pycom13g05670

COBW domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Reverse (-)
3721971 .. 3723738
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGAAGGTGGATCCTAAACCTGTGAATTTCCCGGGATTGGAAAGGAAATGGAAGACGATGAAGAAGCTCCACCTCTCGCCATTGAAATCGACCAACCGCTGGGACCCTACTCTCAGCAGCAACCCAATTCATCTAGTCACAAAGACGACGACGTGTTGGTCGGCGTCACCGTCATCACCGGCTACCTCGGCGCCGGAAAATCCACCACTTGATCGTATATTGCTGGAGACTACTGGGTTAGCGAACCCCGTTCTACTTGCTTCGGTTCTGTGGTTGGATGATAATCTAGTATCATCTGTCATGCTTGATTCAATTATCACGAGGGTTCTGCGGATTTTCTGGAGGAACTGGAGAAGGAAATACATAGTATTAACTCACTCGCTAGTATCATCCGTTCTGTCCGTGGTCAAGTTGACCTGTCCAATATATTGGACTGTCGGGCCTATGATGCCACGCATGCTACCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CobW_C cobW

Protein Analysis

157

Amino Acids

17.64

Weight (kDa)

10.15

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 157
AccB1I GGYRCC 1 cut(s) 190
AccB7I CCANNNNNTGG 1 cut(s) 99
AciI CCGC 2 cut(s) 97, 331
AclWI GGATC 2 cut(s) 5, 18
AcsI RAATTY 1 cut(s) 25
AcyI GRCGYC 2 cut(s) 164, 191
AfiI CCNNNNNNNGG 2 cut(s) 37, 99
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 2 cut(s) 85, 312
AjiI CACGTC 1 cut(s) 153
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 2 cut(s) 5, 18
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 1 cut(s) 440
ApeKI GCWGC 1 cut(s) 117
ApoI RAATTY 1 cut(s) 25
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 103, 440
AsuC2I CCSGG 2 cut(s) 32, 33
AsuHPI GGTGA 2 cut(s) 159, 168
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 1 cut(s) 103
BamHI GGATCC 1 cut(s) 10
BanI GGYRCC 1 cut(s) 190
BauI CACGAG 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 59
BbvI GCAGC 1 cut(s) 129
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BcnI CCSGG 2 cut(s) 32, 33
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 3 cut(s) 134, 287, 383
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 32, 33
Bme18I GGWCC 1 cut(s) 103
BmeT110I CYCGRG 1 cut(s) 31
BmgBI CACGTC 1 cut(s) 153
BmgT120I GGNCC 2 cut(s) 103, 440
BmiI GGNNCC 4 cut(s) 12, 104, 105, 192
BmrFI CCNGG 2 cut(s) 32, 33
BmrI ACTGGG 2 cut(s) 243, 459
BmsI GCATC 1 cut(s) 438
BmuI ACTGGG 2 cut(s) 243, 459
BpiI GAAGAC 1 cut(s) 59
BpmI CTGGAG 3 cut(s) 245, 361, 370
BpuMI CCSGG 2 cut(s) 32, 33
BsaBI GATNNNNATC 1 cut(s) 282
BsaHI GRCGYC 2 cut(s) 164, 191
BsaJI CCNNGG 3 cut(s) 31, 186, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 99
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 3 cut(s) 238, 353, 465
Bse8I GATNNNNATC 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 31, 186, 402
BseGI GGATG 2 cut(s) 283, 389
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 2 cut(s) 37, 99
BseMII CTCAG 1 cut(s) 127
BseNI ACTGG 3 cut(s) 238, 353, 465
BseXI GCAGC 1 cut(s) 129
BseYI CCCAGC 1 cut(s) 99
BshFI GGCC 1 cut(s) 442
BshNI GGYRCC 1 cut(s) 190
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 3 cut(s) 32, 179, 194
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 37, 99
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 116
BsnI GGCC 1 cut(s) 442
BsoBI CYCGRG 1 cut(s) 31
Bsp143I GATC 2 cut(s) 10, 211
BspACI CCGC 2 cut(s) 97, 331
BspANI GGCC 1 cut(s) 442
BspCNI CTCAG 1 cut(s) 126
BspLI GGNNCC 4 cut(s) 12, 104, 105, 192
BspPI GGATC 2 cut(s) 5, 18
BspT107I GGYRCC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 3 cut(s) 238, 353, 465
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 31, 186, 402
BssMI GATC 2 cut(s) 10, 211
BssNI GRCGYC 2 cut(s) 164, 191
BssSI CACGAG 1 cut(s) 319
Bst2BI CACGAG 1 cut(s) 319
Bst4CI ACNGT 2 cut(s) 171, 436
BstACI GRCGYC 2 cut(s) 164, 191
BstC8I GCNNGC 1 cut(s) 458
BstDEI CTNAG 1 cut(s) 113
BstDSI CCRYGG 1 cut(s) 402
BstF5I GGATG 2 cut(s) 283, 389
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 2 cut(s) 13, 214
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 10, 211
BstMWI GCNNNNNNNGC 3 cut(s) 188, 448, 457
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 2 cut(s) 30, 31
BstV1I GCAGC 1 cut(s) 129
BstV2I GAAGAC 1 cut(s) 59
BstX2I RGATCY 1 cut(s) 10
BstXI CCANNNNNNTGG 1 cut(s) 429
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 1 cut(s) 442
BtgI CCRYGG 1 cut(s) 402
BtrI CACGTC 1 cut(s) 153
BtsCI GGATG 2 cut(s) 283, 389
Cac8I GCNNGC 1 cut(s) 458
CfoI GCGC 1 cut(s) 193
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 2 cut(s) 103, 440
Cfr9I CCCGGG 1 cut(s) 31
CseI GACGC 1 cut(s) 153
CviAII CATG 2 cut(s) 301, 457
CviJI RGCY 3 cut(s) 67, 182, 442
CviKI_1 RGCY 3 cut(s) 67, 182, 442
DdeI CTNAG 1 cut(s) 113
DinI GGCGCC 1 cut(s) 192
DpnI GATC 2 cut(s) 12, 213
DpnII GATC 2 cut(s) 10, 211
DrdI GACNNNNNNGTC 1 cut(s) 157
DseDI GACNNNNNNGTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 103
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
FaeI CATG 2 cut(s) 304, 460
FaiI YATR 6 cut(s) 218, 302, 365, 427, 446, 458
FaqI GGGAC 1 cut(s) 116
FatI CATG 2 cut(s) 300, 456
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 2 cut(s) 290, 376
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 3 cut(s) 134, 287, 383
GlaI GCGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 118
GsaI CCCAGC 1 cut(s) 103
GsuI CTGGAG 3 cut(s) 245, 361, 370
HaeII RGCGCY 1 cut(s) 194
HaeIII GGCC 1 cut(s) 442
HapII CCGG 3 cut(s) 32, 179, 194
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 2 cut(s) 164, 191
Hin1II CATG 2 cut(s) 304, 460
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HinfI GANTC 1 cut(s) 308
HpaII CCGG 3 cut(s) 32, 179, 194
HphI GGTGA 2 cut(s) 159, 168
Hpy166II GTNNAC 1 cut(s) 414
Hpy188III TCNNGA 2 cut(s) 319, 340
Hpy8I GTNNAC 1 cut(s) 414
Hpy99I CGWCG 2 cut(s) 151, 154
HpyAV CCTTC 1 cut(s) 348
HpyCH4III ACNGT 2 cut(s) 171, 436
HpyCH4IV ACGT 1 cut(s) 152
HpyF10VI GCNNNNNNNGC 3 cut(s) 188, 448, 457
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 152
Hsp92I GRCGYC 2 cut(s) 164, 191
Hsp92II CATG 2 cut(s) 304, 460
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
KflI GGGWCCC 1 cut(s) 103
Kzo9I GATC 2 cut(s) 10, 211
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 1 cut(s) 129
LweI GCATC 1 cut(s) 438
MaeI CTAG 3 cut(s) 134, 287, 383
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 2 cut(s) 136, 165
MalI GATC 2 cut(s) 12, 213
MboI GATC 2 cut(s) 10, 211
MboII GAAGA 2 cut(s) 64, 73
MflI RGATCY 1 cut(s) 10
MluCI AATT 3 cut(s) 25, 126, 312
Mly113I GGCGCC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 4 cut(s) 83, 196, 315, 336
MseI TTAA 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 99
MspI CCGG 3 cut(s) 32, 179, 194
MspR9I CCNGG 2 cut(s) 32, 33
MwoI GCNNNNNNNGC 3 cut(s) 188, 448, 457
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 2 cut(s) 32, 33
NdeII GATC 2 cut(s) 10, 211
NlaIII CATG 2 cut(s) 304, 460
NlaIV GGNNCC 4 cut(s) 12, 104, 105, 192
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 2 cut(s) 136, 165
NspI RCATGY 1 cut(s) 460
PaeI GCATGC 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 99
PkrI GCNGC 1 cut(s) 119
PluTI GGCGCC 1 cut(s) 194
PpuMI RGGWCCY 1 cut(s) 103
Psp5II RGGWCCY 1 cut(s) 103
PspFI CCCAGC 1 cut(s) 99
PspN4I GGNNCC 4 cut(s) 12, 104, 105, 192
PspPI GGNCC 2 cut(s) 103, 440
PspPPI RGGWCCY 1 cut(s) 103
PsrI GAACNNNNNNTAC 2 cut(s) 378, 410
PsuI RGATCY 1 cut(s) 10
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 2 cut(s) 10, 211
Sau96I GGNCC 2 cut(s) 103, 440
ScrFI CCNGG 2 cut(s) 32, 33
SetI ASST 7 cut(s) 9, 22, 69, 75, 155, 188, 419
SfaNI GCATC 1 cut(s) 438
SfoI GGCGCC 1 cut(s) 192
SinI GGWCC 1 cut(s) 103
SmaI CCCGGG 1 cut(s) 33
SphI GCATGC 1 cut(s) 460
Sse9I AATT 3 cut(s) 25, 126, 312
SsiI CCGC 2 cut(s) 97, 331
SspDI GGCGCC 1 cut(s) 190
SspMI CTAG 3 cut(s) 134, 287, 383
StyD4I CCNGG 2 cut(s) 30, 31
TaaI ACNGT 2 cut(s) 171, 436
TaiI ACGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 89
TasI AATT 3 cut(s) 25, 126, 312
TfiI GAWTC 1 cut(s) 308
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseFI GTSAC 2 cut(s) 136, 165
TseI GCWGC 1 cut(s) 117
Tsp45I GTSAC 2 cut(s) 136, 165
TspDTI ATGAA 3 cut(s) 17, 74, 119
TspGWI ACGGA 2 cut(s) 382, 391
TspMI CCCGGG 1 cut(s) 31
Van91I CCANNNNNTGG 1 cut(s) 99
VpaK11BI GGWCC 1 cut(s) 103
XapI RAATTY 1 cut(s) 25
XceI RCATGY 1 cut(s) 460
XmaI CCCGGG 1 cut(s) 31
XspI CTAG 3 cut(s) 134, 287, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.