pycom14g03930

Pentatricopeptide repeat-containing protein At2g20710, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Reverse (-)
3411930 .. 3412861
932 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGCAGAAGATGAGGGATTTGGGGTTTGCTAGGACACCGTTGTCCTACAACATTTTGCTCAATTTGGCATTGGTCGTGACAAATACACTTATGGTATCCAGCTCAATGCATGTGCAGCTGCTTCTGATCTTGAGGGAATTGACAAGCTTATGGCAGAGTGGGATTTTGGATTGGGATAGTAATGACAATGCAGAAAATGGTTATACGAAAGCACGGAATGTGAAAAAGGCATTGGCAATGCTAGAGAAGGTTGAGAAACTGATACCAAGTTCTGAAAGACAGAGGATTATGAGTACCTTATCAGACAATCTCTATGATGAATGGGAATCCAAGAATTTTACTCCAGATATTCGTATCACAAATTTCTTGATCAGTGCTTATACCAGAAATGGCCTAGTTGACAAGGCTGAATCCATTATTAACAGTGTAATACTAAAGGGTGGGAAGCCGGATGCAAGCACTTGGTTTTATCTGGCAAGGGGCTATCTCGATCAGGATCAAGCTGAGAAGACAGTTGAGTCGATGAAGAAAGCACTTTCAGTTTCGGGGCCTCCGTTTTGGCTAGACAGGGATGTTTTGGCTGCTTGTATGGGATACATGGGACAGAAATATGATGTGGAGGGAGCTCGAGAATATATAAGACTACTCGTGGGAGTTGAAGAATTTTTAAGACTACTCGAGGAAACTCCCGAAATTGTGGGTAGCAAATGCAAATCAACTTCAGCGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

244

Amino Acids

27.43

Weight (kDa)

5.65

Isoelectric Point (pI)

35.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 40, 517
AclWI GGATC 1 cut(s) 504
AcsI RAATTY 3 cut(s) 334, 361, 662
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 1 cut(s) 295
AgsI TTSAA 1 cut(s) 659
AjuI GAANNNNNNNTTGG 2 cut(s) 215, 247
AluBI AGCT 5 cut(s) 102, 118, 147, 503, 626
AluI AGCT 5 cut(s) 102, 118, 147, 503, 626
Alw21I GWGCWC 1 cut(s) 628
AlwI GGATC 1 cut(s) 504
Ama87I CYCGRG 2 cut(s) 627, 677
AoxI GGCC 2 cut(s) 391, 548
ApeKI GCWGC 3 cut(s) 115, 118, 581
ApoI RAATTY 3 cut(s) 334, 361, 662
AspS9I GGNCC 1 cut(s) 548
AvaI CYCGRG 2 cut(s) 627, 677
BanII GRGCYC 1 cut(s) 628
BauI CACGAG 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 3 cut(s) 105, 127, 568
BciVI GTATCC 2 cut(s) 106, 587
BclI TGATCA 1 cut(s) 369
BfaI CTAG 4 cut(s) 30, 242, 395, 563
BfuI GTATCC 2 cut(s) 106, 587
BisI GCNGC 3 cut(s) 116, 119, 582
BlsI GCNGC 3 cut(s) 117, 120, 583
BmeT110I CYCGRG 2 cut(s) 627, 677
BmgT120I GGNCC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 549
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 327
BpuEI CTTGAG 1 cut(s) 151
BsaBI GATNNNNATC 1 cut(s) 495
Bse3DI GCAATG 1 cut(s) 243
Bse8I GATNNNNATC 1 cut(s) 495
BseGI GGATG 2 cut(s) 457, 577
BseJI GATNNNNATC 1 cut(s) 495
BseMI GCAATG 1 cut(s) 243
BseMII CTCAG 1 cut(s) 495
BseXI GCAGC 3 cut(s) 105, 127, 568
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 2 cut(s) 393, 550
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 2 cut(s) 627, 677
BsiSI CCGG 1 cut(s) 449
BslFI GGGAC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 615
BsnI GGCC 2 cut(s) 393, 550
BsoBI CYCGRG 2 cut(s) 627, 677
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 4 cut(s) 126, 369, 490, 496
BspANI GGCC 2 cut(s) 393, 550
BspCNI CTCAG 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 549
BspPI GGATC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 243
BssMI GATC 4 cut(s) 126, 369, 490, 496
BssSI CACGAG 1 cut(s) 647
Bst2BI CACGAG 1 cut(s) 647
Bst4CI ACNGT 3 cut(s) 39, 425, 514
BstC8I GCNNGC 1 cut(s) 457
BstDEI CTNAG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 457, 577
BstKTI GATC 4 cut(s) 129, 372, 493, 499
BstMBI GATC 4 cut(s) 126, 369, 490, 496
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNSI RCATGY 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 105, 127, 568
BstV2I GAAGAC 1 cut(s) 515
BsuI GTATCC 2 cut(s) 106, 587
BsuRI GGCC 2 cut(s) 393, 550
BtsCI GGATG 2 cut(s) 457, 577
BtsIMutI CAGTG 2 cut(s) 379, 430
Cac8I GCNNGC 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 1 cut(s) 294
CviAII CATG 2 cut(s) 110, 598
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 1 cut(s) 504
DpnI GATC 4 cut(s) 128, 371, 492, 498
DpnII GATC 4 cut(s) 126, 369, 490, 496
DrdI GACNNNNNNGTC 2 cut(s) 40, 517
DseDI GACNNNNNNGTC 2 cut(s) 40, 517
Ecl136II GAGCTC 1 cut(s) 626
Eco24I GRGCYC 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 626
Eco57I CTGAAG 1 cut(s) 705
Eco88I CYCGRG 2 cut(s) 627, 677
EcoICRI GAGCTC 1 cut(s) 626
EcoO109I RGGNCCY 1 cut(s) 548
EcoT22I ATGCAT 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 628
FaeI CATG 2 cut(s) 113, 601
FaqI GGGAC 1 cut(s) 615
FatI CATG 2 cut(s) 109, 597
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 116, 119, 582
FokI GGATG 2 cut(s) 464, 584
FriOI GRGCYC 1 cut(s) 628
Fsp4HI GCNGC 3 cut(s) 116, 119, 582
FspBI CTAG 4 cut(s) 30, 242, 395, 563
GluI GCNGC 3 cut(s) 116, 119, 582
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 2 cut(s) 393, 550
HapII CCGG 1 cut(s) 449
Hin1II CATG 2 cut(s) 113, 601
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 3 cut(s) 326, 410, 518
HpaII CCGG 1 cut(s) 449
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 3 cut(s) 126, 274, 304
Hpy188III TCNNGA 8 cut(s) 76, 130, 344, 367, 488, 494, 629, 689
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 3 cut(s) 39, 425, 514
HpyCH4V TGCA 6 cut(s) 4, 109, 115, 191, 455, 711
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpyF3I CTNAG 1 cut(s) 504
Hsp92II CATG 2 cut(s) 113, 601
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 4 cut(s) 126, 369, 490, 496
LmnI GCTCC 1 cut(s) 623
LpnPI CCDG 7 cut(s) 112, 357, 397, 458, 462, 479, 553
Lsp1109I GCAGC 3 cut(s) 105, 127, 568
LweI GCATC 1 cut(s) 442
MaeI CTAG 4 cut(s) 30, 242, 395, 563
MaeIII GTNAC 1 cut(s) 76
MalI GATC 4 cut(s) 128, 371, 492, 498
MboI GATC 4 cut(s) 126, 369, 490, 496
MboII GAAGA 4 cut(s) 19, 520, 538, 671
MhlI GDGCHC 1 cut(s) 628
MluCI AATT 6 cut(s) 61, 137, 334, 361, 662, 693
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 6 cut(s) 6, 126, 276, 561, 613, 673
Mph1103I ATGCAT 1 cut(s) 111
MseI TTAA 3 cut(s) 420, 668, 730
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeII GATC 4 cut(s) 126, 369, 490, 496
NlaIII CATG 2 cut(s) 113, 601
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 1 cut(s) 76
NsiI ATGCAT 1 cut(s) 111
NspI RCATGY 1 cut(s) 113
PaeR7I CTCGAG 2 cut(s) 627, 677
PcsI WCGNNNNNNNCGW 1 cut(s) 551
PfeI GAWTC 2 cut(s) 326, 410
PkrI GCNGC 3 cut(s) 117, 120, 583
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp124BI GAGCTC 1 cut(s) 628
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 118
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
SacI GAGCTC 1 cut(s) 628
SaqAI TTAA 3 cut(s) 420, 668, 730
SatI GCNGC 3 cut(s) 116, 119, 582
Sau3AI GATC 4 cut(s) 126, 369, 490, 496
Sau96I GGNCC 1 cut(s) 548
SchI GAGTC 1 cut(s) 527
SduI GDGCHC 1 cut(s) 628
SetI ASST 7 cut(s) 104, 120, 149, 252, 299, 505, 628
SfaNI GCATC 1 cut(s) 442
Sfr274I CTCGAG 2 cut(s) 627, 677
SlaI CTCGAG 2 cut(s) 627, 677
SmlI CTYRAG 3 cut(s) 130, 627, 677
SmoI CTYRAG 3 cut(s) 130, 627, 677
Sse9I AATT 6 cut(s) 61, 137, 334, 361, 662, 693
SspMI CTAG 4 cut(s) 30, 242, 395, 563
SstI GAGCTC 1 cut(s) 628
TaaI ACNGT 3 cut(s) 39, 425, 514
TaqI TCGA 4 cut(s) 489, 521, 628, 678
TasI AATT 6 cut(s) 61, 137, 334, 361, 662, 693
TfiI GAWTC 2 cut(s) 326, 410
Tru1I TTAA 3 cut(s) 420, 668, 730
Tru9I TTAA 3 cut(s) 420, 668, 730
TscAI CASTG 2 cut(s) 379, 430
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 3 cut(s) 115, 118, 581
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 333, 539
TspGWI ACGGA 2 cut(s) 229, 543
TspRI CASTG 2 cut(s) 379, 430
XapI RAATTY 3 cut(s) 334, 361, 662
XceI RCATGY 1 cut(s) 113
XhoI CTCGAG 2 cut(s) 627, 677
XspI CTAG 4 cut(s) 30, 242, 395, 563
Zsp2I ATGCAT 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.