pycom13g21920

Pre-mRNA-splicing factor ATP-dependent RNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Reverse (-)
18992130 .. 18992568
439 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 390 bp
ATGATAAAACACAATTTTTTTAATATAAAGAGAAGCCAGGCAAAACAAAGAGTTGCAGCCACGTCCGTGGCCAAAAGGGTTGCTGATGAATATGGTTGTCGATTAGGGGAGGAAGTTGGGTACTCTATTCGGTTTGATGATTATACTGGATCGGATACTGCGATCAAGTACATGACCGATCTGATGCTTTTAAGGGAAATTTTGAGTGACAAAAACCTGTCTCAGTACTCCATGATCATGCTTGATGAAGCTCATGAAAAGATGAAAGTAGTTGGTGGAGAAGCTCCGGAACTGATTATTCTACCAGCTTATGGTGCCCAGCCGAGTGCAGAGCAATCAAAGATTTTCAAGCCTGCTTTGCTCCTTCAAATTTGCTTGAAGCCTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind S1

Protein Analysis

130

Amino Acids

14.52

Weight (kDa)

7.74

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 311
AccIII TCCGGA 1 cut(s) 286
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 1 cut(s) 69
AcsI RAATTY 2 cut(s) 198, 369
AfaI GTAC 3 cut(s) 122, 170, 227
AfiI CCNNNNNNNGG 1 cut(s) 311
AgsI TTSAA 3 cut(s) 349, 368, 379
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 1 cut(s) 36
AleI CACNNNNGTG 1 cut(s) 65
AluBI AGCT 3 cut(s) 251, 284, 308
AluI AGCT 3 cut(s) 251, 284, 308
Alw26I GTCTC 1 cut(s) 225
AlwI GGATC 1 cut(s) 157
Aor13HI TCCGGA 1 cut(s) 286
AoxI GGCC 1 cut(s) 69
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 2 cut(s) 198, 369
BaeGI GKGCMC 1 cut(s) 319
BalI TGGCCA 1 cut(s) 71
BanI GGYRCC 1 cut(s) 314
BbvI GCAGC 1 cut(s) 68
BciT130I CCWGG 1 cut(s) 38
BciVI GTATCC 1 cut(s) 148
BclI TGATCA 1 cut(s) 234
BcoDI GTCTC 1 cut(s) 225
BfuI GTATCC 1 cut(s) 148
BisI GCNGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 58
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 38
BmgBI CACGTC 1 cut(s) 63
BmiI GGNNCC 1 cut(s) 316
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 66
BsaWI WCCGGW 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 151
BseAI TCCGGA 1 cut(s) 286
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 66
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 151
BseSI GKGCMC 1 cut(s) 319
BseXI GCAGC 1 cut(s) 68
BseYI CCCAGC 1 cut(s) 318
BsgI GTGCAG 1 cut(s) 348
BshFI GGCC 1 cut(s) 71
BshNI GGYRCC 1 cut(s) 314
BsiSI CCGG 1 cut(s) 287
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 1 cut(s) 225
BsnI GGCC 1 cut(s) 71
Bsp1286I GDGCHC 1 cut(s) 319
Bsp13I TCCGGA 1 cut(s) 286
Bsp143I GATC 4 cut(s) 149, 162, 178, 234
BspANI GGCC 1 cut(s) 71
BspCNI CTCAG 1 cut(s) 235
BspEI TCCGGA 1 cut(s) 286
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 316
BspPI GGATC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 314
BsrI ACTGG 1 cut(s) 151
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 4 cut(s) 149, 162, 178, 234
Bst2UI CCWGG 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 66
BstKTI GATC 4 cut(s) 152, 165, 181, 237
BstMAI GTCTC 1 cut(s) 225
BstMBI GATC 4 cut(s) 149, 162, 178, 234
BstMWI GCNNNNNNNGC 2 cut(s) 314, 358
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 319
BstV1I GCAGC 1 cut(s) 68
BstXI CCANNNNNNTGG 1 cut(s) 67
BsuI GTATCC 1 cut(s) 148
BsuRI GGCC 1 cut(s) 71
BtgI CCRYGG 1 cut(s) 66
BtrI CACGTC 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 354
CciI TCATGA 1 cut(s) 253
Csp6I GTAC 3 cut(s) 121, 169, 226
CviAII CATG 4 cut(s) 172, 232, 238, 254
CviJI RGCY 9 cut(s) 36, 59, 71, 251, 284, 308, 322, 352, 382
CviKI_1 RGCY 9 cut(s) 36, 59, 71, 251, 284, 308, 322, 352, 382
CviQI GTAC 3 cut(s) 121, 169, 226
DdeI CTNAG 1 cut(s) 222
DpnI GATC 4 cut(s) 151, 164, 180, 236
DpnII GATC 4 cut(s) 149, 162, 178, 234
EaeI YGGCCR 1 cut(s) 69
EcoRII CCWGG 1 cut(s) 36
FaeI CATG 4 cut(s) 175, 235, 241, 257
FaiI YATR 8 cut(s) 26, 93, 144, 173, 233, 239, 255, 312
FatI CATG 4 cut(s) 171, 231, 237, 253
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 57
Fsp4HI GCNGC 1 cut(s) 57
GluI GCNGC 1 cut(s) 57
GsaI CCCAGC 1 cut(s) 322
HaeIII GGCC 1 cut(s) 71
HapII CCGG 1 cut(s) 287
Hin1II CATG 4 cut(s) 175, 235, 241, 257
HpaII CCGG 1 cut(s) 287
Hpy188I TCNGA 2 cut(s) 154, 183
Hpy188III TCNNGA 2 cut(s) 254, 287
HpyAV CCTTC 1 cut(s) 374
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 2 cut(s) 56, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 358
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 4 cut(s) 175, 235, 241, 257
Kpn2I TCCGGA 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 4 cut(s) 149, 162, 178, 234
LmnI GCTCC 2 cut(s) 289, 366
LpnPI CCDG 8 cut(s) 23, 50, 132, 230, 300, 318, 332, 366
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 1 cut(s) 174
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 206
MalI GATC 4 cut(s) 151, 164, 180, 236
MboI GATC 4 cut(s) 149, 162, 178, 234
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 71
MluCI AATT 3 cut(s) 13, 198, 369
MluNI TGGCCA 1 cut(s) 71
MnlI CCTC 1 cut(s) 103
Mox20I TGGCCA 1 cut(s) 71
MroI TCCGGA 1 cut(s) 286
MscI TGGCCA 1 cut(s) 71
MseI TTAA 2 cut(s) 21, 191
MslI CAYNNNNRTG 2 cut(s) 65, 236
Msp20I TGGCCA 1 cut(s) 71
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 314, 358
NdeII GATC 4 cut(s) 149, 162, 178, 234
NlaIII CATG 4 cut(s) 175, 235, 241, 257
NlaIV GGNNCC 1 cut(s) 316
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 206
OliI CACNNNNGTG 1 cut(s) 65
PagI TCATGA 1 cut(s) 253
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PflMI CCANNNNNTGG 1 cut(s) 311
PkrI GCNGC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 318
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 1 cut(s) 316
RsaI GTAC 3 cut(s) 122, 170, 227
RsaNI GTAC 3 cut(s) 121, 169, 226
RseI CAYNNNNRTG 2 cut(s) 65, 236
SaqAI TTAA 2 cut(s) 21, 191
SatI GCNGC 1 cut(s) 57
Sau3AI GATC 4 cut(s) 149, 162, 178, 234
ScaI AGTACT 1 cut(s) 227
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 319
SetI ASST 5 cut(s) 65, 219, 253, 286, 310
SfaNI GCATC 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 65, 236
Sse9I AATT 3 cut(s) 13, 198, 369
StyD4I CCNGG 1 cut(s) 36
TaiI ACGT 1 cut(s) 65
TaqI TCGA 1 cut(s) 100
TaqII GACCGA 1 cut(s) 191
TasI AATT 3 cut(s) 13, 198, 369
TatI WGTACW 2 cut(s) 168, 225
Tru1I TTAA 2 cut(s) 21, 191
Tru9I TTAA 2 cut(s) 21, 191
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 4 cut(s) 102, 261, 270, 278
TspGWI ACGGA 1 cut(s) 55
Van91I CCANNNNNTGG 1 cut(s) 311
XapI RAATTY 2 cut(s) 198, 369
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.