pycom13g10510

endoplasmic reticulum protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Reverse (-)
7031098 .. 7032491
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
ATGTGTGTTAAAAATTCATTCGGTGAAGATCCGGTTTCCGGGATACACGCACTTACCCCTAATCACCACCCGACGCGTGTCCCGAATTGCGTGGTTTGGCCAATCGCAGCGAAGGCGGTGACTCCGTCACGCAGCGGTCGGGGACCCTATCATCCGTTTGCCAAAGATAAAACCGTCAGAAAACGCTACTGTTTTCGCGGAAGAGAAACTAAGGGCGAGAGTCAGAACTCGCCTCAGAGAGAGAATTCGACCAGGAAGGGGAGGAGTAATCACCTGCGACGCACTTCAGCTACCGGATCTACACCCGCAACCATGACGACTTCAAGGCGTTTGGCTGACAGGAAGGTGGAGAGGTTCGAGAAGAACATAACGAAGAGAGGGGCGGTGCCTGAGACGACCGCCAAGAAGAGCAAGGATTATCCAGTGGGCCCGATTCTCCTTGGCTTCTTCGTCTTTGTTGTCATCGGATCATCTCTCTTTCAGATAATCAGGACTGCTACAAGCGGAGGCATGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RAMP4

Protein Analysis

173

Amino Acids

18.98

Weight (kDa)

10.98

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RAMP4 PF06624 109 - 165 1.1e-25 Ribosome associated membrane protein RAMP4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 385
AccII CGCG 2 cut(s) 76, 198
AciI CCGC 7 cut(s) 116, 135, 198, 306, 383, 399, 504
AclWI GGATC 3 cut(s) 23, 304, 475
AcoI YGGCCR 1 cut(s) 98
AcsI RAATTY 2 cut(s) 13, 244
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 2 cut(s) 38, 258
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 1 cut(s) 324
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 3 cut(s) 23, 304, 475
AoxI GGCC 3 cut(s) 98, 427, 513
ApaI GGGCCC 1 cut(s) 431
ApeKI GCWGC 2 cut(s) 107, 132
ApoI RAATTY 2 cut(s) 13, 244
AspS9I GGNCC 3 cut(s) 143, 427, 428
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 4 cut(s) 35, 56, 130, 263
AvaII GGWCC 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 431
BalI TGGCCA 1 cut(s) 100
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 119, 144
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 1 cut(s) 36
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 282
BfuI GTATCC 1 cut(s) 36
BisI GCNGC 2 cut(s) 108, 133
BlsI GCNGC 2 cut(s) 109, 134
Bme1390I CCNGG 2 cut(s) 40, 253
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 3 cut(s) 143, 427, 428
BmiI GGNNCC 4 cut(s) 144, 145, 387, 429
BmrFI CCNGG 2 cut(s) 40, 253
BoxI GACNNNNGTC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 439
BsaWI WCCGGW 2 cut(s) 31, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 258
Bse1I ACTGG 1 cut(s) 422
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 1 cut(s) 151
BseLI CCNNNNNNNGG 2 cut(s) 38, 258
BseMII CTCAG 2 cut(s) 248, 381
BseNI ACTGG 1 cut(s) 422
BseRI GAGGAG 1 cut(s) 277
BseSI GKGCMC 1 cut(s) 431
BseXI GCAGC 2 cut(s) 119, 144
Bsh1236I CGCG 2 cut(s) 76, 198
Bsh1285I CGRYCG 2 cut(s) 139, 399
BshFI GGCC 3 cut(s) 100, 429, 515
BshNI GGYRCC 1 cut(s) 385
BsiEI CGRYCG 2 cut(s) 139, 399
BsiSI CCGG 3 cut(s) 32, 39, 294
BslFI GGGAC 2 cut(s) 65, 156
BslI CCNNNNNNNGG 2 cut(s) 38, 258
BsmAI GTCTC 1 cut(s) 386
BsmBI CGTCTC 1 cut(s) 386
BsmFI GGGAC 2 cut(s) 65, 156
BsnI GGCC 3 cut(s) 100, 429, 515
Bsp120I GGGCCC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 3 cut(s) 28, 296, 467
BspACI CCGC 7 cut(s) 116, 135, 198, 306, 383, 399, 504
BspANI GGCC 3 cut(s) 100, 429, 515
BspCNI CTCAG 2 cut(s) 247, 382
BspFNI CGCG 2 cut(s) 76, 198
BspLI GGNNCC 4 cut(s) 144, 145, 387, 429
BspMI ACCTGC 1 cut(s) 282
BspPI GGATC 3 cut(s) 23, 304, 475
BspQI GCTCTTC 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 385
BsrI ACTGG 1 cut(s) 422
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 3 cut(s) 28, 296, 467
BssT1I CCWWGG 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 175, 191
Bst6I CTCTTC 3 cut(s) 196, 368, 401
BstDEI CTNAG 3 cut(s) 210, 234, 390
BstF5I GGATG 1 cut(s) 151
BstFNI CGCG 2 cut(s) 76, 198
BstKTI GATC 3 cut(s) 31, 299, 470
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 3 cut(s) 28, 296, 467
BstMCI CGRYCG 2 cut(s) 139, 399
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 1 cut(s) 253
BstPAI GACNNNNGTC 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 38, 251
BstSLI GKGCMC 1 cut(s) 431
BstUI CGCG 2 cut(s) 76, 198
BstV1I GCAGC 2 cut(s) 119, 144
BstX2I RGATCY 2 cut(s) 28, 296
BstYI RGATCY 2 cut(s) 28, 296
BsuI GTATCC 1 cut(s) 36
BsuRI GGCC 3 cut(s) 100, 429, 515
BtsCI GGATG 1 cut(s) 151
BtsIMutI CAGTG 1 cut(s) 429
BveI ACCTGC 1 cut(s) 282
Cfr13I GGNCC 3 cut(s) 143, 427, 428
CseI GACGC 2 cut(s) 82, 288
CviAII CATG 2 cut(s) 313, 511
CviJI RGCY 6 cut(s) 100, 290, 335, 429, 444, 515
CviKI_1 RGCY 6 cut(s) 100, 290, 335, 429, 444, 515
DdeI CTNAG 3 cut(s) 210, 234, 390
DpnI GATC 3 cut(s) 30, 298, 469
DpnII GATC 3 cut(s) 28, 296, 467
EaeI YGGCCR 1 cut(s) 98
Eam1104I CTCTTC 3 cut(s) 196, 368, 401
EarI CTCTTC 3 cut(s) 196, 368, 401
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 143
Eco57I CTGAAG 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 143
EcoRI GAATTC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 439
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 2 cut(s) 316, 514
FaiI YATR 3 cut(s) 314, 368, 512
FaqI GGGAC 2 cut(s) 65, 156
FatI CATG 2 cut(s) 312, 510
FauI CCCGC 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 108, 133
FokI GGATG 1 cut(s) 138
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 2 cut(s) 108, 133
GluI GCNGC 2 cut(s) 108, 133
HaeIII GGCC 3 cut(s) 100, 429, 515
HapII CCGG 3 cut(s) 32, 39, 294
HgaI GACGC 2 cut(s) 82, 288
Hin1II CATG 2 cut(s) 316, 514
HinfI GANTC 3 cut(s) 121, 220, 433
HpaII CCGG 3 cut(s) 32, 39, 294
HphI GGTGA 4 cut(s) 35, 56, 130, 263
Hpy188I TCNGA 5 cut(s) 179, 225, 237, 467, 483
Hpy188III TCNNGA 3 cut(s) 82, 358, 490
Hpy99I CGWCG 2 cut(s) 76, 282
HpyAV CCTTC 3 cut(s) 106, 250, 337
HpyCH4III ACNGT 2 cut(s) 175, 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 3 cut(s) 210, 234, 390
Hsp92II CATG 2 cut(s) 316, 514
KflI GGGWCCC 1 cut(s) 143
Kzo9I GATC 3 cut(s) 28, 296, 467
LguI GCTCTTC 1 cut(s) 401
Lsp1109I GCAGC 2 cut(s) 119, 144
MaeIII GTNAC 2 cut(s) 118, 126
MalI GATC 3 cut(s) 30, 298, 469
MboI GATC 3 cut(s) 28, 296, 467
MboII GAAGA 6 cut(s) 38, 213, 373, 385, 418, 439
MflI RGATCY 2 cut(s) 28, 296
MhlI GDGCHC 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 3 cut(s) 13, 85, 244
MluI ACGCGT 1 cut(s) 74
MluNI TGGCCA 1 cut(s) 100
MlyI GAGTC 2 cut(s) 115, 229
MnlI CCTC 5 cut(s) 243, 255, 345, 371, 500
Mox20I TGGCCA 1 cut(s) 100
MscI TGGCCA 1 cut(s) 100
MseI TTAA 1 cut(s) 9
Msp20I TGGCCA 1 cut(s) 100
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 3 cut(s) 32, 39, 294
MspR9I CCNGG 2 cut(s) 40, 253
MvaI CCWGG 1 cut(s) 253
MvnI CGCG 2 cut(s) 76, 198
MwoI GCNNNNNNNGC 1 cut(s) 113
NciI CCSGG 1 cut(s) 40
NdeII GATC 3 cut(s) 28, 296, 467
NlaIII CATG 2 cut(s) 316, 514
NlaIV GGNNCC 4 cut(s) 144, 145, 387, 429
NmuCI GTSAC 2 cut(s) 118, 126
PaqCI CACCTGC 1 cut(s) 282
PciSI GCTCTTC 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 1 cut(s) 433
PflFI GACNNNGTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 38
PkrI GCNGC 2 cut(s) 109, 134
PleI GAGTC 2 cut(s) 115, 228
PpsI GAGTC 2 cut(s) 115, 228
PpuMI RGGWCCY 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 143
Psp6I CCWGG 1 cut(s) 251
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 4 cut(s) 144, 145, 387, 429
PspOMI GGGCCC 1 cut(s) 427
PspPI GGNCC 3 cut(s) 143, 427, 428
PspPPI RGGWCCY 1 cut(s) 143
PsuI RGATCY 2 cut(s) 28, 296
PsyI GACNNNGTC 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 401
SaqAI TTAA 1 cut(s) 9
SatI GCNGC 2 cut(s) 108, 133
Sau3AI GATC 3 cut(s) 28, 296, 467
Sau96I GGNCC 3 cut(s) 143, 427, 428
SchI GAGTC 2 cut(s) 115, 229
ScrFI CCNGG 2 cut(s) 40, 253
SduI GDGCHC 1 cut(s) 431
SetI ASST 4 cut(s) 276, 292, 348, 356
SinI GGWCC 1 cut(s) 143
Sse9I AATT 3 cut(s) 13, 85, 244
SsiI CCGC 7 cut(s) 116, 135, 198, 306, 383, 399, 504
StyD4I CCNGG 2 cut(s) 38, 251
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 2 cut(s) 175, 191
TaqI TCGA 2 cut(s) 248, 357
TasI AATT 3 cut(s) 13, 85, 244
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 9
Tru9I TTAA 1 cut(s) 9
TscAI CASTG 1 cut(s) 429
TseFI GTSAC 2 cut(s) 118, 126
TseI GCWGC 2 cut(s) 107, 132
Tsp45I GTSAC 2 cut(s) 118, 126
TspDTI ATGAA 1 cut(s) 6
TspGWI ACGGA 2 cut(s) 114, 144
TspRI CASTG 1 cut(s) 429
Tth111I GACNNNGTC 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 2 cut(s) 13, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.