pycom14g11230

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
14204166 .. 14205375
1210 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGCTTCTGAAGCCTCTGGAATATTGGATGAAACAGGAGTTTTGTTTAGTTCCCCTGCGTGAGCAAGTGAATAATTTTGAGGAAAGCAGAAATAACATGGTGAAAACAATGGGAGAAAATAATACAATGGAATTCTTGAAGAAGACAACCTTCTCGGTCGCAATTGGATTCAATGATGTTCTCAACTATTTCCAACCATCCATACCATTCTTTGGTGATGATAAGCGACTGCACGAGCTGGGAGCTAGAAAGTTCGTTGTGGCAGGGATTGGACCCCTAGGATGCATACCATTTATTCGTGCCATCAGTTTGAGTGGACGGTGTTTTGTTGAGGTGAATGAAATAATCCAAGGTTACAATATGAAACTTAATGGGGTTTTAGATCAACTTAATCAAGAGCTGGGTCCTGAAGCGATCTTTCTCTATGCAAACTCCTTCGATATCTTCATGAAGATTATAGTAAACTATCATCAATATGGGTGTGCCAATGTAAATGAACCATGCTGTGGAGGGTACTTTCCGCTGTTTGTTTGCTTCAAGAGCCGTGATGCAAACAGAAGCTCTGCATTGTGTGACGATCGATCGAAGTACGTGTTTTGGGATGCATATCATCCCACAGAAGCTGGTAATATGATAATAGCGGAGCGGTTACTTGATGGAGATATGAAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

225

Amino Acids

25.63

Weight (kDa)

5.47

Isoelectric Point (pI)

29.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 217 7.3e-15 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 212, 506
AccBSI CCGCTC 1 cut(s) 646
AciI CCGC 3 cut(s) 521, 641, 646
AcsI RAATTY 1 cut(s) 131
AcuI CTGAAG 2 cut(s) 29, 429
AfaI GTAC 2 cut(s) 515, 590
AfiI CCNNNNNNNGG 2 cut(s) 212, 506
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 3 cut(s) 139, 172, 538
AluBI AGCT 5 cut(s) 238, 245, 400, 561, 623
AluI AGCT 5 cut(s) 238, 245, 400, 561, 623
AlwNI CAGNNNCTG 1 cut(s) 623
ApoI RAATTY 1 cut(s) 131
AspA2I CCTAGG 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 272, 404
AsuHPI GGTGA 3 cut(s) 112, 227, 346
AvaII GGWCC 2 cut(s) 272, 404
AvrII CCTAGG 1 cut(s) 277
BauI CACGAG 1 cut(s) 233
BbsI GAAGAC 1 cut(s) 149
BccI CCATC 3 cut(s) 205, 311, 650
BceAI ACGGC 1 cut(s) 528
BfaI CTAG 2 cut(s) 246, 278
BlnI CCTAGG 1 cut(s) 277
Bme18I GGWCC 2 cut(s) 272, 404
BmgT120I GGNCC 2 cut(s) 272, 404
BmiI GGNNCC 2 cut(s) 274, 405
BmsI GCATC 3 cut(s) 272, 538, 592
BpiI GAAGAC 1 cut(s) 149
Bsa29I ATCGAT 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 606
BsaJI CCNNGG 2 cut(s) 277, 349
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 506
Bse8I GATNNNNATC 1 cut(s) 606
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 2 cut(s) 277, 349
BseGI GGATG 5 cut(s) 33, 197, 287, 607, 610
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 2 cut(s) 212, 506
BseYI CCCAGC 2 cut(s) 238, 400
BsgI GTGCAG 1 cut(s) 215
Bsh1285I CGRYCG 3 cut(s) 159, 580, 584
BshVI ATCGAT 1 cut(s) 580
BsiEI CGRYCG 3 cut(s) 159, 580, 584
BslI CCNNNNNNNGG 2 cut(s) 212, 506
Bsp143I GATC 4 cut(s) 382, 414, 577, 581
BspACI CCGC 3 cut(s) 521, 641, 646
BspDI ATCGAT 1 cut(s) 580
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 2 cut(s) 274, 405
BsrBI CCGCTC 1 cut(s) 646
BssECI CCNNGG 2 cut(s) 277, 349
BssMI GATC 4 cut(s) 382, 414, 577, 581
BssSI CACGAG 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 277, 349
Bst2BI CACGAG 1 cut(s) 233
Bst4CI ACNGT 1 cut(s) 321
BstBAI YACGTR 1 cut(s) 592
BstF5I GGATG 5 cut(s) 33, 197, 287, 607, 610
BstKTI GATC 4 cut(s) 385, 417, 580, 584
BstMBI GATC 4 cut(s) 382, 414, 577, 581
BstMCI CGRYCG 3 cut(s) 159, 580, 584
BstMWI GCNNNNNNNGC 2 cut(s) 10, 540
BstV2I GAAGAC 1 cut(s) 149
Bsu15I ATCGAT 1 cut(s) 580
BsuTUI ATCGAT 1 cut(s) 580
BtsCI GGATG 5 cut(s) 33, 197, 287, 607, 610
CaiI CAGNNNCTG 1 cut(s) 623
CciI TCATGA 1 cut(s) 447
Cfr13I GGNCC 2 cut(s) 272, 404
ClaI ATCGAT 1 cut(s) 580
Csp6I GTAC 2 cut(s) 514, 589
CviAII CATG 3 cut(s) 97, 448, 501
CviJI RGCY 7 cut(s) 13, 238, 245, 400, 543, 561, 623
CviKI_1 RGCY 7 cut(s) 13, 238, 245, 400, 543, 561, 623
CviQI GTAC 2 cut(s) 514, 589
DpnI GATC 4 cut(s) 384, 416, 579, 583
DpnII GATC 4 cut(s) 382, 414, 577, 581
Eco130I CCWWGG 2 cut(s) 277, 349
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 272, 404
Eco57I CTGAAG 2 cut(s) 29, 429
EcoO109I RGGNCCY 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 131
EcoRV GATATC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 277, 349
EcoT22I ATGCAT 2 cut(s) 287, 607
ErhI CCWWGG 2 cut(s) 277, 349
FaeI CATG 3 cut(s) 100, 451, 504
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FatI CATG 3 cut(s) 96, 447, 500
FokI GGATG 5 cut(s) 40, 184, 294, 597, 614
FspBI CTAG 2 cut(s) 246, 278
GsaI CCCAGC 2 cut(s) 242, 404
Hin1II CATG 3 cut(s) 100, 451, 504
HinfI GANTC 1 cut(s) 168
HphI GGTGA 3 cut(s) 112, 227, 346
Hpy166II GTNNAC 2 cut(s) 317, 463
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 6 cut(s) 17, 136, 395, 407, 448, 538
Hpy8I GTNNAC 2 cut(s) 317, 463
HpyAV CCTTC 2 cut(s) 160, 445
HpyCH4III ACNGT 1 cut(s) 321
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 6 cut(s) 232, 285, 428, 551, 566, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 540
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 3 cut(s) 100, 451, 504
Kzo9I GATC 4 cut(s) 382, 414, 577, 581
LmnI GCTCC 2 cut(s) 242, 643
LpnPI CCDG 8 cut(s) 2, 20, 68, 224, 249, 386, 420, 609
LweI GCATC 3 cut(s) 272, 538, 592
MaeI CTAG 2 cut(s) 246, 278
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 3 cut(s) 353, 572, 648
MalI GATC 4 cut(s) 384, 416, 579, 583
MbiI CCGCTC 1 cut(s) 646
MboI GATC 4 cut(s) 382, 414, 577, 581
MboII GAAGA 4 cut(s) 151, 154, 436, 463
MfeI CAATTG 1 cut(s) 162
MluCI AATT 3 cut(s) 73, 131, 162
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 4 cut(s) 24, 73, 325, 503
Mph1103I ATGCAT 2 cut(s) 287, 607
MseI TTAA 2 cut(s) 369, 390
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 523
MunI CAATTG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 10, 540
NdeII GATC 4 cut(s) 382, 414, 577, 581
NlaIII CATG 3 cut(s) 100, 451, 504
NlaIV GGNNCC 2 cut(s) 274, 405
NmuCI GTSAC 1 cut(s) 572
NsiI ATGCAT 2 cut(s) 287, 607
PagI TCATGA 1 cut(s) 447
PfeI GAWTC 1 cut(s) 168
PflMI CCANNNNNTGG 2 cut(s) 212, 506
Ple19I CGATCG 2 cut(s) 580, 584
Ppu21I YACGTR 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 404
PspFI CCCAGC 2 cut(s) 238, 400
PspN4I GGNNCC 2 cut(s) 274, 405
PspPI GGNCC 2 cut(s) 272, 404
PspPPI RGGWCCY 1 cut(s) 404
PstNI CAGNNNCTG 1 cut(s) 623
PvuI CGATCG 2 cut(s) 580, 584
RsaI GTAC 2 cut(s) 515, 590
RsaNI GTAC 2 cut(s) 514, 589
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 2 cut(s) 369, 390
Sau3AI GATC 4 cut(s) 382, 414, 577, 581
Sau96I GGNCC 2 cut(s) 272, 404
SetI ASST 9 cut(s) 152, 240, 247, 336, 355, 402, 563, 594, 625
SfaNI GCATC 3 cut(s) 272, 538, 592
SinI GGWCC 2 cut(s) 272, 404
SmiMI CAYNNNNRTG 1 cut(s) 474
Sse9I AATT 3 cut(s) 73, 131, 162
SsiI CCGC 3 cut(s) 521, 641, 646
SspI AATATT 1 cut(s) 23
SspMI CTAG 2 cut(s) 246, 278
StyI CCWWGG 2 cut(s) 277, 349
TaaI ACNGT 1 cut(s) 321
TaiI ACGT 1 cut(s) 594
TaqI TCGA 3 cut(s) 438, 580, 584
TaqII GACCGA 1 cut(s) 145
TasI AATT 3 cut(s) 73, 131, 162
TfiI GAWTC 1 cut(s) 168
Tru1I TTAA 2 cut(s) 369, 390
Tru9I TTAA 2 cut(s) 369, 390
TseFI GTSAC 1 cut(s) 572
Tsp45I GTSAC 1 cut(s) 572
TspDTI ATGAA 6 cut(s) 44, 354, 377, 436, 464, 510
Van91I CCANNNNNTGG 2 cut(s) 212, 506
VpaK11BI GGWCC 2 cut(s) 272, 404
XapI RAATTY 1 cut(s) 131
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 2 cut(s) 246, 278
Zsp2I ATGCAT 2 cut(s) 287, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.