pycom14g06220

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
6038381 .. 6040731
2351 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGTCTAAACATGGAAATGAAGGTCTCAATGAGTTTGACCGGAAAAAGCTTCAAATCGCCACAAACCTGTCAGAAACCCTAGAAATTGGTGGCATGGGCTTTGTTCCTGAGGTTGAGGACAGTCTATTAGTGGTGGTGGTTGGCGGAGTTAGCTTCACGATCGTTGGATTTCCGCCATGGCACCCCCAGAACCCACGAGTTCCATTCTTCAAGGAATCAAGGCTAAATCTAGTGAAACTTGATTGGAAATCGAAGATGGGAGATCATGGAAAAACCAACGAAAATCACGTTGGAATAGCGCGGAACGGGTCTGGTCCACGGGGAGGAACCGAGTCAAGGGTGATCCGGTGCTCCTCCACTATCTCCTTTCCCCTCATTTATTCTTTCCCCCATTGGTCACTCTCTTCTCTCCCTTCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

141

Amino Acids

15.47

Weight (kDa)

8.85

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 180
AccII CGCG 1 cut(s) 301
AciI CCGC 3 cut(s) 144, 173, 301
AclWI GGATC 1 cut(s) 337
AfiI CCNNNNNNNGG 2 cut(s) 323, 336
AgsI TTSAA 2 cut(s) 53, 211
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 2 cut(s) 49, 153
AluI AGCT 2 cut(s) 49, 153
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 1 cut(s) 337
AspLEI GCGC 1 cut(s) 301
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 314
AxyI CCTNAGG 1 cut(s) 108
BanI GGYRCC 1 cut(s) 180
BauI CACGAG 1 cut(s) 195
Bbv12I GWGCWC 1 cut(s) 353
BccI CCATC 1 cut(s) 250
BcoDI GTCTC 1 cut(s) 29
BfaI CTAG 2 cut(s) 80, 230
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 2 cut(s) 182, 328
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 176, 317
BsaWI WCCGGW 2 cut(s) 39, 345
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 336
Bse21I CCTNAGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 176, 317
BseLI CCNNNNNNNGG 2 cut(s) 323, 336
BseMII CTCAG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 301
Bsh1285I CGRYCG 1 cut(s) 162
BshNI GGYRCC 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 2 cut(s) 40, 346
BslI CCNNNNNNNGG 2 cut(s) 323, 336
BsmAI GTCTC 1 cut(s) 29
Bso31I GGTCTC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 3 cut(s) 159, 262, 342
Bsp19I CCATGG 1 cut(s) 176
BspACI CCGC 3 cut(s) 144, 173, 301
BspCNI CTCAG 1 cut(s) 100
BspFNI CGCG 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 182, 328
BspPI GGATC 1 cut(s) 337
BspT107I GGYRCC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 176, 317
BssMI GATC 3 cut(s) 159, 262, 342
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 122
Bst6I CTCTTC 1 cut(s) 409
BstDEI CTNAG 1 cut(s) 108
BstDSI CCRYGG 2 cut(s) 176, 317
BstFNI CGCG 1 cut(s) 301
BstHHI GCGC 1 cut(s) 301
BstKTI GATC 3 cut(s) 162, 265, 345
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 3 cut(s) 159, 262, 342
BstMCI CGRYCG 1 cut(s) 162
BstMWI GCNNNNNNNGC 1 cut(s) 150
BstUI CGCG 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 108
BtgI CCRYGG 2 cut(s) 176, 317
CfoI GCGC 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 314
CviAII CATG 4 cut(s) 11, 94, 177, 266
CviJI RGCY 4 cut(s) 49, 99, 153, 223
CviKI_1 RGCY 4 cut(s) 49, 99, 153, 223
DdeI CTNAG 1 cut(s) 108
DpnI GATC 3 cut(s) 161, 264, 344
DpnII GATC 3 cut(s) 159, 262, 342
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
EciI GGCGGA 2 cut(s) 159, 162
Eco130I CCWWGG 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 1 cut(s) 314
Eco81I CCTNAGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 176
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 4 cut(s) 14, 97, 180, 269
FaiI YATR 4 cut(s) 12, 95, 178, 267
FatI CATG 4 cut(s) 10, 93, 176, 265
FspBI CTAG 2 cut(s) 80, 230
GlaI GCGC 1 cut(s) 300
HapII CCGG 2 cut(s) 40, 346
HhaI GCGC 1 cut(s) 301
Hin1II CATG 4 cut(s) 14, 97, 180, 269
Hin6I GCGC 1 cut(s) 299
HinP1I GCGC 1 cut(s) 299
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 215, 332
HpaII CCGG 2 cut(s) 40, 346
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 2 cut(s) 73, 422
Hpy188III TCNNGA 2 cut(s) 107, 157
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 2 cut(s) 14, 423
HpyCH4III ACNGT 1 cut(s) 122
HpyCH4IV ACGT 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 150
HpyF3I CTNAG 1 cut(s) 108
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 4 cut(s) 14, 97, 180, 269
HspAI GCGC 1 cut(s) 299
Kzo9I GATC 3 cut(s) 159, 262, 342
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 6 cut(s) 53, 80, 120, 200, 297, 359
MaeI CTAG 2 cut(s) 80, 230
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 396
MalI GATC 3 cut(s) 161, 264, 344
MboI GATC 3 cut(s) 159, 262, 342
MboII GAAGA 3 cut(s) 199, 265, 396
MhlI GDGCHC 1 cut(s) 353
MluCI AATT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 2 cut(s) 145, 271
MnlI CCTC 5 cut(s) 103, 109, 317, 364, 383
MslI CAYNNNNRTG 1 cut(s) 15
MspI CCGG 2 cut(s) 40, 346
MvnI CGCG 1 cut(s) 301
MwoI GCNNNNNNNGC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 176
NdeII GATC 3 cut(s) 159, 262, 342
NlaIII CATG 4 cut(s) 14, 97, 180, 269
NlaIV GGNNCC 2 cut(s) 182, 328
NmuCI GTSAC 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PfeI GAWTC 1 cut(s) 215
Ple19I CGATCG 1 cut(s) 162
PleI GAGTC 1 cut(s) 340
PpsI GAGTC 1 cut(s) 340
PspN4I GGNNCC 2 cut(s) 182, 328
PspPI GGNCC 1 cut(s) 314
PvuI CGATCG 1 cut(s) 162
RseI CAYNNNNRTG 1 cut(s) 15
Sau3AI GATC 3 cut(s) 159, 262, 342
Sau96I GGNCC 1 cut(s) 314
SchI GAGTC 1 cut(s) 341
SduI GDGCHC 1 cut(s) 353
SetI ASST 6 cut(s) 25, 51, 69, 114, 155, 291
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 15
Sse9I AATT 1 cut(s) 84
SsiI CCGC 3 cut(s) 144, 173, 301
SspMI CTAG 2 cut(s) 80, 230
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 122
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 251
TasI AATT 1 cut(s) 84
TfiI GAWTC 1 cut(s) 215
TseFI GTSAC 1 cut(s) 396
Tsp45I GTSAC 1 cut(s) 396
TspDTI ATGAA 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 314
XspI CTAG 2 cut(s) 80, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.