pycom13g19120

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
15165680 .. 15167438
1759 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 411 bp
ATGCCTTTCAAGAGGTACGTCGATCGATCGGAAGGGTGGCTCTCGTCAACTACGGAGAGGACTACGGGAAGCTCGTCGTCATCGTCGATGTCCTTGACCAGAACAGGAAGGGCTCTGGTTGATGCGCCTGATATGGTGAGGTCCCAAATGAATTTCAAGAGACTTTCTCTCACTGACATCAAAATCGACATCAAGAGGGTCCCCAAGAAGAAGGAACTGCTTGCTGCCATGGAGGCCGCTGATGTGAAGAAGAAGTGGGAGAACAGTTCGTGGGGTAGAAAACTGATTGTTCAGAAGAGAAGGGCGGCTCTTAATGACTTTGATCGGTTCAAGCTCATGCTGGCTAAGATTAAGAAAGCTGGGCTTGTCAGGAATGAGCTTGCGAAACTGAAGAAGCAGAGTGCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L14e

Protein Analysis

137

Amino Acids

15.48

Weight (kDa)

10.66

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14e PF01929 47 - 119 6.8e-26 Ribosomal protein L14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 237, 305
AcsI RAATTY 1 cut(s) 151
AcuI CTGAAG 1 cut(s) 410
AfaI GTAC 1 cut(s) 17
AgsI TTSAA 3 cut(s) 10, 157, 331
AluBI AGCT 5 cut(s) 72, 334, 359, 379, 407
AluI AGCT 5 cut(s) 72, 334, 359, 379, 407
Alw26I GTCTC 1 cut(s) 154
AoxI GGCC 1 cut(s) 234
ApeKI GCWGC 2 cut(s) 224, 404
ApoI RAATTY 1 cut(s) 151
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 141, 199
AsuHPI GGTGA 1 cut(s) 148
AvaII GGWCC 2 cut(s) 141, 199
BanII GRGCYC 1 cut(s) 115
BbvI GCAGC 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 154
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 4 cut(s) 225, 237, 306, 405
BlsI GCNGC 4 cut(s) 226, 238, 307, 406
Bme18I GGWCC 2 cut(s) 141, 199
BmgT120I GGNCC 2 cut(s) 141, 199
BmiI GGNNCC 3 cut(s) 143, 200, 201
BmsI GCATC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 151, 183
Bsa29I ATCGAT 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 228
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 1 cut(s) 359
Bsh1285I CGRYCG 2 cut(s) 25, 29
BshFI GGCC 1 cut(s) 236
BshVI ATCGAT 1 cut(s) 25
BsiEI CGRYCG 2 cut(s) 25, 29
BslFI GGGAC 2 cut(s) 127, 185
BsmAI GTCTC 1 cut(s) 154
BsmFI GGGAC 2 cut(s) 127, 185
BsnI GGCC 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 3 cut(s) 22, 26, 322
Bsp19I CCATGG 1 cut(s) 228
BspACI CCGC 2 cut(s) 237, 305
BspANI GGCC 1 cut(s) 236
BspDI ATCGAT 1 cut(s) 25
BspLI GGNNCC 3 cut(s) 143, 200, 201
BssECI CCNNGG 1 cut(s) 228
BssMI GATC 3 cut(s) 22, 26, 322
BssT1I CCWWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 266
Bst6I CTCTTC 1 cut(s) 290
BstC8I GCNNGC 3 cut(s) 222, 342, 381
BstDEI CTNAG 2 cut(s) 345, 408
BstDSI CCRYGG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 3 cut(s) 25, 29, 325
BstMAI GTCTC 1 cut(s) 154
BstMBI GATC 3 cut(s) 22, 26, 322
BstMCI CGRYCG 2 cut(s) 25, 29
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstV1I GCAGC 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 1 cut(s) 236
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 171
Cac8I GCNNGC 3 cut(s) 222, 342, 381
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 141, 199
ClaI ATCGAT 1 cut(s) 25
Csp6I GTAC 1 cut(s) 16
CviAII CATG 2 cut(s) 229, 337
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 2 cut(s) 345, 408
DpnI GATC 3 cut(s) 24, 28, 324
DpnII GATC 3 cut(s) 22, 26, 322
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 228
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 141, 199
Eco57I CTGAAG 1 cut(s) 410
EcoO109I RGGNCCY 2 cut(s) 141, 199
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 2 cut(s) 232, 340
FaiI YATR 3 cut(s) 134, 230, 338
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 2 cut(s) 127, 185
FatI CATG 2 cut(s) 228, 336
Fnu4HI GCNGC 4 cut(s) 225, 237, 306, 405
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 4 cut(s) 225, 237, 306, 405
GlaI GCGC 1 cut(s) 126
GluI GCNGC 4 cut(s) 225, 237, 306, 405
GsaI CCCAGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 236
HhaI GCGC 1 cut(s) 127
Hin1II CATG 2 cut(s) 232, 340
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HphI GGTGA 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 2 cut(s) 31, 294
Hpy188III TCNNGA 4 cut(s) 10, 157, 193, 370
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 3 cut(s) 23, 79, 88
HpyAV CCTTC 4 cut(s) 26, 102, 205, 294
HpyCH4III ACNGT 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 2 cut(s) 345, 408
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 2 cut(s) 232, 340
HspAI GCGC 1 cut(s) 125
KflI GGGWCCC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 22, 26, 322
LpnPI CCDG 7 cut(s) 90, 101, 112, 141, 326, 345, 355
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 112
MaeII ACGT 1 cut(s) 18
MalI GATC 3 cut(s) 24, 28, 324
MboI GATC 3 cut(s) 22, 26, 322
MboII GAAGA 5 cut(s) 220, 259, 262, 307, 403
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 151
MnlI CCTC 5 cut(s) 6, 51, 132, 189, 226
MseI TTAA 2 cut(s) 312, 351
MspA1I CMGCKG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 233
NcoI CCATGG 1 cut(s) 228
NdeII GATC 3 cut(s) 22, 26, 322
NlaIII CATG 2 cut(s) 232, 340
NlaIV GGNNCC 3 cut(s) 143, 200, 201
PcsI WCGNNNNNNNCGW 3 cut(s) 71, 80, 83
PkrI GCNGC 4 cut(s) 226, 238, 307, 406
Ple19I CGATCG 2 cut(s) 25, 29
PpuMI RGGWCCY 2 cut(s) 141, 199
Psp5II RGGWCCY 2 cut(s) 141, 199
PspFI CCCAGC 1 cut(s) 359
PspN4I GGNNCC 3 cut(s) 143, 200, 201
PspPI GGNCC 2 cut(s) 141, 199
PspPPI RGGWCCY 2 cut(s) 141, 199
PvuI CGATCG 2 cut(s) 25, 29
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SaqAI TTAA 2 cut(s) 312, 351
SatI GCNGC 4 cut(s) 225, 237, 306, 405
Sau3AI GATC 3 cut(s) 22, 26, 322
Sau96I GGNCC 2 cut(s) 141, 199
SduI GDGCHC 1 cut(s) 115
SetI ASST 8 cut(s) 17, 21, 74, 143, 336, 361, 381, 409
SfaNI GCATC 1 cut(s) 112
SinI GGWCC 2 cut(s) 141, 199
Sse9I AATT 1 cut(s) 151
SsiI CCGC 2 cut(s) 237, 305
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 266
TaiI ACGT 1 cut(s) 21
TaqI TCGA 4 cut(s) 21, 25, 86, 186
TasI AATT 1 cut(s) 151
TauI GCSGC 2 cut(s) 239, 308
Tru1I TTAA 2 cut(s) 312, 351
Tru9I TTAA 2 cut(s) 312, 351
TscAI CASTG 1 cut(s) 178
TseI GCWGC 2 cut(s) 224, 404
TspDTI ATGAA 1 cut(s) 164
TspGWI ACGGA 1 cut(s) 68
TspRI CASTG 1 cut(s) 178
VpaK11BI GGWCC 2 cut(s) 141, 199
XapI RAATTY 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.