pycom13g26230

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
22933269 .. 22934433
1165 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 837 bp
ATGCCCATCGTTCTAGAATCATTGAACATGCGATCTGGAAGGGGCATGAACACTCTCCCTAAGATCAAGTTTTTCTTCTATCCATTTCTGTCTCGGTGCCAATCCCCTCTACCCCATGTGTCCCTGGACCTATGCGGTCTGCTTTGCTCCAGATTAAACTATCGCATTGATCGTCAAGCAATAACTATTTTTGATTGCTGGTTGCTTGCTCTTTCTAAGGTCATCGACCGAAACGGTGCTGCTTTGAATAGGGTATTAACTTTGGCTCTCTTTATCTACTTAGAAATCTGGAAAATCCGTCATTGCAGTTTTAACAACTTGGTGCCTTCCTCTTATAGAACTTTGAAGCTGTTCAGACCCCCATCCGTTCTCGTTCATATTTTGTCCATCGACGGTCTTCACTTACGGCGACTAAAGCTACGCTTGAAGTCTCGATCACTTGTGCTCTCTAAGCCTAAAGCCTGTTTTCCTTTGTTTTCCCTGGAGTTTCCTCCGTCTCTAATGAAATCTTATTTCGACAAAAAAAAAAAAAAAAAAAAGAATTTAGCCACTCTCTCTCTCTTCATCATCATGGCCATCAGACGCACAATGACACAGACTAAAAAAGGAAAGAAAAGTCTTATACATCCCGCAAAAGACCAGCAAGAACCAGTTTCAAAGTTAAATTATAAAAAAAACAGAGAAAAGCCTTTTGATGTGGAAAAGAAACAAAGGAGAGGGAGGCTGAGAGACTTTCTCTTCTTGCTGCTTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCTCTCTCTCCCTTTGGTTGTAGCAGCAGCAGCTGCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

279

Amino Acids

31.98

Weight (kDa)

10.61

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 669
AccB1I GGYRCC 2 cut(s) 96, 322
AciI CCGC 2 cut(s) 135, 630
AcoI YGGCCR 1 cut(s) 573
AcsI RAATTY 1 cut(s) 541
AgsI TTSAA 5 cut(s) 25, 247, 346, 427, 657
AjnI CCWGG 2 cut(s) 123, 480
AluBI AGCT 3 cut(s) 349, 418, 827
AluI AGCT 3 cut(s) 349, 418, 827
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 4 cut(s) 96, 435, 501, 723
AlwNI CAGNNNCTG 1 cut(s) 827
AoxI GGCC 1 cut(s) 573
ApeKI GCWGC 6 cut(s) 239, 745, 818, 821, 824, 827
ApoI RAATTY 1 cut(s) 541
Asp700I GAANNNNTTC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 127
AvaII GGWCC 1 cut(s) 127
BalI TGGCCA 1 cut(s) 575
BanI GGYRCC 2 cut(s) 96, 322
BbsI GAAGAC 1 cut(s) 389
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 5 cut(s) 226, 732, 814, 830, 833
BccI CCATC 4 cut(s) 14, 370, 395, 584
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 2 cut(s) 125, 482
BcoDI GTCTC 4 cut(s) 96, 435, 501, 723
BfaI CTAG 1 cut(s) 14
BisI GCNGC 6 cut(s) 240, 746, 819, 822, 825, 828
BlsI GCNGC 6 cut(s) 241, 747, 820, 823, 826, 829
Bme1390I CCNGG 2 cut(s) 125, 482
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 98, 324
BmrFI CCNGG 2 cut(s) 125, 482
BpiI GAAGAC 1 cut(s) 389
BplI GAGNNNNNCTC 2 cut(s) 720, 752
BpmI CTGGAG 2 cut(s) 133, 503
BsaJI CCNNGG 2 cut(s) 123, 480
Bse1I ACTGG 1 cut(s) 650
Bse3DI GCAATG 1 cut(s) 301
BseBI CCWGG 2 cut(s) 125, 482
BseDI CCNNGG 2 cut(s) 123, 480
BseGI GGATG 2 cut(s) 362, 625
BseMI GCAATG 1 cut(s) 301
BseMII CTCAG 1 cut(s) 716
BseNI ACTGG 1 cut(s) 650
BseXI GCAGC 5 cut(s) 226, 732, 814, 830, 833
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 1 cut(s) 575
BshNI GGYRCC 2 cut(s) 96, 322
BsiEI CGRYCG 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 447
BslFI GGGAC 1 cut(s) 106
BsmAI GTCTC 4 cut(s) 96, 435, 501, 723
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 1 cut(s) 575
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 4 cut(s) 32, 63, 169, 434
BspACI CCGC 2 cut(s) 135, 630
BspANI GGCC 1 cut(s) 575
BspCNI CTCAG 1 cut(s) 717
BspLI GGNNCC 2 cut(s) 98, 324
BspT107I GGYRCC 2 cut(s) 96, 322
BsrDI GCAATG 1 cut(s) 301
BsrI ACTGG 1 cut(s) 650
BssECI CCNNGG 2 cut(s) 123, 480
BssMI GATC 4 cut(s) 32, 63, 169, 434
Bst2UI CCWGG 2 cut(s) 125, 482
Bst4CI ACNGT 2 cut(s) 236, 395
Bst6I CTCTTC 2 cut(s) 566, 743
BstAPI GCANNNNNTGC 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 5 cut(s) 60, 216, 280, 450, 725
BstF5I GGATG 2 cut(s) 362, 625
BstKTI GATC 4 cut(s) 35, 66, 172, 437
BstMAI GTCTC 4 cut(s) 96, 435, 501, 723
BstMBI GATC 4 cut(s) 32, 63, 169, 434
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 4 cut(s) 415, 451, 824, 827
BstNI CCWGG 2 cut(s) 125, 482
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 123, 480
BstV1I GCAGC 5 cut(s) 226, 732, 814, 830, 833
BstV2I GAAGAC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 575
BtsCI GGATG 2 cut(s) 362, 625
Cac8I GCNNGC 1 cut(s) 207
CaiI CAGNNNCTG 1 cut(s) 827
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 1 cut(s) 591
CviAII CATG 4 cut(s) 28, 46, 116, 571
DdeI CTNAG 5 cut(s) 60, 216, 280, 450, 725
DpnI GATC 4 cut(s) 34, 65, 171, 436
DpnII GATC 4 cut(s) 32, 63, 169, 434
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 2 cut(s) 566, 743
EarI CTCTTC 2 cut(s) 566, 743
Eco47I GGWCC 1 cut(s) 127
EcoRII CCWGG 2 cut(s) 123, 480
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 4 cut(s) 31, 49, 119, 574
FaiI YATR 9 cut(s) 29, 47, 117, 133, 336, 378, 572, 623, 669
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 407, 439
FaqI GGGAC 1 cut(s) 106
FatI CATG 4 cut(s) 27, 45, 115, 570
FauI CCCGC 1 cut(s) 637
Fnu4HI GCNGC 6 cut(s) 240, 746, 819, 822, 825, 828
FokI GGATG 2 cut(s) 349, 612
Fsp4HI GCNGC 6 cut(s) 240, 746, 819, 822, 825, 828
FspBI CTAG 1 cut(s) 14
GluI GCNGC 6 cut(s) 240, 746, 819, 822, 825, 828
GsuI CTGGAG 2 cut(s) 133, 503
HaeIII GGCC 1 cut(s) 575
HgaI GACGC 1 cut(s) 591
Hin1II CATG 4 cut(s) 31, 49, 119, 574
HinfI GANTC 1 cut(s) 17
Hpy188I TCNGA 2 cut(s) 356, 581
Hpy188III TCNNGA 5 cut(s) 14, 36, 150, 289, 432
Hpy99I CGWCG 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 33, 336
HpyCH4III ACNGT 2 cut(s) 236, 395
HpyCH4V TGCA 1 cut(s) 306
HpyF10VI GCNNNNNNNGC 4 cut(s) 415, 451, 824, 827
HpyF3I CTNAG 5 cut(s) 60, 216, 280, 450, 725
Hsp92II CATG 4 cut(s) 31, 49, 119, 574
Kzo9I GATC 4 cut(s) 32, 63, 169, 434
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 5 cut(s) 226, 732, 814, 830, 833
MaeI CTAG 1 cut(s) 14
MalI GATC 4 cut(s) 34, 65, 171, 436
MboI GATC 4 cut(s) 32, 63, 169, 434
MboII GAAGA 4 cut(s) 67, 389, 553, 730
MhlI GDGCHC 1 cut(s) 447
MlsI TGGCCA 1 cut(s) 575
MluCI AATT 2 cut(s) 541, 664
MluNI TGGCCA 1 cut(s) 575
MnlI CCTC 7 cut(s) 117, 340, 501, 710, 714, 762, 806
Mox20I TGGCCA 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 1 cut(s) 575
MseI TTAA 5 cut(s) 155, 257, 312, 662, 835
MslI CAYNNNNRTG 1 cut(s) 569
Msp20I TGGCCA 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 827
MspR9I CCNGG 2 cut(s) 125, 482
MvaI CCWGG 2 cut(s) 125, 482
MwoI GCNNNNNNNGC 4 cut(s) 415, 451, 824, 827
NdeII GATC 4 cut(s) 32, 63, 169, 434
NlaIII CATG 4 cut(s) 31, 49, 119, 574
NlaIV GGNNCC 2 cut(s) 98, 324
NspI RCATGY 1 cut(s) 31
PcsI WCGNNNNNNNCGW 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 6 cut(s) 241, 747, 820, 823, 826, 829
PsiI TTATAA 1 cut(s) 669
Psp6I CCWGG 2 cut(s) 123, 480
PspGI CCWGG 2 cut(s) 123, 480
PspN4I GGNNCC 2 cut(s) 98, 324
PspPI GGNCC 1 cut(s) 127
PstNI CAGNNNCTG 1 cut(s) 827
PvuII CAGCTG 1 cut(s) 827
RseI CAYNNNNRTG 1 cut(s) 569
SaqAI TTAA 5 cut(s) 155, 257, 312, 662, 835
SatI GCNGC 6 cut(s) 240, 746, 819, 822, 825, 828
Sau3AI GATC 4 cut(s) 32, 63, 169, 434
Sau96I GGNCC 1 cut(s) 127
ScrFI CCNGG 2 cut(s) 125, 482
SduI GDGCHC 1 cut(s) 447
SetI ASST 5 cut(s) 132, 222, 351, 420, 829
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 569
Sse9I AATT 2 cut(s) 541, 664
SsiI CCGC 2 cut(s) 135, 630
SspMI CTAG 1 cut(s) 14
StyD4I CCNGG 2 cut(s) 123, 480
TaaI ACNGT 2 cut(s) 236, 395
TaqI TCGA 4 cut(s) 225, 390, 433, 516
TaqII GACCGA 1 cut(s) 243
TasI AATT 2 cut(s) 541, 664
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 5 cut(s) 155, 257, 312, 662, 835
Tru9I TTAA 5 cut(s) 155, 257, 312, 662, 835
TseI GCWGC 6 cut(s) 239, 745, 818, 821, 824, 827
TspDTI ATGAA 4 cut(s) 62, 365, 518, 553
TspGWI ACGGA 3 cut(s) 287, 355, 483
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 1 cut(s) 541
XbaI TCTAGA 1 cut(s) 13
XceI RCATGY 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.