pycom13g22890

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
20676350 .. 20678284
1935 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1440 bp
ATGTTGGCCTTTTTGAAACTAGTGCCACCAAGTAATGCAGTCCTCCACGCTGTTTACCCAAACCAATCGGTTACTGAACAAGCCATTGCGCTAGTGACCTTGCTTACAGACATTAAGTCAACTTTGAAAGTTGGCACACACAATACATCTTGTGATAGGAGCCTCCTGTCCACTAGTGAATTTGGTGAAGATGTAATATGGTCTGTCGCACCACTGTCGATTATCCATCGACAAAGATGCTTCAGAGGTTTTAACAAACCTGATGAGTTTTCAGTGGCATTGGCAGCAGCATTGGCTTTGGGAGCATGTTGTGCTATCTCATTGTTAGACCAGGTACAACGGAATGCAGTTCTTGCAGAGAAACCCCATTGGTGGTATTGTTGGCAAAACTTCCATGGCGTTTGTTGCGTCCACCACCGTTGTTCCCACACCCACCACCTTTGTTGCTACGATGAAGATGATGTTCGGGAGGATATCCATGCAGTTTGCAGCATGTGTCAATGGTATGATGGGAGTTTCTGCCAGTTTGCGACTTCTGCCATTGACAAACCACCATTGTTGATACCTTTGCAGCTTCACGTGAATCGCTCACATTGCGCTGTTTTTCTTACTGGACAATCATGGAATACGTTGAATGCACAGAGGGTAATGGACTCATCATCAGAATGTTGCTACGCGCATTCTCTCCGTTTGATCGACCTTGCTTCTTTAAAGCACCGCAGGTGCAAGATGCATGATCATTATGGGATGCAAGTTCATCCCACAATCTCTTCAGTTTGGTATATCTTGTTGAATTTGATAAATTCTGGGTGCATTACCTTGGCTGAAACAATTTTCAAGATCCTGCCAAATTTCTTAGGGGGTGGTGTACCAAGAAACGATCATGTCATTGCACTGTTTCTAGGAAGCAAAGTCATCTGGTTTCTTGATTTCATCATGAGCCTTCAGTGTGCCATCCATAAAACCCAGCTTGTTCTTAGCACTCAGAGAAAGCTTCACAAAACGACACCAAGTGAAATAATTGTCTCTATTGAAAAGCTTGGAGACGAGAACCATCGCCAGATGATCAAAATGGTGAATGAAAAAATAACTGGAAATAAGAAAAATGCAGAAGAAAAAAAATGCAGTGAGAAGAAACTAGGGTCCACTTGGATTTCCCTACTACACAAGTTAGAAGTAAATATAAAAAGGGTAAGGAGAGAAAGCACAACACACAGGGCTTCACCAACTTCCATTAGGTTTCTTATATTAATTATTTCAACAGTAGTGCACTGTGATTTGGAGCCTATCAATATCTTTGTTGATGAAGACATAGTTGCACATGTGGGAGAATTCGGTCTTGCTACAATTTTAGCAGGAAATAAGGATGCAACACAAACCAGAACTCTTGGTATGCTTGGTTATGTTGCACCTGTAATACATATTCTGAAAACACAATAA

Protein Analysis

480

Amino Acids

53.83

Weight (kDa)

8.3

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 711
Acc36I ACCTGC 1 cut(s) 711
AccII CGCG 1 cut(s) 677
AciI CCGC 1 cut(s) 718
AclWI GGATC 1 cut(s) 835
AcsI RAATTY 5 cut(s) 179, 793, 802, 850, 1331
AcuI CTGAAG 3 cut(s) 226, 756, 929
AcvI CACGTG 1 cut(s) 580
AdeI CACNNNGTG 1 cut(s) 1013
AfaI GTAC 2 cut(s) 336, 870
AfiI CCNNNNNNNGG 1 cut(s) 372
AflIII ACRYGT 1 cut(s) 1321
AgsI TTSAA 7 cut(s) 16, 127, 634, 793, 838, 1034, 1260
AhdI GACNNNNNGTC 1 cut(s) 115
AhlI ACTAGT 2 cut(s) 19, 173
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 4 cut(s) 574, 970, 994, 1039
AluI AGCT 4 cut(s) 574, 970, 994, 1039
Alw21I GWGCWC 1 cut(s) 1272
Alw26I GTCTC 2 cut(s) 1030, 1038
Alw44I GTGCAC 1 cut(s) 1268
AlwI GGATC 1 cut(s) 835
AoxI GGCC 1 cut(s) 6
ApaLI GTGCAC 1 cut(s) 1268
ApeKI GCWGC 4 cut(s) 284, 287, 489, 571
ApoI RAATTY 5 cut(s) 179, 793, 802, 850, 1331
AseI ATTAAT 1 cut(s) 1250
AspLEI GCGC 3 cut(s) 91, 599, 679
AspS9I GGNCC 1 cut(s) 1143
AsuHPI GGTGA 3 cut(s) 197, 1087, 1215
AvaII GGWCC 1 cut(s) 1143
BaeGI GKGCMC 1 cut(s) 1272
BbrPI CACGTG 1 cut(s) 580
BbsI GAAGAC 1 cut(s) 1314
Bbv12I GWGCWC 1 cut(s) 1272
BbvI GCAGC 4 cut(s) 296, 299, 501, 583
BccI CCATC 4 cut(s) 234, 503, 962, 1062
BcgI CGANNNNNNTGC 4 cut(s) 198, 219, 232, 253
BciT130I CCWGG 1 cut(s) 332
BclI TGATCA 2 cut(s) 736, 1065
BcoDI GTCTC 2 cut(s) 1030, 1038
BcuI ACTAGT 2 cut(s) 19, 173
BfaI CTAG 5 cut(s) 20, 92, 174, 902, 1139
BfuAI ACCTGC 1 cut(s) 711
BisI GCNGC 4 cut(s) 285, 288, 490, 572
BlsI GCNGC 4 cut(s) 286, 289, 491, 573
Bme1390I CCNGG 1 cut(s) 332
Bme18I GGWCC 1 cut(s) 1143
BmeRI GACNNNNNGTC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 1143
BmiI GGNNCC 3 cut(s) 161, 1144, 1284
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 4 cut(s) 227, 720, 738, 1357
BpiI GAAGAC 1 cut(s) 1314
BsaAI YACGTR 1 cut(s) 580
BsaJI CCNNGG 2 cut(s) 394, 819
Bsc4I CCNNNNNNNGG 1 cut(s) 372
Bse1I ACTGG 3 cut(s) 523, 616, 1096
Bse3DI GCAATG 3 cut(s) 84, 592, 888
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 2 cut(s) 394, 819
BseGI GGATG 4 cut(s) 753, 757, 954, 1372
BseLI CCNNNNNNNGG 1 cut(s) 372
BseMI GCAATG 3 cut(s) 84, 592, 888
BseMII CTCAG 1 cut(s) 998
BseNI ACTGG 3 cut(s) 523, 616, 1096
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 4 cut(s) 296, 299, 501, 583
BseYI CCCAGC 1 cut(s) 966
Bsh1236I CGCG 1 cut(s) 677
BshFI GGCC 1 cut(s) 8
BsiHKAI GWGCWC 1 cut(s) 1272
BslI CCNNNNNNNGG 1 cut(s) 372
BsmAI GTCTC 2 cut(s) 1030, 1038
BsmBI CGTCTC 1 cut(s) 1038
BsmI GAATGC 3 cut(s) 349, 640, 679
BsnI GGCC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 1272
Bsp143I GATC 5 cut(s) 693, 736, 840, 880, 1065
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 1 cut(s) 718
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 1 cut(s) 997
BspFNI CGCG 1 cut(s) 677
BspHI TCATGA 1 cut(s) 936
BspLI GGNNCC 3 cut(s) 161, 1144, 1284
BspMI ACCTGC 1 cut(s) 711
BspPI GGATC 1 cut(s) 835
BsrDI GCAATG 3 cut(s) 84, 592, 888
BsrI ACTGG 3 cut(s) 523, 616, 1096
BssECI CCNNGG 2 cut(s) 394, 819
BssMI GATC 5 cut(s) 693, 736, 840, 880, 1065
BssT1I CCWWGG 2 cut(s) 394, 819
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 5 cut(s) 216, 419, 897, 1264, 1274
Bst6I CTCTTC 1 cut(s) 775
BstAPI GCANNNNNTGC 2 cut(s) 311, 353
BstBAI YACGTR 1 cut(s) 580
BstDEI CTNAG 3 cut(s) 856, 977, 984
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 4 cut(s) 753, 757, 954, 1372
BstFNI CGCG 1 cut(s) 677
BstHHI GCGC 3 cut(s) 91, 599, 679
BstKTI GATC 5 cut(s) 696, 739, 843, 883, 1068
BstMAI GTCTC 2 cut(s) 1030, 1038
BstMBI GATC 5 cut(s) 693, 736, 840, 880, 1065
BstMWI GCNNNNNNNGC 8 cut(s) 284, 293, 302, 311, 353, 405, 536, 594
BstNI CCWGG 1 cut(s) 332
BstNSI RCATGY 3 cut(s) 309, 496, 1325
BstSCI CCNGG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 1 cut(s) 677
BstV1I GCAGC 4 cut(s) 296, 299, 501, 583
BstV2I GAAGAC 1 cut(s) 1314
BstX2I RGATCY 1 cut(s) 840
BstYI RGATCY 1 cut(s) 840
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 394
BtgZI GCGATG 1 cut(s) 1040
BtsCI GGATG 4 cut(s) 753, 757, 954, 1372
BtsI GCAGTG 1 cut(s) 1132
BtsIMutI CAGTG 6 cut(s) 212, 279, 893, 953, 1132, 1270
BveI ACCTGC 1 cut(s) 711
CciI TCATGA 1 cut(s) 936
CfoI GCGC 3 cut(s) 91, 599, 679
Cfr13I GGNCC 1 cut(s) 1143
CseI GACGC 1 cut(s) 397
CsiI ACCWGGT 1 cut(s) 330
Csp6I GTAC 2 cut(s) 335, 869
CspCI CAANNNNNGTGG 8 cut(s) 401, 422, 425, 436, 457, 460, 540, 575
CviAII CATG 9 cut(s) 306, 395, 479, 493, 621, 734, 884, 937, 1322
CviQI GTAC 2 cut(s) 335, 869
DdeI CTNAG 3 cut(s) 856, 977, 984
DpnI GATC 5 cut(s) 695, 738, 842, 882, 1067
DpnII GATC 5 cut(s) 693, 736, 840, 880, 1065
DraI TTTAAA 1 cut(s) 711
DraIII CACNNNGTG 1 cut(s) 1013
DriI GACNNNNNGTC 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 775
Eam1105I GACNNNNNGTC 1 cut(s) 115
EarI CTCTTC 1 cut(s) 775
Eco130I CCWWGG 2 cut(s) 394, 819
Eco32I GATATC 1 cut(s) 475
Eco47I GGWCC 1 cut(s) 1143
Eco57I CTGAAG 3 cut(s) 226, 756, 929
Eco72I CACGTG 1 cut(s) 580
EcoRI GAATTC 1 cut(s) 1331
EcoRII CCWGG 1 cut(s) 330
EcoRV GATATC 1 cut(s) 475
EcoT14I CCWWGG 2 cut(s) 394, 819
EcoT22I ATGCAT 1 cut(s) 735
ErhI CCWWGG 2 cut(s) 394, 819
Esp3I CGTCTC 1 cut(s) 1038
FaeI CATG 9 cut(s) 309, 398, 482, 496, 624, 737, 887, 940, 1325
FatI CATG 9 cut(s) 305, 394, 478, 492, 620, 733, 883, 936, 1321
FbaI TGATCA 2 cut(s) 736, 1065
Fnu4HI GCNGC 4 cut(s) 285, 288, 490, 572
FokI GGATG 4 cut(s) 744, 760, 941, 1379
Fsp4HI GCNGC 4 cut(s) 285, 288, 490, 572
FspBI CTAG 5 cut(s) 20, 92, 174, 902, 1139
GlaI GCGC 3 cut(s) 90, 598, 678
GluI GCNGC 4 cut(s) 285, 288, 490, 572
GsaI CCCAGC 1 cut(s) 970
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 1 cut(s) 397
HhaI GCGC 3 cut(s) 91, 599, 679
Hin1II CATG 9 cut(s) 309, 398, 482, 496, 624, 737, 887, 940, 1325
Hin6I GCGC 3 cut(s) 89, 597, 677
HinP1I GCGC 3 cut(s) 89, 597, 677
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HindIII AAGCTT 2 cut(s) 992, 1037
HinfI GANTC 2 cut(s) 583, 653
HphI GGTGA 3 cut(s) 197, 1087, 1215
Hpy166II GTNNAC 7 cut(s) 55, 120, 171, 412, 869, 1146, 1270
Hpy188I TCNGA 4 cut(s) 245, 664, 987, 1428
Hpy188III TCNNGA 4 cut(s) 467, 838, 926, 937
Hpy8I GTNNAC 7 cut(s) 55, 120, 171, 412, 869, 1146, 1270
HpyAV CCTTC 1 cut(s) 953
HpyCH4III ACNGT 5 cut(s) 216, 419, 897, 1264, 1274
HpyCH4IV ACGT 2 cut(s) 579, 629
HpyF10VI GCNNNNNNNGC 8 cut(s) 284, 293, 302, 311, 353, 405, 536, 594
HpyF3I CTNAG 3 cut(s) 856, 977, 984
HpySE526I ACGT 2 cut(s) 579, 629
Hsp92II CATG 9 cut(s) 309, 398, 482, 496, 624, 737, 887, 940, 1325
HspAI GCGC 3 cut(s) 89, 597, 677
Ksp22I TGATCA 2 cut(s) 736, 1065
Kzo9I GATC 5 cut(s) 693, 736, 840, 880, 1065
LmnI GCTCC 3 cut(s) 159, 302, 1282
Lsp1109I GCAGC 4 cut(s) 296, 299, 501, 583
LweI GCATC 4 cut(s) 227, 720, 738, 1357
MabI ACCWGGT 1 cut(s) 330
MaeI CTAG 5 cut(s) 20, 92, 174, 902, 1139
MaeII ACGT 2 cut(s) 579, 629
MaeIII GTNAC 2 cut(s) 70, 94
MalI GATC 5 cut(s) 695, 738, 842, 882, 1067
MboI GATC 5 cut(s) 693, 736, 840, 880, 1065
MboII GAAGA 6 cut(s) 200, 467, 762, 1124, 1144, 1319
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 1 cut(s) 1272
MluCI AATT 9 cut(s) 179, 793, 802, 831, 850, 1020, 1251, 1331, 1347
MlyI GAGTC 1 cut(s) 647
MnlI CCTC 5 cut(s) 53, 173, 239, 463, 636
Mph1103I ATGCAT 1 cut(s) 735
MseI TTAA 4 cut(s) 114, 252, 710, 1250
MslI CAYNNNNRTG 1 cut(s) 664
MspR9I CCNGG 1 cut(s) 332
Mva1269I GAATGC 3 cut(s) 349, 640, 679
MvaI CCWGG 1 cut(s) 332
MvnI CGCG 1 cut(s) 677
MwoI GCNNNNNNNGC 8 cut(s) 284, 293, 302, 311, 353, 405, 536, 594
NcoI CCATGG 1 cut(s) 394
NdeII GATC 5 cut(s) 693, 736, 840, 880, 1065
NlaIII CATG 9 cut(s) 309, 398, 482, 496, 624, 737, 887, 940, 1325
NlaIV GGNNCC 3 cut(s) 161, 1144, 1284
NmuCI GTSAC 1 cut(s) 94
NsiI ATGCAT 1 cut(s) 735
NspI RCATGY 3 cut(s) 309, 496, 1325
PagI TCATGA 1 cut(s) 936
PaqCI CACCTGC 1 cut(s) 711
PciI ACATGT 1 cut(s) 1321
PctI GAATGC 3 cut(s) 349, 640, 679
PfeI GAWTC 1 cut(s) 583
PkrI GCNGC 4 cut(s) 286, 289, 491, 573
PleI GAGTC 1 cut(s) 647
PmaCI CACGTG 1 cut(s) 580
PmlI CACGTG 1 cut(s) 580
PpsI GAGTC 1 cut(s) 647
Ppu21I YACGTR 1 cut(s) 580
PscI ACATGT 1 cut(s) 1321
PshBI ATTAAT 1 cut(s) 1250
Psp6I CCWGG 1 cut(s) 330
PspCI CACGTG 1 cut(s) 580
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 161, 1144, 1284
PspPI GGNCC 1 cut(s) 1143
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 2 cut(s) 336, 870
RsaNI GTAC 2 cut(s) 335, 869
RseI CAYNNNNRTG 1 cut(s) 664
SaqAI TTAA 4 cut(s) 114, 252, 710, 1250
SatI GCNGC 4 cut(s) 285, 288, 490, 572
Sau3AI GATC 5 cut(s) 693, 736, 840, 880, 1065
Sau96I GGNCC 1 cut(s) 1143
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 1 cut(s) 1272
SexAI ACCWGGT 1 cut(s) 330
SfaNI GCATC 4 cut(s) 227, 720, 738, 1357
SinI GGWCC 1 cut(s) 1143
SmiMI CAYNNNNRTG 1 cut(s) 664
SpeI ACTAGT 2 cut(s) 19, 173
Sse9I AATT 9 cut(s) 179, 793, 802, 831, 850, 1020, 1251, 1331, 1347
SsiI CCGC 1 cut(s) 718
SspMI CTAG 5 cut(s) 20, 92, 174, 902, 1139
StyD4I CCNGG 1 cut(s) 330
StyI CCWWGG 2 cut(s) 394, 819
TaaI ACNGT 5 cut(s) 216, 419, 897, 1264, 1274
TaiI ACGT 2 cut(s) 582, 632
TaqI TCGA 3 cut(s) 218, 229, 696
TaqII GACCGA 1 cut(s) 1325
TasI AATT 9 cut(s) 179, 793, 802, 831, 850, 1020, 1251, 1331, 1347
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 4 cut(s) 114, 252, 710, 1250
Tru9I TTAA 4 cut(s) 114, 252, 710, 1250
TscAI CASTG 6 cut(s) 219, 279, 900, 953, 1132, 1277
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 4 cut(s) 284, 287, 489, 571
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 5 cut(s) 468, 746, 922, 1095, 1320
TspGWI ACGGA 2 cut(s) 355, 677
TspRI CASTG 6 cut(s) 219, 279, 900, 953, 1132, 1277
VneI GTGCAC 1 cut(s) 1268
VpaK11BI GGWCC 1 cut(s) 1143
VspI ATTAAT 1 cut(s) 1250
XapI RAATTY 5 cut(s) 179, 793, 802, 850, 1331
XceI RCATGY 3 cut(s) 309, 496, 1325
XspI CTAG 5 cut(s) 20, 92, 174, 902, 1139
Zsp2I ATGCAT 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.