pycom14g07510

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Reverse (-)
7677972 .. 7679642
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGCAAATGGTCACAAACTCATTTCTCAGCATTGCCCTGAGTCCAGACGCCCGCCACGCGCACACCCATATCAATGCCTTTCAGATGATCGGAATGTCCTCGTGTCTAGACCTCTCCGGCTTCTTTGAGAATGAGTTGAAACATGCATGTGCTTCCGTTAACTCTCAATTTGGTCTCATGTTTTTGGGGTCTCACTCTCACATTATACCTTTTTCTGGCATTTTTCTTGGCAGTATGGTCTCAAGAGCCGACATGAATGAAGTTTATGCTGAATTTGCCAATTCCATCGACTCCCGAGAAGCCGTCAAGAATTCCCATGTGGAAAGGTGCCCTGGGAGCCTACTTTATCAACGCATTTGCTATTTCCCGGTGGCCCTTCCTTACAGTGTTTTCAGTGCTTCTTCTCTGCATAGATCTCTCCAGCTGGTTGTGCTCAATAGTCAAACGTTAGCAAACAAAATCCACATGCTCAAACTTGTTCTGTACTTCCTTTTCAGGATTCACATTTCTGTGATATTGAGTGAGGATAAAACTGGAAGACTAATGAAATATGACCCGAAAACCAAACAAGTACAATTTGTTCTTGAAAACCTGTCATTCCCAAATGGGGTGGCATTGAGCAAAATGGGGGACTTCATTCTAATTGCAGAGACCACACCTTGCAGGATTCTAAGATAA

Protein Analysis

226

Amino Acids

25.23

Weight (kDa)

8.61

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAF PF03822 22 - 55 1.6e-08 NAF domain
Str_synth PF03088 166 - 225 5.3e-17 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 327
AccII CGCG 1 cut(s) 59
AciI CCGC 1 cut(s) 52
AclI AACGTT 1 cut(s) 446
AcsI RAATTY 2 cut(s) 272, 310
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 2 cut(s) 485, 573
AfiI CCNNNNNNNGG 2 cut(s) 215, 607
AgsI TTSAA 2 cut(s) 139, 587
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 1 cut(s) 424
AluI AGCT 1 cut(s) 424
Alw21I GWGCWC 1 cut(s) 435
Alw26I GTCTC 4 cut(s) 179, 195, 244, 644
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 1 cut(s) 372
ApoI RAATTY 2 cut(s) 272, 310
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 373
AsuC2I CCSGG 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 294
BaeGI GKGCMC 1 cut(s) 332
BanI GGYRCC 1 cut(s) 327
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 544
Bbv12I GWGCWC 1 cut(s) 435
BccI CCATC 1 cut(s) 293
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 1 cut(s) 333
BcnI CCSGG 1 cut(s) 368
BcoDI GTCTC 4 cut(s) 179, 195, 244, 644
BfaI CTAG 1 cut(s) 107
BglII AGATCT 1 cut(s) 413
Bme1390I CCNGG 2 cut(s) 333, 368
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 373
BmiI GGNNCC 2 cut(s) 329, 338
BmrFI CCNGG 2 cut(s) 333, 368
BpiI GAAGAC 1 cut(s) 544
BpmI CTGGAG 1 cut(s) 404
BpuEI CTTGAG 1 cut(s) 226
BpuMI CCSGG 1 cut(s) 368
BsaHI GRCGYC 1 cut(s) 48
BsaI GGTCTC 4 cut(s) 179, 195, 244, 644
BsaJI CCNNGG 2 cut(s) 331, 332
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 607
Bse1I ACTGG 1 cut(s) 538
Bse3DI GCAATG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 331, 332
BseLI CCNNNNNNNGG 2 cut(s) 215, 607
BseMI GCAATG 1 cut(s) 30
BseMII CTCAG 2 cut(s) 29, 40
BseNI ACTGG 1 cut(s) 538
BseSI GKGCMC 1 cut(s) 332
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 1 cut(s) 374
BshNI GGYRCC 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 435
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 2 cut(s) 117, 368
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 2 cut(s) 215, 607
BsmAI GTCTC 4 cut(s) 179, 195, 244, 644
BsmFI GGGAC 1 cut(s) 644
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 4 cut(s) 179, 195, 244, 644
BsoBI CYCGRG 1 cut(s) 294
Bsp1286I GDGCHC 2 cut(s) 332, 435
Bsp143I GATC 2 cut(s) 87, 413
BspACI CCGC 1 cut(s) 52
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 2 cut(s) 30, 39
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 2 cut(s) 329, 338
BspT107I GGYRCC 1 cut(s) 327
BspTNI GGTCTC 4 cut(s) 179, 195, 244, 644
BsrDI GCAATG 1 cut(s) 30
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 2 cut(s) 331, 332
BssMI GATC 2 cut(s) 87, 413
BssNI GRCGYC 1 cut(s) 48
BssSI CACGAG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 52
BstDEI CTNAG 3 cut(s) 26, 38, 671
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 2 cut(s) 90, 416
BstMAI GTCTC 4 cut(s) 179, 195, 244, 644
BstMBI GATC 2 cut(s) 87, 413
BstMWI GCNNNNNNNGC 4 cut(s) 56, 275, 336, 430
BstNI CCWGG 1 cut(s) 333
BstNSI RCATGY 3 cut(s) 146, 150, 469
BstSCI CCNGG 2 cut(s) 331, 366
BstSLI GKGCMC 1 cut(s) 332
BstUI CGCG 1 cut(s) 59
BstV2I GAAGAC 1 cut(s) 544
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuRI GGCC 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 391, 400
Cac8I GCNNGC 1 cut(s) 52
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 373
CseI GACGC 1 cut(s) 56
Csp6I GTAC 2 cut(s) 484, 572
CspCI CAANNNNNGTGG 2 cut(s) 591, 626
CviAII CATG 6 cut(s) 143, 147, 178, 253, 317, 466
CviJI RGCY 6 cut(s) 120, 248, 302, 339, 374, 424
CviKI_1 RGCY 6 cut(s) 120, 248, 302, 339, 374, 424
CviQI GTAC 2 cut(s) 484, 572
DdeI CTNAG 3 cut(s) 26, 38, 671
DpnI GATC 2 cut(s) 89, 415
DpnII GATC 2 cut(s) 87, 413
Eco31I GGTCTC 4 cut(s) 179, 195, 244, 644
Eco88I CYCGRG 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 1 cut(s) 331
EcoT22I ATGCAT 1 cut(s) 148
FaeI CATG 6 cut(s) 146, 150, 181, 256, 320, 469
FaqI GGGAC 1 cut(s) 644
FatI CATG 6 cut(s) 142, 146, 177, 252, 316, 465
FauI CCCGC 1 cut(s) 59
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 60
GsuI CTGGAG 1 cut(s) 404
HaeIII GGCC 1 cut(s) 374
HapII CCGG 2 cut(s) 117, 368
HgaI GACGC 1 cut(s) 56
HhaI GCGC 1 cut(s) 61
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 6 cut(s) 146, 150, 181, 256, 320, 469
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 4 cut(s) 40, 290, 499, 667
HpaI GTTAAC 1 cut(s) 160
HpaII CCGG 2 cut(s) 117, 368
Hpy166II GTNNAC 1 cut(s) 160
Hpy188I TCNGA 2 cut(s) 84, 92
Hpy188III TCNNGA 7 cut(s) 44, 107, 243, 294, 307, 496, 584
Hpy8I GTNNAC 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 386
HpyCH4III ACNGT 1 cut(s) 386
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 5 cut(s) 4, 146, 409, 647, 663
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 275, 336, 430
HpyF3I CTNAG 3 cut(s) 26, 38, 671
HpySE526I ACGT 1 cut(s) 446
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 6 cut(s) 146, 150, 181, 256, 320, 469
HspAI GCGC 1 cut(s) 59
KspAI GTTAAC 1 cut(s) 160
Kzo9I GATC 2 cut(s) 87, 413
LmnI GCTCC 1 cut(s) 336
MaeI CTAG 1 cut(s) 107
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 1 cut(s) 10
MalI GATC 2 cut(s) 89, 415
MboI GATC 2 cut(s) 87, 413
MboII GAAGA 2 cut(s) 393, 549
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 2 cut(s) 332, 435
MluCI AATT 6 cut(s) 167, 272, 280, 310, 575, 642
MlyI GAGTC 2 cut(s) 49, 284
MnlI CCTC 3 cut(s) 109, 122, 517
Mph1103I ATGCAT 1 cut(s) 148
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 3 cut(s) 72, 147, 509
MspA1I CMGCKG 1 cut(s) 424
MspI CCGG 2 cut(s) 117, 368
MspR9I CCNGG 2 cut(s) 333, 368
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 4 cut(s) 56, 275, 336, 430
NciI CCSGG 1 cut(s) 368
NdeII GATC 2 cut(s) 87, 413
NlaIII CATG 6 cut(s) 146, 150, 181, 256, 320, 469
NlaIV GGNNCC 2 cut(s) 329, 338
NmuCI GTSAC 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 148
NspI RCATGY 3 cut(s) 146, 150, 469
PasI CCCWGGG 1 cut(s) 332
PfeI GAWTC 2 cut(s) 499, 667
PleI GAGTC 2 cut(s) 48, 284
PpsI GAGTC 2 cut(s) 48, 284
Psp1406I AACGTT 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 329, 338
PspPI GGNCC 1 cut(s) 373
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
PsuI RGATCY 1 cut(s) 413
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 2 cut(s) 485, 573
RsaNI GTAC 2 cut(s) 484, 572
RseI CAYNNNNRTG 3 cut(s) 72, 147, 509
SaqAI TTAA 1 cut(s) 159
Sau3AI GATC 2 cut(s) 87, 413
Sau96I GGNCC 1 cut(s) 373
SchI GAGTC 2 cut(s) 49, 284
ScrFI CCNGG 2 cut(s) 333, 368
SduI GDGCHC 2 cut(s) 332, 435
SetI ASST 7 cut(s) 114, 211, 329, 426, 449, 594, 661
SmiMI CAYNNNNRTG 3 cut(s) 72, 147, 509
SmlI CTYRAG 1 cut(s) 241
SmoI CTYRAG 1 cut(s) 241
Sse9I AATT 6 cut(s) 167, 272, 280, 310, 575, 642
SsiI CCGC 1 cut(s) 52
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 331, 366
TaaI ACNGT 1 cut(s) 386
TaiI ACGT 1 cut(s) 449
TaqI TCGA 1 cut(s) 288
TasI AATT 6 cut(s) 167, 272, 280, 310, 575, 642
TatI WGTACW 2 cut(s) 483, 571
TfiI GAWTC 2 cut(s) 499, 667
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 2 cut(s) 391, 400
TseFI GTSAC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 10
TspDTI ATGAA 4 cut(s) 269, 273, 560, 625
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 391, 400
XapI RAATTY 2 cut(s) 272, 310
XbaI TCTAGA 1 cut(s) 106
XceI RCATGY 3 cut(s) 146, 150, 469
XspI CTAG 1 cut(s) 107
Zsp2I ATGCAT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.