pycom13g27550

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
N/A
Physical Location & Seq
Forward (+)
23761970 .. 23764306
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGTGGGGAGGGAGAGGGTTCGTTGTTTGGGTTTGTCTTTGGTTATTGGTGTCAGCAAAGTCTAGGAAGAGGTTTGCTGTTGCAAATTGCGACCATGAAATAGACATGTTCATCCTTTCTCGTTGGATGTGTGGGCACACGAGAAAGGATAAGATTAGGAATGAGGATATCCAAGGTAAAGTAGGAGTAGCCGAAATTGAAGGAAAGATGAGAGAAAATCGTTTACGGTGGGTTGGACATGTGAAACAAAGGTCTACTGATGCTTTGGTTAGAAGATGCGATTATGGGATAGAGACTCTAAAAAAAGACTTAGATTCATATAGCCAACCCTACTTAGTGGGAAAAGGCTTCGTTGTTGTTCGTCAGATCATATGGCATGTTTTTCAGTTTATGACAAAAGAAGACTTTGTTGAAGGGTTTTATCTTCTTTTTCTTCCCATCGGTATGCTTCTACCATGCAGGCTTATTTTCTTCTGTCTTTTTGAGACTGACTTTAGGGCCTTTCAGCTGTTTCTTCGAACTCTGAATCCTTTTGGTTTCTCTGGGAATAATGTGACGACTTGTTTAGTTCAGCTCCCATCCATCCGATTCGATTCTATGCAGTGTCTTGGTGTTCTCTATGAAACAAAACTAGTAAATGTTTTAACACTACCAGAAAAATGCGTTATAAAAACCTTGTACTTCAATTTTCTTAGGATCCCGGAGGTTATACACCACCCTCGTTTGTTTTTACATTGTTATCCGTCTATGATAGAAACCACCTACCGTTGCTGCTTCAACCTTACAATTGCAGCTATGGTAGAAATGGATGAAGGAAGGAGACGGACTGGACATTCCACTAACAAACTATCTTTACTAAAGCACTCCCCCTTGAAGAACAAGCTTGTCCTCAAGGCTATTAAATCGTACTCGTGCAACAGCACCCTTCTTGTACTTTCCCTTGGCAAAGATTGCCAAATGAGACTCCTGCATTTTCCCATTATCAGTTACTTGGGGCAATGCTGTACTAGGCTGTACCAGACTTCCAAGGTCTTTCTTAAGGCAATTTTCCTGCAAAAGACTTCAGGTAGGTTTCAAGACATCTATTTACCACCTCAATTTGCCAATCACTCTGTGGATGGTAACCAAGACTCATCACTAAGGAATATTGGATCTTTGTCACTAACAATGGTAGAGAGCTTCCCATGTAGTTTAAACACGTTCATACTTCGTGAGCCTATCAACCACAACCATTATAACCGATTTACCCTGACATGTGGCAGCCCAACAATGAAATCCATACTGACATCATGGCAACCCAACAATGAAATCCATACTGACATCAGTCCAAATTTACTGTGGAATAGGAAATGGTTGAATCAAACCTGGAGGAGAAGGAGTTTCATACTTATTCTGTTGGCAAGTGTTGCATTTAGAAATGAAGGCTTTGATGTCTTTCTTCAATCCAGGCCAATAGAAGCCCCTTGTGATCTTGTGATAAGTCTTTTGTACACCTTGATGGCCAGCAATTGGGGTGGAAGGGTGTTCTTCCAATATGTTCAGCTTCCAAAGAGAGTTAGGACTAATCACAATCCTATTTTTATATTTCAAGAAGCCATTGTCCATTTGGTAATGAGACTAAGTAGGTTCCCCAGAAGGATAGACTTTTCTTCTTCACAATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

555

Amino Acids

64.52

Weight (kDa)

9.59

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 668, 1236
AccB7I CCANNNNNTGG 1 cut(s) 1509
AccI GTMKAC 1 cut(s) 254
AclWI GGATC 3 cut(s) 691, 704, 1159
AcoI YGGCCR 1 cut(s) 1500
AcsI RAATTY 1 cut(s) 1330
AcuI CTGAAG 1 cut(s) 1047
AdeI CACNNNGTG 1 cut(s) 1114
AfaI GTAC 6 cut(s) 680, 908, 933, 1006, 1016, 1490
AfiI CCNNNNNNNGG 1 cut(s) 1509
AflII CTTAAG 1 cut(s) 1037
AflIII ACRYGT 4 cut(s) 105, 238, 1197, 1253
AgsI TTSAA 9 cut(s) 200, 413, 685, 778, 874, 1076, 1357, 1442, 1589
AhlI ACTAGT 1 cut(s) 631
AjnI CCWGG 2 cut(s) 1364, 1445
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
AluBI AGCT 6 cut(s) 508, 574, 794, 883, 1179, 1543
AluI AGCT 6 cut(s) 508, 574, 794, 883, 1179, 1543
Alw26I GTCTC 5 cut(s) 287, 479, 814, 955, 1609
AlwI GGATC 3 cut(s) 691, 704, 1159
AoxI GGCC 3 cut(s) 498, 1448, 1500
ApeKI GCWGC 3 cut(s) 771, 791, 1260
ApoI RAATTY 1 cut(s) 1330
AspS9I GGNCC 1 cut(s) 498
AsuC2I CCSGG 1 cut(s) 701
AsuII TTCGAA 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 138
BalI TGGCCA 1 cut(s) 1502
BamHI GGATCC 1 cut(s) 696
BauI CACGAG 2 cut(s) 139, 910
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 3 cut(s) 758, 803, 1272
BccI CCATC 5 cut(s) 446, 586, 590, 1112, 1492
BciT130I CCWGG 2 cut(s) 1366, 1447
BcnI CCSGG 1 cut(s) 701
BcoDI GTCTC 5 cut(s) 287, 479, 814, 955, 1609
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 3 cut(s) 63, 632, 1008
BfrI CTTAAG 1 cut(s) 1037
BisI GCNGC 3 cut(s) 772, 792, 1261
BlsI GCNGC 3 cut(s) 773, 793, 1262
Bme1390I CCNGG 3 cut(s) 701, 1366, 1447
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 2 cut(s) 698, 1628
BmrFI CCNGG 3 cut(s) 701, 1366, 1447
BmsI GCATC 2 cut(s) 250, 266
BoxI GACNNNNGTC 1 cut(s) 1323
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 1387
Bpu14I TTCGAA 1 cut(s) 517
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 701
BsaJI CCNNGG 3 cut(s) 172, 940, 1026
BsaXI ACNNNNNCTCC 2 cut(s) 1363, 1393
Bsc4I CCNNNNNNNGG 1 cut(s) 1509
Bse1I ACTGG 1 cut(s) 832
Bse3DI GCAATG 1 cut(s) 1004
BseBI CCWGG 2 cut(s) 1366, 1447
BseDI CCNNGG 3 cut(s) 172, 940, 1026
BseGI GGATG 6 cut(s) 111, 132, 578, 582, 814, 1123
BseLI CCNNNNNNNGG 1 cut(s) 1509
BseMI GCAATG 1 cut(s) 1004
BseNI ACTGG 1 cut(s) 832
BseRI GAGGAG 1 cut(s) 1384
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 3 cut(s) 758, 803, 1272
BshFI GGCC 3 cut(s) 500, 1450, 1502
BsiSI CCGG 1 cut(s) 701
BslI CCNNNNNNNGG 1 cut(s) 1509
BsmAI GTCTC 5 cut(s) 287, 479, 814, 955, 1609
BsmBI CGTCTC 1 cut(s) 814
BsnI GGCC 3 cut(s) 500, 1450, 1502
Bsp119I TTCGAA 1 cut(s) 517
Bsp1286I GDGCHC 1 cut(s) 138
Bsp1407I TGTACA 1 cut(s) 1488
Bsp143I GATC 4 cut(s) 366, 696, 1151, 1468
BspANI GGCC 3 cut(s) 500, 1450, 1502
BspLI GGNNCC 2 cut(s) 698, 1628
BspPI GGATC 3 cut(s) 691, 704, 1159
BspT104I TTCGAA 1 cut(s) 517
BspTI CTTAAG 1 cut(s) 1037
BsrDI GCAATG 1 cut(s) 1004
BsrGI TGTACA 1 cut(s) 1488
BsrI ACTGG 1 cut(s) 832
BssECI CCNNGG 3 cut(s) 172, 940, 1026
BssMI GATC 4 cut(s) 366, 696, 1151, 1468
BssSI CACGAG 2 cut(s) 139, 910
BssT1I CCWWGG 3 cut(s) 172, 940, 1026
Bst2BI CACGAG 2 cut(s) 139, 910
Bst2UI CCWGG 2 cut(s) 1366, 1447
Bst4CI ACNGT 3 cut(s) 228, 767, 1338
Bst6I CTCTTC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 1037
BstAPI GCANNNNNTGC 2 cut(s) 951, 1406
BstAUI TGTACA 1 cut(s) 1488
BstBI TTCGAA 1 cut(s) 517
BstC8I GCNNGC 2 cut(s) 461, 1504
BstDEI CTNAG 5 cut(s) 310, 334, 692, 1139, 1619
BstEII GGTNACC 1 cut(s) 1121
BstF5I GGATG 6 cut(s) 111, 132, 578, 582, 814, 1123
BstKTI GATC 4 cut(s) 369, 699, 1154, 1471
BstMAI GTCTC 5 cut(s) 287, 479, 814, 955, 1609
BstMBI GATC 4 cut(s) 366, 696, 1151, 1468
BstMWI GCNNNNNNNGC 2 cut(s) 951, 1406
BstNI CCWGG 2 cut(s) 1366, 1447
BstNSI RCATGY 4 cut(s) 109, 242, 380, 1257
BstPAI GACNNNNGTC 1 cut(s) 1323
BstPI GGTNACC 1 cut(s) 1121
BstSCI CCNGG 3 cut(s) 699, 1364, 1445
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 758, 803, 1272
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 2 cut(s) 696, 1151
BstYI RGATCY 2 cut(s) 696, 1151
BsuRI GGCC 3 cut(s) 500, 1450, 1502
BtsCI GGATG 6 cut(s) 111, 132, 578, 582, 814, 1123
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 1 cut(s) 608
Cac8I GCNNGC 2 cut(s) 461, 1504
Cfr13I GGNCC 1 cut(s) 498
Csp6I GTAC 6 cut(s) 679, 907, 932, 1005, 1015, 1489
CspCI CAANNNNNGTGG 2 cut(s) 1495, 1530
CviAII CATG 8 cut(s) 95, 106, 239, 377, 456, 1185, 1254, 1290
CviQI GTAC 6 cut(s) 679, 907, 932, 1005, 1015, 1489
DdeI CTNAG 5 cut(s) 310, 334, 692, 1139, 1619
DpnI GATC 4 cut(s) 368, 698, 1153, 1470
DpnII GATC 4 cut(s) 366, 696, 1151, 1468
DraI TTTAAA 1 cut(s) 1194
DraIII CACNNNGTG 1 cut(s) 1114
EaeI YGGCCR 1 cut(s) 1500
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 3 cut(s) 172, 940, 1026
Eco32I GATATC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 1047
Eco91I GGTNACC 1 cut(s) 1121
EcoO109I RGGNCCY 1 cut(s) 498
EcoO65I GGTNACC 1 cut(s) 1121
EcoRII CCWGG 2 cut(s) 1364, 1445
EcoRV GATATC 1 cut(s) 169
EcoT14I CCWWGG 3 cut(s) 172, 940, 1026
ErhI CCWWGG 3 cut(s) 172, 940, 1026
Esp3I CGTCTC 1 cut(s) 814
FaeI CATG 8 cut(s) 98, 109, 242, 380, 459, 1188, 1257, 1293
FatI CATG 8 cut(s) 94, 105, 238, 376, 455, 1184, 1253, 1289
FauNDI CATATG 1 cut(s) 371
FblI GTMKAC 1 cut(s) 254
Fnu4HI GCNGC 3 cut(s) 772, 792, 1261
FokI GGATG 6 cut(s) 98, 139, 565, 569, 821, 1130
Fsp4HI GCNGC 3 cut(s) 772, 792, 1261
FspBI CTAG 3 cut(s) 63, 632, 1008
GluI GCNGC 3 cut(s) 772, 792, 1261
GsuI CTGGAG 1 cut(s) 1387
HaeIII GGCC 3 cut(s) 500, 1450, 1502
HapII CCGG 1 cut(s) 701
Hin1II CATG 8 cut(s) 98, 109, 242, 380, 459, 1188, 1257, 1293
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 8 cut(s) 295, 314, 526, 588, 593, 963, 1130, 1357
HpaII CCGG 1 cut(s) 701
Hpy166II GTNNAC 3 cut(s) 224, 255, 1491
Hpy188I TCNGA 3 cut(s) 366, 525, 587
Hpy188III TCNNGA 4 cut(s) 1076, 1211, 1589, 1662
Hpy8I GTNNAC 3 cut(s) 224, 255, 1491
HpyAV CCTTC 9 cut(s) 194, 407, 806, 810, 935, 1368, 1415, 1512, 1629
HpyCH4III ACNGT 3 cut(s) 228, 767, 1338
HpyCH4IV ACGT 1 cut(s) 1199
HpyCH4V TGCA 8 cut(s) 83, 459, 601, 791, 915, 970, 1054, 1409
HpyF10VI GCNNNNNNNGC 2 cut(s) 951, 1406
HpyF3I CTNAG 5 cut(s) 310, 334, 692, 1139, 1619
HpySE526I ACGT 1 cut(s) 1199
Hsp92II CATG 8 cut(s) 98, 109, 242, 380, 459, 1188, 1257, 1293
Kzo9I GATC 4 cut(s) 366, 696, 1151, 1468
LmnI GCTCC 1 cut(s) 579
Lsp1109I GCAGC 3 cut(s) 758, 803, 1272
LweI GCATC 2 cut(s) 250, 266
MaeI CTAG 3 cut(s) 63, 632, 1008
MaeII ACGT 1 cut(s) 1199
MaeIII GTNAC 4 cut(s) 553, 986, 1121, 1158
MalI GATC 4 cut(s) 368, 698, 1153, 1470
MboI GATC 4 cut(s) 366, 696, 1151, 1468
MfeI CAATTG 2 cut(s) 786, 1507
MflI RGATCY 2 cut(s) 696, 1151
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 1502
MluCI AATT 8 cut(s) 85, 195, 685, 786, 1044, 1097, 1330, 1507
MluNI TGGCCA 1 cut(s) 1502
MlyI GAGTC 3 cut(s) 289, 957, 1124
MmeI TCCRAC 2 cut(s) 104, 214
MnlI CCTC 8 cut(s) 8, 63, 157, 697, 729, 899, 1104, 1362
Mox20I TGGCCA 1 cut(s) 1502
MscI TGGCCA 1 cut(s) 1502
MseI TTAA 4 cut(s) 644, 900, 1038, 1193
MslI CAYNNNNRTG 2 cut(s) 443, 1496
Msp20I TGGCCA 1 cut(s) 1502
MspA1I CMGCKG 1 cut(s) 508
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 1 cut(s) 701
MspR9I CCNGG 3 cut(s) 701, 1366, 1447
MssI GTTTAAAC 1 cut(s) 1194
MunI CAATTG 2 cut(s) 786, 1507
MvaI CCWGG 2 cut(s) 1366, 1447
MwoI GCNNNNNNNGC 2 cut(s) 951, 1406
NciI CCSGG 1 cut(s) 701
NdeI CATATG 1 cut(s) 371
NdeII GATC 4 cut(s) 366, 696, 1151, 1468
NlaIII CATG 8 cut(s) 98, 109, 242, 380, 459, 1188, 1257, 1293
NlaIV GGNNCC 2 cut(s) 698, 1628
NmuCI GTSAC 2 cut(s) 553, 1158
NspI RCATGY 4 cut(s) 109, 242, 380, 1257
NspV TTCGAA 1 cut(s) 517
PciI ACATGT 3 cut(s) 105, 238, 1253
PfeI GAWTC 5 cut(s) 314, 526, 588, 593, 1357
PflMI CCANNNNNTGG 1 cut(s) 1509
PfoI TCCNGGA 1 cut(s) 699
PkrI GCNGC 3 cut(s) 773, 793, 1262
PleI GAGTC 3 cut(s) 289, 957, 1124
PmeI GTTTAAAC 1 cut(s) 1194
PpsI GAGTC 3 cut(s) 289, 957, 1124
PscI ACATGT 3 cut(s) 105, 238, 1253
PshAI GACNNNNGTC 1 cut(s) 1323
PsiI TTATAA 2 cut(s) 668, 1236
Psp6I CCWGG 2 cut(s) 1364, 1445
PspEI GGTNACC 1 cut(s) 1121
PspGI CCWGG 2 cut(s) 1364, 1445
PspN4I GGNNCC 2 cut(s) 698, 1628
PspPI GGNCC 1 cut(s) 498
PsuI RGATCY 2 cut(s) 696, 1151
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 6 cut(s) 680, 908, 933, 1006, 1016, 1490
RsaNI GTAC 6 cut(s) 679, 907, 932, 1005, 1015, 1489
RseI CAYNNNNRTG 2 cut(s) 443, 1496
SaqAI TTAA 4 cut(s) 644, 900, 1038, 1193
SatI GCNGC 3 cut(s) 772, 792, 1261
Sau3AI GATC 4 cut(s) 366, 696, 1151, 1468
Sau96I GGNCC 1 cut(s) 498
SchI GAGTC 3 cut(s) 289, 957, 1124
ScrFI CCNGG 3 cut(s) 701, 1366, 1447
SduI GDGCHC 1 cut(s) 138
SfaNI GCATC 2 cut(s) 250, 266
SfuI TTCGAA 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 443, 1496
SmlI CTYRAG 2 cut(s) 890, 1037
SmoI CTYRAG 2 cut(s) 890, 1037
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 8 cut(s) 85, 195, 685, 786, 1044, 1097, 1330, 1507
SspI AATATT 1 cut(s) 1147
SspMI CTAG 3 cut(s) 63, 632, 1008
StyD4I CCNGG 3 cut(s) 699, 1364, 1445
StyI CCWWGG 3 cut(s) 172, 940, 1026
TaaI ACNGT 3 cut(s) 228, 767, 1338
TaiI ACGT 1 cut(s) 1202
TaqI TCGA 2 cut(s) 517, 591
TasI AATT 8 cut(s) 85, 195, 685, 786, 1044, 1097, 1330, 1507
TatI WGTACW 4 cut(s) 678, 931, 1004, 1488
TfiI GAWTC 5 cut(s) 314, 526, 588, 593, 1357
Tru1I TTAA 4 cut(s) 644, 900, 1038, 1193
Tru9I TTAA 4 cut(s) 644, 900, 1038, 1193
TscAI CASTG 1 cut(s) 608
TseFI GTSAC 2 cut(s) 553, 1158
TseI GCWGC 3 cut(s) 771, 791, 1260
Tsp45I GTSAC 2 cut(s) 553, 1158
TspGWI ACGGA 2 cut(s) 732, 838
TspRI CASTG 1 cut(s) 608
Van91I CCANNNNNTGG 1 cut(s) 1509
Vha464I CTTAAG 1 cut(s) 1037
XapI RAATTY 1 cut(s) 1330
XceI RCATGY 4 cut(s) 109, 242, 380, 1257
XcmI CCANNNNNNNNNTGG 3 cut(s) 1111, 1335, 1603
XmiI GTMKAC 1 cut(s) 254
XspI CTAG 3 cut(s) 63, 632, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.