pycom13g12190

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
8423325 .. 8424599
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGGTAGTCCCGCAGTTGTAGTAGGCCTTCCTTCTCCTCCAAATCTTAACGGCTCTCTCTGTCCTCCAATTCCCACAACTTCTATAAGCAGCTCGAAGAAAAAGGATAGAATTGGAGAGCAAATGGTAAGGAAGCAGACAGGGGATGGTGGGTACAAGTGGCGTTTGGTGATTGCTTATGACGGCACTCGTTATGCAGGATGGCAATACCAGAGTTCACCGCCTACAATACAGTGCTTTGTGGAGAATGCTATAATGAGCGCAACGAAACTGGAAAGGAAGGATCTTCATTTCGTTGGTGCAAGTAGGACGGATAAAGGAGTACATGCCTGGGGTCAGGTTGCACACTTTGTCACACCTTTCAACTATGACAGCATTGAAAGCATTCATGCTGCTCTTAACGGTCTTCTTCCTGATGATATCCGAGTTAGGGAGATCGAACCCTGCAGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

152

Amino Acids

16.5

Weight (kDa)

8.46

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 58 - 149 5.4e-08 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 12, 221
AclWI GGATC 1 cut(s) 291
AfaI GTAC 2 cut(s) 155, 324
AfiI CCNNNNNNNGG 1 cut(s) 430
AgsI TTSAA 2 cut(s) 364, 380
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 1 cut(s) 93
AluI AGCT 1 cut(s) 93
AlwI GGATC 1 cut(s) 291
AlwNI CAGNNNCTG 1 cut(s) 453
AoxI GGCC 1 cut(s) 25
ApeKI GCWGC 2 cut(s) 90, 392
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 181, 210
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbsI GAAGAC 1 cut(s) 398
BbvI GCAGC 2 cut(s) 102, 379
BccI CCATC 2 cut(s) 140, 195
BceAI ACGGC 2 cut(s) 67, 199
BciT130I CCWGG 1 cut(s) 331
BfmI CTRYAG 1 cut(s) 445
BisI GCNGC 2 cut(s) 91, 393
BlsI GCNGC 2 cut(s) 92, 394
Bme1390I CCNGG 1 cut(s) 331
BmrFI CCNGG 1 cut(s) 331
BpiI GAAGAC 1 cut(s) 398
BsaJI CCNNGG 1 cut(s) 330
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 1 cut(s) 276
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 151, 206
BseLI CCNNNNNNNGG 1 cut(s) 430
BseNI ACTGG 1 cut(s) 276
BseRI GAGGAG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 102, 379
BshFI GGCC 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 430
BsmI GAATGC 2 cut(s) 253, 384
BsnI GGCC 1 cut(s) 27
Bsp143I GATC 2 cut(s) 283, 435
BspACI CCGC 2 cut(s) 12, 221
BspANI GGCC 1 cut(s) 27
BspMAI CTGCAG 1 cut(s) 449
BspPI GGATC 1 cut(s) 291
BsrI ACTGG 1 cut(s) 276
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 2 cut(s) 283, 435
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 2 cut(s) 234, 404
BstF5I GGATG 2 cut(s) 151, 206
BstHHI GCGC 1 cut(s) 263
BstKTI GATC 2 cut(s) 286, 438
BstMBI GATC 2 cut(s) 283, 435
BstMWI GCNNNNNNNGC 1 cut(s) 381
BstNI CCWGG 1 cut(s) 331
BstNSI RCATGY 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 329
BstSFI CTRYAG 1 cut(s) 445
BstV1I GCAGC 2 cut(s) 102, 379
BstV2I GAAGAC 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 283
BstYI RGATCY 1 cut(s) 283
BsuRI GGCC 1 cut(s) 27
BtsCI GGATG 2 cut(s) 151, 206
BtsI GCAGTG 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 239, 454
CaiI CAGNNNCTG 1 cut(s) 453
CfoI GCGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 154, 323
CviAII CATG 2 cut(s) 326, 389
CviJI RGCY 3 cut(s) 27, 54, 93
CviKI_1 RGCY 3 cut(s) 27, 54, 93
CviQI GTAC 2 cut(s) 154, 323
DpnI GATC 2 cut(s) 285, 437
DpnII GATC 2 cut(s) 283, 435
Eco147I AGGCCT 1 cut(s) 27
Eco32I GATATC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 329
EcoRV GATATC 1 cut(s) 421
FaeI CATG 2 cut(s) 329, 392
FaiI YATR 7 cut(s) 86, 180, 195, 254, 327, 369, 390
FatI CATG 2 cut(s) 325, 388
FauI CCCGC 1 cut(s) 19
Fnu4HI GCNGC 2 cut(s) 91, 393
FokI GGATG 2 cut(s) 158, 213
Fsp4HI GCNGC 2 cut(s) 91, 393
GlaI GCGC 1 cut(s) 262
GluI GCNGC 2 cut(s) 91, 393
HaeIII GGCC 1 cut(s) 27
HhaI GCGC 1 cut(s) 263
Hin1II CATG 2 cut(s) 329, 392
Hin6I GCGC 1 cut(s) 261
HinP1I GCGC 1 cut(s) 261
HphI GGTGA 2 cut(s) 181, 210
Hpy166II GTNNAC 1 cut(s) 218
Hpy188I TCNGA 1 cut(s) 425
Hpy188III TCNNGA 1 cut(s) 413
Hpy8I GTNNAC 1 cut(s) 218
HpyAV CCTTC 3 cut(s) 38, 42, 274
HpyCH4III ACNGT 2 cut(s) 234, 404
HpyCH4V TGCA 4 cut(s) 197, 302, 344, 447
HpyF10VI GCNNNNNNNGC 1 cut(s) 381
Hsp92II CATG 2 cut(s) 329, 392
HspAI GCGC 1 cut(s) 261
Kzo9I GATC 2 cut(s) 283, 435
LpnPI CCDG 8 cut(s) 126, 183, 224, 257, 316, 323, 343, 426
Lsp1109I GCAGC 2 cut(s) 102, 379
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 285, 437
MboI GATC 2 cut(s) 283, 435
MboII GAAGA 4 cut(s) 109, 278, 398, 401
MflI RGATCY 1 cut(s) 283
MluCI AATT 2 cut(s) 69, 111
MnlI CCTC 2 cut(s) 48, 75
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 2 cut(s) 48, 399
MspR9I CCNGG 1 cut(s) 331
Mva1269I GAATGC 2 cut(s) 253, 384
MvaI CCWGG 1 cut(s) 331
MwoI GCNNNNNNNGC 1 cut(s) 381
NdeII GATC 2 cut(s) 283, 435
NlaIII CATG 2 cut(s) 329, 392
NmuCI GTSAC 1 cut(s) 352
NspI RCATGY 1 cut(s) 329
PceI AGGCCT 1 cut(s) 27
PctI GAATGC 2 cut(s) 253, 384
PdmI GAANNNNTTC 1 cut(s) 384
PkrI GCNGC 2 cut(s) 92, 394
Psp6I CCWGG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 329
PstI CTGCAG 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 453
PsuI RGATCY 1 cut(s) 283
RsaI GTAC 2 cut(s) 155, 324
RsaNI GTAC 2 cut(s) 154, 323
SaqAI TTAA 2 cut(s) 48, 399
SatI GCNGC 2 cut(s) 91, 393
Sau3AI GATC 2 cut(s) 283, 435
ScrFI CCNGG 1 cut(s) 331
SetI ASST 3 cut(s) 95, 342, 361
SfcI CTRYAG 1 cut(s) 445
Sse9I AATT 2 cut(s) 69, 111
SseBI AGGCCT 1 cut(s) 27
SsiI CCGC 2 cut(s) 12, 221
StuI AGGCCT 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 329
TaaI ACNGT 2 cut(s) 234, 404
TaqI TCGA 2 cut(s) 95, 438
TasI AATT 2 cut(s) 69, 111
TatI WGTACW 1 cut(s) 322
Tru1I TTAA 2 cut(s) 48, 399
Tru9I TTAA 2 cut(s) 48, 399
TscAI CASTG 2 cut(s) 239, 454
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 90, 392
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 278, 377
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 2 cut(s) 239, 454
XceI RCATGY 1 cut(s) 329
XmnI GAANNNNTTC 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.