pycom13g20620

Superkiller viralicidic activity 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Reverse (-)
16791628 .. 16792137
510 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 510 bp
ATGGAAGAATCTCCAACGCCTGCGAAACGAAAAGAACCTGAAGCGTCAGAGATCACAGAAACTCCCACCGATGAATCTCCCCTGAAGCGACGCCATTTGACTCGAACATGTGTGCATGAGGTTGCAGTTCCCAGTGACTACGCTTCGACCAAGGATGAGTCGGTTTATGGAACCCTTTCAAATCCTCTGTATACTGGCGAGGCGGCCAAGACATACCAATTTACCCTTGACCCCTTTCAGCAAATTTCTGTGGCATGCTTAGAACGGAAGGAATCAGTTTTGGTCTCCGCCCATACTTCAGCTGGTAAGACTGCAGTCGCAGAGTATGCGATTGCCATGGCTTTTAGAGACAAACAGAGGGTTATATATACTTCGCCATTGAAGGCTCTGAGCAACCAGAAGTATAGAGAGCTCAGCCAGGAGTTTGAAGATGTTGGATTGATGACTGGAGATGTTACACTCTCACCAAATGCTAGTTGTTTGGTTATGACTACTGAGGAAGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003002 GO:0003006 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0007275 GO:0007389 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009892 GO:0009893 GO:0009908 GO:0009987 GO:0010093 GO:0010467 GO:0010468 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0016071 GO:0016462 GO:0016604 GO:0016607 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0046700 GO:0048367 GO:0048437 GO:0048444 GO:0048449 GO:0048518 GO:0048519 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0055087 GO:0060147 GO:0060149 GO:0060255 GO:0060968 GO:0060969 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0090701 GO:0099402 GO:0140098 GO:1901360 GO:1901361 GO:1901575 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

170

Amino Acids

18.73

Weight (kDa)

4.96

Isoelectric Point (pI)

57.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 78 - 149 5.4e-13 DEAD/DEAH box helicase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 191
AciI CCGC 2 cut(s) 203, 288
AcoI YGGCCR 1 cut(s) 204
AcsI RAATTY 1 cut(s) 243
AcuI CTGAAG 3 cut(s) 60, 104, 282
AcyI GRCGYC 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 107
AgsI TTSAA 3 cut(s) 180, 382, 428
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 2 cut(s) 302, 412
AluI AGCT 2 cut(s) 302, 412
Alw21I GWGCWC 1 cut(s) 414
Alw26I GTCTC 2 cut(s) 289, 342
AoxI GGCC 1 cut(s) 204
ApoI RAATTY 1 cut(s) 243
Asp700I GAANNNNTTC 1 cut(s) 175
AsuHPI GGTGA 1 cut(s) 456
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 1 cut(s) 414
BciT130I CCWGG 1 cut(s) 419
BcoDI GTCTC 2 cut(s) 289, 342
BfaI CTAG 1 cut(s) 474
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 1 cut(s) 204
BlpI GCTNAGC 1 cut(s) 413
BlsI GCNGC 1 cut(s) 205
Bme1390I CCNGG 1 cut(s) 419
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 1 cut(s) 419
BmrI ACTGGG 1 cut(s) 126
BmuI ACTGGG 1 cut(s) 126
BoxI GACNNNNGTC 1 cut(s) 314
BpmI CTGGAG 1 cut(s) 468
Bpu1102I GCTNAGC 1 cut(s) 413
BsaHI GRCGYC 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 2 cut(s) 150, 336
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bse1I ACTGG 3 cut(s) 132, 199, 451
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 2 cut(s) 150, 336
BseGI GGATG 1 cut(s) 160
BseMII CTCAG 3 cut(s) 380, 427, 486
BseNI ACTGG 3 cut(s) 132, 199, 451
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 414
BsmAI GTCTC 2 cut(s) 289, 342
BsnI GGCC 1 cut(s) 206
Bso31I GGTCTC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 414
Bsp143I GATC 1 cut(s) 51
Bsp1720I GCTNAGC 1 cut(s) 413
Bsp19I CCATGG 1 cut(s) 336
BspACI CCGC 2 cut(s) 203, 288
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 3 cut(s) 381, 426, 487
BspLI GGNNCC 1 cut(s) 172
BspMAI CTGCAG 1 cut(s) 316
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 3 cut(s) 132, 199, 451
BssECI CCNNGG 2 cut(s) 150, 336
BssMI GATC 1 cut(s) 51
BssNAI GTATAC 1 cut(s) 192
BssNI GRCGYC 1 cut(s) 91
BssT1I CCWWGG 2 cut(s) 150, 336
Bst1107I GTATAC 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 419
BstACI GRCGYC 1 cut(s) 91
BstAPI GCANNNNNTGC 1 cut(s) 326
BstC8I GCNNGC 2 cut(s) 21, 256
BstDEI CTNAG 4 cut(s) 259, 389, 413, 495
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 1 cut(s) 160
BstKTI GATC 1 cut(s) 54
BstMAI GTCTC 2 cut(s) 289, 342
BstMBI GATC 1 cut(s) 51
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 2 cut(s) 111, 258
BstPAI GACNNNNGTC 1 cut(s) 314
BstSCI CCNGG 1 cut(s) 417
BstSFI CTRYAG 1 cut(s) 312
BstZ17I GTATAC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 206
BtgI CCRYGG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 21, 256
CseI GACGC 2 cut(s) 33, 99
CviAII CATG 4 cut(s) 108, 116, 255, 337
CviJI RGCY 6 cut(s) 206, 302, 341, 386, 412, 417
CviKI_1 RGCY 6 cut(s) 206, 302, 341, 386, 412, 417
DdeI CTNAG 4 cut(s) 259, 389, 413, 495
DpnI GATC 1 cut(s) 53
DpnII GATC 1 cut(s) 51
EaeI YGGCCR 1 cut(s) 204
EciI GGCGGA 1 cut(s) 277
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 2 cut(s) 150, 336
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 412
Eco57I CTGAAG 3 cut(s) 60, 104, 282
EcoICRI GAGCTC 1 cut(s) 412
EcoRII CCWGG 1 cut(s) 417
EcoT14I CCWWGG 2 cut(s) 150, 336
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 2 cut(s) 150, 336
FaeI CATG 4 cut(s) 111, 119, 258, 340
FatI CATG 4 cut(s) 107, 115, 254, 336
FblI GTMKAC 1 cut(s) 191
Fnu4HI GCNGC 1 cut(s) 204
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 414
Fsp4HI GCNGC 1 cut(s) 204
FspBI CTAG 1 cut(s) 474
GluI GCNGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 1 cut(s) 206
HgaI GACGC 2 cut(s) 33, 99
Hin1I GRCGYC 1 cut(s) 91
Hin1II CATG 4 cut(s) 111, 119, 258, 340
HinfI GANTC 5 cut(s) 8, 74, 100, 158, 272
HphI GGTGA 1 cut(s) 456
Hpy166II GTNNAC 1 cut(s) 192
Hpy188I TCNGA 3 cut(s) 49, 390, 509
Hpy8I GTNNAC 1 cut(s) 192
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 2 cut(s) 262, 376
HpyCH4V TGCA 3 cut(s) 115, 125, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 4 cut(s) 259, 389, 413, 495
Hsp92I GRCGYC 1 cut(s) 91
Hsp92II CATG 4 cut(s) 111, 119, 258, 340
Kzo9I GATC 1 cut(s) 51
MaeI CTAG 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 134, 454
MalI GATC 1 cut(s) 53
MboI GATC 1 cut(s) 51
MboII GAAGA 2 cut(s) 17, 440
MhlI GDGCHC 1 cut(s) 414
MluCI AATT 2 cut(s) 218, 243
MlyI GAGTC 2 cut(s) 94, 167
MmeI TCCRAC 2 cut(s) 38, 415
MnlI CCTC 5 cut(s) 112, 193, 195, 351, 490
MroXI GAANNNNTTC 1 cut(s) 175
MspA1I CMGCKG 1 cut(s) 302
MspR9I CCNGG 1 cut(s) 419
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 1 cut(s) 326
NcoI CCATGG 1 cut(s) 336
NdeII GATC 1 cut(s) 51
NlaIII CATG 4 cut(s) 111, 119, 258, 340
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 1 cut(s) 134
NspI RCATGY 2 cut(s) 111, 258
PaeI GCATGC 1 cut(s) 258
PciI ACATGT 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 175
PfeI GAWTC 3 cut(s) 8, 74, 272
PkrI GCNGC 1 cut(s) 205
PleI GAGTC 2 cut(s) 94, 166
PpsI GAGTC 2 cut(s) 94, 166
PscI ACATGT 1 cut(s) 107
PshAI GACNNNNGTC 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 414
Psp6I CCWGG 1 cut(s) 417
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 1 cut(s) 172
PstI CTGCAG 1 cut(s) 316
PvuII CAGCTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 414
SatI GCNGC 1 cut(s) 204
Sau3AI GATC 1 cut(s) 51
SchI GAGTC 2 cut(s) 94, 167
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 1 cut(s) 414
SetI ASST 4 cut(s) 40, 123, 304, 414
SfcI CTRYAG 1 cut(s) 312
SphI GCATGC 1 cut(s) 258
Sse9I AATT 2 cut(s) 218, 243
SsiI CCGC 2 cut(s) 203, 288
SspMI CTAG 1 cut(s) 474
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 150, 336
TaqI TCGA 2 cut(s) 103, 146
TasI AATT 2 cut(s) 218, 243
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 3 cut(s) 8, 74, 272
TscAI CASTG 1 cut(s) 139
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 1 cut(s) 87
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 1 cut(s) 139
XapI RAATTY 1 cut(s) 243
XceI RCATGY 2 cut(s) 111, 258
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 175
XspI CTAG 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.