pycom13g23820

Epidermal growth factor-like domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024690
Physical Location & Seq
Forward (+)
21193277 .. 21197376
4100 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3111 bp
ATGCGACCTGATATCTTTGACTCTTCCTCTCAGGATGCTGTATCGGATTTTCTGGTAACTCGACAGAGGTTACCGTGCCCAGTCTCACTAGCCAAAGCTGACCTAGAATCCCATTATAAGATAGACGGGTCGTTAGCTATTGTGAACGTCTGCTTTAAGACCACTAGGTGTGTTTTTACGTCGAGTATTATTTTGCTGATGGCAGTAAAGAGTAGGTTGACTATGGTGTCCTTGTCCACTATCATGTTGTCAACTATGGTATGGGCTAATTGGTTATATTCTACCAAAGTATGGTCGTGTGGAAAGTCTACACATTCGGCATCCTACTCGGTAAAGCTAACGATGGGTCCAAGGTTGACGTCGGTTGGCTGGACCTGTGAGACCGATATGTGTTCGGATTCTGTGAAGATGTCGTTGATTCCAATTGTCTTGGCAAGTGGCACTTTGTCTGCGTCTGCGTTCCTCCTAGGCTGCGTAGCTGGTTGTCCAGATATCTGTCGCATTCACCCTTCTTCACTAGCTTCTCTAGGTAATCCTTCCATGTATAGCATATTTAATGTGATCCAACTTGGAGGTTCGCTCTTCGTCTATCTAGGCAGCTTGAACCAAGGTTCGCTTTTGAGTTCTTGGTGGTCGGGGACCGATCTTTACACTGGGTCCTTTGGTTGCTCTTATAGTTTGACTGCTTCTTGTCCACTTGTTGAGTACATAAGGTTTTACTTTTACGCTCTCTTTTGCTTAGGTTTTTTACTTAAAATCTCTATGCATCACAATGGCTTGAAGAAATCATCTTATTTTGAATATTTTAAGAGCCAAGAATGGATAAAGTGGCCTAAGATCCACCAAAAAAGGCCCTTGCAGAACAACCAACTTGCGAGCTTTGCCCCGAAGTGCTCAGAACGAATTAGAAAATGGGCCATATATGGTTTGAAAACCCTGGAAGTCTTTTTTCTAATGGTCTTTATAACTTGTGATTTGGAGGTTTCGACAAGAAATTATGACAGAATAAAGAGGAGCTGTTCAGTTACAAATTCTAATAACACTATAAATGAGAGTCAATTTAAGGATGTAAATGTGTCCGATGTTAGAAAATTCCTAGGCCTTTTAATAGGAGAAAAATACACAGAATCTGCAGGAGAGGAAAATGAGGAGATACACAACATCATAGAGGATGAGAAGTCCCATACTTTGATTAGATTATGGATACTTAGGGCTTTTCAGTTTATCAATTTCATTTGCAAGCTAACATCAGTTTTTTGTCTTAATTGTTTCAAGCACATATTTTCAAGCTTAGCTACCTTGTTTATGTTTCAACATGTGGTTAGGTTCAGAACTAATCATGCCTCTGCTCAGCTTAGGACTGTTGTTGCATCGGCAGCGCCTTGGACTACCACTGTTGGTTTAGGTCGTAGACTGCTAGGTTCGTGTTGTTCCACCATGGGTCTGCTGCTCTGCAGCTTCTTCATCGTGGGTAGGGGATGCTATATGGACCACTGCAACGGCAATTACCGCTGCTGGCATGGCCACGGTGATCAGGATCACCATTGTCTGGCCCTTATCCTTGAACGTAGGAAGTTCTGTTCGTTGTGGTTTCTGAATTTTTCGTCATCATATTTCTTCCCTAGGGAAATTCTAAGAATTTATAATAAAATTCTACGAATGAATGAAAAAAAAGTAGAAGCGTTGAATAACAAAGTCTTCTTCAAGTGTTTGAATCATACTCTCAATGAAAGCACTAATTTGTCAATGCAAATTTTCGCTGTCTTCAGTCTTGACGAAATGCACCTTATCCCATTTAAATTAGGATTGATTACCAAGTTAATTGCAAGATATTATTTCACATACTATCTTTCTCCAAATAGGGCTTGCCACTCCCTTATAAATAGTCCCATTTTTCTCCAAAAATCTAACTTTGGCATTGGAGATTCTTCGGCCAAACCCCCCCCCCCCCCCCCGGCCTATTCATCATGGGCACGTGAGGCTTTTGGCCTTGATCTTAGGTGCAATTATTTTGTAGGTGCATTTTCTTCAAAGGACAAGACGGCGGAAATTTGCATCCACAAATTGGTACTTTTATTAAGAGCCTCAATTCATGCGCTCCAAGAAGACTATCGCATTCAAAGTTTCTCATTTCTTGCTCATTTTACAATGGCTAGAAATTCGGAAACCACAACGAACAGAATTCCTTACGTTCAAGGATTTAGACCACTGCGATCTATGAGGTTCAACGCAACAACAAATGGAGCGGCACCACCCCCACTAAGCACAACTATGCCCGCTGCCCACGGTGAGCAGGCCACCTCGCAGCAGCAGGCCCAAGCCCGTGAAACTAAAGATTATCTAAATTCAAAATTTAAGATGAAGGACTTAAATGAAGTAGATATTATCTTAGGAATTAAAGTACAGAAGCATAGTAGGGGTTACACCTTGTATCAGTCACATTATATAGAAAAATTGCTTCTTAATGAAACTTTGCTTAATAATTCTGGTAGGTCCGTCGCACAACTTGAGTATGCTAGTGTAATTGGAAGTTTAATGTATGCCATGCATTGTACCAGGCCATATATCACATTTGTAGCAAGCAAAATTTCTAGATACACTAGTCATCCAAGTACTAGGACTATTAACCTGAATTTAACTTACTCTGAAACAAGTGCTAATGATAATAAGTCTACATCAGGGTGGATTTTTACAATTGCCGGAAGAGCTTTTTCTTGTGCTTCGAAGAAGCAAACATGTATAGCACATTCAACCATGGAATCTGAATTTATAGCATTAGCGACAATAGGTAAAGAAACAGAATGGATTAGAAATTTGTTATTGGAAATAGAGTTATGGCCACAACCAATGTCATCCATTTCCTTATATTGTGGTAGTGAGGCTACTTTGTCTACATCATACAATAAGGTATACAATGGAAAGTCTAGACATATTAGCTTGAGACATGAATATGTCAAGCAATTAATAACTGATGGAGTTATTAATATTATTTATGTTAAATCAAGTAATAACTTAGCAGATCCATCTTGGTGTAGTAACAAAGCACTTTCAAGAGCTTTGGTTGTTAGTACATCTAGTGGAATGGGGCTAAAACCTTTTACTGAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin Pkinase

Protein Analysis

1037

Amino Acids

116.99

Weight (kDa)

9.18

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 769 - 832 1.4e-06 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024690)

Species Orthologous Gene IDs
pyrus_communis pycom13g23820 pycom13g24530 pycom13g27950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 117, 965, 1644, 1880
AasI GACNNNNNNGTC 1 cut(s) 226
AatII GACGTC 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 2248
AccB7I CCANNNNNTGG 3 cut(s) 291, 1549, 2065
AccBSI CCGCTC 1 cut(s) 2246
AccI GTMKAC 5 cut(s) 308, 1411, 2672, 2891, 2910
AciI CCGC 4 cut(s) 1510, 2045, 2246, 2277
AclWI GGATC 4 cut(s) 556, 832, 1545, 3014
AcoI YGGCCR 3 cut(s) 1522, 1932, 2837
AcuI CTGAAG 1 cut(s) 1750
AcvI CACGTG 1 cut(s) 1976
AcyI GRCGYC 1 cut(s) 359
AdeI CACNNNGTG 1 cut(s) 168
AfaI GTAC 6 cut(s) 707, 2070, 2403, 2554, 2614, 3070
AfiI CCNNNNNNNGG 4 cut(s) 291, 1549, 1954, 2065
AflIII ACRYGT 2 cut(s) 1315, 2735
AhlI ACTAGT 1 cut(s) 2600
AjnI CCWGG 2 cut(s) 936, 2555
AloI GAACNNNNNNTCC 2 cut(s) 1564, 1596
Alw21I GWGCWC 1 cut(s) 896
Alw26I GTCTC 3 cut(s) 88, 374, 2935
AlwI GGATC 4 cut(s) 556, 832, 1545, 3014
AlwNI CAGNNNCTG 1 cut(s) 1130
ApeKI GCWGC 9 cut(s) 471, 597, 1376, 1447, 1455, 1512, 2279, 2305, 2308
AseI ATTAAT 2 cut(s) 2963, 2982
AspA2I CCTAGG 3 cut(s) 466, 1096, 1622
AspLEI GCGC 2 cut(s) 1381, 2098
AsuC2I CCSGG 1 cut(s) 1955
AsuHPI GGTGA 4 cut(s) 497, 1532, 1541, 2300
AsuII TTCGAA 1 cut(s) 2723
AvaII GGWCC 6 cut(s) 347, 372, 639, 657, 1489, 2493
AvrII CCTAGG 3 cut(s) 466, 1096, 1622
BaeGI GKGCMC 2 cut(s) 80, 1975
BaeI ACNNNNGTAYC 2 cut(s) 2587, 2620
BalI TGGCCA 2 cut(s) 1524, 2839
BanI GGYRCC 1 cut(s) 2248
BbrPI CACGTG 1 cut(s) 1976
BbsI GAAGAC 3 cut(s) 1690, 1756, 2112
Bbv12I GWGCWC 1 cut(s) 896
BbvI GCAGC 9 cut(s) 458, 609, 1388, 1434, 1467, 1499, 2266, 2317, 2320
BccI CCATC 4 cut(s) 193, 337, 2966, 3031
BceAI ACGGC 2 cut(s) 1516, 2058
BcgI CGANNNNNNTGC 2 cut(s) 309, 343
BciT130I CCWGG 2 cut(s) 938, 2557
BciVI GTATCC 1 cut(s) 1197
BclI TGATCA 1 cut(s) 1531
BcnI CCSGG 1 cut(s) 1955
BcoDI GTCTC 3 cut(s) 88, 374, 2935
BcuI ACTAGT 1 cut(s) 2600
BfmI CTRYAG 2 cut(s) 1131, 1453
BfoI RGCGCY 1 cut(s) 1382
BfuI GTATCC 1 cut(s) 1197
BlnI CCTAGG 3 cut(s) 466, 1096, 1622
BlpI GCTNAGC 2 cut(s) 1291, 1350
BmcAI AGTACT 1 cut(s) 2614
Bme1390I CCNGG 3 cut(s) 938, 1955, 2557
Bme18I GGWCC 6 cut(s) 347, 372, 639, 657, 1489, 2493
BmiI GGNNCC 4 cut(s) 348, 640, 658, 2250
BmrFI CCNGG 3 cut(s) 938, 1955, 2557
BmrI ACTGGG 2 cut(s) 74, 663
BmsI GCATC 6 cut(s) 25, 329, 775, 1379, 1469, 2064
BmuI ACTGGG 2 cut(s) 74, 663
BpiI GAAGAC 3 cut(s) 1690, 1756, 2112
BplI GAGNNNNNCTC 2 cut(s) 564, 596
Bpu10I CCTNAGC 2 cut(s) 739, 1355
Bpu1102I GCTNAGC 2 cut(s) 1291, 1350
Bpu14I TTCGAA 1 cut(s) 2723
BpuEI CTTGAG 2 cut(s) 2528, 2959
BpuMI CCSGG 1 cut(s) 1955
BsaAI YACGTR 1 cut(s) 1976
BsaBI GATNNNNATC 1 cut(s) 1536
BsaHI GRCGYC 1 cut(s) 359
BsaI GGTCTC 1 cut(s) 374
Bsc4I CCNNNNNNNGG 4 cut(s) 291, 1549, 1954, 2065
Bse1I ACTGG 2 cut(s) 80, 658
Bse8I GATNNNNATC 1 cut(s) 1536
BseBI CCWGG 2 cut(s) 938, 2557
BseGI GGATG 8 cut(s) 40, 320, 1072, 1177, 1484, 2055, 2605, 2852
BseJI GATNNNNATC 1 cut(s) 1536
BseLI CCNNNNNNNGG 4 cut(s) 291, 1549, 1954, 2065
BseMII CTCAG 3 cut(s) 44, 909, 1364
BseNI ACTGG 2 cut(s) 80, 658
BseRI GAGGAG 2 cut(s) 1027, 1163
BseSI GKGCMC 2 cut(s) 80, 1975
BseXI GCAGC 9 cut(s) 458, 609, 1388, 1434, 1467, 1499, 2266, 2317, 2320
BshNI GGYRCC 1 cut(s) 2248
BsiHKAI GWGCWC 1 cut(s) 896
BsiSI CCGG 2 cut(s) 1955, 2700
BslFI GGGAC 3 cut(s) 652, 1165, 1872
BslI CCNNNNNNNGG 4 cut(s) 291, 1549, 1954, 2065
BsmAI GTCTC 3 cut(s) 88, 374, 2935
BsmFI GGGAC 3 cut(s) 652, 1165, 1872
BsmI GAATGC 2 cut(s) 501, 2115
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 2723
Bsp1286I GDGCHC 3 cut(s) 80, 896, 1975
Bsp143I GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
Bsp1720I GCTNAGC 2 cut(s) 1291, 1350
Bsp19I CCATGG 2 cut(s) 1437, 2754
BspACI CCGC 4 cut(s) 1510, 2045, 2246, 2277
BspCNI CTCAG 3 cut(s) 43, 908, 1363
BspLI GGNNCC 4 cut(s) 348, 640, 658, 2250
BspMAI CTGCAG 2 cut(s) 1135, 1457
BspPI GGATC 4 cut(s) 556, 832, 1545, 3014
BspQI GCTCTTC 2 cut(s) 587, 2698
BspT104I TTCGAA 1 cut(s) 2723
BspT107I GGYRCC 1 cut(s) 2248
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 2246
BsrI ACTGG 2 cut(s) 80, 658
BssMI GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
BssNAI GTATAC 1 cut(s) 2911
BssNI GRCGYC 1 cut(s) 359
BssT1I CCWWGG 8 cut(s) 350, 466, 607, 1096, 1382, 1437, 1622, 2754
Bst1107I GTATAC 1 cut(s) 2911
Bst2UI CCWGG 2 cut(s) 938, 2557
Bst4CI ACNGT 5 cut(s) 75, 1363, 1396, 1529, 2288
Bst6I CTCTTC 3 cut(s) 28, 587, 2698
BstACI GRCGYC 1 cut(s) 359
BstBAI YACGTR 1 cut(s) 1976
BstBI TTCGAA 1 cut(s) 2723
BstC8I GCNNGC 8 cut(s) 877, 1241, 1517, 1867, 2277, 2295, 2313, 2581
BstDSI CCRYGG 4 cut(s) 1437, 1525, 2284, 2754
BstEII GGTNACC 1 cut(s) 69
BstF5I GGATG 8 cut(s) 40, 320, 1072, 1177, 1484, 2055, 2605, 2852
BstH2I RGCGCY 1 cut(s) 1382
BstHHI GCGC 2 cut(s) 1381, 2098
BstKTI GATC 8 cut(s) 564, 646, 840, 1534, 1540, 1996, 2216, 3022
BstMAI GTCTC 3 cut(s) 88, 374, 2935
BstMBI GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
BstMWI GCNNNNNNNGC 6 cut(s) 881, 1376, 1509, 1521, 1979, 2705
BstNI CCWGG 2 cut(s) 938, 2557
BstNSI RCATGY 2 cut(s) 1319, 2739
BstPI GGTNACC 1 cut(s) 69
BstSCI CCNGG 3 cut(s) 936, 1953, 2555
BstSFI CTRYAG 2 cut(s) 1131, 1453
BstSLI GKGCMC 2 cut(s) 80, 1975
BstV1I GCAGC 9 cut(s) 458, 609, 1388, 1434, 1467, 1499, 2266, 2317, 2320
BstV2I GAAGAC 3 cut(s) 1690, 1756, 2112
BstX2I RGATCY 2 cut(s) 837, 3019
BstYI RGATCY 2 cut(s) 837, 3019
BstZ17I GTATAC 1 cut(s) 2911
BsuI GTATCC 1 cut(s) 1197
BtgI CCRYGG 4 cut(s) 1437, 1525, 2284, 2754
BtsCI GGATG 8 cut(s) 40, 320, 1072, 1177, 1484, 2055, 2605, 2852
BtsI GCAGTG 2 cut(s) 1492, 2207
BtsIMutI CAGTG 4 cut(s) 651, 1392, 1492, 2207
Cac8I GCNNGC 8 cut(s) 877, 1241, 1517, 1867, 2277, 2295, 2313, 2581
CaiI CAGNNNCTG 1 cut(s) 1130
CfoI GCGC 2 cut(s) 1381, 2098
CseI GACGC 1 cut(s) 441
Csp6I GTAC 6 cut(s) 706, 2069, 2402, 2553, 2613, 3069
CviQI GTAC 6 cut(s) 706, 2069, 2402, 2553, 2613, 3069
DpnI GATC 8 cut(s) 563, 645, 839, 1533, 1539, 1995, 2215, 3021
DpnII GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
DraI TTTAAA 1 cut(s) 1798
DraIII CACNNNGTG 1 cut(s) 168
DrdI GACNNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 3 cut(s) 1522, 1932, 2837
Eam1104I CTCTTC 3 cut(s) 28, 587, 2698
EarI CTCTTC 3 cut(s) 28, 587, 2698
EciI GGCGGA 1 cut(s) 2060
Eco130I CCWWGG 8 cut(s) 350, 466, 607, 1096, 1382, 1437, 1622, 2754
Eco147I AGGCCT 1 cut(s) 1101
Eco31I GGTCTC 1 cut(s) 374
Eco32I GATATC 2 cut(s) 13, 493
Eco47I GGWCC 6 cut(s) 347, 372, 639, 657, 1489, 2493
Eco57I CTGAAG 1 cut(s) 1750
Eco72I CACGTG 1 cut(s) 1976
Eco91I GGTNACC 1 cut(s) 69
EcoO109I RGGNCCY 2 cut(s) 657, 852
EcoO65I GGTNACC 1 cut(s) 69
EcoRI GAATTC 1 cut(s) 2181
EcoRII CCWGG 2 cut(s) 936, 2555
EcoRV GATATC 2 cut(s) 13, 493
EcoT14I CCWWGG 8 cut(s) 350, 466, 607, 1096, 1382, 1437, 1622, 2754
EcoT22I ATGCAT 2 cut(s) 768, 2550
ErhI CCWWGG 8 cut(s) 350, 466, 607, 1096, 1382, 1437, 1622, 2754
FalI AAGNNNNNCTT 4 cut(s) 427, 459, 600, 632
FaqI GGGAC 3 cut(s) 652, 1165, 1872
FauI CCCGC 1 cut(s) 2284
FbaI TGATCA 1 cut(s) 1531
FblI GTMKAC 5 cut(s) 308, 1411, 2672, 2891, 2910
FokI GGATG 8 cut(s) 47, 307, 1079, 1184, 1491, 2042, 2592, 2839
GlaI GCGC 2 cut(s) 1380, 2097
HaeII RGCGCY 1 cut(s) 1382
HapII CCGG 2 cut(s) 1955, 2700
HgaI GACGC 1 cut(s) 441
HhaI GCGC 2 cut(s) 1381, 2098
Hin1I GRCGYC 1 cut(s) 359
Hin6I GCGC 2 cut(s) 1379, 2096
HinP1I GCGC 2 cut(s) 1379, 2096
HincII GTYRAC 3 cut(s) 219, 252, 357
HindII GTYRAC 3 cut(s) 219, 252, 357
HindIII AAGCTT 1 cut(s) 1288
HinfI GANTC 9 cut(s) 20, 107, 398, 418, 1054, 1127, 1714, 1925, 2759
HpaII CCGG 2 cut(s) 1955, 2700
HphI GGTGA 4 cut(s) 497, 1532, 1541, 2300
Hpy188I TCNGA 9 cut(s) 46, 397, 898, 1081, 1331, 1596, 2164, 2647, 2764
Hpy188III TCNNGA 7 cut(s) 32, 488, 1535, 1772, 2592, 2925, 3051
Hpy99I CGWCG 3 cut(s) 184, 364, 2501
HpyAV CCTTC 3 cut(s) 519, 546, 2356
HpyCH4III ACNGT 5 cut(s) 75, 1363, 1396, 1529, 2288
HpyCH4IV ACGT 6 cut(s) 147, 179, 359, 1567, 1975, 2190
HpyF10VI GCNNNNNNNGC 6 cut(s) 881, 1376, 1509, 1521, 1979, 2705
HpySE526I ACGT 6 cut(s) 147, 179, 359, 1567, 1975, 2190
Hsp92I GRCGYC 1 cut(s) 359
HspAI GCGC 2 cut(s) 1379, 2096
Ksp22I TGATCA 1 cut(s) 1531
Kzo9I GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
LguI GCTCTTC 2 cut(s) 587, 2698
LmnI GCTCC 3 cut(s) 1014, 2103, 2243
Lsp1109I GCAGC 9 cut(s) 458, 609, 1388, 1434, 1467, 1499, 2266, 2317, 2320
LweI GCATC 6 cut(s) 25, 329, 775, 1379, 1469, 2064
MaeII ACGT 6 cut(s) 147, 179, 359, 1567, 1975, 2190
MaeIII GTNAC 6 cut(s) 55, 69, 1024, 2420, 2436, 3035
MalI GATC 8 cut(s) 563, 645, 839, 1533, 1539, 1995, 2215, 3021
MbiI CCGCTC 1 cut(s) 2246
MboI GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
MfeI CAATTG 2 cut(s) 423, 2694
MflI RGATCY 2 cut(s) 837, 3019
MhlI GDGCHC 3 cut(s) 80, 896, 1975
MlsI TGGCCA 2 cut(s) 1524, 2839
MluNI TGGCCA 2 cut(s) 1524, 2839
MlyI GAGTC 2 cut(s) 14, 1063
MmeI TCCRAC 1 cut(s) 589
Mox20I TGGCCA 2 cut(s) 1524, 2839
Mph1103I ATGCAT 2 cut(s) 768, 2550
MscI TGGCCA 2 cut(s) 1524, 2839
MslI CAYNNNNRTG 6 cut(s) 242, 771, 2270, 2680, 2949, 3028
Msp20I TGGCCA 2 cut(s) 1524, 2839
MspA1I CMGCKG 2 cut(s) 1512, 2279
MspI CCGG 2 cut(s) 1955, 2700
MspR9I CCNGG 3 cut(s) 938, 1955, 2557
MunI CAATTG 2 cut(s) 423, 2694
Mva1269I GAATGC 2 cut(s) 501, 2115
MvaI CCWGG 2 cut(s) 938, 2557
MwoI GCNNNNNNNGC 6 cut(s) 881, 1376, 1509, 1521, 1979, 2705
NciI CCSGG 1 cut(s) 1955
NcoI CCATGG 2 cut(s) 1437, 2754
NdeII GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
NlaIV GGNNCC 4 cut(s) 348, 640, 658, 2250
NmuCI GTSAC 1 cut(s) 2436
NsiI ATGCAT 2 cut(s) 768, 2550
NspI RCATGY 2 cut(s) 1319, 2739
NspV TTCGAA 1 cut(s) 2723
PceI AGGCCT 1 cut(s) 1101
PciI ACATGT 2 cut(s) 1315, 2735
PciSI GCTCTTC 2 cut(s) 587, 2698
PctI GAATGC 2 cut(s) 501, 2115
PfeI GAWTC 7 cut(s) 107, 398, 418, 1127, 1714, 1925, 2759
PflMI CCANNNNNTGG 3 cut(s) 291, 1549, 2065
PleI GAGTC 2 cut(s) 14, 1062
PmaCI CACGTG 1 cut(s) 1976
PmlI CACGTG 1 cut(s) 1976
PpsI GAGTC 2 cut(s) 14, 1062
Ppu21I YACGTR 1 cut(s) 1976
PpuMI RGGWCCY 1 cut(s) 657
PscI ACATGT 2 cut(s) 1315, 2735
PshBI ATTAAT 2 cut(s) 2963, 2982
PsiI TTATAA 4 cut(s) 117, 965, 1644, 1880
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 2 cut(s) 936, 2555
PspCI CACGTG 1 cut(s) 1976
PspEI GGTNACC 1 cut(s) 69
PspGI CCWGG 2 cut(s) 936, 2555
PspN4I GGNNCC 4 cut(s) 348, 640, 658, 2250
PspPPI RGGWCCY 1 cut(s) 657
PstI CTGCAG 2 cut(s) 1135, 1457
PstNI CAGNNNCTG 1 cut(s) 1130
PsuI RGATCY 2 cut(s) 837, 3019
RsaI GTAC 6 cut(s) 707, 2070, 2403, 2554, 2614, 3070
RsaNI GTAC 6 cut(s) 706, 2069, 2402, 2553, 2613, 3069
RseI CAYNNNNRTG 6 cut(s) 242, 771, 2270, 2680, 2949, 3028
SapI GCTCTTC 2 cut(s) 587, 2698
Sau3AI GATC 8 cut(s) 561, 643, 837, 1531, 1537, 1993, 2213, 3019
ScaI AGTACT 1 cut(s) 2614
SchI GAGTC 2 cut(s) 14, 1063
ScrFI CCNGG 3 cut(s) 938, 1955, 2557
SduI GDGCHC 3 cut(s) 80, 896, 1975
SfaNI GCATC 6 cut(s) 25, 329, 775, 1379, 1469, 2064
SfcI CTRYAG 2 cut(s) 1131, 1453
SfuI TTCGAA 1 cut(s) 2723
SinI GGWCC 6 cut(s) 347, 372, 639, 657, 1489, 2493
SmiI ATTTAAAT 1 cut(s) 1798
SmiMI CAYNNNNRTG 6 cut(s) 242, 771, 2270, 2680, 2949, 3028
SmlI CTYRAG 2 cut(s) 2507, 2938
SmoI CTYRAG 2 cut(s) 2507, 2938
SpeI ACTAGT 1 cut(s) 2600
SseBI AGGCCT 1 cut(s) 1101
SsiI CCGC 4 cut(s) 1510, 2045, 2246, 2277
SspI AATATT 2 cut(s) 803, 2986
StuI AGGCCT 1 cut(s) 1101
StyD4I CCNGG 3 cut(s) 936, 1953, 2555
StyI CCWWGG 8 cut(s) 350, 466, 607, 1096, 1382, 1437, 1622, 2754
SwaI ATTTAAAT 1 cut(s) 1798
TaaI ACNGT 5 cut(s) 75, 1363, 1396, 1529, 2288
TaiI ACGT 6 cut(s) 150, 182, 362, 1570, 1978, 2193
TaqI TCGA 4 cut(s) 61, 182, 986, 2723
TaqII GACCGA 2 cut(s) 398, 656
TatI WGTACW 4 cut(s) 705, 2401, 2612, 3068
TauI GCSGC 1 cut(s) 2249
TfiI GAWTC 7 cut(s) 107, 398, 418, 1127, 1714, 1925, 2759
TscAI CASTG 4 cut(s) 658, 1399, 1499, 2214
TseFI GTSAC 1 cut(s) 2436
TseI GCWGC 9 cut(s) 471, 597, 1376, 1447, 1455, 1512, 2279, 2305, 2308
Tsp45I GTSAC 1 cut(s) 2436
TspGWI ACGGA 1 cut(s) 2485
TspRI CASTG 4 cut(s) 658, 1399, 1499, 2214
Van91I CCANNNNNTGG 3 cut(s) 291, 1549, 2065
VpaK11BI GGWCC 6 cut(s) 347, 372, 639, 657, 1489, 2493
VspI ATTAAT 2 cut(s) 2963, 2982
XbaI TCTAGA 2 cut(s) 2591, 2924
XceI RCATGY 2 cut(s) 1319, 2739
XmaJI CCTAGG 3 cut(s) 466, 1096, 1622
XmiI GTMKAC 5 cut(s) 308, 1411, 2672, 2891, 2910
ZraI GACGTC 1 cut(s) 360
ZrmI AGTACT 1 cut(s) 2614
Zsp2I ATGCAT 2 cut(s) 768, 2550
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.