pycom14g05210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
5003389 .. 5004021
633 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 585 bp
ATGCCAAACACCCGAGTCCGTATAACTAATCCTATCCGATCCTTTATCCGATCTGCCAAACTGGGCCAAATAAATAAAACCCAAGGAGATAAATGTGACGAAGGCCCATCAAATAAGCAAGTACCACAGAAAATCAAAAGTTCACTCTCCACAAAGCTGCTCTCTCTCTTCCTCTCTCTCTCCCTTACGAGCTGGGAGCGAAAGTCTTCACCATACCCGCCTCCACAATGCTCCGCCATCGCCTCGTCCAGGCGCTCCGAACCCGCGGCGGCTCTGCCACCGGTCCGAGCCGGTGGACCAGCCCGGGCCACGAGGAACAACCCAAAGGCTACCTCTTCAATCGGACCCCACCTCCCCCAGGCCAATCTCGGAAATGGGAGGATTGGGAGCTCCCGTGCTACGTCACCAGCTTCCTCACCATCGTCATCCTCGGCGTTGGCCTCAACGCCAAGCCTGATCTCACCATCGAGACTTGGGCTCACCAGAAAGCCCTCGAACGGCTCGAGATCGATCAGGCCGGCCTCTCTGTCGCCGGGTCGGGCGAATCTGACTGAAATGCGATCTGGGTATGCCGGTTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

20.34

Weight (kDa)

11.82

Isoelectric Point (pI)

73.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 266
AciI CCGC 5 cut(s) 218, 234, 264, 266, 269
AclWI GGATC 1 cut(s) 33
AfaI GTAC 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 249, 358, 497
AgeI ACCGGT 1 cut(s) 280
AgsI TTSAA 1 cut(s) 339
AjnI CCWGG 2 cut(s) 248, 357
AluBI AGCT 4 cut(s) 157, 192, 390, 410
AluI AGCT 4 cut(s) 157, 192, 390, 410
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 463
AlwI GGATC 1 cut(s) 33
Ama87I CYCGRG 3 cut(s) 12, 303, 502
AoxI GGCC 7 cut(s) 64, 103, 306, 360, 438, 515, 519
ApeKI GCWGC 1 cut(s) 157
AsiGI ACCGGT 1 cut(s) 280
Asp700I GAANNNNTTC 1 cut(s) 205
AspLEI GCGC 1 cut(s) 255
AspS9I GGNCC 6 cut(s) 64, 104, 283, 296, 306, 344
AsuC2I CCSGG 3 cut(s) 304, 305, 534
AsuHPI GGTGA 5 cut(s) 201, 396, 408, 453, 472
AvaI CYCGRG 3 cut(s) 12, 303, 502
AvaII GGWCC 3 cut(s) 283, 296, 344
BanII GRGCYC 2 cut(s) 392, 480
BauI CACGAG 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 1 cut(s) 144
BccI CCATC 4 cut(s) 115, 245, 427, 472
BceAI ACGGC 1 cut(s) 514
BciT130I CCWGG 2 cut(s) 250, 359
BcnI CCSGG 3 cut(s) 304, 305, 534
BcoDI GTCTC 1 cut(s) 463
BfoI RGCGCY 1 cut(s) 256
BisI GCNGC 3 cut(s) 158, 267, 270
BlsI GCNGC 3 cut(s) 159, 268, 271
Bme1390I CCNGG 5 cut(s) 250, 304, 305, 359, 534
Bme18I GGWCC 3 cut(s) 283, 296, 344
BmeT110I CYCGRG 3 cut(s) 12, 303, 502
BmgT120I GGNCC 6 cut(s) 64, 104, 283, 296, 306, 344
BmiI GGNNCC 1 cut(s) 346
BmrFI CCNGG 5 cut(s) 250, 304, 305, 359, 534
BmrI ACTGGG 1 cut(s) 71
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 198
BpuMI CCSGG 3 cut(s) 304, 305, 534
Bsa29I ATCGAT 1 cut(s) 509
BsaJI CCNNGG 5 cut(s) 82, 264, 303, 357, 429
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 6 cut(s) 188, 218, 336, 366, 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 249, 358, 497
Bse118I RCCGGY 4 cut(s) 280, 290, 517, 572
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 2 cut(s) 250, 359
BseCI ATCGAT 1 cut(s) 509
BseDI CCNNGG 5 cut(s) 82, 264, 303, 357, 429
BseGI GGATG 1 cut(s) 425
BseLI CCNNNNNNNGG 3 cut(s) 249, 358, 497
BseNI ACTGG 1 cut(s) 66
BseXI GCAGC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 7 cut(s) 66, 105, 308, 362, 440, 517, 521
BshTI ACCGGT 1 cut(s) 280
BshVI ATCGAT 1 cut(s) 509
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 3 cut(s) 12, 303, 502
BsiSI CCGG 6 cut(s) 281, 291, 304, 518, 533, 573
BslI CCNNNNNNNGG 3 cut(s) 249, 358, 497
BsmAI GTCTC 1 cut(s) 463
BsnI GGCC 7 cut(s) 66, 105, 308, 362, 440, 517, 521
BsoBI CYCGRG 3 cut(s) 12, 303, 502
Bsp1286I GDGCHC 2 cut(s) 392, 480
Bsp143I GATC 6 cut(s) 38, 50, 456, 506, 510, 560
BspACI CCGC 5 cut(s) 218, 234, 264, 266, 269
BspANI GGCC 7 cut(s) 66, 105, 308, 362, 440, 517, 521
BspDI ATCGAT 1 cut(s) 509
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 1 cut(s) 346
BspPI GGATC 1 cut(s) 33
BsrFI RCCGGY 4 cut(s) 280, 290, 517, 572
BsrI ACTGG 1 cut(s) 66
BssAI RCCGGY 4 cut(s) 280, 290, 517, 572
BssECI CCNNGG 5 cut(s) 82, 264, 303, 357, 429
BssMI GATC 6 cut(s) 38, 50, 456, 506, 510, 560
BssSI CACGAG 1 cut(s) 310
BssT1I CCWWGG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 310
Bst2UI CCWGG 2 cut(s) 250, 359
Bst6I CTCTTC 2 cut(s) 173, 340
BstC8I GCNNGC 1 cut(s) 519
BstDSI CCRYGG 1 cut(s) 264
BstENI CCTNNNNNAGG 2 cut(s) 247, 356
BstF5I GGATG 1 cut(s) 425
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 1 cut(s) 256
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 6 cut(s) 41, 53, 459, 509, 513, 563
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 6 cut(s) 38, 50, 456, 506, 510, 560
BstNI CCWGG 2 cut(s) 250, 359
BstSCI CCNGG 5 cut(s) 248, 302, 303, 357, 532
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 198
Bsu15I ATCGAT 1 cut(s) 509
BsuRI GGCC 7 cut(s) 66, 105, 308, 362, 440, 517, 521
BsuTUI ATCGAT 1 cut(s) 509
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 1 cut(s) 255
Cfr10I RCCGGY 4 cut(s) 280, 290, 517, 572
Cfr13I GGNCC 6 cut(s) 64, 104, 283, 296, 306, 344
Cfr42I CCGCGG 1 cut(s) 267
Cfr9I CCCGGG 1 cut(s) 303
ClaI ATCGAT 1 cut(s) 509
CpoI CGGWCCG 1 cut(s) 283
Csp6I GTAC 1 cut(s) 122
CspAI ACCGGT 1 cut(s) 280
CspI CGGWCCG 1 cut(s) 283
CviQI GTAC 1 cut(s) 122
DpnI GATC 6 cut(s) 40, 52, 458, 508, 512, 562
DpnII GATC 6 cut(s) 38, 50, 456, 506, 510, 560
Eam1104I CTCTTC 2 cut(s) 173, 340
EarI CTCTTC 2 cut(s) 173, 340
EciI GGCGGA 1 cut(s) 223
Ecl136II GAGCTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 82
Eco24I GRGCYC 2 cut(s) 392, 480
Eco47I GGWCC 3 cut(s) 283, 296, 344
Eco53kI GAGCTC 1 cut(s) 390
Eco88I CYCGRG 3 cut(s) 12, 303, 502
EcoICRI GAGCTC 1 cut(s) 390
EcoNI CCTNNNNNAGG 2 cut(s) 247, 356
EcoRII CCWGG 2 cut(s) 248, 357
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 2 cut(s) 392, 480
ErhI CCWWGG 1 cut(s) 82
FaiI YATR 3 cut(s) 23, 214, 570
FauI CCCGC 2 cut(s) 225, 271
Fnu4HI GCNGC 3 cut(s) 158, 267, 270
FokI GGATG 1 cut(s) 412
FriOI GRGCYC 2 cut(s) 392, 480
FseI GGCCGGCC 1 cut(s) 521
Fsp4HI GCNGC 3 cut(s) 158, 267, 270
GlaI GCGC 1 cut(s) 254
GluI GCNGC 3 cut(s) 158, 267, 270
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 256
HaeIII GGCC 7 cut(s) 66, 105, 308, 362, 440, 517, 521
HapII CCGG 6 cut(s) 281, 291, 304, 518, 533, 573
HhaI GCGC 1 cut(s) 255
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 2 cut(s) 15, 544
HpaII CCGG 6 cut(s) 281, 291, 304, 518, 533, 573
HphI GGTGA 5 cut(s) 201, 396, 408, 453, 472
Hpy166II GTNNAC 2 cut(s) 143, 296
Hpy188I TCNGA 8 cut(s) 38, 50, 259, 287, 344, 371, 549, 584
Hpy188III TCNNGA 2 cut(s) 468, 504
Hpy8I GTNNAC 2 cut(s) 143, 296
HpyAV CCTTC 1 cut(s) 95
HpyCH4IV ACGT 1 cut(s) 401
HpySE526I ACGT 1 cut(s) 401
HspAI GCGC 1 cut(s) 253
KroI GCCGGC 1 cut(s) 517
KroNI GCCGGC 1 cut(s) 519
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 6 cut(s) 38, 50, 456, 506, 510, 560
LmnI GCTCC 5 cut(s) 196, 236, 260, 387, 395
Lsp1109I GCAGC 1 cut(s) 144
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 2 cut(s) 95, 402
MalI GATC 6 cut(s) 40, 52, 458, 508, 512, 562
MboI GATC 6 cut(s) 38, 50, 456, 506, 510, 560
MboII GAAGA 3 cut(s) 160, 198, 327
MhlI GDGCHC 2 cut(s) 392, 480
MlyI GAGTC 1 cut(s) 24
MroNI GCCGGC 1 cut(s) 517
MroXI GAANNNNTTC 1 cut(s) 205
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 6 cut(s) 281, 291, 304, 518, 533, 573
MspR9I CCNGG 5 cut(s) 250, 304, 305, 359, 534
MvaI CCWGG 2 cut(s) 250, 359
MvnI CGCG 1 cut(s) 266
NaeI GCCGGC 1 cut(s) 519
NciI CCSGG 3 cut(s) 304, 305, 534
NdeII GATC 6 cut(s) 38, 50, 456, 506, 510, 560
NgoMIV GCCGGC 1 cut(s) 517
NlaIV GGNNCC 1 cut(s) 346
NmeAIII GCCGAG 1 cut(s) 410
NmuCI GTSAC 2 cut(s) 95, 402
PaeR7I CTCGAG 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 500
PdiI GCCGGC 1 cut(s) 519
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 544
PinAI ACCGGT 1 cut(s) 280
PkrI GCNGC 3 cut(s) 159, 268, 271
PleI GAGTC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 23
Psp124BI GAGCTC 1 cut(s) 392
Psp6I CCWGG 2 cut(s) 248, 357
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 248, 357
PspN4I GGNNCC 1 cut(s) 346
PspPI GGNCC 6 cut(s) 64, 104, 283, 296, 306, 344
RigI GGCCGGCC 1 cut(s) 521
RsaI GTAC 1 cut(s) 123
RsaNI GTAC 1 cut(s) 122
Rsr2I CGGWCCG 1 cut(s) 283
RsrII CGGWCCG 1 cut(s) 283
SacI GAGCTC 1 cut(s) 392
SacII CCGCGG 1 cut(s) 267
SatI GCNGC 3 cut(s) 158, 267, 270
Sau3AI GATC 6 cut(s) 38, 50, 456, 506, 510, 560
Sau96I GGNCC 6 cut(s) 64, 104, 283, 296, 306, 344
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 5 cut(s) 250, 304, 305, 359, 534
SduI GDGCHC 2 cut(s) 392, 480
SetI ASST 7 cut(s) 159, 194, 335, 354, 392, 404, 412
Sfr274I CTCGAG 1 cut(s) 502
Sfr303I CCGCGG 1 cut(s) 267
SgrBI CCGCGG 1 cut(s) 267
SinI GGWCC 3 cut(s) 283, 296, 344
SlaI CTCGAG 1 cut(s) 502
SmaI CCCGGG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 502
SmoI CTYRAG 1 cut(s) 502
SrfI GCCCGGGC 1 cut(s) 305
SsiI CCGC 5 cut(s) 218, 234, 264, 266, 269
SstI GAGCTC 1 cut(s) 392
StyD4I CCNGG 5 cut(s) 248, 302, 303, 357, 532
StyI CCWWGG 1 cut(s) 82
TaiI ACGT 1 cut(s) 404
TaqI TCGA 4 cut(s) 467, 494, 503, 509
TauI GCSGC 2 cut(s) 269, 272
TfiI GAWTC 1 cut(s) 544
TseFI GTSAC 2 cut(s) 95, 402
TseI GCWGC 1 cut(s) 157
Tsp45I GTSAC 2 cut(s) 95, 402
TspGWI ACGGA 1 cut(s) 8
TspMI CCCGGG 1 cut(s) 303
VpaK11BI GGWCC 3 cut(s) 283, 296, 344
XagI CCTNNNNNAGG 2 cut(s) 247, 356
XhoI CTCGAG 1 cut(s) 502
XmaI CCCGGG 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.