pycom14g03520

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Reverse (-)
2975462 .. 2976732
1271 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 897 bp
ATGTCGTATATTCGTACGGATTTTGAGCCCCCATCGTTTAAAGCCTTGGGCCTTTACAAACAGTTCTTTGCTTTTCAGGCCCAGTTTCAATCTCTGTTTTCTCCAACAACTGGACCCACATCTCCAACTCCGCTTCCTTTCCAGCTTAACAAAAAACCCATTTTCTCTGTCCTCCAAGAATCCCCAGTCGCCGGATCAACCATGGCGGTGATGTCTCCATGGACGACGACCTTGATGATCACGATTATGATGATAACGACGGTCATGCATGTAATCGACGGCAGCGACAACAACCCCGTGTACTCGCCGTGCGCGGACACGAAGGTGGAGAGGTCCGACGGGTTCACCTTCGGAATTGCCTTCGCCTCCAAGGAATCCTTCCAAGTAAACGGCACCGTTCAGCTGTCTCCTTGCGACTCTAGGCTCTCTCTCTCCTCCAATGCCCAGATCTCCGTTTTCCGACCCAAAGTTGACGATCGATCTCCCTCCTCTCCGTCAACTCCTCCTCCTTCAATCCGGATACTTATGGGTTTATGGTTGCGTTTGCCGGGCGAAAATATGCTGCAAGGTCCCTTCCTACTTTTGTTGCTAATGGTACCTACACTGTCACCAGCTTTACCCTTGTGCTTGAGTTCAACAAGGGCAGGCTGCAGAACTTGTACTGGAAAAAGGATGGGTGCTCTAAATGTTCAGGGAGCTCCGACTTTGTTTGCCTCAACAATCAGCACTGCGCCTTCAAGACCTCGAGCTGCAAAAACCATGGCGGCTCTGTGGACTGCAGCCTCGGAATCCAGTTGGCATTTTCCGGCACAGACAAGCACCTTTCGGTTCTTAACTCATGGTATGAAGTGGCAAACCTCAGGCAGTACTCGCTCTATGGTCTTTATTCGAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

32.5

Weight (kDa)

9.86

Isoelectric Point (pI)

60.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 595
AccB1I GGYRCC 2 cut(s) 392, 595
AccB7I CCANNNNNTGG 1 cut(s) 110
AccII CGCG 1 cut(s) 314
AccIII TCCGGA 1 cut(s) 516
AciI CCGC 4 cut(s) 131, 206, 314, 764
AclWI GGATC 1 cut(s) 202
AcsI RAATTY 1 cut(s) 891
AfaI GTAC 5 cut(s) 16, 302, 597, 661, 868
AfiI CCNNNNNNNGG 2 cut(s) 110, 191
AgsI TTSAA 4 cut(s) 89, 513, 636, 738
AjuI GAANNNNNNNTTGG 2 cut(s) 362, 394
AleI CACNNNNGTG 1 cut(s) 323
AluBI AGCT 5 cut(s) 145, 403, 614, 698, 749
AluI AGCT 5 cut(s) 145, 403, 614, 698, 749
Alw21I GWGCWC 2 cut(s) 682, 700
Alw26I GTCTC 2 cut(s) 219, 411
AlwI GGATC 1 cut(s) 202
Ama87I CYCGRG 1 cut(s) 744
Aor13HI TCCGGA 1 cut(s) 516
AoxI GGCC 2 cut(s) 49, 78
ApeKI GCWGC 5 cut(s) 282, 562, 648, 749, 779
ApoI RAATTY 1 cut(s) 891
Asp718I GGTACC 1 cut(s) 595
AspLEI GCGC 2 cut(s) 314, 733
AspS9I GGNCC 5 cut(s) 49, 79, 113, 333, 569
AsuC2I CCSGG 1 cut(s) 549
AsuHPI GGTGA 3 cut(s) 220, 337, 600
AsuII TTCGAA 1 cut(s) 889
AvaI CYCGRG 1 cut(s) 744
AvaII GGWCC 3 cut(s) 113, 333, 569
AxyI CCTNAGG 1 cut(s) 859
BanI GGYRCC 2 cut(s) 392, 595
BanII GRGCYC 2 cut(s) 30, 700
Bbv12I GWGCWC 2 cut(s) 682, 700
BbvI GCAGC 5 cut(s) 294, 549, 635, 736, 791
BccI CCATC 2 cut(s) 40, 667
BceAI ACGGC 3 cut(s) 292, 295, 406
BcgI CGANNNNNNTGC 2 cut(s) 247, 281
BciVI GTATCC 1 cut(s) 513
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 1 cut(s) 549
BcoDI GTCTC 2 cut(s) 219, 411
BfaI CTAG 1 cut(s) 420
BfmI CTRYAG 2 cut(s) 649, 777
BfuI GTATCC 1 cut(s) 513
BglII AGATCT 1 cut(s) 447
BisI GCNGC 6 cut(s) 283, 563, 649, 750, 765, 780
BlsI GCNGC 6 cut(s) 284, 564, 650, 751, 766, 781
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 1 cut(s) 549
Bme18I GGWCC 3 cut(s) 113, 333, 569
BmeT110I CYCGRG 1 cut(s) 744
BmgT120I GGNCC 5 cut(s) 49, 79, 113, 333, 569
BmiI GGNNCC 4 cut(s) 115, 394, 571, 597
BmrFI CCNGG 1 cut(s) 549
BmrI ACTGGG 2 cut(s) 76, 179
BmuI ACTGGG 2 cut(s) 76, 179
Bpu14I TTCGAA 1 cut(s) 889
BpuEI CTTGAG 1 cut(s) 649
BpuMI CCSGG 1 cut(s) 549
Bsa29I ATCGAT 1 cut(s) 478
BsaJI CCNNGG 6 cut(s) 45, 201, 218, 369, 759, 783
BsaWI WCCGGW 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 490, 520
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 191
Bse1I ACTGG 5 cut(s) 82, 115, 185, 667, 792
Bse21I CCTNAGG 1 cut(s) 859
BseAI TCCGGA 1 cut(s) 516
BseCI ATCGAT 1 cut(s) 478
BseDI CCNNGG 6 cut(s) 45, 201, 218, 369, 759, 783
BseGI GGATG 1 cut(s) 678
BseLI CCNNNNNNNGG 2 cut(s) 110, 191
BseMII CTCAG 1 cut(s) 873
BseNI ACTGG 5 cut(s) 82, 115, 185, 667, 792
BseRI GAGGAG 4 cut(s) 424, 478, 492, 495
BseXI GCAGC 5 cut(s) 294, 549, 635, 736, 791
Bsh1236I CGCG 1 cut(s) 314
Bsh1285I CGRYCG 1 cut(s) 478
BshFI GGCC 2 cut(s) 51, 80
BshNI GGYRCC 2 cut(s) 392, 595
BshVI ATCGAT 1 cut(s) 478
BsiEI CGRYCG 1 cut(s) 478
BsiHKAI GWGCWC 2 cut(s) 682, 700
BsiHKCI CYCGRG 1 cut(s) 744
BsiSI CCGG 4 cut(s) 192, 517, 548, 806
BsiWI CGTACG 1 cut(s) 14
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 2 cut(s) 110, 191
BsmAI GTCTC 2 cut(s) 219, 411
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 2 cut(s) 51, 80
BsoBI CYCGRG 1 cut(s) 744
Bsp119I TTCGAA 1 cut(s) 889
Bsp1286I GDGCHC 3 cut(s) 30, 682, 700
Bsp13I TCCGGA 1 cut(s) 516
Bsp143I GATC 5 cut(s) 194, 237, 447, 475, 479
Bsp19I CCATGG 3 cut(s) 201, 218, 759
BspACI CCGC 4 cut(s) 131, 206, 314, 764
BspANI GGCC 2 cut(s) 51, 80
BspCNI CTCAG 1 cut(s) 872
BspDI ATCGAT 1 cut(s) 478
BspEI TCCGGA 1 cut(s) 516
BspFNI CGCG 1 cut(s) 314
BspLI GGNNCC 4 cut(s) 115, 394, 571, 597
BspMAI CTGCAG 2 cut(s) 653, 781
BspPI GGATC 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 889
BspT107I GGYRCC 2 cut(s) 392, 595
BsrI ACTGG 5 cut(s) 82, 115, 185, 667, 792
BssECI CCNNGG 6 cut(s) 45, 201, 218, 369, 759, 783
BssMI GATC 5 cut(s) 194, 237, 447, 475, 479
BssT1I CCWWGG 5 cut(s) 45, 201, 218, 369, 759
Bst4CI ACNGT 4 cut(s) 63, 262, 397, 606
BstBI TTCGAA 1 cut(s) 889
BstC8I GCNNGC 1 cut(s) 646
BstDEI CTNAG 1 cut(s) 859
BstDSI CCRYGG 3 cut(s) 201, 218, 759
BstF5I GGATG 1 cut(s) 678
BstFNI CGCG 1 cut(s) 314
BstHHI GCGC 2 cut(s) 314, 733
BstKTI GATC 5 cut(s) 197, 240, 450, 478, 482
BstMAI GTCTC 2 cut(s) 219, 411
BstMBI GATC 5 cut(s) 194, 237, 447, 475, 479
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 2 cut(s) 77, 870
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 1 cut(s) 547
BstSFI CTRYAG 2 cut(s) 649, 777
BstUI CGCG 1 cut(s) 314
BstV1I GCAGC 5 cut(s) 294, 549, 635, 736, 791
BstX2I RGATCY 1 cut(s) 447
BstYI RGATCY 1 cut(s) 447
Bsu15I ATCGAT 1 cut(s) 478
Bsu36I CCTNAGG 1 cut(s) 859
BsuI GTATCC 1 cut(s) 513
BsuRI GGCC 2 cut(s) 51, 80
BsuTUI ATCGAT 1 cut(s) 478
BtgI CCRYGG 3 cut(s) 201, 218, 759
BtsCI GGATG 1 cut(s) 678
BtsI GCAGTG 1 cut(s) 726
BtsIMutI CAGTG 2 cut(s) 602, 726
Cac8I GCNNGC 1 cut(s) 646
CfoI GCGC 2 cut(s) 314, 733
Cfr13I GGNCC 5 cut(s) 49, 79, 113, 333, 569
ClaI ATCGAT 1 cut(s) 478
Csp6I GTAC 5 cut(s) 15, 301, 596, 660, 867
CviAII CATG 6 cut(s) 202, 219, 265, 269, 760, 839
CviQI GTAC 5 cut(s) 15, 301, 596, 660, 867
DdeI CTNAG 1 cut(s) 859
DpnI GATC 5 cut(s) 196, 239, 449, 477, 481
DpnII GATC 5 cut(s) 194, 237, 447, 475, 479
DraI TTTAAA 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 698
Eco130I CCWWGG 5 cut(s) 45, 201, 218, 369, 759
Eco24I GRGCYC 2 cut(s) 30, 700
Eco47I GGWCC 3 cut(s) 113, 333, 569
Eco53kI GAGCTC 1 cut(s) 698
Eco81I CCTNAGG 1 cut(s) 859
Eco88I CYCGRG 1 cut(s) 744
EcoICRI GAGCTC 1 cut(s) 698
EcoO109I RGGNCCY 1 cut(s) 569
EcoT14I CCWWGG 5 cut(s) 45, 201, 218, 369, 759
EcoT22I ATGCAT 1 cut(s) 270
EcoT38I GRGCYC 2 cut(s) 30, 700
ErhI CCWWGG 5 cut(s) 45, 201, 218, 369, 759
FaeI CATG 6 cut(s) 205, 222, 268, 272, 763, 842
FalI AAGNNNNNCTT 2 cut(s) 362, 394
FaqI GGGAC 1 cut(s) 555
FatI CATG 6 cut(s) 201, 218, 264, 268, 759, 838
FbaI TGATCA 1 cut(s) 237
Fnu4HI GCNGC 6 cut(s) 283, 563, 649, 750, 765, 780
FokI GGATG 1 cut(s) 685
FriOI GRGCYC 2 cut(s) 30, 700
Fsp4HI GCNGC 6 cut(s) 283, 563, 649, 750, 765, 780
FspBI CTAG 1 cut(s) 420
GlaI GCGC 2 cut(s) 313, 732
GluI GCNGC 6 cut(s) 283, 563, 649, 750, 765, 780
HaeIII GGCC 2 cut(s) 51, 80
HapII CCGG 4 cut(s) 192, 517, 548, 806
HhaI GCGC 2 cut(s) 314, 733
Hin1II CATG 6 cut(s) 205, 222, 268, 272, 763, 842
Hin6I GCGC 2 cut(s) 312, 731
HinP1I GCGC 2 cut(s) 312, 731
HincII GTYRAC 2 cut(s) 472, 498
HindII GTYRAC 2 cut(s) 472, 498
HinfI GANTC 4 cut(s) 179, 374, 416, 788
HpaII CCGG 4 cut(s) 192, 517, 548, 806
HphI GGTGA 3 cut(s) 220, 337, 600
Hpy166II GTNNAC 6 cut(s) 301, 345, 388, 472, 498, 774
Hpy188I TCNGA 5 cut(s) 337, 353, 461, 702, 787
Hpy188III TCNNGA 3 cut(s) 241, 517, 738
Hpy8I GTNNAC 6 cut(s) 301, 345, 388, 472, 498, 774
Hpy99I CGWCG 4 cut(s) 229, 262, 281, 341
HpyAV CCTTC 7 cut(s) 316, 358, 370, 388, 519, 583, 744
HpyCH4III ACNGT 4 cut(s) 63, 262, 397, 606
HpyCH4V TGCA 5 cut(s) 268, 565, 651, 752, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 870
HpyF3I CTNAG 1 cut(s) 859
Hsp92II CATG 6 cut(s) 205, 222, 268, 272, 763, 842
HspAI GCGC 2 cut(s) 312, 731
Kpn2I TCCGGA 1 cut(s) 516
KpnI GGTACC 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 5 cut(s) 194, 237, 447, 475, 479
LmnI GCTCC 2 cut(s) 695, 703
Lsp1109I GCAGC 5 cut(s) 294, 549, 635, 736, 791
MaeI CTAG 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 606
MalI GATC 5 cut(s) 196, 239, 449, 477, 481
MboI GATC 5 cut(s) 194, 237, 447, 475, 479
MflI RGATCY 1 cut(s) 447
MhlI GDGCHC 3 cut(s) 30, 682, 700
MluCI AATT 2 cut(s) 354, 891
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 5 cut(s) 128, 149, 360, 484, 725
Mph1103I ATGCAT 1 cut(s) 270
MroI TCCGGA 1 cut(s) 516
MseI TTAA 3 cut(s) 39, 147, 833
MslI CAYNNNNRTG 3 cut(s) 206, 245, 323
MspA1I CMGCKG 1 cut(s) 403
MspI CCGG 4 cut(s) 192, 517, 548, 806
MspR9I CCNGG 1 cut(s) 549
MvnI CGCG 1 cut(s) 314
MwoI GCNNNNNNNGC 2 cut(s) 77, 870
NciI CCSGG 1 cut(s) 549
NcoI CCATGG 3 cut(s) 201, 218, 759
NdeII GATC 5 cut(s) 194, 237, 447, 475, 479
NlaIII CATG 6 cut(s) 205, 222, 268, 272, 763, 842
NlaIV GGNNCC 4 cut(s) 115, 394, 571, 597
NmuCI GTSAC 1 cut(s) 606
NsiI ATGCAT 1 cut(s) 270
NspI RCATGY 1 cut(s) 272
NspV TTCGAA 1 cut(s) 889
OliI CACNNNNGTG 1 cut(s) 323
PaeR7I CTCGAG 1 cut(s) 744
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PfeI GAWTC 3 cut(s) 179, 374, 788
Pfl23II CGTACG 1 cut(s) 14
PflMI CCANNNNNTGG 1 cut(s) 110
PkrI GCNGC 6 cut(s) 284, 564, 650, 751, 766, 781
Ple19I CGATCG 1 cut(s) 478
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
PpuMI RGGWCCY 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 700
Psp5II RGGWCCY 1 cut(s) 569
PspLI CGTACG 1 cut(s) 14
PspN4I GGNNCC 4 cut(s) 115, 394, 571, 597
PspPI GGNCC 5 cut(s) 49, 79, 113, 333, 569
PspPPI RGGWCCY 1 cut(s) 569
PspXI VCTCGAGB 1 cut(s) 744
PstI CTGCAG 2 cut(s) 653, 781
PsuI RGATCY 1 cut(s) 447
PvuI CGATCG 1 cut(s) 478
PvuII CAGCTG 1 cut(s) 403
RsaI GTAC 5 cut(s) 16, 302, 597, 661, 868
RsaNI GTAC 5 cut(s) 15, 301, 596, 660, 867
RseI CAYNNNNRTG 3 cut(s) 206, 245, 323
SacI GAGCTC 1 cut(s) 700
SaqAI TTAA 3 cut(s) 39, 147, 833
SatI GCNGC 6 cut(s) 283, 563, 649, 750, 765, 780
Sau3AI GATC 5 cut(s) 194, 237, 447, 475, 479
Sau96I GGNCC 5 cut(s) 49, 79, 113, 333, 569
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 1 cut(s) 549
SduI GDGCHC 3 cut(s) 30, 682, 700
SfcI CTRYAG 2 cut(s) 649, 777
Sfr274I CTCGAG 1 cut(s) 744
SfuI TTCGAA 1 cut(s) 889
SinI GGWCC 3 cut(s) 113, 333, 569
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 3 cut(s) 206, 245, 323
SmlI CTYRAG 2 cut(s) 628, 744
SmoI CTYRAG 2 cut(s) 628, 744
Sse9I AATT 2 cut(s) 354, 891
SsiI CCGC 4 cut(s) 131, 206, 314, 764
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 700
StyD4I CCNGG 1 cut(s) 547
StyI CCWWGG 5 cut(s) 45, 201, 218, 369, 759
TaaI ACNGT 4 cut(s) 63, 262, 397, 606
TaqI TCGA 4 cut(s) 276, 478, 745, 889
TasI AATT 2 cut(s) 354, 891
TatI WGTACW 3 cut(s) 300, 659, 866
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 3 cut(s) 179, 374, 788
Tru1I TTAA 3 cut(s) 39, 147, 833
Tru9I TTAA 3 cut(s) 39, 147, 833
TscAI CASTG 2 cut(s) 609, 733
TseFI GTSAC 1 cut(s) 606
TseI GCWGC 5 cut(s) 282, 562, 648, 749, 779
Tsp45I GTSAC 1 cut(s) 606
TspDTI ATGAA 1 cut(s) 860
TspGWI ACGGA 3 cut(s) 32, 442, 483
TspRI CASTG 2 cut(s) 609, 733
Van91I CCANNNNNTGG 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 113, 333, 569
XapI RAATTY 1 cut(s) 891
XceI RCATGY 1 cut(s) 272
XhoI CTCGAG 1 cut(s) 744
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.