACTTGTGGCACAACCAAGTTGCTCCGTGTGATCCCGACTGTCCTGCAGTTGGCTGA

Protein Analysis

102

Amino Acids

11.24

Weight (kDa)

4.26

Isoelectric Point (pI)

57.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAM_decarbox PF01536 9 - 81 1.2e-25 Adenosylmethionine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 22, 275
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 111
AfiI CCNNNNNNNGG 1 cut(s) 299
AgsI TTSAA 1 cut(s) 32
AluBI AGCT 2 cut(s) 122, 149
AluI AGCT 2 cut(s) 122, 149
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 114, 169
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 2 cut(s) 22, 275
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 154
ApoI RAATTY 1 cut(s) 134
BaeGI GKGCMC 1 cut(s) 158
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 95
Bbv12I GWGCWC 1 cut(s) 158
BccI CCATC 1 cut(s) 167
BcgI CGANNNNNNTGC 1 cut(s) 275
BcoDI GTCTC 2 cut(s) 114, 169
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 95
Bsa29I ATCGAT 1 cut(s) 243
BsaI GGTCTC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 6
Bsc4I CCNNNNNNNGG 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 1 cut(s) 299
BseMII CTCAG 3 cut(s) 137, 141, 198
BseSI GKGCMC 1 cut(s) 158
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 299
BsmAI GTCTC 2 cut(s) 114, 169
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 114
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 4 cut(s) 14, 240, 244, 280
Bsp19I CCATGG 1 cut(s) 6
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 136, 142, 199
BspDI ATCGAT 1 cut(s) 243
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 2 cut(s) 22, 275
BspTNI GGTCTC 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 6
BssMI GATC 4 cut(s) 14, 240, 244, 280
BssT1I CCWWGG 1 cut(s) 6
Bst4CI ACNGT 1 cut(s) 290
BstDEI CTNAG 3 cut(s) 123, 150, 207
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 226
BstKTI GATC 4 cut(s) 17, 243, 247, 283
BstMAI GTCTC 2 cut(s) 114, 169
BstMBI GATC 4 cut(s) 14, 240, 244, 280
BstSFI CTRYAG 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 158
BstV2I GAAGAC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 14
BstYI RGATCY 1 cut(s) 14
Bsu15I ATCGAT 1 cut(s) 243
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 243
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 243
CviAII CATG 1 cut(s) 7
CviJI RGCY 7 cut(s) 5, 49, 70, 122, 149, 216, 303
CviKI_1 RGCY 7 cut(s) 5, 49, 70, 122, 149, 216, 303
DdeI CTNAG 3 cut(s) 123, 150, 207
DpnI GATC 4 cut(s) 16, 242, 246, 282
DpnII GATC 4 cut(s) 14, 240, 244, 280
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 6
Eco31I GGTCTC 1 cut(s) 114
Eco57I CTGAAG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 1 cut(s) 10
FaiI YATR 6 cut(s) 8, 39, 196, 198, 232, 249
FatI CATG 1 cut(s) 6
FauNDI CATATG 1 cut(s) 196
FokI GGATG 1 cut(s) 213
FspBI CTAG 1 cut(s) 50
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 10
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 2 cut(s) 191, 209
Hpy166II GTNNAC 2 cut(s) 156, 190
Hpy188I TCNGA 2 cut(s) 126, 208
Hpy188III TCNNGA 3 cut(s) 83, 140, 284
Hpy8I GTNNAC 2 cut(s) 156, 190
HpyAV CCTTC 3 cut(s) 26, 86, 90
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 2 cut(s) 156, 296
HpyF3I CTNAG 3 cut(s) 123, 150, 207
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 4 cut(s) 14, 240, 244, 280
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 1 cut(s) 102
MaeI CTAG 1 cut(s) 50
MalI GATC 4 cut(s) 16, 242, 246, 282
MboI GATC 4 cut(s) 14, 240, 244, 280
MboII GAAGA 1 cut(s) 95
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 185, 218
MnlI CCTC 3 cut(s) 4, 39, 227
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 196
NdeII GATC 4 cut(s) 14, 240, 244, 280
NlaIII CATG 1 cut(s) 10
NmeAIII GCCGAG 1 cut(s) 96
PcsI WCGNNNNNNNCGW 1 cut(s) 173
PleI GAGTC 2 cut(s) 185, 217
PpsI GAGTC 2 cut(s) 185, 217
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 1 cut(s) 14
Sau3AI GATC 4 cut(s) 14, 240, 244, 280
SchI GAGTC 2 cut(s) 185, 218
SduI GDGCHC 1 cut(s) 158
SetI ASST 3 cut(s) 37, 124, 151
SfcI CTRYAG 1 cut(s) 294
Sse9I AATT 1 cut(s) 134
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 4 cut(s) 66, 84, 176, 243
TaqII GACCGA 1 cut(s) 96
TasI AATT 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 54, 147, 215
TspGWI ACGGA 1 cut(s) 264
VneI GTGCAC 1 cut(s) 154
XapI RAATTY 1 cut(s) 134
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.