pycom14g13950

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Reverse (-)
16758688 .. 16759902
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
ATGATCTTTATCCCATTATTCAAGCCTTGCTTAACGTTTAATCATGCTAATTATCCCCTCTCTCTTTATATATACTCCCCCCTTCCTCACCTCCTTCCATTCACAAACACACTTCCTCTCTCTCTCCCTCACCTTCTCTCCCTGTGCTTGTCTCAGTGCCTTGCACATTCAAAAAAAGACCAAAAAATGGCAAGGCCCTCCTTAGGCCCCTCACTTCTCTCTCTCCTCCTCTTCTCTCTCCTCTCCCCAACCCTCATTTCCTCCCGGCCACTTCATCTTCAAGACCCTGAATTGGTAGTACAAGAGGTACAAAGGAACATTAGTGACTCAGTGTCTAGGAGGAACTTGGGCTACTTATCATGTGGGACTGGAAACCCTATCGACGACTGCTGGCGGTGCGATCCGAACTGGGAGAAGAACAGGCAGCGTCTAGCAGATTGCGCCATAGGGTTCGGGAAAAACTCCATCGGTGGAAGAGACGGGAAGATTTACGTGGTCACAGATTCCGGCGACGACAACCCCGTGAACCCCAAGCCAGGAACCCTACGACACGCCGTCATCCAAGACGAGCCGTTATGGATCCCGACATATTTTCCAGCGCGACATGACCATCCAGCTCAAGGAGGAGCTGATCATGAACTCCTTCAAGACAATCGACGGCCGGGGAGCGTCCGTCCACATTGCCGGCGGGCCATGCATCACCATCCAGTACGTGACCAACATTATTATCCACGGACTGAACATACACGATTGCAAGCAGGGTGGGAACGCTATGGTGAGGGACTCCCCAGGGCACTTCGGGTGGAGAACCATATCGGACGGCGACGGCGTGTCGATCGTTCGGTGGGAGCCACGTGTGGGAAGACCATTGCTCGTTGTCCAACTGCAACGATGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

300

Amino Acids

33.72

Weight (kDa)

9.33

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 187
AccII CGCG 1 cut(s) 601
AciI CCGC 2 cut(s) 394, 688
AclI AACGTT 1 cut(s) 35
AclWI GGATC 3 cut(s) 395, 574, 587
AcoI YGGCCR 2 cut(s) 266, 659
AcvI CACGTG 1 cut(s) 855
AfaI GTAC 3 cut(s) 300, 309, 711
AfiI CCNNNNNNNGG 6 cut(s) 187, 203, 292, 536, 620, 858
AflIII ACRYGT 1 cut(s) 854
AgsI TTSAA 4 cut(s) 22, 171, 281, 647
AhdI GACNNNNNGTC 3 cut(s) 331, 554, 830
AjnI CCWGG 2 cut(s) 535, 788
AjuI GAANNNNNNNTTGG 2 cut(s) 241, 273
AluBI AGCT 2 cut(s) 617, 629
AluI AGCT 2 cut(s) 617, 629
Alw26I GTCTC 2 cut(s) 156, 471
AlwI GGATC 3 cut(s) 395, 574, 587
AoxI GGCC 5 cut(s) 194, 205, 266, 659, 690
ApeKI GCWGC 1 cut(s) 424
Asp700I GAANNNNTTC 1 cut(s) 642
AspLEI GCGC 2 cut(s) 443, 601
AspS9I GGNCC 3 cut(s) 195, 206, 690
AsuC2I CCSGG 2 cut(s) 265, 663
AsuHPI GGTGA 4 cut(s) 80, 122, 692, 788
AxyI CCTNAGG 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 796
BamHI GGATCC 1 cut(s) 579
BbrPI CACGTG 1 cut(s) 855
BbsI GAAGAC 1 cut(s) 869
BbvI GCAGC 1 cut(s) 436
BccI CCATC 4 cut(s) 473, 618, 711, 886
BceAI ACGGC 5 cut(s) 539, 556, 674, 836, 842
BciT130I CCWGG 2 cut(s) 537, 790
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 2 cut(s) 265, 663
BcoDI GTCTC 2 cut(s) 156, 471
BfaI CTAG 2 cut(s) 336, 431
BisI GCNGC 1 cut(s) 425
BlsI GCNGC 1 cut(s) 426
Bme1390I CCNGG 4 cut(s) 265, 537, 663, 790
BmeRI GACNNNNNGTC 3 cut(s) 331, 554, 830
BmgT120I GGNCC 3 cut(s) 195, 206, 690
BmiI GGNNCC 4 cut(s) 208, 541, 581, 850
BmrFI CCNGG 4 cut(s) 265, 537, 663, 790
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 1 cut(s) 706
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 869
BpuEI CTTGAG 1 cut(s) 603
BpuMI CCSGG 2 cut(s) 265, 663
BsaAI YACGTR 3 cut(s) 493, 713, 855
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 4 cut(s) 662, 731, 788, 789
BsaXI ACNNNNNCTCC 4 cut(s) 122, 152, 658, 688
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 203, 292, 536, 620, 858
Bse118I RCCGGY 1 cut(s) 684
Bse1I ACTGG 3 cut(s) 373, 413, 707
Bse21I CCTNAGG 1 cut(s) 202
Bse3DI GCAATG 2 cut(s) 679, 867
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 2 cut(s) 537, 790
BseDI CCNNGG 4 cut(s) 662, 731, 788, 789
BseGI GGATG 3 cut(s) 558, 610, 703
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 6 cut(s) 187, 203, 292, 536, 620, 858
BseMI GCAATG 2 cut(s) 679, 867
BseMII CTCAG 2 cut(s) 167, 342
BseNI ACTGG 3 cut(s) 373, 413, 707
BseRI GAGGAG 4 cut(s) 215, 218, 230, 639
BseSI GKGCMC 1 cut(s) 796
BseX3I CGGCCG 1 cut(s) 659
BseXI GCAGC 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 601
Bsh1285I CGRYCG 2 cut(s) 662, 838
BshFI GGCC 5 cut(s) 196, 207, 268, 661, 692
BsiEI CGRYCG 2 cut(s) 662, 838
BsiSI CCGG 4 cut(s) 265, 507, 662, 685
BslFI GGGAC 2 cut(s) 379, 795
BslI CCNNNNNNNGG 6 cut(s) 187, 203, 292, 536, 620, 858
BsmAI GTCTC 2 cut(s) 156, 471
BsmBI CGTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 379, 795
BsnI GGCC 5 cut(s) 196, 207, 268, 661, 692
Bsp1286I GDGCHC 1 cut(s) 796
Bsp143I GATC 5 cut(s) 3, 400, 579, 631, 835
BspACI CCGC 2 cut(s) 394, 688
BspANI GGCC 5 cut(s) 196, 207, 268, 661, 692
BspCNI CTCAG 2 cut(s) 166, 341
BspFNI CGCG 1 cut(s) 601
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 4 cut(s) 208, 541, 581, 850
BspPI GGATC 3 cut(s) 395, 574, 587
BsrDI GCAATG 2 cut(s) 679, 867
BsrFI RCCGGY 1 cut(s) 684
BsrI ACTGG 3 cut(s) 373, 413, 707
BssAI RCCGGY 1 cut(s) 684
BssECI CCNNGG 4 cut(s) 662, 731, 788, 789
BssMI GATC 5 cut(s) 3, 400, 579, 631, 835
Bst2UI CCWGG 2 cut(s) 537, 790
Bst6I CTCTTC 2 cut(s) 236, 469
BstBAI YACGTR 3 cut(s) 493, 713, 855
BstC8I GCNNGC 4 cut(s) 392, 686, 690, 756
BstDEI CTNAG 3 cut(s) 153, 202, 328
BstDSI CCRYGG 1 cut(s) 731
BstF5I GGATG 3 cut(s) 558, 610, 703
BstFNI CGCG 1 cut(s) 601
BstHHI GCGC 2 cut(s) 443, 601
BstKTI GATC 5 cut(s) 6, 403, 582, 634, 838
BstMAI GTCTC 2 cut(s) 156, 471
BstMBI GATC 5 cut(s) 3, 400, 579, 631, 835
BstMCI CGRYCG 2 cut(s) 662, 838
BstMWI GCNNNNNNNGC 3 cut(s) 396, 440, 694
BstNI CCWGG 2 cut(s) 537, 790
BstSCI CCNGG 4 cut(s) 263, 535, 661, 788
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 1 cut(s) 601
BstV1I GCAGC 1 cut(s) 436
BstV2I GAAGAC 1 cut(s) 869
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BstZI CGGCCG 1 cut(s) 659
Bsu36I CCTNAGG 1 cut(s) 202
BsuRI GGCC 5 cut(s) 196, 207, 268, 661, 692
BtgI CCRYGG 1 cut(s) 731
BtsCI GGATG 3 cut(s) 558, 610, 703
BtsIMutI CAGTG 2 cut(s) 161, 336
Cac8I GCNNGC 4 cut(s) 392, 686, 690, 756
CciI TCATGA 1 cut(s) 634
CfoI GCGC 2 cut(s) 443, 601
Cfr10I RCCGGY 1 cut(s) 684
Cfr13I GGNCC 3 cut(s) 195, 206, 690
CseI GACGC 2 cut(s) 416, 658
Csp6I GTAC 3 cut(s) 299, 308, 710
CspCI CAANNNNNGTGG 2 cut(s) 743, 778
CviAII CATG 5 cut(s) 44, 360, 605, 635, 694
CviQI GTAC 3 cut(s) 299, 308, 710
DdeI CTNAG 3 cut(s) 153, 202, 328
DpnI GATC 5 cut(s) 5, 402, 581, 633, 837
DpnII GATC 5 cut(s) 3, 400, 579, 631, 835
DriI GACNNNNNGTC 3 cut(s) 331, 554, 830
EaeI YGGCCR 2 cut(s) 266, 659
EagI CGGCCG 1 cut(s) 659
Eam1104I CTCTTC 2 cut(s) 236, 469
Eam1105I GACNNNNNGTC 3 cut(s) 331, 554, 830
EarI CTCTTC 2 cut(s) 236, 469
EclXI CGGCCG 1 cut(s) 659
Eco52I CGGCCG 1 cut(s) 659
Eco72I CACGTG 1 cut(s) 855
Eco81I CCTNAGG 1 cut(s) 202
EcoO109I RGGNCCY 2 cut(s) 195, 206
EcoRII CCWGG 2 cut(s) 535, 788
EcoT22I ATGCAT 1 cut(s) 699
Esp3I CGTCTC 1 cut(s) 471
FaeI CATG 5 cut(s) 47, 363, 608, 638, 697
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 2 cut(s) 379, 795
FatI CATG 5 cut(s) 43, 359, 604, 634, 693
FauI CCCGC 1 cut(s) 681
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 1 cut(s) 425
FokI GGATG 3 cut(s) 545, 597, 690
Fsp4HI GCNGC 1 cut(s) 425
FspBI CTAG 2 cut(s) 336, 431
GlaI GCGC 2 cut(s) 442, 600
GluI GCNGC 1 cut(s) 425
HaeIII GGCC 5 cut(s) 196, 207, 268, 661, 692
HapII CCGG 4 cut(s) 265, 507, 662, 685
HgaI GACGC 2 cut(s) 416, 658
HhaI GCGC 2 cut(s) 443, 601
Hin1II CATG 5 cut(s) 47, 363, 608, 638, 697
Hin6I GCGC 2 cut(s) 441, 599
HinP1I GCGC 2 cut(s) 441, 599
HinfI GANTC 3 cut(s) 326, 503, 783
HpaII CCGG 4 cut(s) 265, 507, 662, 685
HphI GGTGA 4 cut(s) 80, 122, 692, 788
Hpy166II GTNNAC 2 cut(s) 526, 677
Hpy188I TCNGA 2 cut(s) 405, 818
Hpy188III TCNNGA 5 cut(s) 281, 454, 583, 635, 647
Hpy8I GTNNAC 2 cut(s) 526, 677
Hpy99I CGWCG 4 cut(s) 386, 515, 660, 828
HpyAV CCTTC 4 cut(s) 92, 104, 143, 653
HpyCH4IV ACGT 4 cut(s) 35, 492, 712, 854
HpyCH4V TGCA 4 cut(s) 164, 697, 754, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 396, 440, 694
HpyF3I CTNAG 3 cut(s) 153, 202, 328
HpySE526I ACGT 4 cut(s) 35, 492, 712, 854
Hsp92II CATG 5 cut(s) 47, 363, 608, 638, 697
HspAI GCGC 2 cut(s) 441, 599
KroI GCCGGC 1 cut(s) 684
KroNI GCCGGC 1 cut(s) 686
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 5 cut(s) 3, 400, 579, 631, 835
LmnI GCTCC 3 cut(s) 626, 666, 848
Lsp1109I GCAGC 1 cut(s) 436
LweI GCATC 1 cut(s) 706
MaeI CTAG 2 cut(s) 336, 431
MaeII ACGT 4 cut(s) 35, 492, 712, 854
MaeIII GTNAC 3 cut(s) 323, 496, 713
MalI GATC 5 cut(s) 5, 402, 581, 633, 837
MboI GATC 5 cut(s) 3, 400, 579, 631, 835
MboII GAAGA 6 cut(s) 223, 269, 427, 486, 496, 874
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 796
MluCI AATT 2 cut(s) 49, 290
MlyI GAGTC 2 cut(s) 320, 777
Mph1103I ATGCAT 1 cut(s) 699
MroNI GCCGGC 1 cut(s) 684
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 2 cut(s) 32, 39
MspI CCGG 4 cut(s) 265, 507, 662, 685
MspR9I CCNGG 4 cut(s) 265, 537, 663, 790
MvaI CCWGG 2 cut(s) 537, 790
MvnI CGCG 1 cut(s) 601
MwoI GCNNNNNNNGC 3 cut(s) 396, 440, 694
NaeI GCCGGC 1 cut(s) 686
NciI CCSGG 2 cut(s) 265, 663
NdeII GATC 5 cut(s) 3, 400, 579, 631, 835
NgoMIV GCCGGC 1 cut(s) 684
NlaIII CATG 5 cut(s) 47, 363, 608, 638, 697
NlaIV GGNNCC 4 cut(s) 208, 541, 581, 850
NmuCI GTSAC 3 cut(s) 323, 496, 713
NsiI ATGCAT 1 cut(s) 699
PagI TCATGA 1 cut(s) 634
PasI CCCWGGG 1 cut(s) 789
PcsI WCGNNNNNNNCGW 2 cut(s) 519, 826
PdiI GCCGGC 1 cut(s) 686
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 1 cut(s) 503
PflMI CCANNNNNTGG 1 cut(s) 187
PkrI GCNGC 1 cut(s) 426
Ple19I CGATCG 1 cut(s) 838
PleI GAGTC 2 cut(s) 320, 777
PmaCI CACGTG 1 cut(s) 855
PmlI CACGTG 1 cut(s) 855
PpsI GAGTC 2 cut(s) 320, 777
Ppu21I YACGTR 3 cut(s) 493, 713, 855
Psp1406I AACGTT 1 cut(s) 35
Psp6I CCWGG 2 cut(s) 535, 788
PspCI CACGTG 1 cut(s) 855
PspGI CCWGG 2 cut(s) 535, 788
PspN4I GGNNCC 4 cut(s) 208, 541, 581, 850
PspPI GGNCC 3 cut(s) 195, 206, 690
PsrI GAACNNNNNNTAC 2 cut(s) 335, 367
PsuI RGATCY 1 cut(s) 579
PvuI CGATCG 1 cut(s) 838
RsaI GTAC 3 cut(s) 300, 309, 711
RsaNI GTAC 3 cut(s) 299, 308, 710
SaqAI TTAA 2 cut(s) 32, 39
SatI GCNGC 1 cut(s) 425
Sau3AI GATC 5 cut(s) 3, 400, 579, 631, 835
Sau96I GGNCC 3 cut(s) 195, 206, 690
SchI GAGTC 2 cut(s) 320, 777
ScrFI CCNGG 4 cut(s) 265, 537, 663, 790
SduI GDGCHC 1 cut(s) 796
SetI ASST 9 cut(s) 38, 93, 135, 309, 495, 619, 631, 715, 857
SfaNI GCATC 1 cut(s) 706
SmlI CTYRAG 1 cut(s) 618
SmoI CTYRAG 1 cut(s) 618
Sse9I AATT 2 cut(s) 49, 290
SsiI CCGC 2 cut(s) 394, 688
SspMI CTAG 2 cut(s) 336, 431
StyD4I CCNGG 4 cut(s) 263, 535, 661, 788
TaiI ACGT 4 cut(s) 38, 495, 715, 857
TaqI TCGA 3 cut(s) 381, 655, 834
TasI AATT 2 cut(s) 49, 290
TatI WGTACW 1 cut(s) 298
TfiI GAWTC 1 cut(s) 503
Tru1I TTAA 2 cut(s) 32, 39
Tru9I TTAA 2 cut(s) 32, 39
TscAI CASTG 2 cut(s) 161, 336
TseFI GTSAC 3 cut(s) 323, 496, 713
TseI GCWGC 1 cut(s) 424
Tsp45I GTSAC 3 cut(s) 323, 496, 713
TspDTI ATGAA 2 cut(s) 263, 651
TspGWI ACGGA 2 cut(s) 662, 748
TspRI CASTG 2 cut(s) 161, 336
Van91I CCANNNNNTGG 1 cut(s) 187
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 2 cut(s) 336, 431
Zsp2I ATGCAT 1 cut(s) 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.