pycom13g24530

Glutamate-cysteine ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024690
Physical Location & Seq
Forward (+)
21630456 .. 21632267
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1497 bp
ATGTTGATCGATCGATCAATCGAGTCGAGGGAGCGGAAGGATCGATGCTGTGAAGCAGAAATGTATTTTGTACACTGTGAAGACATATATATAGCCTTTGGCTATTTACAAAGATACCCCTTAACTACCTACCTTATAACAGTTCTAAACTATTTTACAGTAACTGAAATGGGTGACTTGTCATTTTCCCTAATATTGAGAGTTGAAATTCACACTCATAGAAGACTCTCTTTTCGTACATTCTTATTATTAGAATTTTTTTATTTGCAAAGATTCTTTGATAAAACGAAAAAGAGAAATACAATGGCTAGAAATTTAGAAAACTCCATGAGTGAAAATTCCAATATTCAAGAAATGGGAACGCGGCGATCCACGAGACTAAATGTGATCCTAGGGGGAGCAACACCACCACCACAAGGTTCCACCATGGGAACCACCGTGGTGGCTACCCGTGGCAAGGTCCATGGCACCACTACCACGGCTCGAGTTGTGCCACCAAAGCAAGCCCAAACCGTGCCATCCAAGGCTCATGGCACCAAGGCCATGACCCAAGCCATGTCATTCCAAGCCCAACGCATTGCCAAGCCGAGCCCTAGCCTTACACCTACATGTGCCATGCGCCGAGCAACCCACTCTCGTGGCCCAACCTGCTCCCACCGAGCAGCCTGCTCTCGTGGCCCAGCCTGTTCCAGTGGTCTAACCTGCTCCCATGGCCCAGCCTACTCTTGCAACCTAGTCAGCTCTCGTGGCCTTCCAAGCAGCCCAAATCGGTCTAAGACTAGTTCAACCATCTCGGCTCCAAATTTCCAGACCGATGATCAAACCGGGAGCATTTTCACCATATTATTCTTCTACCACACTTCCACTGCTCGAGGAGAAACATTCCTTCCAAGCTCTTCCAATCCAAATGGTGAACAACCCTTGTCTCGACAAGTCATAGAGTTGACTAACACCCTAGCACAGCAGACGACCTTGGTGAACCAGCTCTTACAGCGCACGAAGATGCAACGTGTTACAAATGAGGTCAACGTCCCCACAAGCAGCCACTCAACCTGCCATGAACAGAGCGTTTGGGCAGAATACACTCCCGATTGGGCCCCCGAGATAGCGTATACTCTTGTCTTAGCACGTGGAGGAGATTGGGAGCCTTCCAAGCGAAGTGTTTATTCGGAGCTAGCCCCGCAAGTAGCATCCTCCATATCACATCGGGGTAGGCAGCATGACGGGCGGAAAGTAGCAGTTACTCAATCTGGCTCAAGTTCAACTAGCAGCCTGCTAAATCCCTCGCCTGCTAGGAATCCACCACATGCACCGCTGCCGCAGCATAGACGAGCTGAGCACGTAGAAGAGCAGCCTAGACCAGCAGGTCAAGATTGGGGCAGCCGAGAGCTCTACCACTCCCAACAAAGGCAAATTCAAGAAGAAGTAGAAAGGCTTCTGATCGAGCGATTGCGCAATTTCCCGCCCAATGAAGCTATTGACAAGGCGCTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0002213 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004357 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006518 GO:0006575 GO:0006725 GO:0006749 GO:0006750 GO:0006790 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009403 GO:0009404 GO:0009408 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009700 GO:0009719 GO:0009725 GO:0009753 GO:0009791 GO:0009814 GO:0009816 GO:0009908 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010193 GO:0016043 GO:0016143 GO:0016144 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019184 GO:0019438 GO:0019748 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0022414 GO:0032501 GO:0032502 GO:0033036 GO:0033037 GO:0033554 GO:0034641 GO:0042221 GO:0042398 GO:0042430 GO:0042435 GO:0042493 GO:0042545 GO:0042742 GO:0043043 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0044550 GO:0045087 GO:0045229 GO:0046217 GO:0046483 GO:0046677 GO:0046686 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050832 GO:0050896 GO:0051179 GO:0051186 GO:0051188 GO:0051641 GO:0051704 GO:0051707 GO:0051716 GO:0052314 GO:0052315 GO:0052386 GO:0052482 GO:0052542 GO:0052543 GO:0052544 GO:0052545 GO:0061458 GO:0070727 GO:0071554 GO:0071555 GO:0071704 GO:0071840 GO:0090567 GO:0098542 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00270 ko00480 ko01100 map00270 map00480 map01100
Pfam Domains
Protein Families
GCS2

Protein Analysis

499

Amino Acids

55.68

Weight (kDa)

9.55

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024690)

Species Orthologous Gene IDs
pyrus_communis pycom13g23820 pycom13g24530 pycom13g27950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 137
AasI GACNNNNNNGTC 1 cut(s) 1365
Acc16I TGCGCA 1 cut(s) 1454
Acc36I ACCTGC 4 cut(s) 656, 710, 1061, 1355
AccB1I GGYRCC 2 cut(s) 467, 533
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 1112
AccII CGCG 1 cut(s) 364
AciI CCGC 7 cut(s) 34, 364, 1181, 1228, 1313, 1319, 1463
AclWI GGATC 3 cut(s) 48, 363, 382
AcsI RAATTY 6 cut(s) 207, 254, 313, 337, 802, 1413
AcvI CACGTG 1 cut(s) 1130
AfaI GTAC 2 cut(s) 72, 238
AfiI CCNNNNNNNGG 3 cut(s) 416, 457, 1407
AflIII ACRYGT 2 cut(s) 608, 1009
AgsI TTSAA 5 cut(s) 206, 350, 786, 1263, 1418
AhlI ACTAGT 1 cut(s) 779
AleI CACNNNNGTG 2 cut(s) 440, 636
AluBI AGCT 7 cut(s) 741, 894, 985, 1174, 1334, 1390, 1475
AluI AGCT 7 cut(s) 741, 894, 985, 1174, 1334, 1390, 1475
Alw21I GWGCWC 2 cut(s) 1341, 1392
Alw26I GTCTC 2 cut(s) 370, 930
AlwI GGATC 3 cut(s) 48, 363, 382
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 3 cut(s) 483, 870, 1100
AoxI GGCC 6 cut(s) 540, 640, 676, 712, 748, 1095
ApaI GGGCCC 1 cut(s) 1099
ApeKI GCWGC 9 cut(s) 662, 759, 1041, 1216, 1269, 1315, 1321, 1351, 1380
ApoI RAATTY 6 cut(s) 207, 254, 313, 337, 802, 1413
ArsI GACNNNNNNTTYG 2 cut(s) 216, 248
Asp700I GAANNNNTTC 1 cut(s) 1434
AspA2I CCTAGG 1 cut(s) 391
AspLEI GCGC 4 cut(s) 621, 996, 1455, 1489
AspS9I GGNCC 6 cut(s) 460, 641, 677, 713, 1095, 1096
AsuC2I CCSGG 1 cut(s) 826
AsuHPI GGTGA 4 cut(s) 185, 829, 923, 988
AsuNHI GCTAGC 1 cut(s) 1174
AvaI CYCGRG 3 cut(s) 483, 870, 1100
AvaII GGWCC 1 cut(s) 460
AvrII CCTAGG 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 1099
BanI GGYRCC 2 cut(s) 467, 533
BanII GRGCYC 3 cut(s) 593, 1099, 1392
BarI GAAGNNNNNNTAC 2 cut(s) 1419, 1451
BauI CACGAG 4 cut(s) 373, 636, 672, 744
BbrPI CACGTG 1 cut(s) 1130
BbsI GAAGAC 2 cut(s) 87, 229
Bbv12I GWGCWC 2 cut(s) 1341, 1392
BbvI GCAGC 9 cut(s) 674, 771, 1053, 1228, 1281, 1302, 1333, 1363, 1392
BccI CCATC 2 cut(s) 526, 797
BceAI ACGGC 1 cut(s) 495
BcgI CGANNNNNNTGC 2 cut(s) 648, 682
BclI TGATCA 1 cut(s) 817
BcnI CCSGG 1 cut(s) 826
BcoDI GTCTC 2 cut(s) 370, 930
BcuI ACTAGT 1 cut(s) 779
BfoI RGCGCY 1 cut(s) 1490
BfuAI ACCTGC 4 cut(s) 656, 710, 1061, 1355
BlnI CCTAGG 1 cut(s) 391
BlpI GCTNAGC 1 cut(s) 1335
Bme1390I CCNGG 1 cut(s) 826
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 3 cut(s) 483, 870, 1100
BmgT120I GGNCC 6 cut(s) 460, 641, 677, 713, 1095, 1096
BmiI GGNNCC 8 cut(s) 421, 433, 469, 535, 798, 1097, 1098, 1146
BmrFI CCNGG 1 cut(s) 826
BmsI GCATC 3 cut(s) 35, 993, 1199
BmtI GCTAGC 1 cut(s) 1178
BpiI GAAGAC 2 cut(s) 87, 229
Bpu1102I GCTNAGC 1 cut(s) 1335
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 1 cut(s) 826
Bsa29I ATCGAT 3 cut(s) 9, 13, 43
BsaAI YACGTR 2 cut(s) 1130, 1342
Bsc4I CCNNNNNNNGG 3 cut(s) 416, 457, 1407
Bse1I ACTGG 1 cut(s) 690
Bse3DI GCAATG 1 cut(s) 576
BseCI ATCGAT 3 cut(s) 9, 13, 43
BseGI GGATG 2 cut(s) 518, 1190
BseLI CCNNNNNNNGG 3 cut(s) 416, 457, 1407
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 1326
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 888, 1149
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 9 cut(s) 674, 771, 1053, 1228, 1281, 1302, 1333, 1363, 1392
BseYI CCCAGC 2 cut(s) 679, 715
Bsh1236I CGCG 1 cut(s) 364
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 6 cut(s) 542, 642, 678, 714, 750, 1097
BshNI GGYRCC 2 cut(s) 467, 533
BshVI ATCGAT 3 cut(s) 9, 13, 43
BsiEI CGRYCG 1 cut(s) 13
BsiHKAI GWGCWC 2 cut(s) 1341, 1392
BsiHKCI CYCGRG 3 cut(s) 483, 870, 1100
BsiSI CCGG 1 cut(s) 825
BslFI GGGAC 1 cut(s) 1016
BslI CCNNNNNNNGG 3 cut(s) 416, 457, 1407
BsmAI GTCTC 2 cut(s) 370, 930
BsmFI GGGAC 1 cut(s) 1016
BsnI GGCC 6 cut(s) 542, 642, 678, 714, 750, 1097
BsoBI CYCGRG 3 cut(s) 483, 870, 1100
Bsp120I GGGCCC 1 cut(s) 1095
Bsp1286I GDGCHC 4 cut(s) 593, 1099, 1341, 1392
Bsp1407I TGTACA 1 cut(s) 70
Bsp143I GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
Bsp1720I GCTNAGC 1 cut(s) 1335
Bsp19I CCATGG 3 cut(s) 426, 463, 709
BspACI CCGC 7 cut(s) 34, 364, 1181, 1228, 1313, 1319, 1463
BspANI GGCC 6 cut(s) 542, 642, 678, 714, 750, 1097
BspCNI CTCAG 1 cut(s) 1327
BspDI ATCGAT 3 cut(s) 9, 13, 43
BspFNI CGCG 1 cut(s) 364
BspLI GGNNCC 8 cut(s) 421, 433, 469, 535, 798, 1097, 1098, 1146
BspMI ACCTGC 4 cut(s) 656, 710, 1061, 1355
BspOI GCTAGC 1 cut(s) 1178
BspPI GGATC 3 cut(s) 48, 363, 382
BspQI GCTCTTC 2 cut(s) 901, 1341
BspT107I GGYRCC 2 cut(s) 467, 533
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 576
BsrGI TGTACA 1 cut(s) 70
BsrI ACTGG 1 cut(s) 690
BssMI GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
BssNAI GTATAC 1 cut(s) 1113
BssSI CACGAG 4 cut(s) 373, 636, 672, 744
BssT1I CCWWGG 7 cut(s) 391, 426, 463, 522, 537, 709, 972
Bst1107I GTATAC 1 cut(s) 1113
Bst2BI CACGAG 4 cut(s) 373, 636, 672, 744
Bst4CI ACNGT 5 cut(s) 77, 142, 160, 439, 514
Bst6I CTCTTC 2 cut(s) 901, 1341
BstAUI TGTACA 1 cut(s) 70
BstBAI YACGTR 2 cut(s) 1130, 1342
BstC8I GCNNGC 5 cut(s) 504, 667, 1176, 1274, 1290
BstDEI CTNAG 3 cut(s) 774, 1123, 1335
BstDSI CCRYGG 6 cut(s) 426, 438, 451, 463, 477, 709
BstF5I GGATG 2 cut(s) 518, 1190
BstFNI CGCG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 1490
BstHHI GCGC 4 cut(s) 621, 996, 1455, 1489
BstKTI GATC 8 cut(s) 9, 13, 17, 43, 371, 390, 820, 1443
BstMAI GTCTC 2 cut(s) 370, 930
BstMBI GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
BstMCI CGRYCG 1 cut(s) 13
BstNSI RCATGY 2 cut(s) 612, 1310
BstSCI CCNGG 1 cut(s) 824
BstSLI GKGCMC 1 cut(s) 1099
BstUI CGCG 1 cut(s) 364
BstV1I GCAGC 9 cut(s) 674, 771, 1053, 1228, 1281, 1302, 1333, 1363, 1392
BstV2I GAAGAC 2 cut(s) 87, 229
BstXI CCANNNNNNTGG 2 cut(s) 442, 638
BstZ17I GTATAC 1 cut(s) 1113
Bsu15I ATCGAT 3 cut(s) 9, 13, 43
BsuRI GGCC 6 cut(s) 542, 642, 678, 714, 750, 1097
BsuTUI ATCGAT 3 cut(s) 9, 13, 43
BtgI CCRYGG 6 cut(s) 426, 438, 451, 463, 477, 709
BtsCI GGATG 2 cut(s) 518, 1190
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 3 cut(s) 73, 697, 864
BveI ACCTGC 4 cut(s) 656, 710, 1061, 1355
Cac8I GCNNGC 5 cut(s) 504, 667, 1176, 1274, 1290
CaiI CAGNNNCTG 1 cut(s) 164
CfoI GCGC 4 cut(s) 621, 996, 1455, 1489
Cfr13I GGNCC 6 cut(s) 460, 641, 677, 713, 1095, 1096
ClaI ATCGAT 3 cut(s) 9, 13, 43
Csp6I GTAC 2 cut(s) 71, 237
CviQI GTAC 2 cut(s) 71, 237
DdeI CTNAG 3 cut(s) 774, 1123, 1335
DpnI GATC 8 cut(s) 8, 12, 16, 42, 370, 389, 819, 1442
DpnII GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
DrdI GACNNNNNNGTC 1 cut(s) 1365
DseDI GACNNNNNNGTC 1 cut(s) 1365
Eam1104I CTCTTC 2 cut(s) 901, 1341
EarI CTCTTC 2 cut(s) 901, 1341
EciI GGCGGA 1 cut(s) 1243
Ecl136II GAGCTC 1 cut(s) 1390
Eco130I CCWWGG 7 cut(s) 391, 426, 463, 522, 537, 709, 972
Eco24I GRGCYC 3 cut(s) 593, 1099, 1392
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 1390
Eco72I CACGTG 1 cut(s) 1130
Eco88I CYCGRG 3 cut(s) 483, 870, 1100
EcoICRI GAGCTC 1 cut(s) 1390
EcoO109I RGGNCCY 1 cut(s) 1096
EcoT14I CCWWGG 7 cut(s) 391, 426, 463, 522, 537, 709, 972
EcoT38I GRGCYC 3 cut(s) 593, 1099, 1392
ErhI CCWWGG 7 cut(s) 391, 426, 463, 522, 537, 709, 972
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 1016
FauI CCCGC 2 cut(s) 1188, 1470
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 1112
FokI GGATG 2 cut(s) 505, 1177
FriOI GRGCYC 3 cut(s) 593, 1099, 1392
FspI TGCGCA 1 cut(s) 1454
GlaI GCGC 4 cut(s) 620, 995, 1454, 1488
GsaI CCCAGC 2 cut(s) 683, 719
HaeII RGCGCY 1 cut(s) 1490
HaeIII GGCC 6 cut(s) 542, 642, 678, 714, 750, 1097
HapII CCGG 1 cut(s) 825
HhaI GCGC 4 cut(s) 621, 996, 1455, 1489
Hin6I GCGC 4 cut(s) 619, 994, 1453, 1487
HinP1I GCGC 4 cut(s) 619, 994, 1453, 1487
HincII GTYRAC 2 cut(s) 945, 1027
HindII GTYRAC 2 cut(s) 945, 1027
HinfI GANTC 4 cut(s) 23, 225, 273, 1297
HpaII CCGG 1 cut(s) 825
HphI GGTGA 4 cut(s) 185, 829, 923, 988
Hpy166II GTNNAC 6 cut(s) 73, 914, 945, 979, 1027, 1113
Hpy188I TCNGA 2 cut(s) 1171, 1440
Hpy188III TCNNGA 6 cut(s) 350, 808, 927, 1088, 1370, 1418
Hpy8I GTNNAC 6 cut(s) 73, 914, 945, 979, 1027, 1113
HpyAV CCTTC 4 cut(s) 31, 761, 896, 1158
HpyCH4III ACNGT 5 cut(s) 77, 142, 160, 439, 514
HpyCH4IV ACGT 4 cut(s) 1009, 1029, 1129, 1341
HpyCH4V TGCA 4 cut(s) 268, 729, 1006, 1310
HpyF3I CTNAG 3 cut(s) 774, 1123, 1335
HpySE526I ACGT 4 cut(s) 1009, 1029, 1129, 1341
HspAI GCGC 4 cut(s) 619, 994, 1453, 1487
Ksp22I TGATCA 1 cut(s) 817
Kzo9I GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
LguI GCTCTTC 2 cut(s) 901, 1341
LmnI GCTCC 9 cut(s) 31, 398, 656, 710, 802, 828, 1144, 1171, 1494
Lsp1109I GCAGC 9 cut(s) 674, 771, 1053, 1228, 1281, 1302, 1333, 1363, 1392
LweI GCATC 3 cut(s) 35, 993, 1199
MaeII ACGT 4 cut(s) 1009, 1029, 1129, 1341
MaeIII GTNAC 4 cut(s) 160, 173, 1012, 1240
MalI GATC 8 cut(s) 8, 12, 16, 42, 370, 389, 819, 1442
MbiI CCGCTC 1 cut(s) 34
MboI GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
MboII GAAGA 7 cut(s) 92, 234, 841, 888, 1012, 1358, 1433
MhlI GDGCHC 4 cut(s) 593, 1099, 1341, 1392
MluCI AATT 7 cut(s) 207, 254, 313, 337, 802, 1413, 1456
MlyI GAGTC 2 cut(s) 32, 219
MnlI CCTC 6 cut(s) 21, 866, 1015, 1127, 1204, 1294
MroXI GAANNNNTTC 1 cut(s) 1434
MseI TTAA 1 cut(s) 122
MslI CAYNNNNRTG 4 cut(s) 440, 607, 636, 1001
MspA1I CMGCKG 1 cut(s) 1315
MspI CCGG 1 cut(s) 825
MspR9I CCNGG 1 cut(s) 826
MvnI CGCG 1 cut(s) 364
NciI CCSGG 1 cut(s) 826
NcoI CCATGG 3 cut(s) 426, 463, 709
NdeII GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
NheI GCTAGC 1 cut(s) 1174
NlaIV GGNNCC 8 cut(s) 421, 433, 469, 535, 798, 1097, 1098, 1146
NmeAIII GCCGAG 4 cut(s) 612, 647, 773, 1409
NmuCI GTSAC 1 cut(s) 173
NsbI TGCGCA 1 cut(s) 1454
NspI RCATGY 2 cut(s) 612, 1310
OliI CACNNNNGTG 2 cut(s) 440, 636
PaeR7I CTCGAG 2 cut(s) 483, 870
PciI ACATGT 1 cut(s) 608
PciSI GCTCTTC 2 cut(s) 901, 1341
PdmI GAANNNNTTC 1 cut(s) 1434
PfeI GAWTC 2 cut(s) 273, 1297
Ple19I CGATCG 1 cut(s) 13
PleI GAGTC 2 cut(s) 31, 219
PmaCI CACGTG 1 cut(s) 1130
PmlI CACGTG 1 cut(s) 1130
PpsI GAGTC 2 cut(s) 31, 219
Ppu21I YACGTR 2 cut(s) 1130, 1342
PscI ACATGT 1 cut(s) 608
PsiI TTATAA 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 1392
PspCI CACGTG 1 cut(s) 1130
PspFI CCCAGC 2 cut(s) 679, 715
PspN4I GGNNCC 8 cut(s) 421, 433, 469, 535, 798, 1097, 1098, 1146
PspOMI GGGCCC 1 cut(s) 1095
PspPI GGNCC 6 cut(s) 460, 641, 677, 713, 1095, 1096
PspXI VCTCGAGB 2 cut(s) 483, 870
PstNI CAGNNNCTG 1 cut(s) 164
PvuI CGATCG 1 cut(s) 13
RsaI GTAC 2 cut(s) 72, 238
RsaNI GTAC 2 cut(s) 71, 237
RseI CAYNNNNRTG 4 cut(s) 440, 607, 636, 1001
SacI GAGCTC 1 cut(s) 1392
SapI GCTCTTC 2 cut(s) 901, 1341
SaqAI TTAA 1 cut(s) 122
Sau3AI GATC 8 cut(s) 6, 10, 14, 40, 368, 387, 817, 1440
Sau96I GGNCC 6 cut(s) 460, 641, 677, 713, 1095, 1096
SchI GAGTC 2 cut(s) 32, 219
ScrFI CCNGG 1 cut(s) 826
SduI GDGCHC 4 cut(s) 593, 1099, 1341, 1392
SfaNI GCATC 3 cut(s) 35, 993, 1199
Sfr274I CTCGAG 2 cut(s) 483, 870
SinI GGWCC 1 cut(s) 460
SlaI CTCGAG 2 cut(s) 483, 870
SmiMI CAYNNNNRTG 4 cut(s) 440, 607, 636, 1001
SmlI CTYRAG 3 cut(s) 483, 870, 1255
SmoI CTYRAG 3 cut(s) 483, 870, 1255
SpeI ACTAGT 1 cut(s) 779
Sse9I AATT 7 cut(s) 207, 254, 313, 337, 802, 1413, 1456
SsiI CCGC 7 cut(s) 34, 364, 1181, 1228, 1313, 1319, 1463
SspI AATATT 2 cut(s) 195, 346
SstI GAGCTC 1 cut(s) 1392
StyD4I CCNGG 1 cut(s) 824
StyI CCWWGG 7 cut(s) 391, 426, 463, 522, 537, 709, 972
TaaI ACNGT 5 cut(s) 77, 142, 160, 439, 514
TaiI ACGT 4 cut(s) 1012, 1032, 1132, 1344
TaqI TCGA 9 cut(s) 9, 13, 21, 26, 43, 484, 871, 928, 1443
TaqII GACCGA 2 cut(s) 759, 827
TasI AATT 7 cut(s) 207, 254, 313, 337, 802, 1413, 1456
TatI WGTACW 1 cut(s) 70
TauI GCSGC 2 cut(s) 367, 1321
TfiI GAWTC 2 cut(s) 273, 1297
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TscAI CASTG 3 cut(s) 80, 697, 871
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 9 cut(s) 662, 759, 1041, 1216, 1269, 1315, 1321, 1351, 1380
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 1074, 1485
TspRI CASTG 3 cut(s) 80, 697, 871
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 6 cut(s) 207, 254, 313, 337, 802, 1413
XceI RCATGY 2 cut(s) 612, 1310
XhoI CTCGAG 2 cut(s) 483, 870
XmaJI CCTAGG 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 1112
XmnI GAANNNNTTC 1 cut(s) 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.