pycom13g25870

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
22590925 .. 22593548
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g25870.13

Sequence Viewer

Length: 1950 bp
ATGTTACCAGCTACCCAAGAAGAAGCCGCAACAGCATATGACATGGCGGCTATAGAGTACCGTGGGCTCAATGCTGTCACAAACTTTGACCTCAGCCGTTACATCAAATGGCTAAAACCTAATGGTCCTAACAACGACGACAATACCGGTCGGCCTAACCCTATTGCTATTGAGGCTAAATTGCCAAATGTCACTCGAAGCCCTAATGATCAAGGTCGTGGACTTGGCTTCTTTCAGAACCTTGCATACAGTCCCGGAGAAATCATGACGATAGCTCAACCTAGACCAACCACTGCTACATCAGCCCTAGGTCTCCTTCTTCAGTCATCAAAGTTCAAGGAGATGATGGAAATGACAACAGCCACCGATTCCATGCCATCGCAGCCAGAGTTGGGCACGTCACAGTGCACATTTCCCGATGACATACAGACTTACTTTGACTGCCAAGAATCTAGCAGCTATGCTGATGGAGACGACGTTATCTTCAGCGAGCTCAATTCATTCATGCCACCCATGTTCCAGTTTACTCTCTTTTATTTTGTAATTTACTTAAGTAGGTTCTTTCATTTTATTTTATTTTTTTGTGAATACGCAGGAAGGGTCAGCATCCATGTCAAGGATGCACAAAAGATAAAAAAAAAAATTGAAAGAGGAAATTTCACTACTGATCATCATCATGTGGGAGCAAGAGTAAAGAAGAATAAAATTGATTCATCATTTTCTTATATCAATAATAAGCATCATTCTCTCTACTTGGTGTTTCATTTCCTCTCTTTTTTTCATTTTATACCTTTGAAGAATGGCGTGTGCTATCCATACTCCCTTTTTACTTCTCACACATCTCTTGTTAATTTATGTCTTTTGATCTTTTTTAATTCATGTGCGTATGAAGTAAAAAGAGGTGTGTGGATATCACATCTCTTGAAGAATATCTTTGTGAAAATAAAGGGAGGAGAGGAGAGGTGTTATGAAGTAAAAAAAAATTGTCGAAAAGAGGATCATTTGAGTATCTCATGCAATAAAATTGAAATTAAAAGGGGAACCAAAATAAATGGAGGAGAGTTATTTTATACCATTGAAGAACCCTTTTTTCACAAAGGTAATCGCCCAAAGTGGGGTCTTCATTCCATTGCTCTTCTTGTTCCTTGTTGGTTGATTTCAATTGGATATCCAAGTATCAAAAGAATGGGACCGTTAAGTCTCCAGACCAAAGAGCAGATTGAATCTTTTCCTTTGTGGGTGACTCTCTCTTTTCCCTCTACACTGAATCTACCACTAATACCAAGTAGCTTCTCACTTACCTATCTATTGATTAATTCAATTGACGTTTTAGCTCGTATATCTCTACATGAATCTTTACGTTTTATGGAAATCTTTGACAATCATGCAAACCCCAGTAGTATGTTATTTACCCCAAAAGAATCACAAGTCATAACCACAAGAAGCATGCCTCAAATTAGTTTTGGGTGGTCAAACATCACGAGAATTTATAAGTTTATACGCATAGTGATTAAGAATCCAACATCAAAAGGGATACCGTTTCATCATGCCCTAGCAAAGGTGTCTAGTTACATACTCATAACAATAACAATAAAAGTCATGAAAAAGAACAATCATACCCAAAATAACAACGACGATGTGCTCCTAAACCCTATTCTCTCTTTTGGACAAATTTGTATAAAATTTTTAAAAGGTCTGGAAAGACTCCCTCTTGGCTTGGGAAACACTTCAGTCACTTTGAATTCCAAATTTGGGGTTGTTACACCTTCCATAAAGTTACGGAAAAATAGAAAGTTGAATTTCATTCTTCCACAATTGCCCTATTTTGATGAGAATGTAACTTTCGTGAAATTGATTTGCAAAACTAGAATATGTTTGGCAAACTCCATGGAAAAATATGAAAATTCAGACACAATGCTTTTCAGCCTTGTACATTCCTTCTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

650

Amino Acids

74.07

Weight (kDa)

9.23

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1491
AasI GACNNNNNNGTC 1 cut(s) 1197
AciI CCGC 2 cut(s) 27, 47
AclWI GGATC 1 cut(s) 1005
AcsI RAATTY 8 cut(s) 655, 1485, 1671, 1682, 1741, 1748, 1798, 1903
AcuI CTGAAG 3 cut(s) 305, 469, 1713
AfaI GTAC 2 cut(s) 59, 1932
AfiI CCNNNNNNNGG 6 cut(s) 392, 616, 1114, 1115, 1558, 1752
AflII CTTAAG 1 cut(s) 550
AgeI ACCGGT 1 cut(s) 146
AjiI CACGTC 1 cut(s) 399
AluBI AGCT 6 cut(s) 11, 275, 459, 493, 1290, 1334
AluI AGCT 6 cut(s) 11, 275, 459, 493, 1290, 1334
Alw21I GWGCWC 3 cut(s) 410, 495, 1644
Alw26I GTCTC 3 cut(s) 317, 465, 1205
Alw44I GTGCAC 1 cut(s) 406
AlwI GGATC 1 cut(s) 1005
AoxI GGCC 1 cut(s) 152
ApaLI GTGCAC 1 cut(s) 406
ApeKI GCWGC 2 cut(s) 382, 456
ApoI RAATTY 8 cut(s) 655, 1485, 1671, 1682, 1741, 1748, 1798, 1903
AseI ATTAAT 1 cut(s) 1314
AsiGI ACCGGT 1 cut(s) 146
Asp700I GAANNNNTTC 2 cut(s) 1227, 1726
AspA2I CCTAGG 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 125, 1190
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 1252
AvaII GGWCC 2 cut(s) 125, 1190
AvrII CCTAGG 1 cut(s) 307
BaeGI GKGCMC 2 cut(s) 398, 410
BanII GRGCYC 2 cut(s) 69, 495
BauI CACGAG 1 cut(s) 1480
BbsI GAAGAC 1 cut(s) 1112
Bbv12I GWGCWC 3 cut(s) 410, 495, 1644
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 2 cut(s) 394, 468
BccI CCATC 3 cut(s) 340, 385, 461
BceAI ACGGC 1 cut(s) 81
BciVI GTATCC 1 cut(s) 1527
BclI TGATCA 2 cut(s) 208, 667
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 3 cut(s) 317, 465, 1205
BfaI CTAG 6 cut(s) 282, 308, 453, 1553, 1566, 1866
BfmI CTRYAG 1 cut(s) 51
BfrI CTTAAG 1 cut(s) 550
BfuI GTATCC 1 cut(s) 1527
BisI GCNGC 4 cut(s) 27, 48, 383, 457
BlnI CCTAGG 1 cut(s) 307
BlsI GCNGC 4 cut(s) 28, 49, 384, 458
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 2 cut(s) 125, 1190
BmgBI CACGTC 1 cut(s) 399
BmgT120I GGNCC 2 cut(s) 125, 1190
BmiI GGNNCC 2 cut(s) 1042, 1191
BmrFI CCNGG 1 cut(s) 255
BmrI ACTGGG 1 cut(s) 1389
BmsI GCATC 3 cut(s) 610, 615, 748
BmuI ACTGGG 1 cut(s) 1389
BpiI GAAGAC 1 cut(s) 1112
BpmI CTGGAG 1 cut(s) 1187
Bpu10I CCTNAGC 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 672
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 61, 307, 1887
BsaWI WCCGGW 1 cut(s) 146
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 675, 705
Bsc4I CCNNNNNNNGG 6 cut(s) 392, 616, 1114, 1115, 1558, 1752
Bse118I RCCGGY 1 cut(s) 146
Bse1I ACTGG 2 cut(s) 520, 1395
Bse3DI GCAATG 1 cut(s) 1128
Bse8I GATNNNNATC 1 cut(s) 672
BseDI CCNNGG 3 cut(s) 61, 307, 1887
BseGI GGATG 2 cut(s) 606, 625
BseJI GATNNNNATC 1 cut(s) 672
BseLI CCNNNNNNNGG 6 cut(s) 392, 616, 1114, 1115, 1558, 1752
BseMI GCAATG 1 cut(s) 1128
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 2 cut(s) 520, 1395
BseRI GAGGAG 3 cut(s) 966, 971, 1071
BseSI GKGCMC 2 cut(s) 398, 410
BseXI GCAGC 2 cut(s) 394, 468
Bsh1285I CGRYCG 1 cut(s) 151
BshFI GGCC 1 cut(s) 154
BshTI ACCGGT 1 cut(s) 146
BsiEI CGRYCG 1 cut(s) 151
BsiHKAI GWGCWC 3 cut(s) 410, 495, 1644
BsiSI CCGG 2 cut(s) 147, 255
BslFI GGGAC 2 cut(s) 237, 1203
BslI CCNNNNNNNGG 6 cut(s) 392, 616, 1114, 1115, 1558, 1752
BsmAI GTCTC 3 cut(s) 317, 465, 1205
BsmBI CGTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 237, 1203
BsnI GGCC 1 cut(s) 154
Bso31I GGTCTC 1 cut(s) 317
Bsp1286I GDGCHC 5 cut(s) 69, 398, 410, 495, 1644
Bsp1407I TGTACA 1 cut(s) 1930
Bsp143I GATC 4 cut(s) 208, 667, 864, 997
Bsp19I CCATGG 1 cut(s) 1887
BspACI CCGC 2 cut(s) 27, 47
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 1 cut(s) 105
BspHI TCATGA 2 cut(s) 264, 1599
BspLI GGNNCC 2 cut(s) 1042, 1191
BspPI GGATC 1 cut(s) 1005
BspQI GCTCTTC 1 cut(s) 1140
BspTI CTTAAG 1 cut(s) 550
BspTNI GGTCTC 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 1128
BsrFI RCCGGY 1 cut(s) 146
BsrGI TGTACA 1 cut(s) 1930
BsrI ACTGG 2 cut(s) 520, 1395
BssAI RCCGGY 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 61, 307, 1887
BssMI GATC 4 cut(s) 208, 667, 864, 997
BssSI CACGAG 1 cut(s) 1480
BssT1I CCWWGG 2 cut(s) 307, 1887
Bst2BI CACGAG 1 cut(s) 1480
Bst4CI ACNGT 5 cut(s) 62, 251, 405, 1194, 1539
Bst6I CTCTTC 1 cut(s) 1140
BstAFI CTTAAG 1 cut(s) 550
BstAUI TGTACA 1 cut(s) 1930
BstC8I GCNNGC 2 cut(s) 491, 1448
BstDEI CTNAG 1 cut(s) 92
BstDSI CCRYGG 2 cut(s) 61, 1887
BstENI CCTNNNNNAGG 1 cut(s) 1556
BstF5I GGATG 2 cut(s) 606, 625
BstKTI GATC 4 cut(s) 211, 670, 867, 1000
BstMAI GTCTC 3 cut(s) 317, 465, 1205
BstMBI GATC 4 cut(s) 208, 667, 864, 997
BstMCI CGRYCG 1 cut(s) 151
BstMWI GCNNNNNNNGC 4 cut(s) 32, 173, 302, 382
BstNSI RCATGY 1 cut(s) 1450
BstSCI CCNGG 1 cut(s) 253
BstSFI CTRYAG 1 cut(s) 51
BstSLI GKGCMC 2 cut(s) 398, 410
BstV1I GCAGC 2 cut(s) 394, 468
BstV2I GAAGAC 1 cut(s) 1112
BsuI GTATCC 1 cut(s) 1527
BsuRI GGCC 1 cut(s) 154
BtgI CCRYGG 2 cut(s) 61, 1887
BtgZI GCGATG 1 cut(s) 363
BtrI CACGTC 1 cut(s) 399
BtsCI GGATG 2 cut(s) 606, 625
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 3 cut(s) 291, 410, 1262
Cac8I GCNNGC 2 cut(s) 491, 1448
CciI TCATGA 2 cut(s) 264, 1599
Cfr10I RCCGGY 1 cut(s) 146
Cfr13I GGNCC 2 cut(s) 125, 1190
Csp6I GTAC 2 cut(s) 58, 1931
CspAI ACCGGT 1 cut(s) 146
CviQI GTAC 2 cut(s) 58, 1931
DdeI CTNAG 1 cut(s) 92
DpnI GATC 4 cut(s) 210, 669, 866, 999
DpnII GATC 4 cut(s) 208, 667, 864, 997
DraI TTTAAA 1 cut(s) 1689
DrdI GACNNNNNNGTC 1 cut(s) 1197
DseDI GACNNNNNNGTC 1 cut(s) 1197
Eam1104I CTCTTC 1 cut(s) 1140
EarI CTCTTC 1 cut(s) 1140
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 2 cut(s) 307, 1887
Eco24I GRGCYC 2 cut(s) 69, 495
Eco31I GGTCTC 1 cut(s) 317
Eco32I GATATC 2 cut(s) 912, 1169
Eco47I GGWCC 2 cut(s) 125, 1190
Eco53kI GAGCTC 1 cut(s) 493
Eco57I CTGAAG 3 cut(s) 305, 469, 1713
EcoICRI GAGCTC 1 cut(s) 493
EcoNI CCTNNNNNAGG 1 cut(s) 1556
EcoRI GAATTC 1 cut(s) 1741
EcoRV GATATC 2 cut(s) 912, 1169
EcoT14I CCWWGG 2 cut(s) 307, 1887
EcoT38I GRGCYC 2 cut(s) 69, 495
ErhI CCWWGG 2 cut(s) 307, 1887
Esp3I CGTCTC 1 cut(s) 465
FalI AAGNNNNNCTT 2 cut(s) 917, 949
FaqI GGGAC 2 cut(s) 237, 1203
FauNDI CATATG 1 cut(s) 37
FbaI TGATCA 2 cut(s) 208, 667
Fnu4HI GCNGC 4 cut(s) 27, 48, 383, 457
FokI GGATG 2 cut(s) 593, 632
FriOI GRGCYC 2 cut(s) 69, 495
Fsp4HI GCNGC 4 cut(s) 27, 48, 383, 457
FspBI CTAG 6 cut(s) 282, 308, 453, 1553, 1566, 1866
GluI GCNGC 4 cut(s) 27, 48, 383, 457
GsuI CTGGAG 1 cut(s) 1187
HaeIII GGCC 1 cut(s) 154
HapII CCGG 2 cut(s) 147, 255
HpaII CCGG 2 cut(s) 147, 255
HphI GGTGA 1 cut(s) 1252
Hpy166II GTNNAC 3 cut(s) 221, 408, 525
Hpy188I TCNGA 2 cut(s) 237, 1909
Hpy188III TCNNGA 8 cut(s) 265, 416, 922, 1204, 1480, 1600, 1697, 1846
Hpy8I GTNNAC 3 cut(s) 221, 408, 525
Hpy99I CGWCG 3 cut(s) 140, 479, 1637
HpyAV CCTTC 4 cut(s) 326, 591, 1776, 1948
HpyCH4III ACNGT 5 cut(s) 62, 251, 405, 1194, 1539
HpyCH4IV ACGT 4 cut(s) 398, 477, 1326, 1360
HpyCH4V TGCA 6 cut(s) 245, 408, 623, 1017, 1388, 1860
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 173, 302, 382
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 4 cut(s) 398, 477, 1326, 1360
Ksp22I TGATCA 2 cut(s) 208, 667
Kzo9I GATC 4 cut(s) 208, 667, 864, 997
LguI GCTCTTC 1 cut(s) 1140
LmnI GCTCC 2 cut(s) 683, 1647
LpnPI CCDG 9 cut(s) 21, 160, 268, 399, 533, 579, 1217, 1408, 1682
Lsp1109I GCAGC 2 cut(s) 394, 468
LweI GCATC 3 cut(s) 610, 615, 748
MaeI CTAG 6 cut(s) 282, 308, 453, 1553, 1566, 1866
MaeII ACGT 4 cut(s) 398, 477, 1326, 1360
MalI GATC 4 cut(s) 210, 669, 866, 999
MboI GATC 4 cut(s) 208, 667, 864, 997
MfeI CAATTG 3 cut(s) 1161, 1320, 1814
MhlI GDGCHC 5 cut(s) 69, 398, 410, 495, 1644
MlyI GAGTC 2 cut(s) 1237, 1698
MmeI TCCRAC 1 cut(s) 1544
MroXI GAANNNNTTC 2 cut(s) 1227, 1726
MseI TTAA 8 cut(s) 551, 849, 873, 1032, 1196, 1314, 1512, 1688
MslI CAYNNNNRTG 1 cut(s) 675
MspCI CTTAAG 1 cut(s) 550
MspI CCGG 2 cut(s) 147, 255
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 3 cut(s) 1161, 1320, 1814
MwoI GCNNNNNNNGC 4 cut(s) 32, 173, 302, 382
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 1887
NdeI CATATG 1 cut(s) 37
NdeII GATC 4 cut(s) 208, 667, 864, 997
NlaIV GGNNCC 2 cut(s) 1042, 1191
NmuCI GTSAC 5 cut(s) 76, 190, 399, 1240, 1732
NspI RCATGY 1 cut(s) 1450
PaeI GCATGC 1 cut(s) 1450
PagI TCATGA 2 cut(s) 264, 1599
PciSI GCTCTTC 1 cut(s) 1140
PdmI GAANNNNTTC 2 cut(s) 1227, 1726
PfeI GAWTC 8 cut(s) 368, 449, 710, 1223, 1267, 1352, 1421, 1516
PfoI TCCNGGA 1 cut(s) 253
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 4 cut(s) 28, 49, 384, 458
PleI GAGTC 2 cut(s) 1237, 1698
PpsI GAGTC 2 cut(s) 1237, 1698
PshBI ATTAAT 1 cut(s) 1314
PsiI TTATAA 1 cut(s) 1491
Psp124BI GAGCTC 1 cut(s) 495
PspN4I GGNNCC 2 cut(s) 1042, 1191
PspPI GGNCC 2 cut(s) 125, 1190
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
RsaI GTAC 2 cut(s) 59, 1932
RsaNI GTAC 2 cut(s) 58, 1931
RseI CAYNNNNRTG 1 cut(s) 675
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 1140
SaqAI TTAA 8 cut(s) 551, 849, 873, 1032, 1196, 1314, 1512, 1688
SatI GCNGC 4 cut(s) 27, 48, 383, 457
Sau3AI GATC 4 cut(s) 208, 667, 864, 997
Sau96I GGNCC 2 cut(s) 125, 1190
SchI GAGTC 2 cut(s) 1237, 1698
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 5 cut(s) 69, 398, 410, 495, 1644
SfaNI GCATC 3 cut(s) 610, 615, 748
SfcI CTRYAG 1 cut(s) 51
SinI GGWCC 2 cut(s) 125, 1190
SmiMI CAYNNNNRTG 1 cut(s) 675
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
SphI GCATGC 1 cut(s) 1450
SsiI CCGC 2 cut(s) 27, 47
SspMI CTAG 6 cut(s) 282, 308, 453, 1553, 1566, 1866
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 2 cut(s) 307, 1887
TaaI ACNGT 5 cut(s) 62, 251, 405, 1194, 1539
TaiI ACGT 4 cut(s) 401, 480, 1329, 1363
TaqI TCGA 2 cut(s) 196, 988
TatI WGTACW 1 cut(s) 1930
TauI GCSGC 2 cut(s) 29, 50
TfiI GAWTC 8 cut(s) 368, 449, 710, 1223, 1267, 1352, 1421, 1516
Tru1I TTAA 8 cut(s) 551, 849, 873, 1032, 1196, 1314, 1512, 1688
Tru9I TTAA 8 cut(s) 551, 849, 873, 1032, 1196, 1314, 1512, 1688
TscAI CASTG 3 cut(s) 298, 410, 1269
TseFI GTSAC 5 cut(s) 76, 190, 399, 1240, 1732
TseI GCWGC 2 cut(s) 382, 456
Tsp45I GTSAC 5 cut(s) 76, 190, 399, 1240, 1732
TspGWI ACGGA 1 cut(s) 1795
TspRI CASTG 3 cut(s) 298, 410, 1269
Vha464I CTTAAG 1 cut(s) 550
VneI GTGCAC 1 cut(s) 406
VpaK11BI GGWCC 2 cut(s) 125, 1190
VspI ATTAAT 1 cut(s) 1314
XagI CCTNNNNNAGG 1 cut(s) 1556
XapI RAATTY 8 cut(s) 655, 1485, 1671, 1682, 1741, 1748, 1798, 1903
XceI RCATGY 1 cut(s) 1450
XmaJI CCTAGG 1 cut(s) 307
XmnI GAANNNNTTC 2 cut(s) 1227, 1726
XspI CTAG 6 cut(s) 282, 308, 453, 1553, 1566, 1866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.