pycom14g15850

Transcription initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
N/A
Physical Location & Seq
Forward (+)
18323442 .. 18324407
966 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGTTTATTCGCACGGGATTTACTAAACTTGTGTTCCGACCAGAAATATGCACTGCAGTCAGTGGAGCTACCAAGAAGGAAATTGGACGGGCAAAAGAGTTCATTTTGAAACACCTGGAGGCCGAGCTTGGTCAGGAAATGGGAACTATCCATGCTGGAGATTATTTGGTGAGTTTTTTTATTTATTTTTTTATTTTTTATATTACCCGTCTCTTCGCGTTTCAATATTTTATTTTCTTGTTGGAATGTGTTTTGCTTGAGAACATAATTATCGCATTTTCTCTGCAGAGACGCTTTTGTTCCAATCTTGGAATGCAGAATCGAGATGTCAAGGCTGCCCAAGAAATGGTGCAGAACATTGAAGAGCTTGTTATAAGGTTGTCCTCCGCCAGTTCACAACAATCTTGTCCTGTCTTCTGCAGGAGGAGCCCCATATCGGTGGCAGCTGCCGTTATTTATACCATTAATCAACTATCAGATGAGAAAATGTCTCTTAAAGAGATCTCGGTTGCTACTCAAGTTGCTGAAGGGACGATCAAGAACTCCTACAAAGACCTGCATCCTCATCTAGCAAGGTTAATACCGAACTGGTTCGCCAACGAGGAATGTGTCAAGAATCTGCAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIB TF_Zn_Ribbon

Protein Analysis

210

Amino Acids

23.88

Weight (kDa)

8.2

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIB PF00382 142 - 187 1.4e-07 Transcription factor TFIIB repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
Acc36I ACCTGC 1 cut(s) 564
AccB7I CCANNNNNTGG 1 cut(s) 346
AccII CGCG 1 cut(s) 218
AciI CCGC 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 546
AfiI CCNNNNNNNGG 2 cut(s) 346, 436
AgsI TTSAA 3 cut(s) 109, 224, 362
AjnI CCWGG 1 cut(s) 114
AluBI AGCT 4 cut(s) 68, 127, 367, 446
AluI AGCT 4 cut(s) 68, 127, 367, 446
Alw26I GTCTC 3 cut(s) 215, 283, 495
AoxI GGCC 1 cut(s) 120
ApeKI GCWGC 4 cut(s) 335, 443, 446, 622
AseI ATTAAT 1 cut(s) 465
Asp700I GAANNNNTTC 1 cut(s) 590
AsuHPI GGTGA 1 cut(s) 181
BanII GRGCYC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 406
BbvI GCAGC 3 cut(s) 322, 433, 455
BceAI ACGGC 1 cut(s) 434
BciT130I CCWGG 1 cut(s) 116
BcoDI GTCTC 3 cut(s) 215, 283, 495
BfaI CTAG 2 cut(s) 569, 628
BfmI CTRYAG 4 cut(s) 54, 284, 418, 620
BfuAI ACCTGC 1 cut(s) 564
BglII AGATCT 1 cut(s) 501
BisI GCNGC 4 cut(s) 336, 444, 447, 623
BlsI GCNGC 4 cut(s) 337, 445, 448, 624
Bme1390I CCNGG 1 cut(s) 116
BmiI GGNNCC 1 cut(s) 428
BmrFI CCNGG 1 cut(s) 116
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 2 cut(s) 137, 177
BpuEI CTTGAG 2 cut(s) 278, 501
Bsc4I CCNNNNNNNGG 2 cut(s) 346, 436
Bse1I ACTGG 2 cut(s) 390, 593
BseBI CCWGG 1 cut(s) 116
BseGI GGATG 1 cut(s) 559
BseLI CCNNNNNNNGG 2 cut(s) 346, 436
BseNI ACTGG 2 cut(s) 390, 593
BseRI GAGGAG 1 cut(s) 439
BseXI GCAGC 3 cut(s) 322, 433, 455
BsgI GTGCAG 1 cut(s) 371
Bsh1236I CGCG 1 cut(s) 218
BshFI GGCC 1 cut(s) 122
BslFI GGGAC 1 cut(s) 544
BslI CCNNNNNNNGG 2 cut(s) 346, 436
BsmAI GTCTC 3 cut(s) 215, 283, 495
BsmBI CGTCTC 2 cut(s) 215, 283
BsmFI GGGAC 1 cut(s) 544
BsmI GAATGC 1 cut(s) 318
BsnI GGCC 1 cut(s) 122
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 2 cut(s) 501, 534
BspACI CCGC 1 cut(s) 387
BspANI GGCC 1 cut(s) 122
BspFNI CGCG 1 cut(s) 218
BspLI GGNNCC 1 cut(s) 428
BspMAI CTGCAG 4 cut(s) 58, 288, 422, 624
BspMI ACCTGC 1 cut(s) 564
BspQI GCTCTTC 1 cut(s) 357
BsrI ACTGG 2 cut(s) 390, 593
BssMI GATC 2 cut(s) 501, 534
Bst2UI CCWGG 1 cut(s) 116
Bst6I CTCTTC 2 cut(s) 218, 357
BstF5I GGATG 1 cut(s) 559
BstFNI CGCG 1 cut(s) 218
BstKTI GATC 2 cut(s) 504, 537
BstMAI GTCTC 3 cut(s) 215, 283, 495
BstMBI GATC 2 cut(s) 501, 534
BstMWI GCNNNNNNNGC 1 cut(s) 426
BstNI CCWGG 1 cut(s) 116
BstSCI CCNGG 1 cut(s) 114
BstSFI CTRYAG 4 cut(s) 54, 284, 418, 620
BstUI CGCG 1 cut(s) 218
BstV1I GCAGC 3 cut(s) 322, 433, 455
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 501
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 501
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 1 cut(s) 559
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 67
BveI ACCTGC 1 cut(s) 564
CseI GACGC 1 cut(s) 300
CviAII CATG 1 cut(s) 152
CviJI RGCY 8 cut(s) 68, 122, 127, 335, 367, 429, 446, 625
CviKI_1 RGCY 8 cut(s) 68, 122, 127, 335, 367, 429, 446, 625
DpnI GATC 2 cut(s) 503, 536
DpnII GATC 2 cut(s) 501, 534
Eam1104I CTCTTC 2 cut(s) 218, 357
EarI CTCTTC 2 cut(s) 218, 357
EciI GGCGGA 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 431
Esp3I CGTCTC 2 cut(s) 215, 283
FaeI CATG 1 cut(s) 155
FaiI YATR 7 cut(s) 49, 153, 201, 266, 374, 434, 459
FaqI GGGAC 1 cut(s) 544
FatI CATG 1 cut(s) 151
Fnu4HI GCNGC 4 cut(s) 336, 444, 447, 623
FokI GGATG 1 cut(s) 546
FriOI GRGCYC 1 cut(s) 431
Fsp4HI GCNGC 4 cut(s) 336, 444, 447, 623
FspBI CTAG 2 cut(s) 569, 628
GluI GCNGC 4 cut(s) 336, 444, 447, 623
GsuI CTGGAG 2 cut(s) 137, 177
HaeIII GGCC 1 cut(s) 122
HgaI GACGC 1 cut(s) 300
Hin1II CATG 1 cut(s) 155
HinfI GANTC 2 cut(s) 319, 616
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 2 cut(s) 38, 478
Hpy188III TCNNGA 4 cut(s) 134, 323, 538, 613
Hpy8I GTNNAC 1 cut(s) 395
HpyAV CCTTC 2 cut(s) 70, 521
HpyCH4V TGCA 8 cut(s) 51, 56, 286, 316, 352, 420, 559, 622
HpyF10VI GCNNNNNNNGC 1 cut(s) 426
Hsp92II CATG 1 cut(s) 155
Kzo9I GATC 2 cut(s) 501, 534
LguI GCTCTTC 1 cut(s) 357
LmnI GCTCC 2 cut(s) 65, 426
Lsp1109I GCAGC 3 cut(s) 322, 433, 455
LweI GCATC 1 cut(s) 568
MaeI CTAG 2 cut(s) 569, 628
MalI GATC 2 cut(s) 503, 536
MboI GATC 2 cut(s) 501, 534
MboII GAAGA 3 cut(s) 205, 374, 406
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 1 cut(s) 431
MluCI AATT 2 cut(s) 81, 267
MmeI TCCRAC 2 cut(s) 61, 222
MnlI CCTC 5 cut(s) 112, 394, 417, 573, 595
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 3 cut(s) 465, 495, 578
MslI CAYNNNNRTG 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 446
MspR9I CCNGG 1 cut(s) 116
Mva1269I GAATGC 1 cut(s) 318
MvaI CCWGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 218
MwoI GCNNNNNNNGC 1 cut(s) 426
NdeII GATC 2 cut(s) 501, 534
NlaIII CATG 1 cut(s) 155
NlaIV GGNNCC 1 cut(s) 428
NmeAIII GCCGAG 1 cut(s) 148
PciSI GCTCTTC 1 cut(s) 357
PctI GAATGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 2 cut(s) 319, 616
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 4 cut(s) 337, 445, 448, 624
PshBI ATTAAT 1 cut(s) 465
PsiI TTATAA 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 114
PspGI CCWGG 1 cut(s) 114
PspN4I GGNNCC 1 cut(s) 428
PstI CTGCAG 4 cut(s) 58, 288, 422, 624
PsuI RGATCY 1 cut(s) 501
PvuII CAGCTG 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 437
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 3 cut(s) 465, 495, 578
SatI GCNGC 4 cut(s) 336, 444, 447, 623
Sau3AI GATC 2 cut(s) 501, 534
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 1 cut(s) 431
SetI ASST 8 cut(s) 70, 117, 129, 369, 380, 448, 558, 578
SfaNI GCATC 1 cut(s) 568
SfcI CTRYAG 4 cut(s) 54, 284, 418, 620
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 257, 516
SmoI CTYRAG 2 cut(s) 257, 516
Sse9I AATT 2 cut(s) 81, 267
SsiI CCGC 1 cut(s) 387
SspI AATATT 1 cut(s) 227
SspMI CTAG 2 cut(s) 569, 628
StyD4I CCNGG 1 cut(s) 114
TaqI TCGA 1 cut(s) 322
TasI AATT 2 cut(s) 81, 267
TfiI GAWTC 2 cut(s) 319, 616
Tru1I TTAA 3 cut(s) 465, 495, 578
Tru9I TTAA 3 cut(s) 465, 495, 578
TscAI CASTG 2 cut(s) 58, 67
TseI GCWGC 4 cut(s) 335, 443, 446, 622
TspDTI ATGAA 1 cut(s) 91
TspRI CASTG 2 cut(s) 58, 67
Van91I CCANNNNNTGG 1 cut(s) 346
VspI ATTAAT 1 cut(s) 465
XmnI GAANNNNTTC 1 cut(s) 590
XspI CTAG 2 cut(s) 569, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.